Geissler M.,Institute des materiaux industriels IMI |
Beauregard J.A.,Institute des materiaux industriels IMI |
Beauregard J.A.,Laval University |
Charlebois I.,Laval University |
And 6 more authors.
Engineering in Life Sciences | Year: 2011
This paper describes an experimentally simple and efficient way of integrating bead-based mechanical cell lysis on a plastic chip. The chip is fabricated from machined slides of poly(methylmethacrylate) and accommodates a metal disk along with solid microbeads in a designated lysis chamber. Magnetic actuation of the metal disk induces collisions and frictional forces within the lysis matrix causing cell disruption. The efficiency of nucleic acid extraction was investigated using spores of Bacillus atrophaeus subsp. globigii and the amount of genomic DNA in the lysates has been quantified by real-time PCR. Compared to a standardized DNA extraction method based on BD GeneOhm™ Lysis Kit, the yield was dependent on the composition of the lysis matrix, including size and relative amount of microbeads, along with instrumental parameters, such as duration and frequency of agitation. The interplay of these parameters should allow for optimizing lysis protocols to faithfully disrupt any particular type of cell without affecting the genetic material to be extracted. © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Oteyaka M.O.,University of Québec |
Oteyaka M.O.,Laval University |
Chevallier P.,University of Québec |
Chevallier P.,Laval University |
And 5 more authors.
Plasma Chemistry and Plasma Processing | Year: 2012
We investigated the effect of a polyethylene terephthalate (PET) forming process on radiofrequency ammonia plasma surface-treated PET flat films and fibres obtained by melt blowing. Ammonia plasma treatment allowed for the incorporation of amino functionalities on both the film and fibre surfaces, with higher values observed at very short treatment times. This plasma treatment also induced polymer chain scissions which were observed as the formation of hydrophilic nodules that coalesced together and were loosely bound to the underlying polymeric materials. These plasma-induced surface damages were notably more important on the melt-blown PET fibres. Consequently, maximisation of the surface amino groups with minimal polymer chain breaking was achieved using very short plasma treatment times (typically 1 s). We also demonstrated that the polymer forming process must be taken into account when plasma modifications are to be performed on PET, as it may already lead to polymer chain breakings subsequently added to those induced in the plasma environment. © Springer Science+Business Media, LLC 2011.