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Rio de Janeiro, Brazil

Napoleao K.S.,National Institute of Metrology of Brazil | Napoleao K.S.,Federal University of Rio de Janeiro | Mello-Patiu C.A.,Federal University of Rio de Janeiro | Oliveira-Costa J.,Institute Criminalistica Carlos Eboli | And 4 more authors.
Genetics and Molecular Research | Year: 2016

Sarcophagidae, or flesh flies, are of great importance in forensic entomology, but their effective application requires precise taxonomic identification, which relies almost exclusively on characteristics of the male genitalia. Given that female flies and larvae are most abundant in animal carcasses or on corpses, precise morphological identification can be difficult; therefore, DNA sequencing can be an additional tool for use in taxonomic identification. This paper analyzes part of the mitochondrial cytochrome c oxidase subunit I (COI) gene from three Sarcophagidae species of forensic importance in the City of Rio de Janeiro: Oxysarcodexia fluminensis, Peckia chrysostoma, and Peckia intermutans. COI fragments of 400 bp from 36 specimens of these three species were sequenced. No intraspecific differences were found among specimens of O. fluminensis, but P. chrysostoma and P. intermutans each had two haplotypes, ranging from 0 to 0.7%. The interspecific divergence was 8.5-11.6%, corroborating previously reported findings. © UNPEC-RP. Source

Oliveira-Costa J.,Institute Criminalistica Carlos Eboli | Oliveira-Costa J.,Castelo Branco University | Lamego C.M.D.,Castelo Branco University | Couri M.S.,Federal University of Rio de Janeiro | And 3 more authors.
Brazilian Journal of Biology | Year: 2014

In the present contribution we compared the entomological succession pattern of a burned carcass with that of an unburned one. For that, we used domestic pig carcasses and focused on Calliphoridae, Muscidae and Sarcophagidae flies, because they are the ones most commonly used in Postmortem Interval estimates. Adult and immature flies were collected daily. A total of 27 species and 2,498 specimens were collected, 1,295 specimens of 26 species from the partially burned carcass and 1,203 specimens of 22 species from the control carcass (unburned). The species composition in the two samples differed, and the results of the similarity measures were 0.875 by Sorensen and 0.756 by Bray-Curtis index. The results obtained for both carcasses also differ with respect to the decomposition process, indicating that the post mortem interval would be underestimated if the entomological succession pattern observed for a carcass under normal conditions was applied to a carbonized carcass © 2014, Instituto Internacional de Ecologia. All rights reserved. Source

Romao W.,University of Campinas | Schwab N.V.,University of Campinas | Bueno M.I.M.S.,University of Campinas | Sparrapan R.,University of Campinas | And 5 more authors.
Quimica Nova | Year: 2011

In this review recent methods developed and applied to solve criminal occurences related to documentoscopy, ballistic and drugs of abuse are discussed. In documentoscopy, aging of ink writings, the sequence of line crossings and counterfeiting of documents are aspects to be solved with reproducible, fast and non-destructive methods. In ballistic, the industries are currently producing "lead-free" or "nontoxic" handgun ammunitions, so new methods of gunshot residues characterization are being presented. For drugs analysis, easy ambient sonic-spray ionization mass spectrometry (EASI-MS) is shown to provide a relatively simple and selective screening tool to distinguish m-CPP and amphetamines (MDMA) tablets, cocaine and LSD. Source

Dias G.S.,Federal University of Rio de Janeiro | Dias G.S.,Institute Criminalistica Carlos Eboli | Oliveira-Costa J.,Institute Criminalistica Carlos Eboli | de Mello-Patiu C.A.,Federal University of Rio de Janeiro
Revista Brasileira de Entomologia | Year: 2015

Sarcophagidae species are frequent and abundant in the decomposition process of corpses and, consequently, play an important role as a tool for the application of Forensic Entomology. Helicobia pilifera Lopes, 1939, Microcerella erythropyga (Lopes, 1936), Oxysarcodexia fringidea Curran & Walley, 1934 and Peckia (Peckia) pexata (Wulp, 1895) were recorded for the first time in a Forensic Entomology experiment in Rio de Janeiro, using domestic pig carcasses as substrate. © 2015 Sociedade Brasileira de Entomologia. Source

Porto S.K.S.S.,University of Sao Paulo | Porto S.K.S.S.,Institute Criminalistica Carlos Eboli | Nogueira T.,University of Sao Paulo | Blanes L.,University of Technology, Sydney | And 4 more authors.
Journal of Forensic Sciences | Year: 2014

A method for the identification of 3,4-methylenedioxymethamphetamine (MDMA) and meta-chlorophenylpiperazine (mCPP) was developed employing capillary electrophoresis (CE) with capacitively coupled contactless conductivity detection (C4D). Sample extraction, separation, and detection of "Ecstasy" tablets were performed in <10 min without sample derivatization. The separation electrolyte was 20 mm TAPS/Lithium, pH 8.7. Average minimal detectable amounts for MDMA and mCPP were 0.04 mg/tablet, several orders of magnitude lower than the minimum amount encountered in a tablet. Seven different Ecstasy tablets seized in Rio de Janeiro, Brazil, were analyzed by CE-C4D and compared against routine gas chromatography-mass spectrometry (GC-MS). The CE method demonstrated sufficient selectivity to discriminate the two target drugs, MDMA and mCPP, from the other drugs present in seizures, namely amphepramone, fenproporex, caffeine, lidocaine, and cocaine. Separation was performed in <90 sec. The advantages of using C4D instead of traditional CE-UV methods for in-field analysis are also discussed. © 2014 American Academy of Forensic Sciences. Source

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