Cruz-Rivera M.,National Autonomous University of Mexico |
Carpio-Pedroza J.C.,Institute Diagnostico y Referencia Epidemiologicos |
Escobar-Gutierrez A.,Institute Diagnostico y Referencia Epidemiologicos |
Lozano D.,National Polytechnic Institute of Mexico |
And 6 more authors.
Journal of Clinical Microbiology | Year: 2013
Here, we analyze the viral divergence among hepatitis C virus (HCV) chronic cases infected with genotype 1. The intrahost viral evolution was assessed by deep sequencing using the 454 Genome Sequencer platform. The results showed a rapid nucleotide sequence divergence. This notorious short-term viral evolution is of the utmost importance for the study of HCV transmission, because direct links between related samples were virtually lost. Thus, rapid divergence of HCV significantly affects genetic relatedness studies and outbreak investigations. Copyright © 2013, American Society for Microbiology.
Interleukin-28B genotyping by melt-mismatch amplification mutation assay PCR analysis using single nucleotide polymorphisms rs12979860 and rs8099917, a useful tool for prediction of therapy response in hepatitis C patients
Fonseca-Coronado S.,National Autonomous University of Mexico |
Vaughan G.,Institute Diagnostico Y Referencia Epidemiologicos |
Cruz-Rivera M.Y.,National Autonomous University of Mexico |
Carpio-Pedroza J.C.,Institute Diagnostico Y Referencia Epidemiologicos |
And 3 more authors.
Journal of Clinical Microbiology | Year: 2011
Several studies have identified associations between single nucleotide polymorphisms (SNPs) occurring near the interleukin-28B (IL-28B) gene and response to antiviral treatment among hepatitis C virus (HCV) patients. Here, we describe a reliable melt-mismatch amplification mutation assay (melt-MAMA) PCR-based genotyping method for IL-28B which can be used in the management of HCV patients, helping to better define the course of therapy. Copyright © 2011, American Society for Microbiology. All Rights Reserved.
Sarmiento-Silva R.E.,National Autonomous University of Mexico |
Nakamura-Lopez Y.,Consejo Estatal para la Prevencion y Control del Sida Centro Ambulatorio para la Prevencion |
Vaughan G.,Institute Diagnostico y Referencia Epidemiologicos
Viruses | Year: 2012
The bovine respiratory syncytial virus (BRSV) is an enveloped, negative sense, single-stranded RNA virus belonging to the pneumovirus genus within the family Paramyxoviridae. BRSV has been recognized as a major cause of respiratory disease in young calves since the early 1970s. The analysis of BRSV infection was originally hampered by its characteristic lability and poor growth in vitro. However, the advent of numerous immunological and molecular methods has facilitated the study of BRSV enormously. The knowledge gained from these studies has also provided the opportunity to develop safe, stable, attenuated virus vaccine candidates. Nonetheless, many aspects of the epidemiology, molecular epidemiology and evolution of the virus are still not fully understood. The natural course of infection is rather complex and further complicates diagnosis, treatment and the implementation of preventive measures aimed to control the disease. Therefore, understanding the mechanisms by which BRSV is able to establish infection is needed to prevent viral and disease spread. This review discusses important information regarding the epidemiology and molecular epidemiology of BRSV worldwide, and it highlights the importance of viral evolution in virus transmission. © 2012 by the authors; licensee MDPI, Basel, Switzerland.
Rodriguez-Castillo A.,Institute Diagnostico y Referencia Epidemiologicos |
Vaughan G.,Institute Diagnostico y Referencia Epidemiologicos |
Ramirez-Gonzalez J.E.,Institute Diagnostico y Referencia Epidemiologicos |
Escobar-Gutierrez A.,Institute Diagnostico y Referencia Epidemiologicos
Journal of Clinical Microbiology | Year: 2010
Full-length genome analysis of varicella-zoster virus (VZV) has shown that viral strains can be classified into seven different genotypes: European (E), Mosaic (M), and Japanese (J), and the E and M genotypes can be further subclassified into E1, E2, and M1 through 4, respectively. The distribution of the main VZV genotypes in Mexico was described earlier, demonstrating the predominance of E genotype, although other genotypes (M1 and M4) were also identified. However, no information regarding the circulation of either E genotype in the country is available. In the present study, we confirm the presence of both E1 and E2 genotypes in the country and explore the possibility of coinfection as the triggering factor for increased virulence among severe cases. A total of 61 different European VZV isolates collected in the Mexico City metropolitan area from 2005 to 2006 were typed by using a PCR method based on genotype-specific primer amplification. Fifty isolates belonged to the E1 genotype, and the eleven remaining samples were classified as E2 genotypes. No coinfection with both E genotypes was identified among these specimens. We provide here new information on the distribution of VZV genotypes circulating in Mexico City. Copyright © 2010, American Society for Microbiology. All Rights Reserved.
Alaez C.,Institute Diagnostico y Referencia Epidemiologicos |
Alaez C.,Instituto Nacional Of Medicina Genomica |
Flores-A H.,Institute Diagnostico y Referencia Epidemiologicos |
Munguia A.,Institute Diagnostico y Referencia Epidemiologicos |
Gorodezky C.,Institute Diagnostico y Referencia Epidemiologicos
Tissue Antigens | Year: 2015
The B*14:41N allele was identified in a The National Marrow Donor Program (NMDP) Hispanic donor typed by our Mexican Registry-DONORMO © 2015 John Wiley & Sons A/S.
De La Cruz-Hernandez S.I.,Institute Diagnostico Y Referencia Epidemiologicos |
Flores-Aguilar H.,Institute Diagnostico Y Referencia Epidemiologicos |
Gonzalez-Mateos S.,Institute Diagnostico Y Referencia Epidemiologicos |
Lopez-Martinez I.,Institute Diagnostico Y Referencia Epidemiologicos |
And 3 more authors.
American Journal of Tropical Medicine and Hygiene | Year: 2013
Higher levels of viremia and circulating nonstructural protein 1 (NS1) have been associated with dengue disease severity. In this study, viremia and circulating NS1 levels were determined in 225 serum samples collected from patients in Mexico infected with dengue virus serotypes 1 and 2 (DENV-1 and DENV-2). Patients with dengue hemorrhagic fever (DHF) who were infected with DENV-1 showed higher levels of circulating NS1 than patients with dengue fever (DF) (P = 0.0175). Moreover, NS1 levels were higher in patients with primary infections with DENV-1 than in patient infected with DENV-2 (P < 0.0001) and in patients with primary infections with DENV-2 than in patients with secondary infections with DENV-2 (P = 0.0051). Unexpectedly, viremia levels were higher in patients with DF than in those with DHF infected with either DENV-1 or DENV-2 (P = 0.0019 and P = 0.001, respectively) and in patients with primary infections than those with secondary DENV-2 infections (P < 0.0001). Results indicate that levels of circulating NS1 vary according to the infecting serotype, immunologic status (primary or secondary infection), and dengue disease severity. Copyright © 2013 by The American Society of Tropical Medicine and Hygiene.
Meza-Marquez O.G.,Escuela Nacional de Ciencias Biologicas |
Gallardo-Velazquez T.,Escuela Nacional de Ciencias Biologicas |
Dorantes-Alvarez L.,Escuela Nacional de Ciencias Biologicas |
Osorio-Revilla G.,Escuela Nacional de Ciencias Biologicas |
De La Rosa Arana J.L.,Institute Diagnostico y Referencia Epidemiologicos
Analyst | Year: 2011
A fast and simple screening method for the determination of clenbuterol at the ppb level in a murine model was demonstrated by Mid Infrared (MIR) and Raman spectroscopy in conjunction with multivariate analysis. In order to build the calibration models to quantify clenbuterol in rat meat, mixtures of rat meat and clenbuterol were prepared in a range of 5-10000 ppb. Partial Least Square (PLS) analysis was used to build the calibration model. The results shown that Mid Infrared and Raman spectroscopy were efficient, but Mid Infrared (R 2 = 0.966 and SEC = 0.27) were superior to Raman (R 2 = 0.914 and SEC = 1.167). The SIMCA model developed showed 100% classification rate of rat meat samples with or without clenbuterol. The results were confirmed with contaminated meat samples from animals treated with clenbuterol. Chemometric models represent an attractive option for meat quality screening without sample pretreatments which can identify veterinary medicinal products at the ppb level. © 2011 The Royal Society of Chemistry.
Gonzalez-Gomez B.E.,Institute Diagnostico y Referencia Epidemiologicos
Journal of the Egyptian Society of Parasitology | Year: 2012
Human onchocerciasis is a disease that remains as an important public health problem. The morphometric and physical characteristics of 363 Onchocerca volvulus nodules collected in the major endemic focus of onchocerciasis in Southern Chiapas (Soconusco), was assessed. In the present work we found that treatment the morphometry of 363 onchocercal nodules preserved in a 67% glycerol solution was determined by measuring the length, width and thick of each nodule with a Vernier caliper. The mass was determined with an analytical balance and the volume by measuring the water displacement, while the specific gravity was calculated by dividing mass over the volume. Statistical analysis was calculated for each parameter. The results showed that the nodules were rather longer than wider or thicker. Morphometric characteristics were 9.87 +/-3.70 (mean +/- standard deviation), 7.52 +/- 2.81, and 4.62 +/-+/- 2.06 mm for length, width and thick respectively. In regard to the shape, 62.81% of the nodules showed a lenticular shape, while 18.18% were spherical and 19.01% were ovoid. Based on the distribution of frequencies of the length, the nodules were classified in three groups: the "small" (5.77 +/- 0.73 mm; n = 104, 28.65%), the "medium" group (9.86 +/- 2.05 mm; n = 203 nodules, 55.92%), and the group of the "big" ones (16.03 +/- 1.91 mm; n = 56, 15.43%). Moreover, the physical characteristics were: for the mass 0.33 +/- 0.24 g, the volume of displaced water was 0.28 +/- 0.26 ml, and the specific gravity was 1.10 +/- 0.55 g/ml. The results indicated that most of the Mexican Onchocerca nodules have a lenticular shape with average size of 10x7x5 mm, which is useful in the knowledge of the genus biodiversity and can be taken as a parameter in clinical or epidemiological trials, where onchocerciasis remains as a public health problem.
Sanchez Perez H.J.,Colegio de Mexico |
Diaz-Vazquez A.,Colegio de Mexico |
Najera-Ortiz J.C.,Colegio de Mexico |
Balandrano S.,Institute Diagnostico Y Referencia Epidemiologicos |
Martin-Mateo M.,Autonomous University of Barcelona
International Journal of Tuberculosis and Lung Disease | Year: 2010
OBJECTIVES: To analyse the proportion of multidrug-resistant tuberculosis (MDR-TB) in cultures performed during the period 2000-2002 in Los Altos, Selva and Norte regions, Chiapas, Mexico, and to analyse MDR-TB in terms of clinical and sociodemographic indicators. METHODS: Cross-sectional study of patients with pulmonary tuberculosis (PTB) from the above regions. Drug susceptibility testing results from two research projects were analysed, as were those of routine sputum samples sent in by health personnel for processing (n = 114). MDR-TB was analysed in terms of the various variables of interest using bivariate tests of association and logistic regression. RESULTS: The proportion of primary MDR-TB was 4.6% (2 of 43), that of secondary MDR-TB was 29.2% (7/24), while among those whose history of treatment was unknown the proportion was 14.3% (3/21). According to the logistic regression model, the variables most highly associated with MDR-TB were as follows: having received anti-tuberculosis treatment previously, cough of >3 years' duration and not being indigenous. CONCLUSIONS: The high proportion of MDR cases found in the regions studied shows that it is necessary to significantly improve the control and surveillance of PTB. © 2010 The Union.
Goncalves Rossi L.M.,São Paulo State University |
Escobar-Gutierrez A.,Institute Diagnostico y Referencia Epidemiologicos |
Rahal P.,São Paulo State University
Viruses | Year: 2015
Hepatitis C virus (HCV) infection is an important public health problem worldwide. HCV exploits complex molecular mechanisms, which result in a high degree of intrahost genetic heterogeneity. This high degree of variability represents a challenge for the accurate establishment of genetic relatedness between cases and complicates the identification of sources of infection. Tracking HCV infections is crucial for the elucidation of routes of transmission in a variety of settings. Therefore, implementation of HCV advanced molecular surveillance (AMS) is essential for disease control. Accounting for virulence is also important for HCV AMS and both viral and host factors contribute to the disease outcome. Therefore, HCV AMS requires the incorporation of host factors as an integral component of the algorithms used to monitor disease occurrence. Importantly, implementation of comprehensive global databases and data mining are also needed for the proper study of the mechanisms responsible for HCV transmission. Here, we review molecular aspects associated with HCV transmission, as well as the most recent technological advances used for virus and host characterization. Additionally, the cornerstone discoveries that have defined the pathway for viral characterization are presented and the importance of implementing advanced HCV molecular surveillance is highlighted. © 2015 by the authors; licensee MDPI, Basel, Switzerland.