Institute Ciencia Y Tecnologia Of Alimentos Icta

Córdoba, Argentina

Institute Ciencia Y Tecnologia Of Alimentos Icta

Córdoba, Argentina
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Dominchin M.F.,National University of Cordoba | Palme R.,University of Veterinary Medicine Vienna | Marin R.H.,National University of Cordoba | Busso J.M.,National University of Cordoba | Busso J.M.,Institute Ciencia Y Tecnologia Of Alimentos Icta
Journal of Ornithology | Year: 2017

This study evaluates whether short daily male-conspecific visits influence the dynamics of endocrine testicular and adrenal photo-responsiveness. A group of male Japanese Quail was exposed to short photoperiods and another group to long photoperiods. The quail exposed to short photoperiods were classified as responsive (SD-R) or non-responsive (SD-NR), depending on whether or not they lost their reproductive condition after 5 weeks of short days (SD). Individuals kept on a long photoperiod were used as controls (LD). After photoperiodic classification and during four consecutive days, individually-caged SD-R, SD-NR and LD resident male quail received 5-min visits from photo-stimulated (and therefore sexually active) male quail (social treatment). Cloacal gland volume (CGV), and androgen and corticosterone metabolites (AM and CM, respectively) in droppings were measured bi-weekly and evaluated by repeated measures ANOVA over a 6-week period. Immediately after photoperiodic classification and before social treatment, CGV, AM and CM values showed a SD-R < SD-NR < LD pattern (P < 0.001). Along SD exposure, SD-NR quail showed spontaneous cloacal gland recovery and AM and CM concentrations were found to be similar to those of the LD quail. At the end of the study, the SD-R group had still not recovered LD control values. Although the social treatment induced an overall main effect on AM (P < 0.02) and CM concentrations (P < 0.009), these changes were not related to a particular photoperiodic treatment. These findings provide a novel insight into the social modulation of reproductive and adrenocortical responses, particularly during transition from the photosensitive state to photorefractoriness. © 2017, Dt. Ornithologen-Gesellschaft e.V.


Vera F.,University of the Sea | Zenuto R.R.,University of the Sea | Zenuto R.R.,CONICET | Antenucci C.D.,University of the Sea | And 5 more authors.
Journal of Experimental Zoology Part A: Ecological Genetics and Physiology | Year: 2011

We validated the Coat-a-Count ® radioimmunoassay (RIA) kit for measuring testosterone in plasma samples of the subterranean rodent Ctenomys talarum and evaluated testosterone levels in free-living and captive individuals. The performance of the assay was evaluated by the assessment of parallelism, accuracy and precision. Moreover, the high specificity of the assay antibody was confirmed by high-pressure liquid chromatography with ultraviolet detector, followed by electrospray ionization tandem mass spectrometry. Results indicated that plasma samples have to be treated with heat and diluted before the RIA for the optimization of the assay. Plasma testosterone concentrations in free-living animals were outstandingly elevated (up to 329ng/mL), which are among the highest ever reported for mammals. On the other hand, captivity produced a 14-fold decrease in plasma testosterone concentrations, emphasizing that very significant changes in the endocrine milieu may occur in wild animals kept under laboratory conditions. Our results place tuco-tucos as an interesting model for the study of androgen regulation in mammals, suggesting that target tissues may have low sensitivity to the testosterone signal and agree with a scenario of elevated levels of sex hormone-binding globulin in plasma. © 2011 Wiley Periodicals, Inc.

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