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General José de San Martín, Argentina

Caracciolo B.,Institute Fisiologia y Bioquimica Clinica | Muzietti S.,Institute Fisiologia y Bioquimica Clinica | Pandolfo M.,Institute Fisiologia y Bioquimica Clinica | Negri G.,Institute Fisiologia y Bioquimica Clinica | Bustos D.,Institute Fisiologia y Bioquimica Clinica
Acta Bioquimica Clinica Latinoamericana

The aim of this study was to determine the Biological Variation (BV) of Aldolase (ALD) in healthy patients, the individuality index (II) of the analyte and to assess how useful the population reference interval (PRI) is for the interpr etation of the results, considering PRI useful when the II is >1.40 and useless when it is <0.6. Reference change values (RCV) were also assessed in the interpretation of two serial measurements of the same individual, specially in the monitoring of patients in which the serum level of Creatine Kinase (CK) remain within the reference range while ALD values are increased. Eight blood samples were obtained from ten apparently healthy subjects in different days according to a predetermined protocol. ALD activity was measured by an enzymatic UV method and BV, II and RCV were calculated. Intraindividual BV was 31%, interindividual BV 21%, II 1.47 and RCV 90%. Based on the results obtained for BV and II, PRIs are useful for the interpretation of ADL results. A serial result will be statistically different from the previous one when the variability between both results is higher than 90%. RCV must be determined in each laboratory using their own analytical variability in order to obtain a correct interpretation of the results. Source

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