New Orleans, LA, United States
New Orleans, LA, United States
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By utilizing a Mini-Primer strategy targeting the target site duplication (TSD) sequence of retrotransposons, insertion and null allele (INNUL) markers, which include short interspersed nuclear elements (SINEs), long interspersed nuclear elements (LINEs), and composite SVA retrotransposons (SINE/VNTR/Alu, where VNTR represents variable number of tandem repeats and Alu represents a type of primate specific SINE that has reached a copy number in excess of one million in the human genome), can be effectively used as markers for human identification and bio-ancestry studies regardless of the size of the inserted element. The size of the amplicons for INNULs and the difference between allelic states can be reduced substantially such that these markers have utility for analyzing high and low quality human DNA samples. Multiplexes including either 15 or 20 retrotransposable element (RE) markers plus Amelogenin for single tube amplification of DNA in four color detection were successfully designed. The multiplexes provided power of discrimination suitable for forensic and paternity analyses.


Grant
Agency: National Science Foundation | Branch: | Program: SBIR | Phase: Phase II | Award Amount: 500.00K | Year: 2012

This Small Business Innovative Research (SBIR) Phase II project will further develop the next-generation DNA test kits for forensic DNA testing. DNA profiling has developed significantly over the past 20 years and is routinely used to solve crime. Despite improvements in DNA testing technologies and equipment, a significant number of investigative biological samples do not yield informative results, due to excessive degradation of DNA. A significant number of crime scene and mass disaster samples are still unable to produce useful profiles due to current technology limitations. This research project utilizes a proprietary primer design approach in to analyze a significant number of degraded DNA samples. This project will also contribute to a basic understanding of human populations and their ancestral origins. The broader impact/commercial potential of this proposed project, if successful, will allow society to obtain answers to identification questions that could not previously be obtained, due to the limitations of current technology. Some of the benefits that can be expected from the development of this technology include: Solving more crimes using next generation DNA tests, including cases that were previously not testable, due to excessively degraded samples; assisting in the resolution of missing persons cases; provide closure to families of mass disaster victims such as the 9/11 terrorist attacks, Hurricane Katrina as well as identifying human remains of soldiers who lost their lives on foreign soil; provide ancestral origin information for individuals seeking information regarding their genealogy; provide a powerful intelligence tool that can be used for national security, military and in-combat efforts; and provide data for the educational and research studies of human anthropology. Overall, the resulting commercial impact of this transformational technology and the positive contribution it will have to society is immeasurable.


A method for processing forensic samples that include sperm cells from a perpetrator of sexual assault and epithelial cells that are primarily contributed by the victim is provided. The method includes providing a nanofiber filter that is formed of intermingled nanofibers having diameters of about 700 nm or less, selectively digesting the epithelial cells of a forensic sample and separating the sperm cells of the sample from the digested epithelial cells by filtration of the digest mixture through the nanofiber filter, the sperm cells becoming entrapped in the nanofiber filter. The captured sperm cells may then be digested to form a second digest mixture including digested sperm cell DNA. Using the first digest mixture filtrate and the second digest mixture, respectively, DNA samples may be isolated and DNA profiles may be obtained, the DNA profiles being useful for human identification. An apparatus and a kit for processing forensic samples obtained in sexual assault cases are also provided.


A retrotransposable element based multiplexed qPCR assay to robustly quantitate and distinguish cell free DNA integrity and concentration in blood plasma and serum is described. The multiplexed system for characterizing cancer in humans includes a sample of serum, plasma, urine, or other biological fluid, the sample comprising cell free DNA, the cell free DNA comprising long and short retrotransposable element targets and an added internal positive control, the long and short targets being independent of each other, a distinctly labeled TaqMan probe corresponding to each target, a forward primer and a reverse primer corresponding to each target, a DNA standard for generating standard curves, a qPCR system for amplifying the targets and a qPCR data analysis system. The assay provides an accurate, minimally-invasive, rapid, high-throughput, and cost-effective method with the potential to complement or replace existing methods for detection, diagnosis, prognosis, treatment monitoring and/or surveillance of cancer, thereby improving patient outcomes.


Trademark
InnoGenomics | Date: 2013-08-14

Pharmaceutical and Veterinary Preparations, Substances, Reagents, Media and Agents Adapted For Medical, Forensic, Human Identity, Research, Genetic Testing and Identification Uses, namely Degraded-DNA Assessment and Quantification Kits, DNA Assessment and Quantification Kits, Kits for Testing DNA And Degraded DNA in Biological Samples For Forensic, Human Identity, Research, Genetic Testing and Identification Uses, and Diagnostic Substances for Medical Uses, And Instructions For Using the Substances, Reagents, Preparations, Media, and Agents To Perform the Testing, in International Class 005.


Grant
Agency: Department of Health and Human Services | Branch: National Institutes of Health | Program: SBIR | Phase: Phase I | Award Amount: 300.00K | Year: 2015

Not Available


Grant
Agency: NSF | Branch: Standard Grant | Program: | Phase: | Award Amount: 150.00K | Year: 2011

This Small Business Innovation Research (SBIR) Phase I project proposes to develop a next generation forensic human DNA typing system. DNA profiling has gained broad popularity since its first use 25 years ago. Its application has expanded from criminal and parentage issues to mass disasters and battlefield forensics. In addition, the desire to rapidly analyze degraded and minimal samples has also increased exponentially. While DNA analysis of Short Tandem Repeats (STR) is considered routine, scientists continue to explore ways to identify smaller segments of DNA for identification purposes. Mitochondrial sequencing is presently the test of last resort but lacks the discrimination power of nuclear STR typing. As DNA profiling has increased, so has the need for quicker, more robust and less costly tests. This project proposes the use of a microfluidic platform with redesigned primers for the Alu family of Mobile Interspersed Genetic Elements. Alu primer sets producing amplicons in the 100-200 bp range will be typed for Alu insertion polymorphism. Due to the large number of Alus available in the human genome and their known ancestral state, these genetic elements can be a valuable tool for typing degraded DNA samples and in their ability to infer geographic origin.

The broader impact and commercial potential of this project is to provide a complete, robust, next generation DNA typing system for human identification. Successful development of a rapid Alu-based DNA typing system would provide the human identity testing market with additional and potentially transformational tools to identify biological samples containing degraded DNA as well as provide clues to the geographic ancestry of a specimen. Supplemental and next generation DNA typing systems will result in the production of more genetic information that can aid society in its search for the truth and justice for victims of violent crimes. Mass disasters, battlefield forensics, as well as routine DNA analysis for crime scene and parentage testing will benefit from a next generation rapid DNA identification system. For forensic applications, a microfluidic platform in conjunction with an Alu-based DNA typing system will address the commercial needs of this industry and at minimum could provide additional information to existing testing methods. Based upon the current human DNA identification testing market the commercial potential of a supplemental Alu-based DNA typing system is in excess of 100 million dollars, thus making a significant commercial impact and positive contribution to the field of human DNA profiling.


Grant
Agency: National Science Foundation | Branch: | Program: SBIR | Phase: Phase I | Award Amount: 150.00K | Year: 2011

This Small Business Innovation Research (SBIR) Phase I project proposes to develop a next generation forensic human DNA typing system. DNA profiling has gained broad popularity since its first use 25 years ago. Its application has expanded from criminal and parentage issues to mass disasters and battlefield forensics. In addition, the desire to rapidly analyze degraded and minimal samples has also increased exponentially. While DNA analysis of Short Tandem Repeats (STR) is considered routine, scientists continue to explore ways to identify smaller segments of DNA for identification purposes. Mitochondrial sequencing is presently the test of last resort but lacks the discrimination power of nuclear STR typing. As DNA profiling has increased, so has the need for quicker, more robust and less costly tests. This project proposes the use of a microfluidic platform with redesigned primers for the Alu family of Mobile Interspersed Genetic Elements. Alu primer sets producing amplicons in the 100-200 bp range will be typed for Alu insertion polymorphism. Due to the large number of Alus available in the human genome and their known ancestral state, these genetic elements can be a valuable tool for typing degraded DNA samples and in their ability to infer geographic origin. The broader impact and commercial potential of this project is to provide a complete, robust, next generation DNA typing system for human identification. Successful development of a rapid Alu-based DNA typing system would provide the human identity testing market with additional and potentially transformational tools to identify biological samples containing degraded DNA as well as provide clues to the geographic ancestry of a specimen. Supplemental and next generation DNA typing systems will result in the production of more genetic information that can aid society in its search for the truth and justice for victims of violent crimes. Mass disasters, battlefield forensics, as well as routine DNA analysis for crime scene and parentage testing will benefit from a next generation rapid DNA identification system. For forensic applications, a microfluidic platform in conjunction with an Alu-based DNA typing system will address the commercial needs of this industry and at minimum could provide additional information to existing testing methods. Based upon the current human DNA identification testing market the commercial potential of a supplemental Alu-based DNA typing system is in excess of 100 million dollars, thus making a significant commercial impact and positive contribution to the field of human DNA profiling.


Grant
Agency: NSF | Branch: Standard Grant | Program: | Phase: SMALL BUSINESS PHASE II | Award Amount: 876.00K | Year: 2012

This Small Business Innovative Research (SBIR) Phase II project will further develop the next-generation DNA test kits for forensic DNA testing. DNA profiling has developed significantly over the past 20 years and is routinely used to solve crime. Despite improvements in DNA testing technologies and equipment, a significant number of investigative biological samples do not yield informative results, due to excessive degradation of DNA. A significant number of crime scene and mass disaster samples are still unable to produce useful profiles due to current technology limitations. This research project utilizes a proprietary primer design approach in to analyze a significant number of degraded DNA samples. This project will also contribute to a basic understanding of human populations and their ancestral origins.

The broader impact/commercial potential of this proposed project, if successful, will allow society to obtain answers to identification questions that could not previously be obtained, due to the limitations of current technology. Some of the benefits that can be expected from the development of this technology include: Solving more crimes using next generation DNA tests, including cases that were previously not testable, due to excessively degraded samples; assisting in the resolution of missing persons cases; provide closure to families of mass disaster victims such as the 9/11 terrorist attacks, Hurricane Katrina as well as identifying human remains of soldiers who lost their lives on foreign soil; provide ancestral origin information for individuals seeking information regarding their genealogy; provide a powerful intelligence tool that can be used for national security, military and in-combat efforts; and provide data for the educational and research studies of human anthropology. Overall, the resulting commercial impact of this transformational technology and the positive contribution it will have to society is immeasurable.


Trademark
InnoGenomics | Date: 2013-08-15

Pharmaceutical and Veterinary Preparations, Substances, Reagents, Media and Agents Adapted For Medical, Forensic, Human Identity, Research, Genetic Testing and Identification Uses, namely Degraded-DNA Assessment and Quantification Kits, DNA Assessment and Quantification Kits, Kits for Testing DNA And Degraded DNA in Biological Samples For Forensic, Human Identify, Research, Genetic Testing and Identification Uses, and Diagnostic Substances Preparations, Reagents, Media and Agents for Medical Uses, And Instructions For Using the Substances, Reagents, Preparations, Media, and Agents To Perform the Testing, in International Class 005.

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