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Correa-Calderon A.,Institute Ciencias Agricolas | Perez-Velazquez R.,Institute Ciencias Agricolas | Avendano-Reyes L.,Institute Ciencias Agricolas | Macias-Cruz U.,Institute Ciencias Agricolas | And 2 more authors.
Animal science journal = Nihon chikusan Gakkaiho | Year: 2016

To investigate the effects of progesterone supplementation at two different times on serum progesterone (P4 ) concentration, conception rate and resynchronization of cooled Holstein heifers in summer, 90 heifers were randomly assigned to two groups: (i) heifers subjected to TAI (timed artificial insemination) and progesterone supplementation from days 4 to 14 after TAI (S1; n = 45); and (ii) heifers under the same TAI protocol as S1 and progesterone supplementation from days 17 to 22 after TAI (S2 ; n = 45). The groups S1 and S2 were cooled 10 days before and 21 days after TAI. Respiratory rate, body surface temperature, vaginal temperature and rectal temperature recorded during the experiment were not different (P > 0.05) between S1 and S2 groups. Progesterone concentration was not different (P > 0.05) in S1 compared to S2 . The conception rates on days 30 and 55 were similar between groups (P > 0.05). Progesterone supplementation did not increase either conception rate or concentrations of P4 in heifers during the summer. Heifers not pregnant to first service in the group S2 were resynchronized (77.7%) for a second breeding. © 2015 Japanese Society of Animal Science.


Morales A.,Institute Ciencias Agricolas | Araiza A.B.,Institute Ciencias Agricolas | Zijlstra R.T.,University of Alberta | Bernal H.,Autonomous University of Nuevo León | Cervantes M.,Institute Ciencias Agricolas
Journal of Animal Physiology and Animal Nutrition | Year: 2013

Summary: An experiment was conducted to evaluate the effect of excess levels of Leu and Lys on the expression of b0,+ and CAT-1 mRNA in jejunum, liver and the muscles Longissimus dorsi (LDM) and Semitendinosus (STM). Twenty pigs with an average initial BW of 16.4±1.7kg were used in a Randomized Complete Block. Dietary treatments (T) were as follows: T1, basal diet; T2, basal plus 3.5g l-Lys/kg diet; T3, basal plus 1.5g l-Leu/kg diet; T4, basal plus 3.5g l-Lys plus 1.5g l-Leu/kg diet. Diets in T1 and T3 met 100% the requirement of Lys for pigs within the 10 to 20kg body weight range; diets in T2 and T4 contained 35% excess of Lys. Also, diets in T1 and T2 supplied 104%, whereas diets in T3 and T4 supplied 116% the requirement of Leu. The expression of b0,+ in jejunum was reduced (p=0.002) because of the supplementation of l-Leu, but l-Lys supplementation had no effect (p=0.738). In contrast, the expression of b0,+ in STM (p=0.012) and liver (p=0.095) was reduced by the high level of Lys, but Leu had no effect (p>0.100). CAT-1 expression in STM increased by high Lys (p=0.023) and Leu (p=0.007) levels. In liver, the expression of CAT-1 substantially increased (p=0.001) because of Lys. In conclusion, excess levels of dietary Lys and Leu affect the expression of cationic amino acid transporters, and this effect varies depending on the studied tissue. © 2011 Blackwell Verlag GmbH.


Bernal-Alzate J.,Institute Ciencias Agricolas | Grimaldo-Juarez O.,Institute Ciencias Agricolas | Gonzalez-Mendoza D.,Institute Ciencias Agricolas | Cervantes-Diaz L.,Institute Ciencias Agricolas | And 2 more authors.
Idesia | Year: 2016

The green beans are part of the basic diet in Latin America; however, his yield is slowed down by several factors and the growers demands new alternatives for diseases management and increase yields of varieties. The aim of this work was to evaluate the yield of two green beans cultivars grafted onto two varieties of runner bean (Phaseolus coccineous) rootstocks. The grafting was spliced side type and the success of the procedure was observed at 20th day. At 7, 21 and 56 days after transplantation plant height was measured. At harvest, pod number, pod length and yield per plant was measured. The percentage of surviving results showed an early compatibility in most treatments except treatment FXO, provided that no response to the graft. Grafted treatments increased 67.9% in length of the plants compared with non-grafted plants, fruit size and yield also increased by 50% and 100% respectively. The green bean grafted on to runner bean represents an alternative to improve the yield and quality of fruit. © 2016, Universidad de Tarapaca. All rights reserved.


Ponce J.L.,Institute Ciencias Agricolas | Macias U.,Institute Ciencias Agricolas | Alvarez F.D.,Institute Ciencias Agricolas | Avendano L.,Institute Ciencias Agricolas | Ly J.,Institute Investigaciones Porcinas
Cuban Journal of Agricultural Science | Year: 2013

A study was developed including low doses of gonadotropin chorionic equine (GCe), the ewes' genotype and the production value of the lambs at weaning which were the offspring of hair sheep ewes. Thirty nine multipara ewes of the genotype Pelibuey purebred and the Pelibuey x Romanov cross, were synchronized using intravaginal sponges impregnated with flurogestone acetate (FGA) for 12 d. At the eleventh day of treatment, ewes of each genotype were divided in two groups and injected with 140 or 280 immunizer units (IU) of GCe. A total of two matings corresponded to each female, with 12 h interval between each one. A completely randomized design with a 22 factorial arrangement was applied to analyze the information. None of the variables was affected (P > 0.05) by the interaction dose x genotype or by the main effect of GCe dose. The production value was 25% higher in Pelibuey ewes than in Pelibuey x Romanov. Likewise it was in 32% of ewes treated with 280 IU regarding those receiving 140 IU of GCe. It is concluded that production value of the lamb at weaning is the same in Pelibuey and Pelibuey x Romanov ewes, treated with doses of 140 or 280 IU of GCe.


Garcia-Villalobos H.,Institute Ciencias Agricolas | Morales-Trejo A.,Institute Ciencias Agricolas | Araiza-Pina B.A.,Institute Ciencias Agricolas | Htoo J.K.,Evonik Industries | Cervantes-Ramirez M.,Institute Ciencias Agricolas
Archives of Animal Nutrition | Year: 2012

The absorption of lysine is facilitated by leucine, but there is no information regarding the effect of crude protein, lysine and leucine levels on the expression of cationic amino acid transporters in pigs. Therefore, an experiment was conducted with 20 pigs (14.9 ± 0.62 kg initial body weight) to evaluate the effect of two protein levels, and the content of lysine, threonine, methionine and leucine in low crude protein diets on the expression of b0,+ and CAT-1 mRNA in jejunum, Longissimus dorsi and Semitendinosus muscles and serum concentration of amino acids. Treatments were as follows: (i) wheat-soybean meal diet, 20% crude protein (Control); (ii) wheat diet deficient in lysine, threonine and methionine (Basal diet); (iii) Basal diet plus 0.70% l-lysine, 0.27% l-threonine, 0.10% dl-methionine (Diet LTM); (iv) Diet LTM plus 0.80% l-leucine (Diet LTM + Leu). Despite the Basal diet, all diets were formulated to meet the requirements of lysine, threonine and methionine; Diet LTM + Leu supplied 60% excess of leucine. The addition of lysine, threonine and methionine in Diet LTM increased the expression of b0,+ in jejunum and CAT-1 in the Semitendinosus and Longissiums muscles and decreased CAT-1 in jejunum; the serum concentration of lysine was also increased (p < 0.01). Further addition of l-leucine (Diet LTM + Leu) decreased the b0,+ expression in jejunum and CAT-1 in the Longissimus dorsi muscle (p < 0.05), increased the serum concentration of leucine and arginine and decreased the concentration of isoleucine (p < 0.05). Pigs fed the Control diet expressed less b0,+ in jejunum, and CAT-1 in the Semitendinosus and Longissiums muscles expressed more CAT-1 in jejunum (p < 0.05) and had lower serum concentration of isoleucine, leucine and valine (p < 0.05), but higher lysine concentrations (p < 0.01) than those fed Diet LTM. These results indicated that both, the level and the source of dietary amino acids, affect the expression of cationic amino acid transporters in pigs fed wheat-based diets. © 2012 Copyright Taylor and Francis Group, LLC.


Moncada-Aguilar A.M.,Institute Ciencias Agricolas | Ramirez-Hernandez J.,Institute Ingenieria | Quintero-Nunez M.,Institute Ingenieria | Avendano-Reyes L.,Institute Ciencias Agricolas
Water, Air, and Soil Pollution | Year: 2010

The residual brine of the Cerro Prieto Geothermal Field (CPGF) is disposed in an evaporation pond. The seepage of this pond has contaminated the water and agricultural soil around it. The contamination of the groundwater towards the southwest by the evaporation pond, in the direction of the regional flow, has been shown before. Hydrogeochemical modeling (PHREEQCI) and Schoeller and Piper diagrams have been used in this work to show that the chemical composition of the groundwater in villages neighboring CPGF is the product of mixing between irrigation water from the Colorado River and brine from the evaporation pond. The high potassium concentration in the water and the relative increase in concentration of sodium and chlorides along the flow path as well as the hydrogeochemical models for this system explain this mixing process. This work will allow proposing new managing techniques to avoid the presence of the residual brine in the groundwater of agricultural lands. © 2010 Springer Science+Business Media B.V.


Ortega-Nieblas M.M.,University of Sonora | Robles-Burgueno M.R.,Research Center En Alimentacion sarrollo Ac | Vazquez-Moreno L.,Research Center En Alimentacion sarrollo Ac | Cortez-Mondaca E.,INIFAP Campo Experimental Valle Del Fuerte | And 3 more authors.
Southwestern Entomologist | Year: 2014

Zabrotes subfasciatus (Coleoptera: Bruchidae), is one of the most destructive species with greater abundance and distribution in stored beans Phaseolus vulgaris L., causing great economic losses in Sonora, Mexico. Control of this pest has been based mainly on the use of broad-spectrum synthetic insecticides. Their intensive application has caused insect resistance, environmental pollution and impact on human health, therefore increasing research to control it has the aim of developing safe alternatives with potential to replace synthetic insecticides, while not being harmful. We studied the toxic effect and persistence of the essential oil (EO) of Lippia palmeri (Watson) (Verbenaceae) on Zabrotes subfasciatus (Boheman) in stored beans. The EO was compared between plant leaves collected from two locations: Puerto of Oregano (POEO) and Alamos Sonora (AEO). Beans were treated with 0, 0.135, 1.35, 4.0, and 8.0 μl/g of EO and infested with Z. subfasciatus. Mortality, oviposition, insect emergence, and percentage of damaged grain were assessed for 4 months. The POEO caused greater mortality, and both EOs showed ovicidal effects that persisted for 2 months with the 1.35 μl/g dose. Moreover, lethal effects after 3 months of storage at 4 and 8 μl/g were observed, either in insect emergence or damaged grain. This seems to be caused by the persistence of the essential oils carvacrol, thymol, and p-cymene in the Lippia palmeri samples.


PubMed | Institute Ciencias Agricolas
Type: Journal Article | Journal: Journal of animal physiology and animal nutrition | Year: 2013

An experiment was conducted to evaluate the effect of excess levels of Leu and Lys on the expression of b(0,+) and CAT-1 mRNA in jejunum, liver and the muscles Longissimus dorsi (LDM) and Semitendinosus (STM). Twenty pigs with an average initial BW of 16.41.7kg were used in a Randomized Complete Block. Dietary treatments (T) were as follows: T1, basal diet; T2, basal plus 3.5g l-Lys/kg diet; T3, basal plus 1.5g l-Leu/kg diet; T4, basal plus 3.5g l-Lys plus 1.5g l-Leu/kg diet. Diets in T1 and T3 met 100% the requirement of Lys for pigs within the 10 to 20kg body weight range; diets in T2 and T4 contained 35% excess of Lys. Also, diets in T1 and T2 supplied 104%, whereas diets in T3 and T4 supplied 116% the requirement of Leu. The expression of b(0,+) in jejunum was reduced (p=0.002) because of the supplementation of l-Leu, but l-Lys supplementation had no effect (p=0.738). In contrast, the expression of b(0,+) in STM (p=0.012) and liver (p=0.095) was reduced by the high level of Lys, but Leu had no effect (p>0.100). CAT-1 expression in STM increased by high Lys (p=0.023) and Leu (p=0.007) levels. In liver, the expression of CAT-1 substantially increased (p=0.001) because of Lys. In conclusion, excess levels of dietary Lys and Leu affect the expression of cationic amino acid transporters, and this effect varies depending on the studied tissue.


PubMed | Sonora Institute of Technology, Institute Ciencias Agricolas and Autonomous University of Baja California
Type: Journal Article | Journal: Animal science journal = Nihon chikusan Gakkaiho | Year: 2016

To investigate the effects of progesterone supplementation at two different times on serum progesterone (P4 ) concentration, conception rate and resynchronization of cooled Holstein heifers in summer, 90 heifers were randomly assigned to two groups: (i) heifers subjected to TAI (timed artificial insemination) and progesterone supplementation from days 4 to 14 after TAI (S1; n=45); and (ii) heifers under the same TAI protocol as S1 and progesterone supplementation from days 17 to 22 after TAI (S2 ; n=45). The groups S1 and S2 were cooled 10days before and 21days after TAI. Respiratory rate, body surface temperature, vaginal temperature and rectal temperature recorded during the experiment were not different (P>0.05) between S1 and S2 groups. Progesterone concentration was not different (P>0.05) in S1 compared to S2 . The conception rates on days 30 and 55 were similar between groups (P>0.05). Progesterone supplementation did not increase either conception rate or concentrations of P4 in heifers during the summer. Heifers not pregnant to first service in the group S2 were resynchronized (77.7%) for a second breeding.


PubMed | Institute Ciencias Agricolas
Type: Journal Article | Journal: Archives of animal nutrition | Year: 2012

The absorption of lysine is facilitated by leucine, but there is no information regarding the effect of crude protein, lysine and leucine levels on the expression of cationic amino acid transporters in pigs. Therefore, an experiment was conducted with 20 pigs (14.9 +/- 0.62 kg initial body weight) to evaluate the effect of two protein levels, and the content of lysine, threonine, methionine and leucine in low crude protein diets on the expression of b(0,+) and CAT-1 mRNA in jejunum, Longissimus dorsi and Semitendinosus muscles and serum concentration of amino acids. Treatments were as follows: (i) wheat-soybean meal diet, 20% crude protein (Control); (ii) wheat diet deficient in lysine, threonine and methionine (Basal diet); (iii) Basal diet plus 0.70% L-lysine, 0.27% L-threonine, 0.10% DL-methionine (Diet LTM); (iv) Diet LTM plus 0.80% L-leucine (Diet LTM + Leu). Despite the Basal diet, all diets were formulated to meet the requirements of lysine, threonine and methionine; Diet LTM + Leu supplied 60% excess of leucine. The addition of lysine, threonine and methionine in Diet LTM increased the expression of b(0,+) in jejunum and CAT-1 in the Semitendinosus and Longissiums muscles and decreased CAT-1 in jejunum; the serum concentration of lysine was also increased (p < 0.01). Further addition of L-leucine (Diet LTM + Leu) decreased the b(0,+) expression in jejunum and CAT-1 in the Longissimus dorsi muscle (p < 0.05), increased the serum concentration ofleucine and arginine and decreased the concentration of isoleucine (p < 0.05). Pigs fed the Control diet expressed less b(0,+) in jejunum, and CAT-1 in the Semitendinosus and Longissiums muscles expressed more CAT-1 in jejunum (p < 0.05) and had lower serum concentration ofisoleucine, leucine and valine (p < 0.05), but higher lysine concentrations (p < 0.01) than those fed Diet LTM. These results indicated that both, the level and the source of dietary amino acids, affect the expression of cationic amino acid transporters in pigs fed wheat-based diets.

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