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Villanueva de Castellón, Spain

Torres-Nunez E.,CSIC - Institute of Marine Research | Suarez-Bregua P.,CSIC - Institute of Marine Research | Cal L.,CSIC - Institute of Marine Research | Cal R.,Spanish Institute of Oceanography | And 2 more authors.
Gene | Year: 2015

SPARC/osteonectin is a multifunctional matricellular glycoprotein, which is expressed in embryonic and adult tissues that undergo active proliferation and dynamic morphogenesis. Recent studies indicate that Sparc expression appears early in development, although its function and regulation during development are largely unknown. In this report, we describe the isolation, characterization, post-embryonic developmental expression and environmental thermal regulation of sparc in turbot. The full-length turbot sparc cDNA contains 930. bp and encodes a protein of 310 amino acids, which shares 77, 75 and 80% identity with human, frog and zebrafish, respectively. Results of whole-mount in situ hybridization reveal a dynamic expression profile during post-embryonic turbot development. Sparc is expressed differentially in the cranioencephalic region; mainly in jaws, branchial arches, fin folds and rays of caudal, dorsal and anal fins. Furthermore, ontogenetic studies demonstrated that Sparc gene expression is dynamically regulated during post-embryonic turbot development, with high expression during stage-specific post-embryonic remodeling. Additionally, the effect of thermal environmental conditions on turbot development and on ontogenetic sparc expression was evaluated. © 2015 Elsevier B.V.

Rodrigues C.M.,University of Porto | Bio A.,University of Porto | Amat F.,Institute Acuicultura Of Torre Of La Sal Iats Csic | Vieira N.,University of Porto
Saline Systems | Year: 2011

Solar salinas are man-made systems exploited for the extraction of salt, by solar and wind evaporation of seawater. Salt production achieved by traditional methods is associated with landscapes and environmental and patrimonial values generated throughout history. Since the mid-twentieth century, this activity has been facing a marked decline in Portugal, with most salinas either abandoned or subjected to destruction, making it necessary to find a strategy to reverse this trend.It is, however, possible to generate revenue from salinas at several levels, not merely in terms of good quality salt production, but also by obtaining other products that can be commercialized, or by exploring their potential for tourism, and as research facilities, among others. Furthermore, with an adequate management, biodiversity can be restored to abandoned salinas, which constitute important feeding and breeding grounds for resident and migratory aquatic birds, many of which are protected by European Community Directives.The aims of this manuscript are to present a brief overview on the current state of sea salt exploitation in Portugal and to stress the importance of recovering these salinas for the conservation of this particular environment, for the regional economy, the scientific community and the general public. The Aveiro salina complex is presented in detail, to exemplify salina structure and functioning, as well as current problems and potential solutions for artisanal salinas. © 2011 Rodrigues et al; licensee BioMed Central Ltd.

Constenla M.,Autonomous University of Barcelona | Padros F.,Autonomous University of Barcelona | Palenzuela O.,Institute Acuicultura Of Torre Of La Sal Iats Csic
Journal of Fish Diseases | Year: 2014

A new amoeba species pathogenic for Senegalese sole is described based on ultrastructural analysis and SSU rDNA phylogenetic inference. The parasite presents round to ovoid trophozoites (<5 μm) with a high degree of intracellular simplification. No mitochondria were observed, but mitosome-like organelles were present. No cysts could be detected. Phylogenetic analysis confirmed the Senegalese sole parasite as an amitochondriate Archamoeba related to Endolimax nana and Iodamoeba spp., and we tentatively describe it as a new species in the genus Endolimax, Endolimax piscium. However, the genetic distance with E. nana is quite large, with only 60% pairwise identity between both SSU rDNA genotypes. Although the overall topology of the Archamoebae cladograms containing E. piscium was consistent, the support for the branching of Endolimax spp. relative to its closest neighbours was variable, being higher with distance or parsimony-based inference methods than with ML or Bayesian trees. The use of stringent alignment sampling masks also caused instability and reduced support for some branches, including the monophyly of Endolimax spp. in the most conservative data sets. The characterization of other Archamoebae parasitizing fish could help to clarify the status of E. piscium and to interpret the large genetic distance observed between Endolimax species. © 2013 John Wiley & Sons Ltd.

Pinto P.M.,University of Porto | Hontoria F.,Institute Acuicultura Of Torre Of La Sal Iats Csic | Vieira N.,University of Porto | Bio A.,University of Porto
Estuarine, Coastal and Shelf Science | Year: 2014

There is widespread interest in the conservation of native Artemia biodiversity. In Portugal, only two known populations of native Artemia remain: one in the Rio Maior salina, the other in the Aveiro salina complex, both of the diploid Artemia parthenogenetica species. All other Portuguese hypersaline environments where Artemia can be found have been invaded by Artemia franciscana, which has eradicated the native strains. Invasiveness and resilience of, respectively, exotic and indigenous species are thought to depend on strain-specific traits and adaptation to local conditions. This work evaluates the reproductive performance of the two Portuguese native strains and the invasive species exposed to different salinities, temperatures, photoperiods and food supplies. Reproduction periods, quantity and quality of offspring varied significantly, depending on both the Artemia strain and environmental conditions. A.parthenogenetica from Rio Maior reproduced better than A.franciscana at high salinity (150) and low food supply, which may reflect an adaptation to its biotope that aids its resistance to invasion. But A.parthenogenetica form Aveiro performed much worse than its invasive competitor, under most of the conditions tested. It is unlikely that A.franciscana has not been introduced in this salina by chance alone. Other biological traits of the local A.parthenogenetica or adaptation to unstudied local factors (e.g. pollution) are probably responsible for this strain's survival. Further knowledge on specific local conditions and trait-specific tolerances to biotic and abiotic conditions are needed to understand (non-)invasion patterns and preserve the remaining native populations. © 2014 Elsevier Ltd.

Pinto P.M.,University of Porto | Bio A.,University of Porto | Hontoria F.,Institute Acuicultura Of Torre Of La Sal Iats Csic | Almeida V.,University of Porto | Vieira N.,University of Porto
Journal of Experimental Marine Biology and Ecology | Year: 2013

There are currently only two places in Portugal were native Artemia parthenogenetica can still be found. All other known populations have been eradicated by the invasive species Artemia franciscana, which has caused great losses of Artemia biodiversity in the Mediterranean region. The diploid strains found at the Portuguese salines are therefore of high conservation value. This study aims to assess the survival of these native A. parthenogenetica strains and of A. franciscana under a variety of environmental conditions. The effects of water temperature and salinity, of photoperiod and food supply (shortage) were studied in an experimental setup.The Portuguese parthenogenetic Artemia populations showed great variability in their physiological response to different abiotic conditions, suggesting possible local adaptations in response to different selective pressures experienced. For most of the conditions studied A. franciscana outcompeted the Artemia strain from Aveiro, whereas the strain from Rio Maior was more resistant than the A. franciscana under conditions that were similar to its local habitat. Strain-specific resistance to chemical conditions, related to pollution, are appointed as a potential cause why A. franciscana did not successfully invade Aveiro saline. The saline of Rio Maior has possibly not yet been invaded due to the fitness of its local Artemia strain in combination with its inland location. © 2012 Elsevier B.V.

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