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Ippoliti C.,Instituto Zooprofilattico Sperimentale Dellabruzzo E Del Molise G Caporale | Calistri P.,Instituto Zooprofilattico Sperimentale Dellabruzzo E Del Molise G Caporale | Blanco P.,Institute Ecologia y Sistematica IES | Conte A.,Instituto Zooprofilattico Sperimentale Dellabruzzo E Del Molise G Caporale | And 3 more authors.
Preventive Veterinary Medicine | Year: 2014

The authors designed a risk-based approach to the selection of poultry flocks to be sampled in order to further improve the sensitivity of avian influenza (AI) active surveillance programme in Cuba. The study focused on the western region of Cuba, which harbours nearly 70% of national poultry holdings and comprise several wetlands where migratory waterfowl settle (migratory waterfowl settlements - MWS). The model took into account the potential risk of commercial poultry farms in western Cuba contracting from migratory waterfowl of the orders Anseriformes and Charadriiformes through dispersion for pasturing of migratory birds around the MWS. We computed spatial risk index by geographical analysis with Python scripts in ESRI® ArcGIS 10 on data projected in the reference system NAD 1927-UTM17. Farms located closer to MWS had the highest values for the risk indicator pj and in total 31 farms were chosen for targeted surveillance during the risk period. The authors proposed to start active surveillance in the study area 3 weeks after the onset of Anseriformes migration, with additional sampling repeated twice in the same selected poultry farms at 15 days interval (Comin et al., 2012; EFSA, 2008) to cover the whole migration season. In this way, the antibody detectability would be favoured in case of either a posterior AI introduction or enhancement of a previous seroprevalence under the sensitivity level. The model identified the areas with higher risk for AIV introduction from MW, aiming at selecting poultry premises for the application of risk-based surveillance. Given the infrequency of HPAI introduction into domestic poultry populations and the relative paucity of occurrences of LPAI epidemics, the evaluation of the effectiveness of this approach would require its application for several migration seasons to allow the collection of sufficient reliable data. © 2014 The Authors.


PubMed | Institute Medicina Veterinaria, Instituto Zooprofilattico Sperimentale Dellabruzzo E Del Molise G Caporale and Institute Ecologia y Sistematica IES
Type: Journal Article | Journal: Preventive veterinary medicine | Year: 2014

The authors designed a risk-based approach to the selection of poultry flocks to be sampled in order to further improve the sensitivity of avian influenza (AI) active surveillance programme in Cuba. The study focused on the western region of Cuba, which harbours nearly 70% of national poultry holdings and comprise several wetlands where migratory waterfowl settle (migratory waterfowl settlements - MWS). The model took into account the potential risk of commercial poultry farms in western Cuba contracting from migratory waterfowl of the orders Anseriformes and Charadriiformes through dispersion for pasturing of migratory birds around the MWS. We computed spatial risk index by geographical analysis with Python scripts in ESRI() ArcGIS 10 on data projected in the reference system NAD 1927-UTM17. Farms located closer to MWS had the highest values for the risk indicator pj and in total 31 farms were chosen for targeted surveillance during the risk period. The authors proposed to start active surveillance in the study area 3 weeks after the onset of Anseriformes migration, with additional sampling repeated twice in the same selected poultry farms at 15 days interval (Comin et al., 2012; EFSA, 2008) to cover the whole migration season. In this way, the antibody detectability would be favoured in case of either a posterior AI introduction or enhancement of a previous seroprevalence under the sensitivity level. The model identified the areas with higher risk for AIV introduction from MW, aiming at selecting poultry premises for the application of risk-based surveillance. Given the infrequency of HPAI introduction into domestic poultry populations and the relative paucity of occurrences of LPAI epidemics, the evaluation of the effectiveness of this approach would require its application for several migration seasons to allow the collection of sufficient reliable data.


Dominguez M.,CSIC - National Museum of Natural Sciences | Dominguez M.,University of Alicante | Fong A.,Centro Oriental Of Ecosistemas Y Biodiversidad Bioeco | Iturriaga M.,Institute Ecologia y Sistematica IES
Zootaxa | Year: 2013

Typhlops leptolepis sp. nov. is a new blind snake from Holguín Province, northeastern region of Cuba. The new species is characterized by its small and thin body size, as well as a narrow rostral in dorsal view, longer than broad, with parallel to curved sides, tapering toward anterior tip. The preocular is in contact with third supralabial only. It has 20 anterior scale rows reducing to 18 posteriorly at around midbody or posterior to midbody, moderate to high middorsal scale counts (250- 308), and a peculiar coloration (head, neck, and tail whitish spotted in ventral view). Based on its morphology, the new species can be placed within the Typhlops lumbricalis species group and a key to the species belonging to this group is presented. Copyright © 2013 Magnolia Press.


Daniel H.-M.,Catholic University of Leuven | Rosa C.A.,Federal University of Minas Gerais | Thiago-Calaca P.S.S.,Laboratorio Of Recursos Vegetais E Opoterapicos | Antonini Y.,Federal University of Ouro Preto | And 6 more authors.
International Journal of Systematic and Evolutionary Microbiology | Year: 2013

A novel yeast species was found repeatedly and in high cell densities in underground-nesting stingless bees of the species Melipona quinquefasciata and their provisions in northern Minas Gerais (Brazil). One additional strain was isolated from bee-collected pollen in Cuba. Phylogenetic analyses based on rRNA gene sequences (D1/D2 large subunit gene and internal transcribed spacer) indicated that the novel species belongs to the Starmerella clade and is most closely related to Candida (iter. nom. Starmerella) apicola. Growth reactions on carbon and nitrogen sources were typical of those observed in related species of the Starmerella clade. PCRfingerprinting with mini- and microsatellite specific primers allowed the distinction of the novel species from Candida apicola, Candida bombi and a yet undescribed species represented by strain CBS 4353. On the basis of phylogenetic relationships, the novel species is assigned to the genus Starmerella despite the failure to observe sexual reproduction after extensive mating tests. We propose the name Starmerella neotropicalis f. a., sp. nov. (Mycobank MB 804285) and designate UFMG PST 09T (=MUCL 53320T=CBS 12811T) as the type strain. © 2013 IUMS.

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