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Cognet L.,University of Bordeaux 1 | Cognet L.,Institute dOptique and oCNRS | Leduc C.,Institute Pasteur Paris | Lounis B.,University of Bordeaux 1 | Lounis B.,Institute dOptique and oCNRS
Current Opinion in Chemical Biology | Year: 2014

Resolving the movement of individual molecules in living cells by single particle tracking methods has allowed many molecular behaviors to be deciphered over the past three decades. These methods have increasingly benefited from advances in microscopy of single nano-objects such as fluorescent dye molecules, proteins or nanoparticles as well as tiny absorbing metal nanoparticles. In parallel to these efforts aiming at tracking ever smaller and more photostable nano-objects in living cells, the development of localization-based super-resolution imaging provided means to increase the number of single molecules tracked on a single cell. In this review we will present the most recent advances in the field. © 2014 Elsevier Ltd.

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