Innovatecs Biotechnology Research and Development

São Carlos, Brazil

Innovatecs Biotechnology Research and Development

São Carlos, Brazil

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Costa L.M.M.,Rua Santa Adelia | De Olyveira G.M.,Rua Santa Adelia | Basmaji P.,Innovatecs Biotechnology Research and Development | Filho L.X.,Natural Products Laboratory and Biotechnology
Journal of Bionanoscience | Year: 2011

Nanobiocellulose has established to be a remarkably versatile biomaterial and can be used in wide variety of applied scientific endeavours, especially for medical devices. In fact, biomedical devices recently have gained a significant amount of attention because of an increased interest in tissueengineered products for both wound care and the regeneration of damaged or diseased organs. Due to its unique nanostructure and properties, microbial cellulose is a natural candidate for numerous medical and tissue-engineered applications. The architecture of nanobiocellulose materials can be engineered over length scales ranging from nano to macro by controlling the biofabrication process. In this work, bacterial cellulose biocomposites were obtained by change fermentation medium with sugar cane, honey and dates paste (Dibs). SEM and AFM images showed differents surface morphology. FTIR analysis found some interactions between these additives. DSC and TGA showed higher thermal properties and change crystallinity of the developed bionanocomposite. Copyright © 2011 American Scientific Publishers.


Costa L.M.M.,Federal University of ABC | de Olyveira G.M.,Federal University of ABC | Basmaji P.,Innovatecs Biotechnology Research and Development | Valido D.P.,IPT UNIT | And 3 more authors.
Journal of Biomaterials and Tissue Engineering | Year: 2012

In the present work, we report the first bionanocomposite material formed by otoliths/bacterial cellulose (BC) networks (OCBC). This biomaterial is an osteoinductor or be, stimulates the bone regeneration, enabling bigger migration of the cells for formation of the bone fabric. Otolith is a typical biomaterial which is composed of calcium carbonate and organic matrix, present in the inner ear of fishes. Since they are rich in minerals considered essential to the bone mineralization process on a protein matrix (otolith). The objective in this study was to analyze the regeneration capacity of bone defects treated with nanotoliths/bc network preparation and evaluated the response of the dental pulps of dog teeth to capping in comparison with control group. Otoliths/bc network stimulates the formation of mineralized tissue barrier and inducing the reparative pulp response, and hence work as a promising pulp capping material. © 2012 American Scientific Publishers. All rights reserved.


Acasigua G.A.X.,Federal University of Rio Grande do Sul | de Olyveira G.M.,São Paulo State University | Costa L.M.M.,Federal University of ABC | Braghirolli D.I.,Federal University of Rio Grande do Sul | And 6 more authors.
Current Stem Cell Research and Therapy | Year: 2014

Bacterial cellulose (BC) has become established as a remarkably versatile biomaterial and can be used in a wide variety of applied scientific applications, especially for medical devices. In this work, the bacterial cellulose fermentation process is modified by the addition of hyaluronic acid and gelatin (1% w/w) to the culture medium before the bacteria is inoculated. Hyaluronic acid and gelatin influence in bacterial cellulose was analyzed using Transmission Infrared Spectroscopy (FTIR) and Scanning Electron Microscopy (SEM). Adhesion and viability studies with human dental pulp stem cells using natural bacterial cellulose/hyaluronic acid as scaffolds for regenerative medicine are presented for the first time in this work. MTT viability assays show higher cell adhesion in bacterial cellulose/gelatin and bacterial cellulose/ hyaluronic acid scaffolds over time with differences due to fiber agglomeration in bacterial cellulose/gelatin. Confocal microscopy images showed that the cell were adhered and well distributed within the fibers in both types of scaffolds. © 2014 Bentham Science Publishers.


Olyveira G.M.,Federal University of ABC | Acasigua G.A.X.,Federal University of Rio Grande do Sul | Costa L.M.M.,Federal University of ABC | Scher C.R.,Federal University of Rio Grande do Sul | And 3 more authors.
Journal of Biomedical Nanotechnology | Year: 2013

Adhesion and Viability study with human dental pulp stem cell using natural nanotolith/bacterial cellulose scaffolds for regenerative medicine are presented at first time in this work. Nanotolith are osteoinductors, i.e., they stimulate bone regeneration, enabling higher cells migration for bone tissue regeneration formation. This is mainly because nanotoliths are rich minerals present in the internal ear of bony fish. In addition, are part of a system which acts as a depth sensor and balance, acting as a sound vibrations detector and considered essential for the bone mineralization process, as in hydroxiapatites. Nanotoliths influence in bacterial cellulose was analyzed using transmission infrared spectroscopy (FTIR). Results shows that fermentation process and nanotoliths agglomeration decrease initial human dental pulp stem cell adhesion however tested bionanocomposite behavior has cell viability increase over time. Copyright © 2013 American Scientific Publishers All rights reserved.


Olyveira G.M.,Federal University of ABC | Costa L.M.M.,Federal University of ABC | Basmaji P.,Innovatecs Biotechnology Research and Development
Technical Proceedings of the 2011 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2011 | Year: 2011

Bacterial cellulose (BC) has established to be a remarkably versatile biomaterial and can be used in wide variety of applied scientific endeavors, especially for medical devices. In fact, biomedical devices recently have gained a significant amount of attention because of increased interesting tissue-engineered products for both wound care and the regeneration of damaged or diseased organs. The architecture of BC materials can be engineered over length scales ranging from nano to macro by controlling the biofabrication process. It has unique properties that make it an exciting candidate as a vascular graft material: strength, good integration into host tissue, and flexibility of production in various shapes and sizes. In this article, the structural features of microbial cellulose and its properties are discussed with AFM and SEM and novel bionanocomposites from BC/collagen and BC/chitosan are presented for artificial blood vessels by modifying culture medium of bacterial cellulose.


de Olyveira G.M.,Federal University of ABC | Costa L.M.M.,Federal University of ABC | Basmaji P.,Innovatecs Biotechnology Research and Development
Journal of Biomaterials and Tissue Engineering | Year: 2013

Bacterial cellulose (BC) has established to be a remarkably versatile biomaterial and can be used in wide variety of applied scientific endeavors, especially for medical devices. In fact, biomedical devices recently have gained a significant amount of attention because of increased interesting tissue-engineered products for both wound care and the regeneration of damaged or diseased organs. The architecture of BC materials can be engineered over length scales ranging from nano to macro by controlling the biofabrication process, besides, surface modifications bring a vital role in in vivo performance of biomaterials. This work highlights the potential of gamma irradiation treatment for the modification of the BC surface properties, enhancing its potential for biomedical applications. Samples did not show significant variation in thermal properties, however, higher density of porous were produced in irradiated samples than non irradiated which produced slowly diffusion than nonirradiated membrane. © 2013 American Scientific Publishers, All rights reserved.


Olyveira G.M.,São Paulo State University | Costa L.M.M.,Federal University of ABC | Basmaji P.,Innovatecs Biotechnology Research and Development
Journal of Biomaterials and Tissue Engineering | Year: 2013

Bacterial cellulose (BC) has established to be a remarkably versatile biomaterial and can be used in wide variety of applied scientific endeavors, especially for medical devices. In fact, biomedical devices recently have gained a significant amount of attention because of increased interesting tissue-engineered products for both wound care and the regeneration of damaged or diseased organs. The architecture of BC materials can be engineered over length scales ranging from nano to macro by controlling the biofabrication process, besides, surface modifications bring a vital role in in vivo performance of biomaterials. In this work, bacterial cellulose fermentation was modified with carbon nanotubes for sensor applications and diseases diagnostic. SEM images showed that polymer modified-carbon nanotube (PVOH-carbon nanotube) produced well dispersed system and without agglomeration. Influences of carbon nanotube in bacterial cellulose were analyzed by FTIR. TGA showed higher thermal properties of developed bionanocomposites. © 2013 American Scientific Publishers, All rights reserved.


Filho L.X.,Innovatecs Biotechnology Research and Development | Olyveira G.M.,Federal University of ABC | Basmaji P.,Innovatecs Biotechnology Research and Development | Costa L.M.M.,Federal University of ABC
Journal of Nanoscience and Nanotechnology | Year: 2013

Nanotholits is an osteoinductor or be, stimulates the bone regeneration, enabling bigger migration of the cells for formation of the bone tissue regeneration mainly because nanotholits are rich in minerals considered essential to the bone mineralization process on a protein matrix (otolin) as hydroxiapatite. In order to improve its biodegrability and bioresorption in new platforms for tissue engineering, it was electrospun PHB/nanotholits from aqueous solutions of this polymer at concentrations of nanotholits 1% (w/v) and compared morphological and thermal properties with PHB/nanotholits casting films. Electrospun PHB/nanotholits mats presents more symmetric nanopore structure than casting films mats observed by SEM images mainly because the orientation of pores along the longitudinal direction of the electrospun fibers. Nanotholits influences in PHB electrospun/casting was analyzed using transmission infrared spectroscopy (FTIR). TGA showed similar thermal properties but DSC showed distinct thermal properties and crystallinity process of the developed bionanocomposite mainly because of different processing. Copyright © 2013 American Scientific Publishers All rights reserved.


Costa L.M.M.,Federal University of ABC | de Olyveira G.M.,Federal University of ABC | Basmaji P.,Innovatecs Biotechnology Research and Development | Filho L.X.,Innovatecs Biotechnology Research and Development
Journal of Biomaterials and Tissue Engineering | Year: 2012

Bacterial cellulose (BC) has established to be a remarkably versatile biomaterial and can be used in wide variety of applied scientific endeavors, especially for medical devices. In fact, biomedical devices recently have gained a significant amount of attention because of increased interesting tissue-engineered products for both wound care and the regeneration of damaged or diseased organs. The architecture of BC materials can be engineered over length scales ranging from nano to macro by controlling the biofabrication process. It has unique properties that make it an exciting candidate as a medical material: strength, good integration into host tissue, and flexibility of production in various shapes and sizes. This review describes the fundamentals, purification and morphological investigation of bacterial cellulose. Furthermore, provides deep knowledge of current and applications of bacterial cellulose and their green nanocomposites to obtain a biomaterial with less rejection and better celular contact and blood contact cells interation in human and animal medicine. Besides, futures insights and researches in bacterial cellulose are discussed. © 2012 American Scientific Publishers. All rights reserved.


Basmaji P.,Innovatecs Biotechnology Research and Development | de Olyveira G.M.,São Paulo State University | dos Santos M.L.,São Paulo State University | Guastaldi A.C.,São Paulo State University
Journal of Biomaterials and Tissue Engineering | Year: 2014

Bacterial cellulose is a highly hydrated pellicle made up of a random assembly of ribbon shaped fibers less than 5 nm wide. The unique properties provided by the nanometric structure have led to a number of diagnostic biological probes, display devices due to their unique size-dependent medical applications. Bacterial cellulose matrix extracellular is a novel biotechnology and unique medicine indicated for ultimate chronic wound treatment management, drug delivery, tissue engineering, skin cancer and offers an actual and effective solution to a serious medical and social problem and to promote rapid healing in lesions caused by Diabetic burns, ulcers of the lower limbs or any other circumstance in which there's epidermal or dermal loss. In this work, it is reported novel antimicrobial peptides (AMPs) bacterial cellulose/polyhexanide biguanide (PHMB) which are produced by symbioses culture between polyhexanide biguanide and green tea culture medium resulting in the pure 3-D structure consisting of an ultra-fine network of novel biocellulose/PHMB nanofibres matrix (2-8 nm), highly hydrated (99% in weight), and with higher molecular weight, full biocompatibility. © 2014 American Scientific Publishers, All rights reserved.

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