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Carbonne, France

Fredonnet J.,French National Center for Scientific Research | Foncy J.,French National Center for Scientific Research | Foncy J.,National Polytechnic Institute of Toulouse | Foncy J.,French National Institute for Agricultural Research | And 15 more authors.
Microelectronic Engineering | Year: 2013

Microcontact printing (lCP) is used as a patterning technique to produce simple, rapid and cost-effective DNA microarrays. The accuracy of the final transferred pattern drastically depends on the inking step. The usual way to ink a PDMS stamp by droplet deposition of labeled biomolecules using a pipette, results in irregular transfer of the biomolecules on the chip surface and leads to poor and irreproducible fluorescent signals. These drawbacks are likely due to irregular 'coating' of the biomolecules on the PDMS stamp. In this work, a novel approach for inking PDMS with DNA molecules is presented. It is based on the continuous displacement of the meniscus formed by the inking solution over the surface of the stamp. When compared with the conventional technique, this dynamic PDMS inking method proved to be very reproducible for producing regular prints/spots on a functionalized glass slide, and this method could be easily extrapolated at an industrial scale. ©2013 Elsevier B.V. All rights reserved. Source

Egea A.M.C.,Roche Holding AG | Egea A.M.C.,French National Center for Scientific Research | Mazenq L.,Roche Holding AG | Trevisiol E.,French National Institute for Agricultural Research | And 6 more authors.
Microelectronic Engineering | Year: 2013

In this work, we use a label free biosensing technique based on the diffraction of light by molecular gratings. Molecular gratings are employed as diffractive probe arrays for protein interaction analysis. Indeed, the diffraction efficiency changes in response to analyte binding, due to a shift in line thickness and/or refractive index of the grating. In this perspective, we have developed a scanning instrument, capable of collecting at high speed the diffracted intensity emitted by molecular gratings. Nanogratings composed of anti-GST antibodies lines (500 nm width at a pitch of 1 lm) were immobilized through Microcontact printing on a PLL-g-dextran coated substrate. By monitoring the diffracted intensity of the anti-GST antibodies gratings, we have detected GST molecules at a concentration of 0.1 lM. This biodetection technique appears sensitive to low molecular weight molecules, such as GST (glutathione S-transferase, 26 kDa), which is an interesting result in comparison to other label free detection methods. © 2013 Elsevier B.V. All rights reserved. Source

Lagraulet A.,Innopsys
JALA - Journal of the Association for Laboratory Automation | Year: 2010

Microarrays used for measuring chromosomal aberrations in genomic DNA and for defining gene expression patterns have become almost routine. A microarray consists of an arrayed series of microscopic spots each containing either DNA or protein molecules known as feature reporters. Advances in microarray fabrication and in feature detection systems, such as high-resolution scanners and their associated software, lead to high-throughput screening of the genome or the transcriptome of a cell or a group of cells in only few days. Despite the potential of high-density microarrays, several problems about data interpretation are still to be solved. In addition, targeted microarrays are shown to be useful tools for rapid and accurate diagnosis of diseases. The aim of this review was to discuss the impact of microarrays on different application levels from the definition of disease biomarkers to pharmaceutical and clinical diagnostics. © 2010 Society for Laboratory Automation and Screening. Source

Innopsys | Date: 2002-11-26

Optical apparatus and instruments, namely, [ CD and DVD initializers, biochip optical readers, ] optical readers, optical scanners [ , optical lasers, optical disc players, CD and DVD readers and burners, optical printers, digital cameras for optical reading; computer software for use in the field of optical scanning, optical reading, data capture, data storage and data retrieval ]. [ Research and development services in the field of optical reading ].

Innopsys | Date: 2008-02-26

Image processing software for scientific, industrial and laboratory use in the field of biotechnology.

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