São Paulo, Brazil
São Paulo, Brazil

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Kihara A.H.,Federal University of ABC | Kihara A.H.,University of Sao Paulo | Santos T.O.,University of Sao Paulo | Osuna-Melo E.J.,University of Sao Paulo | And 9 more authors.
International Journal of Developmental Neuroscience | Year: 2010

Connexin (Cx) channels and hemichannels are involved in essential processes during nervous system development such as apoptosis, propagation of spontaneous activity and interkinetic nuclear movement. In the first part of this study, we extensively characterized Cx gene and protein expression during retinal histogenesis. We observed distinct spatio-temporal patterns among studied Cx and an overriding, ubiquitous presence of Cx45 in progenitor cells. The role of Cx-mediated communication was assessed by using broad-spectrum (carbenoxolone, CBX) and Cx36/Cx50 channel-specific (quinine) blockers. In vivo application of CBX, but not quinine, caused remarkable reduction in retinal thickness, suggesting changes in cell proliferation/apoptosis ratio. Indeed, we observed a decreased number of mitotic cells in CBX-injected retinas, with no significant changes in the expression of PCNA, a marker for cells in proliferative state. Taken together, our results pointed a pivotal role of Cx45 in the developing retina. Moreover, this study revealed that Cx-mediated communication is essential in retinal histogenesis, particularly in the control of cell proliferation. © 2009 ISDN.


Bonamin L.V.,Paulista University | Bonamin L.V.,University of Santo Amaro | Bonamin L.V.,Institute Ensino | Sato C.,Paulista University | And 13 more authors.
Evidence-based Complementary and Alternative Medicine | Year: 2013

The present study analyzed the immune modulation mechanisms of thymulin 5CH in a granuloma experimental model. Male adult Balb/c mice were inoculated with BCG into the footpad to induce granuloma, which was quantitatively evaluated. The phenotypic characterization of phagocyte, T- and B-lymphocyte populations in the peritoneum, and local lymph node was done by flow cytometry. During all experimental periods, thymulin 5CH and vehicle (control) were given ad libitum to mice, diluted into the drinking water (1.6×10-17 M). After 7 days from inoculation, thymulin-treated mice presented reduction in the number of epithelioid cytokeratine-positive cells (P=0.0001) in the lesion, in relation to young phagocytes. After 21 days, the differentiation of B1 peritoneal stem cells into phagocytes reached the peak, being higher in thymulin-treated mice (P=0.0001). Simultaneously, the score of infected phagocytes in the lesion decreased (P=0.001), and the number of B1-derived phagocytes, CD4+ and CD8+ T lymphocytes in the local lymph node increased in relation to control (P=0.0001). No difference was seen on the CD25+ Treg cells. The results show that thymulin 5CH treatment is able to improve the granuloma inflammatory process and the infection remission, by modulating local and systemic phagocyte differentiation. © 2013 Leoni Villano Bonamin et al.


De Deus Santos M.R.,Institute Ensino | Martins A.S.,Federal University of Espirito Santo | Baptistotte C.,Projeto TAMAR ICMBio | Work T.M.,United States Geological Survey National Wildlife Health Center Honolulu Field Station
Diseases of Aquatic Organisms | Year: 2015

Packed cell volume (PCV), plasma biochemistry, visual body condition (BC), and calculated body condition index (BCI) were evaluated in 170 wild juvenile green sea turtles Chelonia mydas from an aggregation in the effluent canal of a steel mill in Brazil. Occurrence of cutaneous fibropapillomatosis (FP) was observed in 44.1% of the animals examined. BCI alone did not differ significantly between healthy animals and those afflicted with FP. However, all turtles with low BCI were severely afflicted and were uremic, hypoglycemic, and anemic in relation to healthy animals. Severe FP was not always reflected by a poor health condition of the individual. Clinical evaluation and plasma biochemistry indicated that most animals afflicted with FP were in good health condition. Differences in FP manifestations and associated health conditions in different geographic regions must be assessed by long-term health monitoring programs to help define priorities for conservation efforts. © 2015 Inter-Research.


Benvegnu L.,Institute Ensino | Maffessoni M.,Institute Ensino | Fernandes S.P.,Federal University of Rio Grande do Sul | Canuto R.,Federal University of Rio Grande do Sul
Scientia Medica | Year: 2016

Aims: To study the association between sleep deprivation and obesity among workers. Methods: We conducted a cross-sectional study of workers at a notebooks manufacturing in southern Brazil, which has three sectors of work (production line, back office and logistics), and the production sector works 24 hours a day. Demographic, socioeconomic, occupational and behavioral characteristics were obtained by a self-completion questionnaire, standardized and pre-tested. Weight and height measurements were self-reported and body mass index calculated. The cutoff point for obesity was ≥30 kg/m2. Sleep duration was categorized into at least six hours a day and less than six hours a day (sleep deprivation). For bivariate analysis, chi-square test and test for linear trend were used. The crude and adjusted odds ratios were calculated using Poisson regression. Results: The final sample consisted of 193 workers, with a mean age of 34±11 years, 99 (51%) women. The prevalence of obesity was 27 (13.98%), and the prevalence of sleep deprivation was 40 (20.7%). Sleep deprivation has been associated with the production sector (p=0.011), the night shift (p<0.001) and physical inactivity (p=0.003). The presence of obesity was associated with sleep deprivation (p=0.006). After controlling for potential confounding factors, the likelihood of being obese was 2.37 (95% CI 1.08 to 5.21) times higher among workers in sleep deprivation. Conclusions: The results showed an association between sleep deprivation and obesity. In addition, sleep deprivation was associated with work on the night shift.


Santos R.S.,Boston University | Santos R.S.,Institute Ensino | Gupta A.,Boston University | Ebright M.I.,Boston University | And 5 more authors.
Annals of Thoracic Surgery | Year: 2010

Purpose: We evaluated an electromagnetic (EM) navigation system (Veran Medical Technologies Inc, St. Louis, MO) to determine its potential to reduce the number of skin punctures and instrument adjustments during computed tomographic-guided percutaneous ablation and biopsy of lung nodules. Description: Ten patients undergoing lung percutaneous ablation were prospectively enrolled. The mean age was 70 years. Positioning of the needle device was verified with computed tomographic fluoroscopy prior to the execution of any biopsy or ablation. Each EM navigation-guided procedure was defined as an EM-intervention. Evaluation: Nineteen EM interventions were performed. When an EM-guided biopsy was performed, the intervention was done immediately prior to ablation. For all 19 EM interventions, only one skin-puncture was required. The mean number of instrument adjustments required was 1.2 (range, 0 to 2). The mean time for each EM intervention was 5.2 minutes (range, 1 to 20 minutes). Pneumothorax occurred in 5 patients (50%). Only the number of instrument adjustments was significantly related to the pneumothorax rate (p = 0.005). Conclusions: The EM navigation is feasible and seems to be a useful aid for image-guided procedures. Early experience suggests a low number of skin-puncture and instrument adjustments using the EM navigation system. Instrument adjustments were a key factor in pneumothorax development. © 2010 The Society of Thoracic Surgeons.


Junior L.S.,Institute Ensino
Algorithmic Finance | Year: 2013

Using data from world stock exchange indices prior to and during periods of global financial crises, clusters and networks of indices are built for asset graphs based on distance thresholds and diverse periods of time, so that it is then possible to analyze how clusters are formed according to correlations among indices and how they evolve in time, particularly during times of financial crises. Further analysis is made on the eigenvectors corresponding to the second highest eigenvalues of the correlation matrices, revealing a structure peculiar to markets that operate in different time zones. We also study the survivability of connections and of clusters through time and the influence of noise in centrality measures applied to the networks of financial indices. The results show how the world's main stock market indices evolved in the last few decades with respect to their clustering structure, how their connections survive in time, and which indices are more central, according to different criteria. In particular, we witness the early formation and evolution of two main clusters, an American and an European one, the formation of a Pacific Asian cluster, and later on, of an Arab cluster. This analysis complements previous studies of the interdependencies of stock markets worldwide. © 2013 - IOS Press and the authors.


PubMed | Institute Ensino
Type: Journal Article | Journal: Diseases of aquatic organisms | Year: 2015

Packed cell volume (PCV), plasma biochemistry, visual body condition (BC), and calculated body condition index (BCI) were evaluated in 170 wild juvenile green sea turtles Chelonia mydas from an aggregation in the effluent canal of a steel mill in Brazil. Occurrence of cutaneous fibropapillomatosis (FP) was observed in 44.1% of the animals examined. BCI alone did not differ significantly between healthy animals and those afflicted with FP. However, all turtles with low BCI were severely afflicted and were uremic, hypoglycemic, and anemic in relation to healthy animals. Severe FP was not always reflected by a poor health condition of the individual. Clinical evaluation and plasma biochemistry indicated that most animals afflicted with FP were in good health condition. Differences in FP manifestations and associated health conditions in different geographic regions must be assessed by long-term health monitoring programs to help define priorities for conservation efforts.

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