Jiang L.,Shanghai Academy of Agriculture science |
Jiang L.,Inspection and Test Center for Environmental Safety of Crops of Shanghai |
Liu H.,Shanghai Academy of Agriculture science |
Liu H.,Inspection and Test Center for Environmental Safety of Crops of Shanghai |
And 14 more authors.
Journal of Medicinal Plants Research | Year: 2010
Artemisinin, a sesquiterpene isolated from Artemsisia annua L. (Chinese wormwood) is an increasingly popular drug against malaria. In this study, three transgenic A. annua varieties with increased artemisinin content were evaluated in an environmental release trial. First, because there were risks concerning genetically modified plants, the 3 transgenic varieties were compared with that of wild-type plants in regard to agronomic traits, viability and expressed material. Second, stress tolerances (salt, drought and herbicide) were evaluated under experimental conditions. The results indicated significant differences between the 3 transgenic and wild-type plants in some agronomic traits (as in plant height, crown width, stem diameter, 1000-seeds weight), and no significant differences in leaf shape. Also, the viability (germination rate, transplant survival and seeds wintering ability) from both transgenic and control plants were similar. Moreover, it also was determined that the 3 transgenic and control plants had similar stress tolerances (salt tolerance, drought tolerance, herbicide tolerance). The expression of target genes in transgenic varieties was either enhanced or suppressed. Thus, the artemisinin content was increased in transgenic varieties as expected. © 2010 Academic Journals.
Liu H.,Shanghai Academy of Agricultural science |
Jiang L.,Shanghai Academy of Agricultural science |
Tan F.,Shanghai Academy of Agricultural science |
Zhao X.,Inspection and Test Center for Environmental Safety of Crops of Shanghai |
And 11 more authors.
Journal of AOAC International | Year: 2013
Artemisia annua L. is a popular medical plant used against malaria. Transgenic A. annua plants have been developed to increase the artemisinin content using genetic engineering. To develop a reliable PCR method of detecting transgenic A. annua, one Artemisia specific gene, GEL4, was selected and validated as suitable. Qualitative PCR methods were assayed with 16 different A. annua ecotypes and four Artemisia plants, and identical amplified products were obtained. No amplified products were observed when genomic DNA samples were extracted from 15 different plant species. An LOD of 0.05 ng of A. annua DNA was also determined. Furthermore, the results of a Southern blot method showed that the GEL4 gene was in three copies of the six A. annua ecotypes tested. To determine whether there was intraspecific variability in the GEL4 region between different A. annua ecotypes, the GEL4 region corresponding to the 10 A. annua ecotypes was cloned and sequenced. Alignment of the obtained sequences presented a highly conserved amplification product region in 10 A. annua ecotypes. Therefore, it is concluded that the GEL4 gene can be used as an endogenous reference gene of A. annua, and that the qualitative PCR system was reliable for the detection of transgenic A. annua.