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Diez-Pascual A.M.,University of Alcala | Shuttleworth P.S.,Institute Ciencia Y Tecnologia Of Polimeros Consejo Superior Of Investigaciones Cientificas
Materials | Year: 2014

This review deals with the layer-by-layer (LbL) assembly of polyelectrolyte multilayers of biopolymers, polypeptides (i.e., poly-L-lysine/poly-L-glutamic acid) and polysaccharides (i.e., chitosan/dextran sulphate/sodium alginate), onto thermoand/or pH-responsive micro- and nano-gels such as those based on synthetic poly(N-isopropylacrylamide) (PNIPAM) and poly(acrylic acid) (PAA) or biodegradable hyaluronic acid (HA) and dextran-hydroxyethyl methacrylate (DEX-HEMA). The synthesis of the ensembles and their characterization by way of various techniques is described. The morphology, hydrodynamic size, surface charge density, bilayer thickness, stability over time and mechanical properties of the systems are discussed. Further, the mechanisms of interaction between biopolymers and gels are analysed. Results demonstrate that the structure and properties of biocompatible multilayer films can be finely tuned by confinement onto stimuli-responsive gels, which thus provides new perspectives for biomedical applications, particularly in the controlled release of biomolecules, bio-sensors, gene delivery, tissue engineering and storage. © 2014 by the authors. Source


Garcia-Garcia J.M.,Institute Ciencia Y Tecnologia Of Polimeros Consejo Superior Of Investigaciones Cientificas | Quijada-Garrido I.,Institute Ciencia Y Tecnologia Of Polimeros Consejo Superior Of Investigaciones Cientificas | Lopez L.,Hospital Universitario Fundacion Of Alcorcon | Paris R.,Institute Ciencia Y Tecnologia Of Polimeros Consejo Superior Of Investigaciones Cientificas | And 3 more authors.
Materials Science and Engineering C | Year: 2013

Biocompatible and biodegradable poly(3-hydroxybutyrate-co-3- hydroxyhexanoate) [P(HB-co-HHx)] substrates were modified to improve the attachment of porcine urothelial cell culture. The pristine copolymer exhibits excellent mechanical properties to replace the bladder tissue, but its surface lacks chemical functionalities to interact with cells. Thus, wet chemical treatments based on NaOH and ethylenediamine in aqueous [ED(aq)] and isopropanol [ED(isoOH)] media to functionalize the P(HB-co-HHx) films surfaces were compared. Among these treatments, short ED(aq) treatment was able to decrease the hydrophobicity, rendering a surface with amino groups and without a significant alteration of the mechanical properties. Furthermore, to enhance the interaction with urothelial cells, laminin derived YIGSR sequence was covalently bound to these amino functionalized substrates. The focal attachment was clearly improved with this last treatment, comparing with those results found with the unmodified and first-step functionalized P(HB-co-HHx). © 2012 Elsevier B.V. Source

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