Institute Diagnostico y Referencia Epidemiologicos InDRE

Mexico City, Mexico

Institute Diagnostico y Referencia Epidemiologicos InDRE

Mexico City, Mexico
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De La Cruz Hernandez S.I.,Institute Diagnostico y Referencia Epidemiologicos InDRE | De La Cruz Hernandez S.I.,National Polytechnic Institute of Mexico | Gonzalez-Mateos S.,Institute Diagnostico y Referencia Epidemiologicos InDRE | Lopez-Martinez I.,Institute Diagnostico y Referencia Epidemiologicos InDRE | And 3 more authors.
Journal of Clinical Virology | Year: 2013

Background: The measurement and detection of viremia and antigenemia in sera have been used as a marker of risk for dengue disease severity and diagnosis. However, evidence exists suggesting that levels of viremia and antigenemia are affected by the presence of specific antibodies. Objective: To compare viral load and circulating NS1 levels in sera from patients positive or negative for dengue specific IgM antibodies. Study design: Three hundred and eighty serum samples were analyzed for viral load using qRT-PCR and for levels of circulating NS1 and the presence of specific antibodies using commercial EIAs. Results and conclusion: Comparison of viremia levels in sera from patients positive or negative for dengue IgM antibodies showed that viral load was significantly higher ( p≤. 0.0001) in patients negative for IgM antibodies. In contrast, levels of circulating NS1 were found unaffected by the presence of IgM ( p= 0.0038). Thus, dengue virus specific IgM antibodies in sera seem to be a strong negative modulator of viremia levels in patient's sera. © 2013 Elsevier B.V.

PubMed | Autonomous University of Yucatán, Institute Diagnostico y Referencia Epidemiologicos InDRE, Autonomous University of Guerrero, Emory University and Servicios Estatales de Salud de Guerrero
Type: | Journal: Journal of medical entomology | Year: 2016

The markedly anthropophilic and endophilic behaviors of Aedes aegypti (L.) make it a very efficient vector of dengue, chikungunya, and Zika viruses. Although a large body of research has investigated the immature habitats and conditions for adult emergence, relatively few studies have focused on the indoor resting behavior and distribution of vectors within houses. We investigated the resting behavior of Ae. aegypti indoors in 979 houses of the city of Acapulco, Mexico, by performing exhaustive indoor mosquito collections to describe the rooms and height at which mosquitoes were found resting. In total, 1,403 adult and 747 female Ae. aegypti were collected, primarily indoors (98% adults and 99% females). Primary resting locations included bedrooms (44%), living rooms (25%), and bathrooms (20%), followed by kitchens (9%). Aedes aegypti significantly rested below 1.5 m of height (82% adults, 83% females, and 87% bloodfed females); the odds of finding adult Ae. aegypti mosquitoes below 1.5 m was 17 times higher than above 1.5 m. Our findings provide relevant information for the design of insecticide-based interventions selectively targeting the adult resting population, such as indoor residual spraying.

PubMed | Centers for Disease Control and Prevention, University of the Valley of Guatemala, Institute Diagnostico y Referencia Epidemiologicos InDRE, University of the West Indies and 6 more.
Type: Journal Article | Journal: Influenza and other respiratory viruses | Year: 2016

Influenza-associated illness results in increased morbidity and mortality in the Americas. These effects can be mitigated with an appropriately chosen and timed influenza vaccination campaign. To provide guidance in choosing the most suitable vaccine formulation and timing of administration, it is necessary to understand the timing of influenza seasonal epidemics.Our main objective was to determine whether influenza occurs in seasonal patterns in the American tropics and when these patterns occurred.Publicly available, monthly seasonal influenza data from the Pan American Health Organization and WHO, from countries in the American tropics, were obtained during 2002-2008 and 2011-2014 (excluding unseasonal pandemic activity during 2009-2010). For each country, we calculated the monthly proportion of samples that tested positive for influenza. We applied the monthly proportion data to a logistic regression model for each country.We analyzed 2002-2008 and 2011-2014 influenza surveillance data from the American tropics and identified 13 (81%) of 16 countries with influenza epidemics that, on average, started during May and lasted 4 months.The majority of countries in the American tropics have seasonal epidemics that start in May. Officials in these countries should consider the impact of vaccinating persons during April with the Southern Hemisphere formulation.

Morrison B.A.,HUMIGEN LLC | Ucisik-Akkaya E.,HUMIGEN LLC | Flores H.,Institute Diagnostico y Referencia Epidemiologicos InDRE | Alaez C.,Institute Diagnostico y Referencia Epidemiologicos InDRE | And 2 more authors.
Autoimmunity | Year: 2010

Previous epidemiologic studies showed four times increased risk of acute lymphoblastic leukemia (ALL) in children of women with multiple sclerosis (MS). MS shows a risk association with Human leukocyte antigens (HLA)-DRA single nucleotide polymorphism (SNP) rs3135388, which is a proxy marker for DRB1*1501. We examined the relevance of rs3135388 in childhood ALL risk along with two other HLA-DRA SNPs in two casecontrol groups: 114 cases and 388 controls from South Wales (UK) and 100 Mexican Mestizo cases and 253 controls. We first confirmed the correlation between rs3135388 and DRB1*1501 in HLA-typed reference cell lines. We noted a female-specific risk association in childhood ALL (pooled odds ratio (OR) =2.6, 95% confidence interval (CI) =1.5-4.5, MantelHaenszel P =0.0009) similar to the stronger association of DRB1*1501 in females with MS. Examination of an HLA-C 5′ flanking region SNP rs9264942, known to correlate with HLA-C expression, showed a protective association in girls (OR 0.4, 95% CI =0.2-0.7, MantelHaenszel P 0.0003) similar to the protective HLA-Cw*05 association in MS. In a reference cell line panel, HLA-Cw5 homozygous samples (n 8) were also homozygous for the minor allele of the SNP. Likewise, the male-specific protective association of interferon-gamma (IFNG) SNP rs2069727 in MS was replicated with the same sex specificity in childhood ALL (OR 0.6, 95% CI =0.4-1.0, MantelHaenszel P =0.03). Two other SNPs in superkiller viralicidic activity 2-like and tenascin XB that are markers for systemic lupus erythematosus susceptibility showed female-specific associations but due to linkage disequilibrium with HLA-DRB1*15. Our observations supported the epidemiologic link between MS and childhood ALL and added the sex effect to this connection. It appears that only girls born to mothers with MS may have an increased risk of ALL. Investigating the mechanism of these sex-specific associations may help understand the pathogenesis of MS and ALL. © 2010 Informa UK, Ltd.

Ucisik-Akkaya E.,HUMIGEN LLC | Davis C.F.,HUMIGEN LLC | Gorodezky C.,Institute Diagnostico y Referencia Epidemiologicos InDRE | Alaez C.,Institute Diagnostico y Referencia Epidemiologicos InDRE | Dorak M.T.,HUMIGEN LLC
Cell Stress and Chaperones | Year: 2010

Three heat shock protein 70 (HSP70) genes, HSPA1L, HSPA1A, and HSPA1B, are located within the human leukocyte antigen (HLA) class III region. HSPs act as stress signals and regulate natural killer cell response to cancer. HSP70 gene polymorphisms show disease associations partly due to their linkage disequilibrium with HLA alleles. To systematically evaluate their associations with childhood acute lymphoblastic leukemia (ALL), we examined the three functional single nucleotide polymorphisms (SNPs) rs2227956 (T493M) in HSPA1L, rs1043618 in HSPA1A 5′ UTR, and rs1061581 (Q351Q) in HSPA1B by TaqMan assays or polymerase chain reaction-restriction fragment length polymorphism in 114 ALL cases and 414 controls from Wales (UK), in 100 Mexican Mestizo ALL cases and 253 controls belonging to the same ethnic group, and in a panel of 82 HLA-typed reference cell line samples. Homozygosity for HSPA1B rs1061581 minor allele G was associated with protection (odds ratio (OR)∈=∈0.37, 95% confidence interval (CI)∈=∈0.16-0.78; P∈=∈0.007) with gene-dosage effect (additive model) reaching significance (P∈=∈0.0001) in the Welsh case-control group. This association was replicated in the second case-control group from Mexico (OR (recessive model)∈=∈0.49, 95% CI∈=∈0.24-0.96; P∈=∈0.03), and the pooled analysis yielded a strong association (Mantel-Haenszel OR∈=∈0.43, 95% CI∈=∈0.27-0.69, P∈=∈0.0004). The association was stronger in males in each group and in the pooled analysis. A three-SNP haplotype including the major allele A of rs1061581 showed a highly significant increase in Welsh cases compared with respective controls (6.7% vs 1.8%; P∈=∈0.0003) due to the difference between male cases and controls. The protective allele of rs1061581 occurred more frequently on the HLA-DRB3 haplotypes (especially DRB1 03) in the cell line panel, but the HSPA1B association was independent from the HLA-DRB4 association previously detected in the same case-control group from Wales (adjusted P∈=∈0.001). Given the cancer promoting roles played by HSPs intracellularly as well as roles in immune surveillance when expressed on the cell surface and the known correlations between expression levels and the HSP polymorphisms, these results are likely to indicate a primary association and warrant detailed assessment in childhood ALL development. © 2009 Cell Stress Society International.

Han X.Y.,University of Houston | Sizer K.C.,University of Houston | Velarde-Felix J.S.,Autonomous University of Sinaloa | Frias-Castro L.O.,Autonomous University of Sinaloa | Vargas-Ocampo F.,Institute Diagnostico y Referencia Epidemiologicos InDRE
International Journal of Dermatology | Year: 2012

Background Mycobacterium leprae was the only known cause of leprosy until 2008, when a new species, named Mycobacterium lepromatosis, was found to cause diffuse lepromatous leprosy (DLL), a unique form of leprosy endemic in Mexico. Methods We sought to differentiate the leprosy agents among 120 Mexican patients with various clinical forms of leprosy and to compare their relative prevalences and disease features. Archived skin biopsy specimens from these patients were tested for both M. leprae and M. lepromatosis using polymerase chain reaction-based species-specific assays. Results Etiologic species were confirmed in 87 (72.5%) patients, of whom 55 were infected with M. lepromatosis, 18 with M. leprae, and 14 with both organisms. The endemic regions of each agent differed but overlapped. Patients with M. lepromatosis were younger and were distributed across more states; their clinical diagnoses included DLL (n=13), lepromatous leprosy (LL) (n=34), and eight other forms of leprosy. By contrast, the diagnoses of patients with M. leprae did not include DLL but did include LL (n=15) and three other forms of leprosy. Thus, M. lepromatosis caused DLL specifically (P=0.023). Patients with M. lepromatosis also showed more variable skin lesions; the extremities were the most common sites of biopsy in these patients. Finally, patients with dual infections manifested all clinical forms and accounted for 16.1% of all species-confirmed cases. Conclusions Mycobacterium lepromatosis is another cause of leprosy and is probably more prevalent than M. leprae in Mexico. It mainly causes LL and also specifically DLL. Dual infections caused by both species may occur in endemic areas. © 2012 The International Society of Dermatology.

Huerta H.,Institute Diagnostico y Referencia Epidemiologicos InDRE | Haenni J.-P.,Museum dHistoire Naturelle
Zootaxa | Year: 2016

Four new species of the recently described genus Aztecatopse Haenni & Huerta are described from Mexico. Three of them, Aztecatopse amorimi sp. nov., A. foliosa sp. nov. and A. spinosa sp. nov. occur sympatrically with the type-species of the genus in central region (state of Mexico), while A. neotropica sp. nov. has been collected in states of Guerrero, Jalisco and Morelos. A key to the males of all known species of the genus is provided. Copyright © 2016 Magnolia Press.

PubMed | Institute Diagnostico y Referencia Epidemiologicos InDRE and Museum dhistoire naturelle Rue des Terreaux 14 CH 2000 Neuchatel Switzerland .; Email
Type: Journal Article | Journal: Zootaxa | Year: 2016

Four new species of the recently described genus Aztecatopse Haenni & Huerta are described from Mexico. Three of them, Aztecatopse amorimi sp. nov., A. foliosa sp. nov. and A. spinosa sp. nov. occur sympatrically with the type-species of the genus in central region (state of Mexico), while A. neotropica sp. nov. has been collected in states of Guerrero, Jalisco and Morelos. A key to the males of all known species of the genus is provided.

PubMed | National Autonomous University of Mexico and Institute Diagnostico y Referencia Epidemiologicos INDRE
Type: | Journal: Parasitology | Year: 2017

Chagas disease is one of the most important vector-borne zoonotic diseases in Latin America. Control strategies could be improved if transmissibility patterns of its aetiologic agent, Trypanosoma cruzi, were better understood. To understand transmissibility patterns of Chagas disease in Mexico, we inferred potential vectors and hosts of T. cruzi from geographic distributions of nine species of Triatominae and 396 wild mammal species, respectively. The most probable vectors and hosts of T. cruzi were represented in a Complex Inference Network, from which we formulated a predictive model and several associated hypotheses about the ecological epidemiology of Chagas disease. We compiled a list of confirmed mammal hosts to test our hypotheses. Our tests allowed us to predict the most important potential hosts of T. cruzi and to validate the model showing that the confirmed hosts were those predicted to be the most important hosts. We were also able to predict differences in the transmissibility of T. cruzi among triatomine species from spatial data. We hope our findings help drive efforts for future experimental studies.

PubMed | Institute Diagnostico y Referencia Epidemiologicos InDRE, National Polytechnic Institute of Mexico and Hospital Of Pediatria
Type: Case Reports | Journal: Archives of microbiology | Year: 2016

The genus Psychrobacter contains environmental, psychrophilic and halotolerant gram-negative bacteria considered rare opportunistic pathogens in humans. Metagenomics was performed on the cerebrospinal fluid (CSF) of a pediatric patient with meningitis. Nucleic acids were extracted, randomly amplified, and sequenced with the 454 GS FLX Titanium next-generation sequencing (NGS) system. Sequencing reads were assembled, and potential virulence genes were predicted. Phylogenomic and phylogenetic studies were performed. Psychrobacter sp. 310 was identified, and several virulence genes characteristic of pathogenic bacteria were found. The phylogenomic study and 16S rRNA gene phylogenetic analysis showed that the closest relative of Psychrobacter sp. 310 was Psychrobacter sanguinis. To our knowledge, this is the first report of a meningitis case associated with Psychrobacter sp. identified by NGS metagenomics in CSF from a pediatric patient. The metagenomic strategy based on NGS was a powerful tool to identify a rare unknown pathogen in a clinical case.

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