Functional studies of TcRjl, a novel GTPase of Trypanosoma cruzi, reveals phenotypes related with MAPK activation during parasite differentiation and after heterologous expression in Drosophila model system
Dos-Santos G.R.R.M.,Federal University of Rio de Janeiro |
Fontenele M.R.,Desenvolvimento Institute Ciencias Biomedicas |
Dias F.D.A.,Institute Bioquimica Medica |
Oliveira P.L.D.,Institute Bioquimica Medica |
And 7 more authors.
Biochemical and Biophysical Research Communications | Year: 2015
The life cycle of the protozoan parasite Trypanosoma cruzi comprises rounds of proliferative cycles and differentiation in distinct host environments. Ras GTPases are molecular switches that play pivotal regulatory functions in cell fate. Rjl is a novel GTPase with unknown function. Herein we show that TcRjl blocks in vivo cell differentiation. The forced expression of TcRjl leads to changes in the overall tyrosine protein phosphorylation profile of parasites. TcRjl expressing parasites sustained DNA synthesis regardless the external stimuli for differentiation. Heterologous expression in the Drosophila melanogaster genetic system strongly suggests a role from TcRjl protein in RTK-dependent pathways and MAPK activation. © 2015 Elsevier Inc. Source