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Adelaide, Australia
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Frederiksen P.D.,University of Aarhus | Hammershoj M.,University of Aarhus | Bakman M.,Arla Foods | Andersen P.N.,Arla Foods | And 3 more authors.
Dairy Science and Technology | Year: 2011

The paper describes a study on the variation of coagulation properties in individual cow's milk measured by a novel free oscillation rheometry-based method. The work is part of a study on the occurrence and underlying causes of poorly and even non-coagulating (NC) milk in Denmark, and based on that, the paper reports on the frequency of non- and poorly coagulating milk in a herd of the three main Danish dairy breeds, Jersey, Danish red (RDM), and Danish Holstein-Friesian (SDM). The cows were milked by automatic milking. The rennet coagulation time, the coagulum firmness by storage modulus G', and the curd firming rate of fresh skimmed milk were determined. More than 20% of the milk samples exhibited poor coagulation properties by the criteria set. Of these, the 17% resulted in a weak coagulum after addition of rennet, while another 3% were due to NC milk that did not aggregate to form a curd within the 40 min of measurement. Milk from Jersey cows exhibited superior coagulation properties when compared to milk from SDM and RDM cows. The dependence of curd firming rate on protein concentration was investigated, and found to depend on milk protein concentration to the power of three, emphasizing the importance of protein interactions, and not just the protein concentration itself. The paper gives a basis for the subsequent selection of NC milk samples with theaim to look in more detail for the underlying reasons for the phenomenon of NC milk. © INRA and Springer Science+Business Media B.V. 2011.

van Otterloo J.,Monash University | Cas R.A.F.,Monash University | Sheard M.J.,Innovation
Bulletin of Volcanology | Year: 2013

The ∼5 ka Mt. Gambier Volcanic Complex in the Newer Volcanics Province, Australia is an extremely complex monogenetic, volcanic system that preserves at least 14 eruption points aligned along a fissure system. The complex stratigraphy can be subdivided into six main facies that record alternations between magmatic and phreatomagmatic eruption styles in a random manner. The facies are (1) coherent to vesicular fragmental alkali basalt (effusive/Hawaiian spatter and lava flows); (2) massive scoriaceous fine lapilli with coarse ash (Strombolian fallout); (3) bedded scoriaceous fine lapilli tuff (violent Strombolian fallout); (4) thin-medium bedded, undulating very fine lapilli in coarse ash (dry phreatomagmatic surge-modified fallout); (5) palagonite-altered, cross-bedded, medium lapilli to fine ash (wet phreatomagmatic base surges); and (6) massive, palagonite-altered, very poorly sorted tuff breccia and lapilli tuff (phreato-Vulcanian pyroclastic flows). Since most deposits are lithified, to quantify the grain size distributions (GSDs), image analysis was performed. The facies are distinct based on their GSDs and the fine ash to coarse+fine ash ratios. These provide insights into the fragmentation intensities and water-magma interaction efficiencies for each facies. The eruption chronology indicates a random spatial and temporal sequence of occurrence of eruption styles, except for a "magmatic horizon" of effusive activity occurring at both ends of the volcanic complex simultaneously. The eruption foci are located along NW-SE trending lineaments, indicating that the complex was fed by multiple dykes following the subsurface structures related to the Tartwaup Fault System. Possible factors causing vent migration along these dykes and changes in eruption styles include differences in magma ascent rates, viscosity, crystallinity, degassing and magma discharge rate, as well as hydrological parameters. © 2013 Springer-Verlag Berlin Heidelberg.

Gueimonde M.,Institute Productos Lacteos Of Asturias Csic | Florez A.B.,Institute Productos Lacteos Of Asturias Csic | Van Hoek A.H.A.M.,Wageningen University | Van Hoek A.H.A.M.,National Institute for Public Health and the Environment RIVM | And 4 more authors.
Applied and Environmental Microbiology | Year: 2010

All strains of Bifidobacterium animalis subsp. lactis described to date show medium level resistance to tetracycline. Screening of 26 strains from a variety of sources revealed the presence of tet (W) in all isolates. A transposase gene upstream of tet(W) was found in all strains, and both genes were cotranscribed in strain IPLAIC4. Mutants with increased tetracycline resistance as well as tetracycline-sensitive mutants of IPLAIC4 were isolated and genetically characterized. The native tet (W) gene was able to restore the resistance phenotype to a mutant with an alteration in tet(W) by functional complementation, indicating that tet(W) is necessary and sufficient for the tetracycline resistance seen in B. animalis subsp. lactis Copyright © 2010, American Society for Microbiology. All Rights Reserved.

Becker C.,University of Toronto | Faria L.,Innovation | Duretec K.,Vienna University of Technology
OCLC Systems and Services | Year: 2014

Purpose-Preservation environments such as repositories need scalable and context-aware preservation planning and monitoring capabilities to ensure continued accessibility of content over time. This article identifies a number of gaps in the systems and mechanisms currently available and presents a new, innovative architecture for scalable decision-making and control in such environments.Design/methodology/approach-The paper illustrates the state of the art in preservation planning and monitoring, highlights the key challenges faced by repositories to provide scalable decision-making and monitoring facilities, and presents the contributions of the SCAPE Planning and Watch suite to provide such capabilities.Findings-The presented architecture makes preservation planning and monitoring context-aware through a semantic representation of key organizational factors, and integrates this with a business intelligence system that collects and reasons upon preservation-relevant information. Research limitations/implications-The architecture has been implemented in the SCAPE Planning and Watch suite. Integration with repositories and external information sources provide powerful preservation capabilities that can be freely integrated with virtually any repository. Practical implications-The open nature of the software suite enables stewardship organizations to integrate the components with their own preservation environments and to contribute to the ongoing improvement of the systems.Originality/value-The paper reports on innovative research and development to provide preservation capabilities. The results enable proactive, continuous preservation management through a context-aware planning and monitoring cycle integrated with operational systems. © Emerald Group Publishing Limited.

Ristov S.,Ss. Cyril and Methodius University of Skopje | Gusev M.,Ss. Cyril and Methodius University of Skopje | Armenski G.,Ss. Cyril and Methodius University of Skopje | Velkoski G.,Innovation
IEEE Global Engineering Education Conference, EDUCON | Year: 2014

This paper describes the development of a scalable and elastic e-Assessment solution deployed on the OpenStack open source cloud. The solution activates virtual machine instances with a particular size for each assessment according to the number of students that should be assessed, in order to achieve sustainable performance. The system architecture and organization of this solution has been recently published [1]. We have improved this design by introducing two new agents: the Assessment Generation Agent and Assessment Commit Agent. These modules improve the solution by enabling strategies in the test generation process and preventing situations when the test results are not updated correctly. The developed solution has also enabled several new features, such as introducing complex questions with several learning objectives, better presentation of results, and developed a new method to eliminate guessing in multiple choice questions as an advantage in the defined negative marking schema. We also discuss several new challenges that occur within the cloud deployment, including reference to delivery of open courses to massive number of students. © 2014 IEEE.

Gusev M.,Ss. Cyril and Methodius University of Skopje | Ristov S.,Ss. Cyril and Methodius University of Skopje | Armenski G.,Ss. Cyril and Methodius University of Skopje | Gushev P.,Innovation | Velkoski G.,Innovation
IEEE Global Engineering Education Conference, EDUCON | Year: 2014

Besides the fact that e-Assessment systems can efficiently conduct all paper based tests to evaluate knowledge and skills, they can offer a lot of new features via sophisticated information and communication technologies, including adaptive testing, immediate evaluation, etc. Most of the realised e-Assessment systems use pictures in the realisation of e-Testing, but the pictures are mainly used as supported media enhancement of the multiple choice questions. We introduce a brand new idea to use interactive images, where the user can navigate and zoom the picture and provide answers by clicking on an appropriate graphical object, mark a region, annotate, set an answer/comment on a given position, etc. The application domain of this innovation is huge, including e-Assessment for those sciences, where image analysis is essential, such as analysis of medical images, gathering map selective user's opinion etc. We have developed three new question types based on interactive images that offer these innovations. The impact is not just in enhancement of offered technology, but also on preventing various cheating methods, such as memorising, guessing, etc. These innovations can improve the assessment results, by a more correct evaluation and knowledge assessment. © 2014 IEEE.

Zinno P.,University of Naples Federico II | Janzen T.,Innovation | Bennedsen M.,Innovation | Ercolini D.,University of Naples Federico II | Mauriello G.,University of Naples Federico II
International Journal of Food Microbiology | Year: 2010

Phage infection still represents the main cause of fermentation failure during the mozzarella cheese manufacturing, where Streptococcus thermophilus is widely employed as starter culture. Thereby, the success of commercial lactic starter cultures is closely related to the use of strains with low susceptibility to phage attack. The characterization of lytic S. thermophilus bacteriophages is an important step for the selection and use of starter cultures. The aim of this study was to characterize 26 bacteriophages isolated from mozzarella cheese plants in terms of their host range, DNA restriction profile, DNA packaging mechanism, and the variable region VR2 of the antireceptor gene. The DNA restriction analysis was carried out by using the restriction enzymes EcoRV, PstI, and HindIII. The bacteriophages were distinguished into two main groups of S. thermophilus phages (cos- and pac-type) using a multiplex PCR method based on the amplification of conserved regions in the genes coding for the major structural proteins. All the phages belonged to the cos-type group except one, phage 1042, which gave a PCR fragment distinctive of pac-type group. Furthermore, DNA sequencing of the variable region VR2 of the antireceptor gene allowed to classify the phages and examine the correlation between typing profile and host range. Finally, bacterial strains used in this study were investigated for the presence of temperate phages by induction with mitomycin C and only S. thermophilus CHCC2070 was shown to be lysogenic. © 2010 Elsevier B.V. All rights reserved.

Bennedsen M.,Innovation | Stuer-Lauridsen B.,Innovation | Danielsen M.,Innovation | Johansen E.,Innovation
Applied and Environmental Microbiology | Year: 2011

Second-generation genome sequencing and alignment of the resulting reads to in silico genomes containing antimicrobial resistance and virulence factor genes were used to screen for undesirable genes in 28 strains which could be used in human nutrition. No virulence factor genes were detected, while several isolates contained antimicrobial resistance genes. © 2011, American Society for Microbiology.

Laga N.,Innovation | Ditini N.,Innovation
2015 18th International Conference on Intelligence in Next Generation Networks, ICIN 2015 | Year: 2015

Business Process Management (BPM) and context awareness are two big research subjects. The former aims to control and assist the user in achieving his business goal, while the later aims to enhance the user experience by providing the right information, at the right moment, to the right person... etc. In this paper, we believe that live communications are relevant source of information to both business process management solutions and context aware applications. Based on this assumption, we propose a functionality recommender component which analyzes live communications to recommend functionalities to the user, taking into account his business process and its state. We applied the solution to the customer relationship management (CRM) field. More specifically, we applied it to assists customer care operator while in communication with a client. In this paper, we detail the architecture and the current implementation; we summarize our feedback; and we highlight the remaining research challenges. © 2015 IEEE.

Derkx P.M.F.,Innovation | Janzen T.,Innovation | Sorensen K.I.,Innovation | Christensen J.E.,Innovation | And 2 more authors.
Microbial Cell Factories | Year: 2014

The food industry is constantly striving to develop new products to fulfil the ever changing demands of consumers and the strict requirements of regulatory agencies. For foods based on microbial fermentation, this pushes the boundaries of microbial performance and requires the constant development of new starter cultures with novel properties. Since the use of ingredients in the food industry is tightly regulated and under close scrutiny by consumers, the use of recombinant DNA technology to improve microbial performance is currently not an option. As a result, the focus for improving strains for microbial fermentation is on classical strain improvement methods. Here we review the use of these techniques to improve the functionality of lactic acid bacteria starter cultures for application in industrial-scale food production. Methods will be described for improving the bacteriophage resistance of specific strains, improving their texture forming ability, increasing their tolerance to stress and modulating both the amount and identity of acids produced during fermentation. In addition, approaches to eliminating undesirable properties will be described. Techniques include random mutagenesis, directed evolution and dominant selection schemes. © 2014 Derkx et al.; licensee BioMed Central Ltd.

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