Institute Ciencias Do Mar

Fortaleza, Brazil

Institute Ciencias Do Mar

Fortaleza, Brazil

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Barreto R.V.G.,Federal University of Ceará | Barreto R.V.G.,Institute Ciencias do Mar | Hissa D.C.,Federal University of Ceará | Paes F.A.,Federal University of Ceará | And 7 more authors.
Bioresource Technology | Year: 2010

Spores of Bacillus subtilis LAMI008 were entrapped in 3-mm chitosan beads and cross-linked with 0.3% glutaraldehyde for n-hexadecane biodegradation and biosurfactant recovery. When exposed to nutrients, the spores generated vegetative cells without morphological alterations as revealed by atomic force microscopy. The entrapped cells degraded almost 100% of 1% of n-hexadecane in medium supplemented with 1% glucose and produce biosurfactant within 48 h, as well as free cells. The number of viable cells inside the beads was maintained throughout the n-hexadecane degradation process and the released biosurfactant was not used as a carbon source. Entrapment of bacterial spores in chitosan beads overcomes problems with stability, storage, and long term cell viability encountered with vegetative cells. This approach can potentially be utilized for biodegradation of complex compounds by entrapping spores of different species of bacteria. © 2009 Elsevier Ltd. All rights reserved.


de Melo L.M.R.,Potiguar University | Almeida D.,Federal University of Rio Grande do Norte | Hofer E.,Instituto Oswaldo Cruz | dos Reis C.M.F.,Instituto Oswaldo Cruz | And 3 more authors.
Brazilian Journal of Microbiology | Year: 2011

Ten out of fifty fresh and refrigerated samples of shrimp (Litopenaeus vannamei) collected from retailers in Natal (Rio Grande do Norte, Northeastern Brazil) tested positive for Vibrio parahaemolyticus. The Kanagawa test and multiplex PCR assays were used to detect TDH and TRH hemolysins and the tdh, trh and tlh genes, respectively. All strains were Kanagawa-negative and tlh-positive. Antibiotic susceptibility testing was done for seven antibiotics by the agar diffusion technique. Five strains (50%) presented multiple antibiotic resistance to ampicillin (90%) and amikacin (60%), while two strains (20%) displayed intermediate-level resistance to amikacin. All strains were sensitive to chloramphenicol. Intermediate-level susceptibility and/or resistance to other antibiotics ranged from 10 to 90%, with emphasis on the observed growing intermediate-level resistance to ciprofloxacin. Half our isolates yielded a multiple antibiotic resistance index above 0.2 (range: 0.14-0.29), indicating a considerable risk of propagation of antibiotic resistance throughout the food chain.


Tavares T.C.L.,Federal University of Ceará | Tavares T.C.L.,Institute Ciencias do Mar | Nogueira V.L.R.,Federal University of Ceará | Nogueira V.L.R.,Institute Ciencias do Mar | And 6 more authors.
Journal of Experimental Marine Biology and Ecology | Year: 2011

Sea hares are well known, nearly shell-less, marine opisthobranchs that use a complex repertoire of chemicals for defense and communication instead of a conventional gastropod shell. The most conspicuous characteristic of these invertebrates is the secretion of ink, which is rich in bioactive proteins. Many of these proteins belong to a family of L-amino acid oxidases (L-AAOs). In the current study, we aimed to determine whether dactylomelin-P, an antibacterial protein isolated from the ink of Aplysia dactylomela, could act as an L-AAO. We also investigated its biochemical properties and antibacterial mechanism of action. We found that dactylomelin-P is an acidic protein (pI=5.0), rich in glutamic acid/glutamine, aspartic acid/asparagine, tyrosine, serine, and proline. It was stable under a broad pH range (3.0-12.0), after heating to 55°C for 30min, and after treating with protease. Its N-terminal amino acid sequence was DGVCSNRRQCNKEVCGSSYDVAIVGA and showed high similarity to other sea hare proteins previously identified as L-AAOs. The L-AAO activity was confirmed in an enzymatic assay, which showed that dactylomelin-P could oxidize L-lysine and L-arginine. We also demonstrated that the bacteriostatic activity of dactylomelin-P was mediated by hydrogen peroxide generated in the enzymatic reaction, but it acted as a bactericide in the presence of L-lysine and L-arginine. Transmission electron microscopy analyses showed that dactylomelin-P bound to growth-phase bacteria without causing morphological alterations to the cells. The bactericidal effect seems to involve H2O2 and other reactive components since it was not counteracted by H2O2 scavengers. Our findings showed biochemical, functional, and phylogenetic similarities among L-AAOs isolated from sea hares; this offers new insight into the evolution of these proteins and their roles in chemical defense. © 2011 Elsevier B.V.


Lacerda L.D.,Institute Ciencias Do Mar | Costa B.G.B.C.,Institute Ciencias Do Mar | Lopes D.N.,Institute Ciencias Do Mar | Oliveira K.,Institute Ciencias Do Mar | And 2 more authors.
Bulletin of Environmental Contamination and Toxicology | Year: 2014

Indigenous, introduced and farmed fish species were collected from the Jaguaribe Basin, NE Brazil to assess total and methyl mercury concentrations in muscle tissue and to determine its relationship with fish origin. The results obtained from introduced species were compared to the Hg content of their original area of occurrence, which is located in the Amazon region. Farmed and wild individuals of Oreochromis niloticus (tilapia) were also compared. Introduced species presented lower Hg contents compared to their Amazonian counterparts. Wild individuals of O. niloticus also presented higher Hg contents than farmed individuals with similar sizes. However, methyl Hg concentrations were not significantly different between the two groups. Total Hg and methyl Hg concentrations were higher in carnivorous species, especially those from the Amazon region, and surpassed the Brazilian guidelines for fish consumption. Based on their Hg content, Jaguaribe basin fish do not pose a significant threat to human consumption. © 2014 Springer Science+Business Media New York.


Cruz R.,Institute Ciencias Do Mar | Silva K.C.A.,University of Amazon | Santana J.V.M.,Instituto Federal Of Educacao | Gaeta J.C.,Institute Ciencias Do Mar | Cintra I.H.A.,University of Amazon
Crustaceana | Year: 2014

The spiny lobster Panulirus argus (Latreille, 1804) is the most important commercial fishing resource in the western central Atlantic and Brazil. Field studies covering the waters off southwestern Cuba and northern Brazil have improved our understanding of the variations in the reproductive potential (number of eggs), stock recruitment and reproductive efficiency of spiny lobsters according to location, depth and size class. Using the spawner-recruitment model, the reproductive potential index was correlated with the index of subsequent recruitment based on field sampling. Spiny lobster habitats in deeper waters need special attention in order to protect the species from overfishing of the recruitment. Considering the longevity and absence of reproductive senility in spiny lobsters, management strategies should ideally include the creation of spawner sanctuaries (marine protected areas) capable of restoring and maintaining the biomass of the spawning stock and the establishment of a maximum catch size of 135 mm (CL) for both sexes along the entire Brazilian coast. Based on our findings, we propose to establish spiny lobster sanctuaries (50-100 m) on the continental shelf off northern Brazil, from Amapá (5°25′N 51°0′W) to the western reaches of the coast of Pará (1°11′N 46°27′W, 0°42′N 46°45′W), covering a total surface area of 64 230 km2. © 2014 Koninklijke Brill NV, Leiden.


Bezerra M.G.V.,Secretaria Municipal de Saude de Eusebio | Rigotto R.M.,Federal University of Ceará | Pessoa V.M.,Fundacao Oswaldo Cruz | da Silva F.V.E.,Institute Ciencias do Mar
Ciencia e Saude Coletiva | Year: 2014

This paper discusses aspects of economic development and the implications on work, the environment and health in the surrounding communities of the Industrial and Harbor Complex in Pecém in the State of Ceará. Qualitative research was adopted as the methodological strategy, by conducting participatory research with document analysis and a focus group. The reports of the subjects involved in the fieldwork were analyzed as being representative of their perceptions regarding the changes occurring in the territory and the impacts on health. Results observed in the use and appropriation of land by entrepreneurs are based on a belief in progress and development, contradicting the way of living, producing and interacting with nature submitted by the community that seeks to resist this intervention supported by social movements. These changes are out of step with the development of other public policies to mitigate the impacts with regard to environmental protection of the territory and the promotion of the health of this population. © 2014, Associacao Brasileira de Pos - Graduacao em Saude Coletiva. All rights Reserved.


Sousa O.V.,Institute Ciencias do Mar | Evangelista-Barreto N.S.,Federal University of Recôncavo da Bahia | Catter K.M.,State University of Ceará | Fonteles-Filho A.A.,Institute Ciencias do Mar | And 2 more authors.
Journal of Water and Health | Year: 2010

Defined substrate (DS) is an alternative technique to monitoring the water quality based on species-specific enzyme activity. Although more sensitive and more specific than traditional media, there is some controversy over use in the warmer waters of tropical and subtropical environments, rich in organic matter and microorganism groups capable of interfering with results. The aim of this study was to test the specificity of DS method (Colilert, IDEXX) for detection of coliforms and Escherichia coli in stormwater seawater samples from a coastal city (Fortaleza, Brazil) compared to findings obtained with the multiple tube fermentation (MTF) method. The samples were collected from stormsewers and adjacent seashore locations. The most probable number (MPN) of total coliforms (TC), thermotolerant coliforms (TtC) and E. coli was determined and the selectivity of the enzymatic substrate medium in the seawater samples was tested. The MTF method showed samples from sampling points 1, 2 and 3 to be 13.3, 13.3 and 46.7%, respectively, above the legal cut-off value for coastal balneability. With the DS method, the corresponding figures were 60, 53.3 and 80% for E. coli. Overall, coliform levels were higher with the DS medium. Vibrios and aeromonads were isolated from E. coli-positive DS tubes. © IWA Publishing 2010.


Tavares T.C.L.,Institute Ciencias do Mar | Tavares T.C.L.,Federal University of Ceará | Normando L.R.O.,Federal University of Ceará | de Vasconcelos A.T.R.,Laboratorio Of Bioinformatica | And 4 more authors.
Science of the Total Environment | Year: 2016

Maritime ports are anthropogenic interventions capable of causing serious alterations in coastal ecosystems. In this study, we examined the benthic microbial diversity and community structure under the influence of two maritime ports, Mucuripe (MUC) and Pecém (PEC), at Equatorial Atlantic Ocean in Northeast Brazil. Those seaports differ in architecture, time of functioning, cargo handling and contamination. The microbiomes from MUC and PEC were also compared in silico to 11 other globally distributed marine microbiomes. The comparative analysis of operational taxonomic units (OTUs) retrieved by PCR-DGGE showed that MUC presents greater richness and β diversity of Bacteria and Archaea than PEC. In line with these results, metagenomic analysis showed that MUC and PEC benthic microbial communities share the main common bacterial phyla found in coastal environments, although can be distinguish by greater abundance of Cyanobacteria in MUC and Deltaproteobacteria in PEC. Both ports differed in Archaea composition, being PEC port sediments dominated by Thaumarchaeota. The microbiomes showed little divergence in their potential metabolic pathways, although shifts on the microbial taxonomic signatures involved in nitrogen and sulphur metabolic pathways were observed. The comparative analysis of different benthic marine metagenomes from Brazil, Australia and Mexico grouped them by the geographic location rather than by the type of ecosystem, although at phylum level seaport sediments share a core microbiome constituted by Proteobacteria, Cyanobacteria, Actinobacteria, Tenericuteres, Firmicutes, Bacteriodetes and Euryarchaeota. Our results suggest that multiple physical and chemical factors acting on sediments as a result of at least 60 years of port operation play a role in shaping the benthic microbial communities at taxonomic level, but not at functional level. © 2016 Elsevier B.V.


Lefevre N.,University Pierre and Marie Curie | Lefevre N.,Institute Ciencias Do Mar | Urbano D.F.,National Institute for Space Research | Gallois F.,CIRAD - Agricultural Research for Development | Diverres D.,Center Ird Of Bretagne
Journal of Geophysical Research: Oceans | Year: 2014

The fugacity of CO2 (fCO2) has been measured underway during three quasi-synoptic cruises in the western tropical Atlantic in March/April 2009 and July/August 2010 in the region 6°S-15°N, 52°W-24°W. The distribution of fCO2 is related to the main features of the ocean circulation. Temperature exerts a dominant control on the distribution of fCO2 in March/April whereas salinity plays an important role in July/August due to the more developed North Equatorial Countercurrent (NECC) carrying Amazon water and to the high precipitation associated with the presence of the Intertropical Convergence Zone (ITCZ). The main surface currents are characterized by different fCO2. Overall, the NECC carries less saline waters with lower fCO2 compared to the South Equatorial Current (SEC). The North Equatorial Current (NEC) is usually characterized by CO2 undersaturation in winter and supersaturation in summer. Using empirical fCO2-SST-SSS relationships, two seasonal maps of fCO2 are constructed for March 2009 and July 2010. The region is a sink of CO2 of 0.40 mmol m-2d-1 in March, explained by the winter cooling in the northern hemisphere, whereas it is a source of CO2 of 1.32 mmol m-2d-1 in July. The equatorial region is a source of CO2 throughout the year due to the upwelling supplying CO2-rich waters to the surface. However, the evolution of fCO2 over time, determined from all the available cruises in a small area, 1°S-1°N, 32°W-28°W, suggests that the source of CO2 has decreased in February-March from 1983 to 2011 or has remained constant in October-November from 1991 to 2010. Key Points Seasonal CO2 maps from synoptic cruises are produced Surface fCO2 is mainly driven by temperature and salinity The equatorial source of CO 2 is decreasing or constant over time ©2014. American Geophysical Union. All Rights Reserved.


PubMed | Institute Ciencias do Mar
Type: Journal Article | Journal: Journal of water and health | Year: 2010

Defined substrate (DS) is an alternative technique to monitoring the water quality based on species-specific enzyme activity. Although more sensitive and more specific than traditional media, there is some controversy over use in the warmer waters of tropical and subtropical environments, rich in organic matter and microorganism groups capable of interfering with results. The aim of this study was to test the specificity of DS method (Colilert, IDEXX) for detection of coliforms and Escherichia coli in stormwater seawater samples from a coastal city (Fortaleza, Brazil) compared to findings obtained with the multiple tube fermentation (MTF) method. The samples were collected from stormsewers and adjacent seashore locations. The most probable number (MPN) of total coliforms (TC), thermotolerant coliforms (TtC) and E. coli was determined and the selectivity of the enzymatic substrate medium in the seawater samples was tested. The MTF method showed samples from sampling points 1, 2 and 3 to be 13.3, 13.3 and 46.7%, respectively, above the legal cut-off value for coastal balneability. With the DS method, the corresponding figures were 60, 53.3 and 80% for E. coli. Overall, coliform levels were higher with the DS medium. Vibrios and aeromonads were isolated from E. coli-positive DS tubes.

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