Institute Criminalistica

Manaus, Brazil

Institute Criminalistica

Manaus, Brazil

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Alves H.B.,Institute Criminalistica | Alves H.B.,Pontifical Catholic University of Parana | Leite F.P.N.,Instituto Geral Of Pericias | Sotomaior V.S.,Pontifical Catholic University of Parana | And 4 more authors.
International Journal of Legal Medicine | Year: 2014

Allelic frequencies for 15 STR autosomal loci, using AmpFℓSTR® Identifiler™, forensic, and statistical parameters were calculated. All loci reached the Hardy-Weinberg equilibrium. The combined power of discrimination and mean power of exclusion were 0.999999999999999999 and 0.9999993, respectively. The MDS plot and NJ tree analysis, generated by FST matrix, corroborated the notion of the origins of the Paraná population as mainly European-derived. The combination of these 15 STR loci represents a powerful strategy for individual identification and parentage analyses for the Paraná population. © 2013 Springer-Verlag Berlin Heidelberg.


Cordeiro D.,National Institute of Amazonian Research | Bravo F.,State University of Feira de Santana | Chagas C.,Institute Criminalistica
Acta Entomologica Musei Nationalis Pragae | Year: 2015

The collection of psychodids of the Brazilian semiarid region, deposited at the Museu de Zoologia da Universidade Estadual de Feira de Santana (MZFS), was revisited, and four new species of Psychodinae are herein described: Feuerborniella jezeki Cordeiro & Bravo sp. nov., Feuerborniella concava Cordeiro & Bravo sp. nov. and Alepia janjezeki Cordeiro & Bravo sp. nov., from the state of Bahia, and Feuerborniella pilosella Cordeiro & Bravo sp. nov., from the state of Paraíba. The morphology of the new species of Trichopsychodina raised questions about the supraspecifi c classifi cation on this subtribe. These questions are discussed, concerning mainly the Neotropical fauna, and Feuerborniella Vaillant, 1974 is redefi ned, including species of Bahisepedon Omelková & Ježek, 2012. Based on this synonymy the following new combinations are made: Feuerborniella amblytes (Quate, 1999) comb. nov., F. ancepitis (Quate, 1996) comb. nov., F. bicuspis (Quate, 1996) comb. nov., F. hamata (Quate, 1996) comb. nov., F. oblongola (Bravo, Chagas & Cordeiro, 2006) comb. nov., F. opposita (Banks, 1901) comb. nov., F. pandiculata (Quate, 1996) comb. nov., F. retusa (Quate, 1996) comb. nov., F. uncinata (Bravo, Chagas & Cordeiro, 2006) comb. nov., and F. vieirai (Chagas, Bravo & Rafael, 2009) comb. nov. Moreover, one species known so far only from Costa Rica, Feuerborniella pollicaris (Quate, 1996) comb. nov., is transferred from Philosepedon Eaton, 1904, being the fi rst record from Brazil, in semiarid areas of the states of Bahia and Ceará. © 2015, National Museum/Narodni muzeum. All rights reserved.


Neiss U.G.,Institute Criminalistica | Hamada N.,National Institute of Amazonian Research
Zootaxa | Year: 2016

The larva of Palaemnema brasiliensis Machado, 2009 is described and illustrated based on last-instar larvae and exuviae of reared larvae collected in a blackwater stream in Barcelos and Presidente Figueiredo municipalities, Amazonas state, Brazil. The larva of P. brasiliensis can be distinguished from the two South American species of the genus with described larvae (P. clementia Selys and P. mutans Calvert), mainly by presence of a single obtuse cusp on the labial palp, the pres-ence and configuration of setae in the caudal lamellae, and the proportional length of terminal filaments of the caudal la-mellae. The family is recorded here for the first time in Brazilian state of Amazonas. Copyright © 2016 Magnolia Press.


PubMed | Institute Criminalistica and National Institute of Amazonian Research
Type: Journal Article | Journal: Zootaxa | Year: 2016

The larva of Palaemnema brasiliensis Machado, 2009 is described and illustrated based on last-instar larvae and exuviae of reared larvae collected in a blackwater stream in Barcelos and Presidente Figueiredo municipalities, Amazonas state, Brazil. The larva of P. brasiliensis can be distinguished from the two South American species of the genus with described larvae (P. clementia Selys and P. mutans Calvert), mainly by presence of a single obtuse cusp on the labial palp, the presence and configuration of setae in the caudal lamellae, and the proportional length of terminal filaments of the caudal lamellae. The family is recorded here for the first time in Brazilian state of Amazonas.


Kimura L.,University of Sao Paulo | Ribeiro-Rodrigues E.M.,Federal University of Pará | Ribeiro-Rodrigues E.M.,Institute Criminalistica | De Mello Auricchio M.T.B.,University of Sao Paulo | And 3 more authors.
American Journal of Human Biology | Year: 2013

Objectives: xMany Africans were brought to Brazil as slaves. The runaway or abandoned slaves founded isolated communities named quilombos. There are many quilombo remnants in Vale do Ribeira region in the southern part of São Paulo State. The aim of our study was to contribute to understanding the origins of these populations, through admixture studies. Methods: We genotyped 307 unrelated DNA samples obtained from ten quilombo populations from Vale do Ribeira region, using a panel of 48 INDEL polymorphisms. We estimated genetic differentiation between populations (FST) and genomic ancestry from these populations. Our data were compared to a similar study performed in quilombo remnants from the Brazilian Amazon region. Results: Population admixture estimates showed high degree of miscegenation in the quilombo remnants from Vale do Ribeira (average admixture estimates at 39.7% of African, 39.0% of European and 21.3% of Amerindian contribution). The proportions of ancestral genes varied greatly among individuals, ranging from 7.3 to 69.5%, 12.9 to 68.3%, and 7.3 to 58.5% (African, European, and Amerindian, respectively). Genetic differentiation between these populations was low (all FST values <5%), indicating gene flow between them. Both groups of quilombos, from Vale do Ribeira and Amazon, presented similar patterns of admixture. Conclusions: INDEL markers were useful to evidence the triple interbreeding among African, European, and Amerindian in the formation of quilombo populations. The low FST values suggested gene flow among quilombos from Vale do Ribeira. Our data highlight the important role of Amerindians in the formation of quilombo populations. © 2012 Wiley Periodicals, Inc.


Lanaro R.,University of Campinas | Costa J.L.,Institute Criminalistica | Zanolli Filho L.A.,University of Sao Paulo | Santos Cazenave S.O.,Nucleo de Pericias Criminalisticas de Campinas
Quimica Nova | Year: 2010

Designer drug is a term used to describe psychoactive drugs of abuse which are usually synthesized by modifying the molecular structures of existing drugs of abuse. The term gained widespread popularity when MDMA (ecstasy) experienced a popularity boom in the mid 1980's. In Brazil, designer drugs seizures have increased in the last few years, and actually tablets with unknown psychoactive compounds began to be forwarded to the Forensic Laboratories. This work describes the analytical assays that were performed to identify the chlorophenylpiperazine, a psychoactive substance first time identified in seized tablets in Sao Paulo state.


Floriani G.,Federal University of Paraná | Floriani G.,Institute Criminalistica | Gasparetto J.C.,Federal University of Paraná | Pontarolo R.,Federal University of Paraná | Goncalves A.G.,Federal University of Paraná
Forensic Science International | Year: 2014

Here, an HPLC-DAD method was developed and validated for simultaneous determination of cocaine, two cocaine degradation products (benzoylecgonine and benzoic acid), and the main adulterants found in products based on cocaine (caffeine, lidocaine, phenacetin, benzocaine and diltiazem). The new method was developed and validated using an XBridge C18 4.6. mm. ×. 250. mm, 5. μm particle size column maintained at 60. °C. The mobile phase consisted of a gradient of acetonitrile and ammonium formate 0.05. M - pH 3.1, eluted at 1.0. mL/min. The volume of injection was 10. μL and the DAD detector was set at 274. nm. Method validation assays demonstrated suitable sensitivity, selectivity, linearity, precision and accuracy. For selectivity assay, a MS detection system could be directly adapted to the method without the need of any change in the chromatographic conditions. The robustness study indicated that the flow rate, temperature and pH of the mobile phase are critical parameters and should not be changed considering the conditions herein determined. The new method was then successfully applied for determining cocaine, benzoylecgonine, benzoic acid, caffeine, lidocaine, phenacetin, benzocaine and diltiazem in 115 samples, seized in Brazil (2007-2012), which consisted of cocaine paste, cocaine base and salt cocaine samples. This study revealed cocaine contents that ranged from undetectable to 97.2%, with 97 samples presenting at least one of the degradation products or adulterants here evaluated. All of the studied degradation products and adulterants were observed among the seized samples, justifying the application of the method, which can be used as a screening and quantification tool in forensic analysis. © 2013 Elsevier Ireland Ltd.


Verde E.L.,Federal University of Goais | Verde E.L.,Federal University of Mato Grosso | Landi G.T.,University of Sao Paulo | Carriao M.S.,Federal University of Goais | And 5 more authors.
AIP Advances | Year: 2012

Further advances in magnetic hyperthermia might be limited by biological constraints, such as using sufficiently low frequencies and low field amplitudes to inhibit harmful eddy currents inside the patient's body. These incite the need to optimize the heating efficiency of the nanoparticles, referred to as the specific absorption rate (SAR). Among the several properties currently under research, one of particular importance is the transition from the linear to the non-linear regime that takes place as the field amplitude is increased, an aspect where the magnetic anisotropy is expected to play a fundamental role. In this paper we investigate the heating properties of cobalt ferrite and maghemite nanoparticles under the influence of a 500 kHz sinusoidal magnetic field with varying amplitude, up to 134 Oe. The particles were characterized by TEM, XRD, FMR and VSM, from which most relevant morphological, structural and magnetic properties were inferred. Both materials have similar size distributions and saturation magnetization, but strikingly different magnetic anisotropies. From magnetic hyperthermia experiments we found that, while at low fields maghemite is the best nanomaterial for hyperthermia applications, above a critical field, close to the transition from the linear to the non-linear regime, cobalt ferrite becomes more efficient. The results were also analyzed with respect to the energy conversion efficiency and compared with dynamic hysteresis simulations. Additional analysis with nickel, zinc and copper-ferrite nanoparticles of similar sizes confirmed the importance of themagnetic anisotropy and the damping factor. Further, the analysis of the characterization parameters suggested core-shell nanostructures, probably due to a surface passivation process during the nanoparticle synthesis. Finally, we discussed the effect of particle-particle interactions and its consequences, in particular regarding discrepancies between estimated parameters and expected theoretical predictions. Copyright 2012 Author(s).


PubMed | Institute Criminalistica, Catholic University of Brasília, University of Sao Paulo and University of Brasilia
Type: Case Reports | Journal: Journal of forensic sciences | Year: 2015

Little is known regarding the scavenger fauna associated with buried human corpses, particularly in clandestine burials. We report the presence of 20 shells of the terrestrial snail Allopeas micra, within hollow bones of human remains buried for 5years, during the process of collecting DNA material. The fact that a large number of shells of A.micra had been found in the corpse and in the crime scene supports the assumption that there was no attempt to remove the corpse from the area where the crime occurred. Despite this, our observations cannot be used to estimate the postmortem interval because there is no precise knowledge about the development of this species. This is the first record of a terrestrial snail associated with a human corpse and its role in this forensic medicine case.


Ribeiro-Rodrigues E.M.,Federal University of Pará | Ribeiro-Rodrigues E.M.,Institute Criminalistica | Palha T.D.J.B.F.,Federal University of Pará | Palha T.D.J.B.F.,Institute Criminalistica | And 3 more authors.
International Journal of Legal Medicine | Year: 2011

The admixed Brazilian population shows high levels of genetic variability, which resulted from the contribution of three main ethnicities, Amerindian, European, and African. However, due to its huge territory, admixing has been asymmetrical, i.e., the relative contribution from each ethnicity has been unequal in the five geopolitical regions of the country. The aim of this study was to describe genetic variability using a panel of short-tandem repeats on the X chromosome (X-STR) in order to perform a comprehensive evaluation of the usefulness of such markers for forensic purposes in Brazil. Twelve X-STR (DXS9895, DXS7132, DXS6800, DXS9898, DXS6789, DXS7133, GATA172D05, DXS7130, HPRTB, GATA31E08, DXS7423, and DXS10011) were chosen and tested in a sample of 2,234 individuals belonging to 16 out of the 27 Brazilian States, representing all of its five geopolitical regions. No markers showed significant deviation from the Hardy-Weinberg equilibrium, even when analyses were partitioned to represent geopolitical regions. Genetic diversity per locus ranged from 67% (DSX7133) to 95% (DXS10011), and the State of Ceará showed the highest average genetic diversity (79% for all 12 X-STR markers). Considering the Brazilian population as a whole, the power of discrimination of the 12 X-STR panel in females (PDF) was 0.999999999999994, while the power of discrimination in males (PDM) was 0.9999999969. Such high values suggest the potential of that panel to be used in forensic applications and relatedness tests among individuals. Comparisons among the Brazilian populations investigated revealed significant differences when they were compared among each other, a pattern that was maintained when additional populations from Europe and Latin America were compared to Brazilians. Our results highlight the need and usefulness of specific genetic database for forensic purposes in Brazilian populations. © 2011 Springer-Verlag.

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