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Dine G.,Institute Biotechnologique Of Troyes | Dine G.,Ecole Centrale Paris | Genty V.,Institute Biotechnologique Of Troyes | Rehn Y.,Institute Biotechnologique Of Troyes | And 2 more authors.
Bio Tribune Magazine | Year: 2010

The global study of primary hemostasis process is regularly performed in case of bleeding syndrome. The study of the role of platelets in the pathogenesis of bleeding syndrome is frequently necessary but also in case of ischemic vascular disease. Classical platelet aggregation tests and cytometry analysis are not suited for routine used. Specialized assays will be planned in expert laboratories. Today several techniques are in use to measure platelet aggregation. The monitoring of antiplatelet treatments is up to date and requires reliable platelet function tests. The recent development of rapid whole blood point of care platelet function assays allows the monitoring of platelet inhibition in much larger populations than previously possible. The results are well correlated with turbidimetric aggregometry and receptor binding assays. Ready to use apparatus at any time without any previous preparation are useful in the investigation of haemostatic disorders and ischemic vascular diseases. But platelet aggregometry and cytometry assays remain gold standard methods in case of difficult diagnosis. © Springer 2009.

Dine G.,Institute Biotechnologique Of Troyes | Dine G.,Ecole Centrale Paris | Fumagalli G.,Institute Biotechnologique Of Troyes | van Lierde F.,Institute Biotechnologique Of Troyes | Genty V.,Institute Biotechnologique Of Troyes
Bio Tribune Magazine | Year: 2010

The human erythron is responsible for oxygen transport and includes the erythroid marrow, circulating red cells, the réticulo-endothelial system and the erythropoietin producing cells in the kidney. These renal cells have a mechanism for oxygen sensing. In normoxia oxygen activates the PH factor which hydroxylate a subunit of the transcription factor HIF. In hypoxia this subunit HIFα together with the subunit HIFβ activate EPO gene. Increased EPO binds to the receptor on erythroid progenitors and leads to increased red cells production as an appropriate response to hypoxia. Alterations in the oxygen sensing pathway and abnormalities of the EPO signaling pathway lead to primary and secondary erythrocytosis. Immature erythroid cells are the major consumers of iron in the body. Elevated erythropoesis increases intestinal iron absorption and stocked iron mobilization. Considering its potential toxicity and low bioavailability, iron metabolism is a tightly regulated process in humans. Control mechanisms maintain iron homeostasis by coordinately regulating iron absorption, iron recycling and mobilization of stored iron. Dietary iron absorption is locally regulated by HIF and IRPs in enterocytes and systematically by hepatic hepcidin, the central iron regulatory hormone. Hepcidin not only controls the rate of iron absorption but also determines iron mobilization from stocks through modulating ferroportin protein. © 2010 Springer Verlag France.

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