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Arencibia-Arrebola D.F.,Instituto Finlay | Rosario-Fernandez L.A.,Institute Farmacia Y Alimentos IFAL | Suarez-Fernandez Y.E.,Agricultural University of Havana
BAG - Journal of Basic and Applied Genetics | Year: 2011

The aim of this study was to evaluate the sensibility of different murine lines to the cyclophosphamide (CF) measure through the micronucleus assay in bone marrow cells. Being used mice of both sexes of the Balb/c, NMRI, OF-1 and C57BL/6/cenp lines. The use of the best line was suggested in this assay on the base of significant appearance of low indexes spontaneous and high induced indexes to the CF. It was obtained as a result that the Balb/c line in both sexes differs significantly of the other evaluated lines. In this line the lowest results in cytotoxicity index (relationship among young erythrocytes/mature erythrocytes) and genotoxicity indexes (erythrocytes percent in bone marrow with micronucleus) were observed, at the same time of responding from an acceptable form to the CF. Also the line C57/BL6/cenp was the less efficient and more sensitive to the mutagen, being obtained the highest spontaneous and induced results. These results allow to suggest that the Balb/c mice, is the most appropriate line to use as biomodel in the micronucleus assay, when it is used the CF as positive control.

Daniel Arencibia A.,Instituto Finlay | Luis Rosario F.,Institute Farmacia Y Alimentos IFAL
Revista MVZ Cordoba | Year: 2012

Objective: The aim of this study was to compare spontaneous and induced rates in mice of both sexes of lines BALB/c, NMRI, OF-1 and C57BL/6/ cenp, by testing chromosomal aberrations in bone marrow cells. Materials and methods: For this purpose, the most efficient line was determined, based on the significant appearance of lower spontaneous rates and higher induced rates to mutagen substances such as cyclophosphamide (CP). Results: The result obtained was that line Balb/c, in both sexes, differs significantly from the other lines tested. The lesser total number of cells with structural aberrations was found in this line and a greater number of induction of chromosomal aberrations when CP was used as a mutagen. Conclusions: This work will give way to the use of the best line of mice as bio model in this genotoxicity test, and will also give this cytogenetic technique greater veracity and robustness.

Luis Rosario F.,Institute Farmacia Y Alimentos IFAL | Daniel Arencibia A.,Instituto Finlay | Niurka Batista S,Instituto Finlay | Juan Infante B.,Instituto Finlay
Revista MVZ Cordoba | Year: 2013

The aim of this study was to characterize the symptomatology and anatomopathological lesions caused by 5 clinical isolated Leptospira spp. from Nicaragua in a Mesocricetus auratus biomodel. Materials and methods. 50 hamsters were inoculated via i.p with 1mL of the culture of each strain in exponential phase having a cellular concentration of 7.5 x 106 leptospira/mL, (10 animals per strain). Signs of the disease, mortality during 14 days, and macroscopic and microscopic anatomopathological lesions by haematoxylin-eosin and Warthyn Starryn stain technique were evaluated. Results. All the strains presented high mortality, showing clinical lesions of the experimental infection. Death to 100% of the animals was caused between the third and tenth day post-infection. In the anatomopathologic study, the strains of the Ballum and Pomona serogroup produced haemorrhaging specifically in the kidney and lungs. The animals manifested hepatic and renal congestion, while the renal haemorrhage was observed with more frequency in the strain of the Pomona serogroup, differing from the other strains, which presented this lesion less frequently. Conclusions. This work allowed a better characterization of these strains in order to use them as future vaccine candidates for future Leptospirosis epidemics in Nicaragua.

Leon R.,NeuroLogica | Almaguer W.,NeuroLogica | Marin J.,Institute Farmacia Y Alimentos IFAL | Lorigados L.,NeuroLogica | And 5 more authors.
Acta Biologica Colombiana | Year: 2015

Cerebral ischemia is a major cause of death, for this reason animal models of cerebral ischemia are widely used to study both the pathophysiology of ischemic phenomenon and the evaluation of possible therapeutic agents with protective or regenerative properties. The objectives of this study were to examine the presence of neuronal damage in different brain areas following the ischemic event, and assess consequences of such activities on the processes of memory and learning. The study group included an experimental group ischemic animals (30 rats with permanent bilateral occlusion of the carotids), and a control group. Was evaluated gene expression and inflammatory ischemic by qPCR techniques 24h post injury, brain tissue morphology in areas of cortex, striatum and hippocampus seven days post injury and processes of memory and learning, 12 days post injury. The morphological studies showed that the procedure induces death of cell populations in cortex, striatum and hippocampus, ischemia modified gfap gene expression and ho, il-6, il-17 and ifn-γ, which can be used as a marker of early ischemic process. Additionally, the ischemic injury caused spatial memory decline. This characterization gives us an experimental model to develop future studies on the pathophysiology of ischemic events and assessing therapeutic strategies. © 2015, Universidad Nacional de Colombia. All rights reserved.

Arencibia-Arrebola D.F.,Instituto Finlay | Rosario-Fernandez L.A.,Institute Farmacia Y Alimentos IFAL | Suarez-Fernandez Y.E.,Agricultural University of Havana
Revista Internacional de Andrologia | Year: 2011

Objective: This article has aimed to compare Balb/c, NMRI, OF-1 and C57BL/6/cenp mice as a biomodel in the assay of the sperm head basal cavity morphology, keeping in mind the frequency of anomalous forms of the basal head and cyclophosphamide-induced (CF) abnormalities. Materials and methods: Twenty animals/group/mice lines were used, administered for 35 days. A negative control group (not administered), two substance-vehicle controls and a positive control administered with 50. mg/kg FG intraperitoneally for five consecutive days were used. Results and discussion: The best experimental biomodel was with the Balb/c mice, with significant difference with the results obtained in the other lines, keeping in mind the spontaneous and induced values in the sperm concentration and frequency of anomalous epididymal sperms. This study will make it possible to use this mice species more efficiently in genotoxic and preclinical toxicology fertility evaluation of drugs, vaccines and other products. © 2011 Sociedad Española de Andrología.

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