Institute Ensino e Pesquisa da Santa Casa

Belo Horizonte, Brazil

Institute Ensino e Pesquisa da Santa Casa

Belo Horizonte, Brazil

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De Souza A.H.,Lutheran University of Brazil | Da Costa Lopes A.M.,Institute Ensino e Pesquisa da Santa Casa | Castro Jr. C.J.,Institute Ensino e Pesquisa da Santa Casa | Pereira E.M.R.,Federal University of Minas Gerais | And 6 more authors.
Toxicon | Year: 2014

This study investigated the effects of Phα1β, pregabalin and diclofenac using an animal model of fibromyalgia (FM). Repeated administration of reserpine (0.25 mg/kg sc) once daily for three consecutive days significantly decreased thermal hyperalgesia, mechanical allodynia, and dopamine and serotonin content in the brain on the 4th day. Phα1β and pregabalin treatment completely reverted the mechanical allodynia and thermal hyperalgesia induced by reserpine treatment on the 4th day, but diclofenac was ineffective. Reserpine treatment significantly increased the immobility time in the forced swim test, which is indicative of depression in the animals. Phα1β, but not pregabalin, reduced the immobility time (56%), suggesting that Phα1β may control persistent pathological pain in FM. © 2014 Elsevier B.V.


PubMed | Lutheran University of Brazil, Grande Rio University, Federal University of Santa Maria, Federal University of Minas Gerais and Institute Ensino e Pesquisa da Santa Casa
Type: | Journal: Toxicon : official journal of the International Society on Toxinology | Year: 2014

This study investigated the effects of Ph1, pregabalin and diclofenac using an animal model of fibromyalgia (FM). Repeated administration of reserpine (0.25 mg/kg sc) once daily for three consecutive days significantly decreased thermal hyperalgesia, mechanical allodynia, and dopamine and serotonin content in the brain on the 4th day. Ph1 and pregabalin treatment completely reverted the mechanical allodynia and thermal hyperalgesia induced by reserpine treatment on the 4th day, but diclofenac was ineffective. Reserpine treatment significantly increased the immobility time in the forced swim test, which is indicative of depression in the animals. Ph1, but not pregabalin, reduced the immobility time (56%), suggesting that Ph1 may control persistent pathological pain in FM.


Garcia D.P.C.,Institute Ensino e Pesquisa da Santa Casa | Santos Neto C.,Hospital Universitario Sao Jose | Hubner P.N.V.,Hospital Felicio Rocho | Furtado T.A.,Hospital Felicio Rocho | And 3 more authors.
Acta Cirurgica Brasileira | Year: 2016

PURPOSE: To develop an experimental model for incisional hernias and to compare morphological and functional aspects of hernia repairs by suture, polypropylene mesh and collagen mesh. METHODS: A defect measuring 7cm x 2cm was created in the anterior abdominal of 28 New Zealand male rabbits, divided into four groups (n = 7): (1) control, (2) suture of the anterior sheath of the rectus abdominal muscle, (3) setting of polypropylene mesh, and (4) setting of collagen mesh. On the 90th postoperative day, the animals were examined to verify the presence of incisional hernia. Samples of abdominal wall and scar were collected for histological study. RESULTS: Incisional hernia was identified in 85.7% of the control group, 57.1% of the suture group, 42.9% of the collagen mesh group, and none in the polypropylene mesh group (p = 0.015). Mesh exposure could be identified in 71.4% of the animals in group 3 and in no animal in group 4 (p = 0.021). The polypropylene mesh is effective in the treatment of abdominal wall defects, causing an intense inflammatory reaction. CONCLUSION: The collagen mesh is biocompatible, producing a minimal inflammatory reaction, but fails in the treatment of abdominal wall defects. © 2016,Sociedade Brasileira para o Desenvolvimento de Pesquisa em Cirurgia. All Rights reserved.


Diniz D.M.,Institute Ensino e Pesquisa da Santa Casa | De Souza A.H.,Lutheran University of Brazil | Pereira E.M.R.,Federal University of Minas Gerais | Da Silva J.F.,Institute Ensino e Pesquisa da Santa Casa | And 8 more authors.
Pharmacology Biochemistry and Behavior | Year: 2014

The effects of intrathecal administration of the toxins Phα1β and ω-conotoxin MVIIA were investigated in visceral nociception induced by an intraperitoneal injection of acetic acid and an intracolonic application of capsaicin. The pretreatments for 2 h with the toxins reduced the number of writhes or nociceptive behaviors compared with the control mice. Phα1β administration resulted in an Imax of 84 ± 6 and an ID50 of 12 (5-27), and ω-conotoxin MVIIA resulted in an Imax of 82 ± 9 and an ID50 of 11 (4-35) in the contortions induced by the intraperitoneal injection of acetic acid. The administration of Phα1β resulted in an Imax of 64 ± 4 and an ID50 of 18 (9-38), and ω-conotoxin MVIIA resulted in an Imax of 71 ± 9 and an ID50 of 9 (1-83) in the contortions induced by intracolonic capsaicin administration. Phα1β (100/site) or ω-conotoxin MVIIA (30 pmol/site) pretreatments caused a reduction in CSF glutamate release in mice intraperitoneally injected with acetic acid or treated with intracolonic capsaicin. The toxin pretreatments reduced the ROS levels induced by intraperitoneal acetic acid injection. Phα1β, but not ω-conotoxin MVIIA, reduced significantly the ROS levels induced by intracolonic capsaicin administration.


PubMed | Lutheran University of Brazil, Fundacao Ezequiel Dias FUNED, Federal University of Santa Maria, Federal University of Minas Gerais and Institute Ensino e Pesquisa da Santa Casa
Type: | Journal: Pharmacology, biochemistry, and behavior | Year: 2014

The effects of intrathecal administration of the toxins Ph1 and -conotoxin MVIIA were investigated in visceral nociception induced by an intraperitoneal injection of acetic acid and an intracolonic application of capsaicin. The pretreatments for 2h with the toxins reduced the number of writhes or nociceptive behaviors compared with the control mice. Ph1 administration resulted in an Imax of 846 and an ID50 of 12 (5-27), and -conotoxin MVIIA resulted in an Imax of 829 and an ID50 of 11 (4-35) in the contortions induced by the intraperitoneal injection of acetic acid. The administration of Ph1 resulted in an Imax of 644 and an ID50 of 18 (9-38), and -conotoxin MVIIA resulted in an Imax of 719 and an ID50 of 9 (1-83) in the contortions induced by intracolonic capsaicin administration. Ph1 (100/site) or -conotoxin MVIIA (30pmol/site) pretreatments caused a reduction in CSF glutamate release in mice intraperitoneally injected with acetic acid or treated with intracolonic capsaicin. The toxin pretreatments reduced the ROS levels induced by intraperitoneal acetic acid injection. Ph1, but not -conotoxin MVIIA, reduced significantly the ROS levels induced by intracolonic capsaicin administration.Ph1 is a -toxin with high therapeutic index and a broader action on calcium channels. It shows analgesic effect in several rodents models of pain, including visceral pain, suggesting that this toxin has the potential to be used in clinical setting as a drug in the control of persistent pathological pain.


de Figueiredo L.O.,Hospital Felicio Rocho | Garcia D.P.C.,Institute Ensino e Pesquisa da Santa Casa | Alberti L.R.,FMUFMG and Institute Ensino e Pesquisa da Santa Casa | Paiva R.A.,Colorectal Surgeon at Hospital Felicio Rocho | And 3 more authors.
International Journal of Surgery Case Reports | Year: 2016

Introduction Intussusception in adult is rarely caused by idiopathic conditions. Main causes are inflammatory diseases, benign or malignant tumors and motility disorders. As a benign cause, lipomas appear as a particularly rare gastrointestinal intraluminal tumor occurring with highest incidence in the colon, mostly in the caecum and ascending colon. Presentation of case A 57-year-old male patient was admitted at the surgical emergency in Belo Horizonte, with history of chronic and intermittent diffuse abdominal pain, associated with variations of his bowel habits and rare episodes of vomiting starting around 3 days prior to admission. Discussion Intussusception is the cause of adult symptomatic bowel obstruction in 1% of the cases and its colocolonic occurrence represents 17% of all intestinal intussusceptions in adults. The reported case presents itself as even rarer considering its evaluation according to the epidemiological statistics of 1:5 men/women ratio and lipoma's most common location being the right colon. Intussusception and intestinal obstructions caused by intraluminal lipomas are not often described in the literature and its occurrence is directly related to its size, usually larger than 2 cm diameter. The management of lipomatous intraluminal lesions of the colon is traditionally surgical, and it allows a selective resection, depending on the size of the tumor, length of intussusception, and the amount of inflammation. Conclusion Patients with chronic abdominal symptoms and semi-obstruction caused by intussusception are rarely diagnosed before surgery unless there is a high index of suspicion. Colonoscopy contributes to diagnosis given that it provides direct visualization and biopsy. © 2016 The Authors

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