Da Silva J.S.,Institute Ciencias Ambientais |
Junqueira H.C.,University of Sao Paulo |
Ferreira T.L.,Institute Ciencias Ambientais
Electrochimica Acta | Year: 2014
Microelectrode voltammetry was employed to gain information on the n-octanol/water partition ratio of phenothiazine dyes. The data were obtained as a function of pH and dye concentration in aqueous solutions. All the dyes present hydrophobic behavior at pH above 7.0. The analysis of the results shows not only the formation of neutral species in alkaline solution but the association of cationic form of these dyes with OH-play important roles in the partitioning of molecules from aqueous solution to n-octanol. © 2014 Elsevier Ltd. All rights reserved.
Costa L.T.,Federal University of Alfenas |
Siqueira L.J.A.,Institute Ciencias Ambientais |
Nicolau B.G.,University of Sao Paulo |
Ribeiro M.C.C.,University of Sao Paulo
Vibrational Spectroscopy | Year: 2010
Raman spectra of polymer electrolytes based on poly(ethylene glycol) dimethyl ether (PEGdME) with LiClO 4, PEGdME/LiClO 4, and the ionic liquid 1-butyl-3-methylimidazolium hexafluorophosphate, PEGdME/[bmim]PF 6, are compared. Raman spectroscopy suggests stronger interactions in PEGdME/LiClO 4 than PEGdME/[bmim]PF 6, thus corroborating previous results obtained by molecular dynamics simulations. Quantum Chemistry methods have been used to calculate vibrational frequencies and the equilibrium structure of segments of the polymer chain around the cation. A consistent picture has been obtained from Raman spectroscopy, density functional theory (DFT) calculations, and molecular dynamics simulations for these polymer electrolytes. © 2010 Elsevier B.V. All rights reserved.
Lima A.S.,University of Sao Paulo |
Salles M.O.,University of Sao Paulo |
Ferreira T.L.,Institute Ciencias Ambientais |
Paixao T.R.L.C.,University of Sao Paulo |
Bertotti M.,University of Sao Paulo
Electrochimica Acta | Year: 2012
The cathodic reduction of nitrate at copper electrodes was investigated by using scanning electrochemical microscopy (SECM) in the stripping-mode SECM (SM-SECM). This electrochemical process is facilitated at surfaces activated by a potential protocol involving anodic dissolution and subsequent reduction of copper cations, which causes an increase in the surface area. In the presence of chloride, a much more significant current enhancement was noticed, and a mechanism based on the formation of CuCl was proposed to explain the increased rate of nitrate reduction at this experimental condition. © 2012 Elsevier Ltd.
Giavarotti L.,University of Sao Paulo |
Simon K.A.,Institute Ciencias Ambientais |
Azzalis L.A.,Institute Ciencias Ambientais |
Fonseca F.L.A.,Institute Ciencias Ambientais |
And 8 more authors.
Oxidative Medicine and Cellular Longevity | Year: 2013
Alzheimer's disease (AD) is a late-onset, progressive degenerative disorder that affects mainly the judgment, emotional stability, and memory domains. AD is the outcome of a complex interaction among several factors which are not fully understood yet; nevertheless, it is clear that oxidative stress and inflammatory pathways are among these factors. 65 elderly subjects (42 cognitively intact and 23 with probable Alzheimer's disease) were selected for this study. We evaluated erythrocyte activities of superoxide dismutase, catalase, and glutathione peroxidase as well as plasma levels of total glutathione, α-tocopherol, β-carotene, lycopene, and coenzyme Q10. These antioxidant parameters were confronted with plasmatic levels of protein and lipid oxidation products. Additionally, we measured basal expression of monocyte HLA-DR and CD-11b, as well as monocyte production of cytokines IL1-α, IL-6, and TNF-α. AD patients presented lower plasmatic levels of α-tocopherol when compared to control ones and also higher basal monocyte HLA-DR expression associated with higher IL-1α production when stimulated by LPS. These findings support the inflammatory theory of AD and point out that this disease is associated with a higher basal activation of circulating monocytes that may be a result of α-tocopherol stock depletion. © 2013 Leandro Giavarotti et al.
Santos R.T.D.,University of Sao Paulo |
Longo L.S.,Institute Ciencias Ambientais |
Alves S.,Institute Ciencias Ambientais
Molecular Crystals and Liquid Crystals | Year: 2014
In this work, we described the nonlinear optical properties from thermal origin of lamellar liquid crystals by Z-scan technique in different regions of the phase diagram. The results showed that nonlinear refractive index of LLC depends on the solvent layer space. We also studied the influence of the addition of magnetic nanoparticles in nonlinear optical properties of the system. © 2014 Copyright © Taylor & Francis Group, LLC.
PubMed | Laboratorio Of Analises Clinicas, Institute Ciencias Ambientais and Disciplina de Dermatologia
Type: Journal Article | Journal: Journal of cosmetic dermatology | Year: 2016
Acne in adult women is a frequent hard-to-manage disease with many relapse cases. It mostly interferes with the quality of life of patients, bringing them major metabolic and social losses. As androgenic hormones play a very important role in the acne pathogenesis, the early diagnosis of hyperandrogenic states is very useful for the proper evaluation of each patient and for a better choice of therapeutic management. Defining a pattern for laboratory profile analysis is important for the control of relapses of acne breakouts in adult women, which lately has been the aim of many published studies.To establish the relation between 3 alpha-diol G levels and acne in female patients with normal androgenic status without menstrual dysfunctions.The evaluation of serum 3 alpha-androstanediol glucuronide levels through an enzymatic immunoassay method (Androstanediol Glucuronide ELISA Kit) for a direct quantitative measurement in 26 patients with grade II and III acne, ages ranging from 13 to 50.Among the analyzed patients, 83% had grade II acne, and among this total, 60% were aged 14 or over. According to age, 12 studied patients showed serum 3 alpha-diol G levels within normal range and 11 patients had increased levels.A total of 60% of adult women with acne present increased levels of androgens and among those with normal levels and without menstrual dysfunctions, 50% show an increase in 3 alpha-diol G. Therefore, a pharmacological approach with anti-androgenic drugs for acne therapy in most of these patients is advisable.
Perez C.,Institute Ciencias Ambientais |
Simoes F.R.,Unifesp Campus Baixada Santista |
Codognoto L.,Institute Ciencias Ambientais
Journal of Solid State Electrochemistry | Year: 2015
In this work, a simple method for electroanalytical determination of 17α-ethinylestradiol (EE2) hormone in natural waters was developed using a boron-doped diamond electrode (BDD). The analyses were performed using square wave voltammetry and the parameters were optimized. The results showed a well-defined irreversible oxidation peak (BR buffer 0.1 mol L−1, pH 8.0) at +0.65 V (vs. Ag/AgCl). The voltammetric results showed also that the oxidation process is controlled by adsorption of species and indicated that there are two electrons involved. The obtained analytical curves for 17α-ethinylestradiol presented good linearity in the concentration range 9.9 × 10−7 to 5.2 × 10−6 mol L−1 in utlrapure water and 7.9 × 10−7 to 5.2 × 10−6 mol L−1 in natural water samples (supply dam). Detection limits (DL) obtained were between 2.4 × 10−7 and 7.5 × 10−7 mol L−1 and quantification limits (QL) between 7.9 × 10−7 and 2.5 × 10−6 mol L−1. The recovery experiments showed values between 86 and 114 % for spiked samples thus indicating the applicability of the electroanalytical methodology to quantify 17α-ethinylestradiol directly in natural water of supply Dam (Billings Dam in Diadema-SP. Brazil), without any preconcentration or derivatization. © 2015 Springer-Verlag Berlin Heidelberg
Correa M.F.,Institute Ciencias Ambientais |
Fernandes J.P.D.S.,Institute Ciencias Ambientais
Chemical Biology and Drug Design | Year: 2015
Histamine is a chemical transmitter found practically in whole organism and exerts its effects through the interaction with H1 to H4 histaminergic receptors. Specifically, H4 receptors are found mainly in immune cells and blood-forming tissues, thus are involved in inflammatory and immune processes, as well as some actions in central nervous system. Therefore, H4 receptor ligands can have applications in the treatment of chronic inflammatory and immune diseases and may be novel therapeutic option in these conditions. Several H4 receptor ligands have been described from early 2000's until nowadays, being imidazole, indolecarboxamide, 2-aminopyrimidine, quinazoline, and quinoxaline scaffolds the most explored and discussed in this review. Moreover, several studies of molecular modeling using homology models of H4 receptor and QSAR data of the ligands are summarized. The increasing and promising therapeutic applications are leading these compounds to clinical trials, which probably will be part of the next generation of blockbuster drugs. © 2014 John Wiley & Sons A/S.
De Abreu C.C.,Federal University of ABC |
Pires Rosa P.C.,University of Campinas |
Da Costa Aguiar Alves B.,Federal University of ABC |
Ajaime Azzalis L.,Institute Ciencias Ambientais |
And 6 more authors.
European Review for Medical and Pharmacological Sciences | Year: 2015
OBJECTIVE: The acute lymphocytic leukemia is a hematopoietic cancer that occurs predominantly in children. Methotrexate is one of the most useful drugs in cancer chemotherapy. The aim of the study was to develop and validate the methodology of high performance liquid chromatography (HPLC) with ultraviolet detection for methotrexate dosage and to determine its concentration in plasma samples from children with leukemia. PATIENTS AND METHODS: The study included patients from the outpatient care of pediatric oncology at the Faculty of Medicine of ABC carriers in treatment of leukemia. The study was conducted in chromatographic model Agilent 1100 with UV detector at 302 nm and by the method of ELISA microplate reader capable of reading absorbance at 450 nm. RESULTS: We obtained satisfactory results of selectivity, accuracy, linearity, limit of quantification (LOQ), limit of detection (LOD), precision and robustness and apply the basic criteria for validation as RE No. 899, of May 29, 2003 Guide validation of analytical and bioanalytical National Agency Health Surveillance (ANVISA). CONCLUSIONS: We conclude that results for linearity/concentration range, precision, robustness, limit of quantification and detection limits are within the acceptance criteria defined by ANVISA and that the developed analytical method is valid and feasible to be used as a tool in monitoring therapy of methotrexate.