Carrera J.,Institute Biologa Molecular Y Celular Of Plantas |
Carrera J.,Genopole |
Fernandez del Carmen A.,Institute Biologa Molecular Y Celular Of Plantas |
Fernandez-Munoz R.,Institute Hortofruticultura Subtropical y Mediterranea La Mayora IHSM UMA CSIC |
And 6 more authors.
PLoS Computational Biology | Year: 2012
Considering cells as biofactories, we aimed to optimize its internal processes by using the same engineering principles that large industries are implementing nowadays: lean manufacturing. We have applied reverse engineering computational methods to transcriptomic, metabolomic and phenomic data obtained from a collection of tomato recombinant inbreed lines to formulate a kinetic and constraint-based model that efficiently describes the cellular metabolism from expression of a minimal core of genes. Based on predicted metabolic profiles, a close association with agronomic and organoleptic properties of the ripe fruit was revealed with high statistical confidence. Inspired in a synthetic biology approach, the model was used for exploring the landscape of all possible local transcriptional changes with the aim of engineering tomato fruits with fine-tuned biotechnological properties. The method was validated by the ability of the proposed genomes, engineered for modified desired agronomic traits, to recapitulate experimental correlations between associated metabolites. © 2012 Carrera et al.