Lisbon, Portugal
Lisbon, Portugal

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Selmi S.,Institute National Des Science Et Technologies Of La Mer | Batista I.,INRB IPIMAR | Sadok S.,Institute National Des Science Et Technologies Of La Mer | Bandarra N.M.,INRB IPIMAR | Nunes M.L.,INRB IPIMAR
Journal of Food Process Engineering | Year: 2010

Washed sardine mince was prepared according to the following scheme: (1) evisceration and beheading by hand; (2) mince preparation in a deboner; (3) first washing in a water solution with 0.2% NaHCO3 and 0.15% NaCl; second washing in water; and (4) third washing in 0.25% NaCl. A significant increase (P < 0.05) of the peroxide value occurred during the mince preparation process. However, no significant difference in conjugated diene and thiobarbituric acid index was recorded. The washing treatments led to a release of lipids, which were mainly constituted by triacylglycerols rich in n-3 fatty acids. A significant increase in the polar lipids fraction (especially phosphatidylcholine) was recorded in mince sardine. Eicosapentaenoic acid level of neutral lipid decline throughout the mincing process, but the docosahexaenoic acid level showed a significant increase. Minced sardine obtained in these conditions could be considered a good source of amino acids and n-3 fatty acids. © 2009 Wiley Periodicals, Inc.


Selmi S.,Institute National Des Science Et Technologies Of La Mer | Limam Z.,Institute National Des Science Et Technologies Of La Mer | Batista I.,INRB IPIMAR | Bandarra N.M.,INRB IPIMAR | Nunes M.L.,INRB IPIMAR
International Journal of Refrigeration | Year: 2011

Sardine oil released during sardine mince preparation showed good quality with a low hydroperoxide content, excellent color and high content (19.23%) of eicosapentaenoic acid. Recovered sardine oil was stored at two different temperatures (+4 °C and +35 °C) for 28 days with or without the addition of α-tocopherol (50 and 100 ppm). Peroxide values and thiobarbituric acid reactive substances of control sardine oil significantly increased to reach 29.9 meq O2/kg oil and 46.48 mg MA/kg during storage at +35 °C, but the increase was considerably less (4.36 meq O2/kg oil and 13.21 mg MA/kg oil respectively) in oil stored at +4 °C. A slight increase (1.5%) in the free fatty acid content was recorded in oil stored at 35 °C. A significant decrease of polyunsaturated fatty acids was recorded after storage, particularly in oil stored at 35 °C, while higher percentages of saturated and monounsaturated fatty acids were observed. Storage at +4 °C combined with addition of α-tocopherol (100 ppm) had a beneficial effect on sardine oil stability. © 2011 Elsevier Ltd and IIR. All rights reserved.


Pan M.,INRB IPIMAR | Pierce G.J.,University of Aberdeen | Cunningham C.O.,INRB IPIMAR | Hay S.J.,INRB IPIMAR
Journal of the Marine Biological Association of the United Kingdom | Year: 2011

The patterns of decapod larvae occurrence and abundance were studied from weekly time-series data of 8 years from Stonehaven (north-east Scotland) and 4 years and 8 months from Loch Ewe (north-west Scotland). The annual cycle observed was similar in the two locations and characterized by abundance peaks, the first in spring and another in the summer, extending into autumn. During the coldest months (December to February) decapod larvae were virtually absent in the plankton. Differences in abundance and occurrence of decapod larvae between locations and the influence of temperature, salinity and chlorophyll-a in the patterns observed, were analysed by generalized least-square functions. The results showed significant differences in the abundance of decapod larvae between locations, with higher larval abundances and an earlier appearance in the plankton in Loch Ewe (west coast). In Stonehaven (east coast), from 2003 onwards, a general increasing trend in the abundance of decapod larvae was observed, related to the increasing temperatures recorded at that site. The data demonstrate the high variability of decapod larval abundance on an annual basis and the high importance of temperature and chlorophyll-a to the occurrence and abundance of decapod larvae. © 2011 Marine Biological Association of the United Kingdom.


Bandarra N.M.,INRB IPIMAR | Rema P.,UTAD | Rema P.,University of Porto | Batista I.,INRB IPIMAR | And 4 more authors.
European Journal of Lipid Science and Technology | Year: 2011

The current study was conducted to evaluate the effects of various dietary n-3/n-6 ratio on growth and lipid metabolism in gilthead seabream (Sparus aurata) juveniles. The fish were fed ad libitum on three isoproteic (52%), isolipidic (14%), and isoenergetic diets (21MJ/kg), formulated to contain three different n-3/n-6 ratios: a high (3.8), medium (2.5), or low (0.9) ratio. Fish grew from 9.5±0.1g to 54.2±0.7g in 12 weeks. Growth rate (2.2% BW/day), voluntary feed intake (2.4±0.1% BM/day), feed conversion ratio (1.38±0.05), and net protein utilization (27.5±1.0%) did not vary among treatments. In contrast, body lipid content and lipid deposition efficiency were significantly reduced in fish fed low n-3/n-6 ratio. Fish fed the highest n-3/n-6 ratio showed the lowest hepatic glucose-6-phosphate dehydrogenase (G6PD) activity (p=0.02). The n-3/n-6 ratio decreased, while DHA/eicosapentaenoic acid (EPA) ratio increased significantly with decreasing dietary n-3/n-6 content. Among n-3 PUFA, the DHA and, particularly, the docosapentaenoic acid (DPA) showed the highest deposition rate both in muscle and liver. The lower deposition rate (<1) of linoleic acid (LA) and linolenic acid (LNA) suggests that a reduction in dietary n-3/n-6 ratio may stimulate their transport to the intermediary metabolism for energy production. © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

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