Institute Ciencias Marinas Of Andalucia Icman

Puerto Real, Spain

Institute Ciencias Marinas Of Andalucia Icman

Puerto Real, Spain
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Pujante I.M.,University of Cádiz | Martos-Sitcha J.A.,Institute Ciencias Marinas Of Andalucia Icman | Moyano F.J.,University of Almeria | Ruiz-Jarabo I.,University of Cádiz | And 2 more authors.
Comparative Biochemistry and Physiology Part - B: Biochemistry and Molecular Biology | Year: 2015

The effects of starvation and re-feeding on metabolites and tissue composition, GH/IGF-I axis, and digestive enzyme activities in juvenile thick-lipped grey mullet (Chelon labrosus) were evaluated. Fish were divided into three feeding groups (n = 72, 82.00 ± 4.09. g initial body mass). The control group was fed 1% of their body mass once a day throughout the experiment with commercial pellets. The other two groups were deprived of feed for 21. days (starved), or re-fed for 7. days after 14. days of food deprivation (re-fed). Full-length cDNAs from pituitary GH and hepatic IGF-I were cloned by screening a cDNA library or by PCR techniques. Furthermore, changes in their mRNA expressions were assessed by real time PCR in specimens maintained under the different feeding patterns. Results showed a negative growth in starved and re-feeding groups. Starvation induced a significant increase in plasma triglycerides as well as a decrease in liver glucose and glycogen. Re-feeding increased plasma glucose, lactate and protein, as well as liver glucose and glycogen. In addition, starvation significantly increased pituitary GH expression, while re-feeding down-regulated it. No significant changes were observed in hepatic IGF-I expression in any dietary treatment. Digestive enzyme activities were not significantly affected either by starvation or by re-feeding. The results of the present work suggest that juveniles of the thick-lipped grey mullet may easily adjust their metabolism under situations characterized by a short-term starvation and re-feeding. © 2014 Elsevier Inc.


Perera E.,University of Habana | Perera E.,Institute Ciencias Marinas Of Andalucia Icman | Simon C.,University of Tasmania
Reviews in Aquaculture | Year: 2015

The development of cost-effective and nutritionally adequate formulated feeds is a key step for developing sustainable technologies for new aquaculture species. There has been many research effort for over 30 years on feed development for spiny lobsters, but amazingly, poor performance of formulated feeds remains one of the major obstacles to progressing commercial aquaculture of these crustaceans. This is partially due to a lack of information on how spiny lobsters digest and assimilate formulated feeds. The aim of this review is to integrate recent information on the digestive physiology of spiny lobsters to identify areas where further studies are needed for advancing to more physiologically tailored formulated feeds. Increasing the efficiency of mechanical and chemical digestion is imperative for better digestion of formulated feeds. This is likely to be achieved by improving feed format, reducing the particle size of ingredients, using digestible binders, preprocessing or selecting more soluble macronutrients and supplementing feeds with additives such as pH buffers, emulsifying agents and/or exogenous enzymes. Future research needs to adopt a holistic approach for investigating the digestive processes in spiny lobsters by focusing on digestion as well as downstream processes. The protein-sparing effect of lipids and carbohydrates on spiny lobster metabolism is of a particular significance as the spiny lobster metabolism is strongly directed towards the use of protein. This review provides important insights, practical solutions and key research directions to improve both our understanding of spiny lobster digestive physiology and the performance of formulated feeds for spiny lobsters. © 2015 Wiley Publishing Asia Pty Ltd.


Gilannejad N.,Isfahan University of Technology | Gilannejad N.,Institute Ciencias Marinas Of Andalucia Icman | Dorafshan S.,Isfahan University of Technology | Heyrati F.P.,Isfahan University of Technology | And 7 more authors.
Chemosphere | Year: 2016

Aquatic environments are the ultimate sink for most of anthropogenic pollutants. The Zayandeh Roud River is the most important river in the central Iranian Plateau, supplying water to a large population. In order to determine the potential occurrence and in vivo effects of Endocrine Disrupting Chemicals (EDCs) with estrogenic or anti-androgenic properties we analyzed the wild populations of an extensively distributed endemic fish species, Petroleuciscus esfahani. For this purpose, specimens were caught from two sites upstream and two sites downstream of the expected major anthropogenic pollution sources. P. esfahani full-length cDNAs for vitellogenin (vtg), with 4177 base pairs (bp) encoding a 1339 amino acids (aa), and for β-actin (actb), with 1776 bp encoding a 375 aa, were amplified and cloned. Hepatic vtg mRNA expression levels were measured by quantitative real-time PCR. Condition factor, gonadosomatic index and sex ratio were calculated and compared with vtg expression. Gonad histology was performed to study the possible presence of intersex condition. Detection of vtg transcripts in male individuals from the two downstream sampling sites supports the hypothesis of exposure to EDCs in these regions. Higher vtg expression in male individuals, together with reduced gonad size and condition factor, in specimens from the site located downstream of the major steel mill plant suggest a major endocrine disruption in this area. © 2015 Elsevier Ltd.


de las Heras V.,Institute Ciencias Marinas Of Andalucia Icman | de las Heras V.,Futuna Blue Espana S.L. | Martos-Sitcha J.A.,Institute Ciencias Marinas Of Andalucia Icman | Martos-Sitcha J.A.,University of Cádiz | And 3 more authors.
Aquaculture | Year: 2015

Physiological responses to different stocking densities and their subsequent effects on growth rate, energy metabolism and endocrine system were assessed in thick-lipped grey mullet (Chelon labrosus) juveniles. Three different groups of 150 fish (0.383±0.020g body mass, 101days post-hatching (dph)) were maintained in triplicate under three different stocking densities: 0.7, 2.0 and 6.7kg·m-3. All individuals were sampled at days 0, 20, 45 and 75 to obtain biometric parameters, while 25-30 specimens from each treatment were sampled for the plasma, liver and pituitary collection at the end of the experiment (75days). The lowest growth increase, both in body mass and total length, was shown in the group held at the highest stocking density (6.7kg·m-3), just by the end of the experiment (176dph). Moreover, higher plasma cortisol and glucose values were obtained in the group stocked at 0.7kg·m-3, whereas individuals maintained under the maximum density (6.7kg·m-3) had the highest hepatic glycogen and lowest glucose content. In addition, growth hormone (GH) and insulin-like growth factor (IGF-I) gene expression increased in the group maintained under the highest stocking condition. Our results indicate that C. labrosus juveniles activated both Hypothalamus-Pituitary-Interrenal (cortisol) and somatotropic (GH/IGF-I) axes to modulate metabolic and stress pathways in specimens held at different stocking densities to compensate their growth rates. © 2015 Elsevier B.V.


PubMed | Isfahan University of Technology, University of Cádiz and Institute Ciencias Marinas Of Andalucia Icman
Type: | Journal: Chemosphere | Year: 2015

Aquatic environments are the ultimate sink for most of anthropogenic pollutants. The Zayandeh Roud River is the most important river in the central Iranian Plateau, supplying water to a large population. In order to determine the potential occurrence and in vivo effects of Endocrine Disrupting Chemicals (EDCs) with estrogenic or anti-androgenic properties we analyzed the wild populations of an extensively distributed endemic fish species, Petroleuciscus esfahani. For this purpose, specimens were caught from two sites upstream and two sites downstream of the expected major anthropogenic pollution sources. P. esfahani full-length cDNAs for vitellogenin (vtg), with 4177 base pairs (bp) encoding a 1339 amino acids (aa), and for -actin (actb), with 1776 bp encoding a 375 aa, were amplified and cloned. Hepatic vtg mRNA expression levels were measured by quantitative real-time PCR. Condition factor, gonadosomatic index and sex ratio were calculated and compared with vtg expression. Gonad histology was performed to study the possible presence of intersex condition. Detection of vtg transcripts in male individuals from the two downstream sampling sites supports the hypothesis of exposure to EDCs in these regions. Higher vtg expression in male individuals, together with reduced gonad size and condition factor, in specimens from the site located downstream of the major steel mill plant suggest a major endocrine disruption in this area.


Hachero-Cruzado I.,Institute Investigacion Y Formacion Agraria Y Pesquera Of Andalucia Ifapa | Fornies A.,Institute Ciencias Marinas Of Andalucia Icman | Herrera M.,Institute Investigacion Y Formacion Agraria Y Pesquera Of Andalucia Ifapa | Mancera J.M.,University of Cádiz | Martinez-Rodriguez G.,Institute Ciencias Marinas Of Andalucia Icman
Fish Physiology and Biochemistry | Year: 2013

The aims of the present study were to characterize sperm quality and to quantify seasonal changes in sexual hormone (testosterone [T], 11-ketotestosterone [11-KT] and 17,20β-dihydroxypregn-4-en-3-one [17,20β-P]) levels in male brill (Scophthalmus rhombus) plasma, as well as to test a more intensive sampling strategy to establish relationships between sex steroid levels and sperm production parameters. Sperm concentration ranged from 0. 5 to 3. 1 × 109 spermatozoa mL-1, and changes in sperm quality parameters depending on sampling date were observed. Plasma sexual steroid levels remained high and changed in parallel during the spawning season and afterwards decreased to very low levels in summer. The analysis of annual changes of 11-KT and T ratios suggests that 11-KT can be the main circulating androgen for stimulating spermatogenesis in S. rhombus and that T could be involved in the beginning of spermatogenesis through the positive feedback on brain-pituitary-gonad axis. Finally, daily 11-KT and T levels showed similar patterns of variation in males sampled, whereas 17,20β-P amounts showed somewhat opposite trends. These differences could be related with the different role of androgens and progestin during the spermatogenesis. © 2012 Springer Science+Business Media B.V.


Riba I.,Institute Ciencias Marinas Of Andalucia Icman | Riba I.,University of Cádiz | Garcia-Luque E.,University of Cádiz | Maz-Courrau A.,University of Cádiz | And 2 more authors.
Water, Air, and Soil Pollution | Year: 2010

This study assesses the effect of salinity in bioavailability and toxicity of Zn by means of laboratory bioassays by observing contamination in both sediment and water, accumulation of Zn in biological tissues, and histopathological damage in the gills and guts tissues of Ruditapes philippinarum clams, which were exposed to different types of sediments from the Gulf of Cádiz (SW Spain) as well as two dilutions of toxic mud coming from an accidental mining spill. With this objective, the coefficients of distribution (K D) for Zn between overlying water and sediments were calculated, the histopathological frequencies in the tissues of the gills and guts of clams were determined, and the biota-sediment bioaccumulation factors as well as the bioaccumulation factors were quantified in the different stations. Results showed that the greatest histopathological damages appeared when the salinity values decreased. Statistical results showed that salinity was inversely correlated with histopathological damage (p<0.01) for the lesion index for gills. The most outstanding results were observed in the two dilutions of toxic mud (0.3% and 7.9%) at a salinity value of 10. Salinity was inversely correlated with the concentration of Zn in biological tissues (p<0.05) and inversely correlated with the concentration of Zn in water and sediment. Zn mobilization to the overlying water is produced when salinity values decrease. © 2010 Springer Science+Business Media B.V.


Marco-Herrero E.,Institute Ciencias Marinas Of Andalucia Icman | Guerao G.,IRTA - Institute of Agricultural-Alimentary Research and Technology | Cuesta J.A.,Institute Ciencias Marinas Of Andalucia Icman
Scientia Marina | Year: 2013

In this study the complete larval development (including four zoeal stages and a megalopa stage) of Dyspanopeus sayi is described and illustrated in detail based on larvae reared in the laboratory. Ovigerous females were collected in Alfacs Bay, Ebro Delta (NW Mediterranean) where the species was recently introduced. These larval stages were compared with others collected in the plankton from the same area, the larval stages described previously for this species and the larval stages of the two other Panopeidae that inhabit the Iberian Peninsula: Panopeus africanus and Rhithropanopeus harrisii. Differences were found in both zoeal and megalopal larval characters between D. sayi and the other two species, which could raise doubt about their placement in the same family.


Martos-Sitcha J.A.,University of Cádiz | Martos-Sitcha J.A.,Institute Ciencias Marinas Of Andalucia Icman | Wunderink Y.S.,University of Cádiz | Gozdowska M.,Polish Academy of Sciences | And 3 more authors.
Comparative Biochemistry and Physiology - A Molecular and Integrative Physiology | Year: 2013

To investigate the physiological roles of arginine vasotocin (AVT) and isotocin (IT) in osmoregulatory process in gilthead sea bream (Sparus aurata), a time course study (0, 12. h, and 1, 3, 7 and 14. days) has been performed in specimens submitted to hypoosmotic (from 40‰ salinity to 5‰ salinity) or hyperosmotic (from 40‰ salinity to 55‰ salinity) challenges. Plasma and liver osmoregulatory and metabolic parameters, as well as AVT and IT pituitary contents were determined concomitantly with hypothalamic pro-vasotocin (pro-VT) and pro-isotocin (pro-IT) mRNA expression levels. Previously, sequences coding for pro-VT and pro-IT cDNAs were cloned. Two osmoregulatory periods related to plasma osmolality and metabolic parameter variations could be distinguished: i) an adaptative period, from 12. h to 3. days after transfer, and ii) a chronic regulatory period, starting at day 3 after transfer. Higher values in hypothalamic pro-VT and pro-IT mRNA expression as well as in pituitary AVT and IT storage levels in both hypo- and/or hyper-osmotic transfers have been distinguished. These increase correlated with changes in plasma cortisol levels, suggesting an interaction between this hormone and pro-VT expression. Furthermore, pro-IT expression enhancement also suggests a role of the isotocinergic system as a modulator in the acute stress response induced by hyper-osmotic challenge in S. aurata. © 2013 Elsevier Inc.


PubMed | Institute Ciencias Marinas Of Andalucia Icman
Type: Journal Article | Journal: Journal of fish diseases | Year: 2013

The transmission of lymphocystis disease virus (LCDV) to gilthead seabream, Sparus aurata L., larvae was investigated using fertilized eggs from a farm with previous reports of lymphocystis disease. LCDV genome was detected by PCR-hybridization in blood samples from 17.5% of the asymptomatic gilthead seabream broodstock analysed. Using the same methodology, eggs spawned from these animals were LCDV positive, as well as larvae hatched from them. The presence of infective viral particles was confirmed by cytopathic effects development on SAF-1 cells. Whole-mount in situ hybridization (ISH) and immunohistochemistry (IHC) showed the presence of LCDV in the epidermis of larvae hatched from LCDV-positive eggs. When fertilized eggs were disinfected with iodine, no viral DNA was detected either in eggs (analysed by PCR-hybridization) or in larvae (PCR-hybridization and ISH). These results suggest the vertical transmission of LCDV, the virus being transmitted on the egg surface. Larvae hatched from disinfected eggs remain LCDV negative during the endotrophic phase, as showed by PCR-hybridization, ISH and IHC. After feeding on LCDV-positive rotifers, viral antigens were observed in the digestive tract, which suggests that viral entry could be achieved via the alimentary canal, and that rotifers can act as a vector in LCDV transmission to gilthead seabream larvae.

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