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Barbabosa-Pliego A.,University of Central Mexico | Gil P.C.,University of Central Mexico | Hernandez D.O.,University of Central Mexico | Aparicio-Burgos J.E.,University of Central Mexico | And 8 more authors.
Vector-Borne and Zoonotic Diseases | Year: 2011

American trypanosomiasis is a public health problem in Latin America and southern parts of the United States. Infection in triatomines (vector) and domestic dogs (reservoir host) is a good indicator of Trypanosoma cruzi circulation and human risk of infection. The State of Mexico, Mexico, has been considered free of T. cruzi, and no detailed epidemiologic study has been conducted to assess the intricacies of the transmission cycle of the parasite in the region. Such studies would enhance our understanding of the epidemiology of T. cruzi infection in this geographic region and provide regional sanitary authorities with stronger fundamental knowledge for making decisions and allocating funds for Chagas disease control programs in the State of Mexico. The objective of this study was to determine the prevalence of T. cruzi infection in dogs (seroprevalence) and triatomines (fecal parasites) in a previously identified, discrete endemic region of parasite circulation and to widen our studies in the Tejupilco Sanitary Region located in the southern part of the State of Mexico. Dog blood samples (n = 102) were analyzed for the presence of anti-T. cruzi antibodies by two assays, namely indirect hemagglutination assay and enzyme-linked immunosorbent assay. Triatomines (n = 88) were collected and fecal aliquots were analyzed for the presence of parasites by light microscopy. Average seroprevalence in dogs in the Tejupilco Sanitary region was 24.5%, and the overall triatomine infection rate was 34.01%. Triatoma pallidipennis was the only triatomine species found in this region. Our data demonstrate that T. cruzi is actively circulating in the Tejupilco Sanitary Region and emphasize the requirement for epidemiologic surveillance programs throughout the putative endemic areas of the State of Mexico. © Copyright 2011, Mary Ann Liebert, Inc. 2011. Source


Alaez C.,Institute Diagnostico y Referencia Epidemiologicas | Flores-A H.,Institute Diagnostico y Referencia Epidemiologicas | Concha del Rio L.E.,Inflammatory Eye Disease Clinic | Munguia A.,Institute Diagnostico y Referencia Epidemiologicas | And 4 more authors.
Human Immunology | Year: 2011

Vogt-Koyanagi-Harada syndrome (VKH) is a multisystem autoimmune disorder mediated by cytotoxic T cells targeting melanocytes antigen(s). A strong major histocompatibility complex (MHC) association with HLA-DRB1*04:05 has been demonstrated in different populations. We investigated the contribution of HLA-A*, -B*, -C*, -DRB1*, and -DQB1* genes, belonging to the human leukocyte antigen (HLA), to the expression of VKH and we analyzed the influence of gender on the HLA association. A total of 76 patients and 256 healthy Mexican Mestizo individuals were included. HLA-A, B, C, and DQB1 typing was performed using the polymerase chain reaction, and hybridization was done using sequence specific probes. DRB1 alleles were defined by means of sequence base typing. The frequency of DRB1*04:05 (odds ratio = 2.95) and DRB1*04:04 (odds ratio = 2.79) were found to be significantly increased in the patients, conferring a similar risk. Gender stratification analysis showed that these alleles were associated with female gender only. No HLA class I or class II alleles were significantly deviated in males. The frequency of DRB1*04:07 was increased in the whole group, upon withdrawal from analysis the DRB1*04:04 and *04:05 positive patients. A trend of DRB1 alleles contributing to the expression of VKH is suggested: DRB1*04:05 = *04:04 > *04:07 > *01:01 > *01:02. Although none of the results were significant after the p value was corrected, the data are consistent with those in numerous other studies, suggesting that several different DRB1* alleles may be involved in the etiopathogenesis of the disease by presenting an overlapping set of ocular peptides to the T cells, which in turn may trigger the autoimmune response that is present in the patients. © 2011. Source


Alaez C.,Institute Diagnostico y Referencia Epidemiologicas | Flores-A H.,Institute Diagnostico y Referencia Epidemiologicas | Munguia A.,Institute Diagnostico y Referencia Epidemiologicas | Valencia M.,Institute Diagnostico y Referencia Epidemiologicas | Gorodezky C.,Institute Diagnostico y Referencia Epidemiologicas
Tissue Antigens | Year: 2013

HLA-B*35:233 a novel human leukocyte antigen-B was identified in a Mexican Mestizo donor. © 2013 John Wiley & Sons A/S. Source

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