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Perez A.,National Polytechnic Institute of Mexico | Santos Cuevas C.L.,Instituto Nacional de Investigaciones Nucleares | Chairez I.,National Polytechnic Institute of Mexico | Poznyak T.,National Polytechnic Institute of Mexico | And 3 more authors.
Anti-Cancer Agents in Medicinal Chemistry | Year: 2015

C6 rat glioma cells are one of the most aggressive carcinogenic tumors, due to its high mortality rate in human beings and animals. The current treatment for this illness includes surgery, radio and chemotherapy, showing relapse in patients treated with those therapies. Since the ozone was found to be an effective bioreactive to inhibit growth of several carcinoma cells in vitro and in vivo. In this research, therapeutic peritoneum insufflation of ozone/oxygen dissolved in the physiological solution of NaCl 0.9% was dosed for fifteen days on different female mice groups in an advanced stage of C6 tumor (n=6). The first of them was the control group which had no treatment, the second group was dosage with oxygen every second day, the third group was dosed with ozone every second day, and finally the fourth group was dosed with ozone dissolved every fifth day. The size of the tumor was higher in both groups dosage by ozone, nevertheless tumor activity measured by microPET was 98% less in the fourth group compared with the control group. That result proves that ozone provokes an increase in the tumor volume even though the decrease of the cell activity. Those results were confirmed by the quantification of hydroperoxides, total cholesterol and total triglycerides © 2015 Bentham Science Publishers.


Perez A.,National Polytechnic Institute of Mexico | Santos Cuevas C.L.,Instituto Nacional de Investigaciones Nucleares | Chairez I.,National Polytechnic Institute of Mexico | Poznyak T.,National Polytechnic Institute of Mexico | And 3 more authors.
Analytical Methods | Year: 2014

Due to its chemical properties, ozone gas has been used in the medical field for the treatment of many diseases. In general, all therapies based on ozone reported regulatory clinical effects. However, ozone dosage has not been clearly defined and connected with the specific stage of the illness evolution. The double bond index has been used to identify or characterize the effects of specific therapies on patients. The aim of this article was explore the possibility of using the DB index as a simple and fast biochemical test to characterize the effects of ozone therapy for cancer. In this study, this claim was confirmed with an in vitro and in vivo test. The proliferation analysis of C6 rat glioma cells was correlated with the DB-index variation. The in vivo test used athymic mice with inducted tumors of the same cell line (C6). The tumor's volume and its activity, the cholesterol/triglyceride variations and the hydroperoxide quantification were correlated with the DB index variations in plasma, erythrocytes and tissues. In all cases, there was a specific and plausible relationship between the DB index behavior in the clinical and imaging results obtained by micro PET studies. © the Partner Organisations 2014.

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