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Aldeadávila de la Ribera, Spain

Bromberg L.,Massachusetts Institute of Technology | Bromberg D.J.,Massachusetts Institute of Technology | Hatton T.A.,Massachusetts Institute of Technology | Bandin I.,Institute Acuicultura | And 2 more authors.
Langmuir | Year: 2012

Polycationic superparamagnetic nanoparticles (∼150-250 nm) were evaluated as virucidal agents. The particles possess a core-shell structure, with cores consisting of magnetite clusters and shells of functional silica covalently bound to poly(hexamethylene biguanide) (PHMBG), polyethyleneimine (PEI), or PEI terminated with aziridine moieties. Aziridine was conjugated to the PEI shell through cationic ring-opening polymerization. The nanometric core-shell particles functionalized with biguanide or aziridine moieties are able to bind and inactivate bacteriophage MS2, herpes simplex virus HSV-1, nonenveloped infectious pancreatic necrosis virus (IPNV), and enveloped viral hemorrhagic septicaemia virus (VHSV). The virus-particle complexes can be efficiently removed from the aqueous milieu by simple magnetocollection. © 2012 American Chemical Society. Source

Diana Bravo T.,University of the Plains | Cruz-Casallas P.,Institute Acuicultura | Julieta Ochoa A.,University of the Plains
Revista MVZ Cordoba | Year: 2010

Objective: Describe and classify neoplastic diseases diagnosed at the Veterinary Pathology Laboratory at the University of the Llanos, between 2004 and 2007. Materials and methods: As a source of information was used database of medical records and autopsy examinations of the Veterinary Clinic of the University of the Llanos. The samples came from specimens obtained by biopsies and tumors found during necropsy. We analyzed 322 clinical cases, of which 113 were for cancer diseases (35%).Results: In general, the most affected species was the dog (93 of 113 cases), mixed races being the most affected (p <0.05) and the organic system more injured skin, with 68 cases (60.2%), followed by the gland breast with 13 cases (11.5%). The highest incidence of neoplasms were: squamous cell carcinoma (15 cases), trichoepithelioma (11 cases) and canine mastocytoma III (10 cases). There was a positive relationship between age and presentation of tumors (p <0.01), sex was not a determining factor for the presentation of the disease (p> 0.05). Conclusions: The skin tumors were the most impact, perhaps because of the ease of diagnosis during the clinical consultation, compared with other neoplasms that affect deeper organs and tissues. Source

Alvarado M.V.,Institute Acuicultura | Carrillo M.,Institute Acuicultura | Felip A.,Institute Acuicultura
General and Comparative Endocrinology | Year: 2013

Kisspeptins play a critical role in the control of hypothalamic-gonadotropic function and puberty onset in mammals. Studies in fish have all supported the hypothesis that they might play similar roles in the reproduction of this animal group, however, their physiological relevance in the occurrence of key reproductive events still remains to be determined. This study examines the relative mRNA expression profiles of the duplicate kisspeptin system (kiss1, kiss2, gpr54-1b, and gpr54-2b) in the hypothalamus and pituitary of adult male and female sea bass (Dicentrarchus labrax L.) during different gonadal stages using qRT-PCR. We also report the changes in the expression levels of gnrh-1, gnrhr-II-1a, fshβ, and lhβ and the relationships observed between both kisspeptin and GnRH systems. Our data show clear sex differences in the dynamics of kisspeptin and kisspeptin receptor gene expression in the hypothalamus of sea bass during gonadal development. Overall, all four kisspeptin system genes increased either before or during the advanced stages of oogenesis and declined during atresia, exhibiting profiles that are identical to those observed for gnrhr-II-1a, fshβ, lhβ, and the gonadosomatic index (GSI). While the situation was not as clear in males, the high kiss2 expression levels observed in the hypothalamus during mid recrudescence suggest that it might be playing a role in the neuroendocrine signaling that regulates germ cell proliferation at the testicular level. In this sense, the proposed role attributed to kisspeptins as key factors in the onset of reproduction in fish receives an additional support from the data obtained in the present work. Nevertheless, further research is required to clarify their precise role in sea bass. © 2013 Elsevier Inc. Source

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