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Foncy J.,CNRS Laboratory for Analysis and Architecture of Systems | Colin C.,CNRS Laboratory for Analysis and Architecture of Systems | Degache A.,INNOPSYS | Esteve A.,CNRS Laboratory for Analysis and Architecture of Systems | And 5 more authors.
20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 | Year: 2016

Micro-contact printing is a key enabling technology for the development of molecular or cell based assays capture. However, the spreading of this technique at the industrial level is conditioned by the proof that large surfaces (> 1cm2) can be processed with different molecular inks and with a very high homogeneity. Here, we propose an original microfluidic approach that enables a reproducible inking and patterning of different molecular species (multiplexing) on large surfaces with a resolution at the sub-cellular level.

Fredonnet J.,French National Center for Scientific Research | Foncy J.,French National Center for Scientific Research | Foncy J.,National Polytechnic Institute of Toulouse | Foncy J.,French National Institute for Agricultural Research | And 15 more authors.
Microelectronic Engineering | Year: 2013

Microcontact printing (lCP) is used as a patterning technique to produce simple, rapid and cost-effective DNA microarrays. The accuracy of the final transferred pattern drastically depends on the inking step. The usual way to ink a PDMS stamp by droplet deposition of labeled biomolecules using a pipette, results in irregular transfer of the biomolecules on the chip surface and leads to poor and irreproducible fluorescent signals. These drawbacks are likely due to irregular 'coating' of the biomolecules on the PDMS stamp. In this work, a novel approach for inking PDMS with DNA molecules is presented. It is based on the continuous displacement of the meniscus formed by the inking solution over the surface of the stamp. When compared with the conventional technique, this dynamic PDMS inking method proved to be very reproducible for producing regular prints/spots on a functionalized glass slide, and this method could be easily extrapolated at an industrial scale. ©2013 Elsevier B.V. All rights reserved.

Egea A.M.C.,Hoffmann-La Roche | Egea A.M.C.,French National Center for Scientific Research | Mazenq L.,Hoffmann-La Roche | Trevisiol E.,French National Institute for Agricultural Research | And 6 more authors.
Microelectronic Engineering | Year: 2013

In this work, we use a label free biosensing technique based on the diffraction of light by molecular gratings. Molecular gratings are employed as diffractive probe arrays for protein interaction analysis. Indeed, the diffraction efficiency changes in response to analyte binding, due to a shift in line thickness and/or refractive index of the grating. In this perspective, we have developed a scanning instrument, capable of collecting at high speed the diffracted intensity emitted by molecular gratings. Nanogratings composed of anti-GST antibodies lines (500 nm width at a pitch of 1 lm) were immobilized through Microcontact printing on a PLL-g-dextran coated substrate. By monitoring the diffracted intensity of the anti-GST antibodies gratings, we have detected GST molecules at a concentration of 0.1 lM. This biodetection technique appears sensitive to low molecular weight molecules, such as GST (glutathione S-transferase, 26 kDa), which is an interesting result in comparison to other label free detection methods. © 2013 Elsevier B.V. All rights reserved.

Foncy J.,Hoffmann-La Roche | Crestel E.,Innopsys | Borges J.-P.,Hoffmann-La Roche | Esteve A.,Hoffmann-La Roche | And 8 more authors.
Microelectronic Engineering | Year: 2016

To provide a robust platform for fluid handling, most microfluidic devices usually involve irreversible bonding methods to achieve a leak free interface between the microchannels and the holding substrate. Such an approach induces a major drawback when biological interactions are performed on a microarray format as it is difficult to recover the biochip for further fluorescence scanner analysis. This work describes an automated microfluidic platform using a reversible magnetic clamp for multiplexed immunodiagnostics. The microfluidic device is composed of a magnetic PDMS layer (containing iron powder) coated by PDMS, which is reversibly clamped to an epoxysilane glass slide containing an array of various antigens. The microfluidic device was validated for in vitro diagnosis of food allergies on an allergen microarray after serum interaction. The statistical analysis of spot intensities (signal to noise ratios) on the microarray displayed excellent reproducibility. In addition to the reduction of volumes provided by miniaturization, this approach is versatile, is easy-to-produce and provides an effective platform for multiplexed immunodiagnosis based on conventional fluorescent detection schemes. © 2016 Elsevier B.V.

Cayron H.,Hoffmann-La Roche | Cayron H.,INSA Toulouse | Berteloite B.,Innopsys | Vieu C.,Hoffmann-La Roche | And 5 more authors.
Microelectronic Engineering | Year: 2015

Up to date, a large amount of research studies have been carried out to manipulate and arrange biomolecules at the single molecule scale using capillary forces. However, many of these techniques remain in the fundamental research field, their industrial transfer being restricted by poor repeatability and user-dependent processes. We present an automated process for the controlled and large scale deposition of single biomolecules, relying on the use of directed capillary assembly and nano-contact printing processes. The adjustment and control of physical parameters allow for single molecule deposition, and the use of an automate operating arm ensures high reproducibility and freedom in the assembly architectures to create. This methodology was used to assemble DNA molecules and actin filaments (or F-actin), evidencing two distinct assembly mechanisms. In the first one we use capillary forces to trap and elongate preformed 1D structures such as DNA molecules. In the second case, fluid flows created upon evaporation and local pinning of the meniscus favor the F-actin polymerization through continuous supply of monomers. In this way, we introduce a new concept of using capillary assembly as a construction tool to assemble 1D nano-structures. Additionally, by sequentially aligning and printing multiple single molecule assemblies, large-scale multi-layer architectures of single molecules were also obtained. The large-scale capabilities and reliability of our fabrication process render sophisticated single molecule biophysical measurements possible with systematic analysis over a large population for statistical relevance. © 2015 Elsevier B.V. All rights reserved.

Cau J.-C.,INNOPSYS | Ludovic L.,INNOPSYS | Marie N.,INNOPSYS | Adriana L.,INNOPSYS | Vincent P.,INNOPSYS
Microelectronic Engineering | Year: 2013

Microcontact printing (l-CP) is a well-known and easy-to-use technology which is well established worldwide. This technology opens new opportunities for various applications. Though there is a wide range of applications, there is no standardized or calibrated system to easily reproduce microcontact printing results or to transfer scientific research to industrial applications. One of the critical points affecting the quality of lCP is being able to control the force applied on the stamp during the printing step. Up until this point, existing technologies have been based on a mechanical force. However, the drawback to this system is that stamp geometry has to be adapted to the mechanical system. In this work, we propose a new concept of magnetic field assisted microcontact printing. We report theoretical and experimental studies of the homogeneity of the force applied and the resulting deposit. Theoretical models allow the prediction of a trend between the thickness of the magnetic stamp, the iron powder concentration and the pressure applied. The versatility of this concept is proven thanks to the development of an automated prototype, the INNOSTAMP40. © 2013 Elsevier B.V. All rights reserved.

Lagraulet A.,INNOPSYS
JALA - Journal of the Association for Laboratory Automation | Year: 2010

Microarrays used for measuring chromosomal aberrations in genomic DNA and for defining gene expression patterns have become almost routine. A microarray consists of an arrayed series of microscopic spots each containing either DNA or protein molecules known as feature reporters. Advances in microarray fabrication and in feature detection systems, such as high-resolution scanners and their associated software, lead to high-throughput screening of the genome or the transcriptome of a cell or a group of cells in only few days. Despite the potential of high-density microarrays, several problems about data interpretation are still to be solved. In addition, targeted microarrays are shown to be useful tools for rapid and accurate diagnosis of diseases. The aim of this review was to discuss the impact of microarrays on different application levels from the definition of disease biomarkers to pharmaceutical and clinical diagnostics. © 2010 Society for Laboratory Automation and Screening.

Innopsys | Date: 2014-08-22

Electronic systems, computer software and electronic printing apparatus.

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