Chen L.,Inner Mongolia University |
Wang W.,Inner Mongolia Peoples Hospital |
Cao L.,Inner Mongolia University |
Li Z.,Inner Mongolia Medical College |
Wang X.,Inner Mongolia Medical College
Molecules and cells | Year: 2016
Long non-coding RNAs (lncRNAs) are involved in multiple cellular events, as well as in tumorigenesis. Colon cancer-associated transcript-1 (CCAT1) gene encodes an lncRNA whose over-activation was observed in an expanding list of primary human solid tumors and tumor cell lines, however its biological roles in acute myeloid leukaemia (AML) has not been reported yet at present. In this study, the aberrant upregulation of CCAT1 was detected in French-American-British M4 and M5 subtypes of adult AML patients. By gain- and loss-of-function analysis, we determined that CCAT1 repressed monocytic differentiation and promoted cell growth of HL-60 by sequestering tumor suppressive miR-155. Accordingly, a significant decrease in miR-155 level was detected in AML patients. Re-introduction of miR-155 into HL-60 cells restored monocytic maturation and repressed cell proliferation. Furthermore, CCAT1 could up-regulated c-Myc via its competing endogenous RNA (ceRNA) activity on miR-155. In conclusion, these results revealed new mechanism of lncRNA CCAT1 in AML development, and suggested that the manipulation of CCAT1 expression could serve as a potential strategy in AML therapy.
Shen J.,Inner Mongolia Peoples Hospital
Chinese Journal of Cerebrovascular Diseases | Year: 2016
Objective: To investigate the roles of platelet-derived growth factor β-receptor (PDGFR-β) signal in blood-brain barrier repair and functional recovery after cerebral ischemia. Methods: A total of 204 mice with post-birth condition specific system PDGFR-β gene knockout were used to make an ischemic model of middle cerebral artery occlusion with the photochemical method, the PDGFR-β knockout mice (Esr-KO ischemic group) and non-gene knockout mice (Floxed ischemic group) (n =93 in each group). The other 18 sham-operation models (without laser irradiation) were divided into an Esr-KO sham-operation group and a Floxed sham-operation group (n =9 in each group). At day 6 and 14 after ischemia, the volume of cerebral infarction of both groups were calculated. The pericytes related α-smooth muscle actin (α-SMA) , basement membrane protein laminin (laminin) expression level, perivascular cell coverage level ( α-SMA/laminin) ,and peripheral cell proliferation level (Ki67/α-SMA) in both group were compared and the permeability differences of blood-brain barrier after cerebral ischemia were analyzed. Results: (1) At day 6 and 14 after ischemia, the cerebral infarction volume of the Esr-KO ischemic group was significantly larger than that of the Floxed ischemic group. The difference between the two groups was statistically significant (all P<0.01). (2) At day 6 after ischemia, the expression of PDGFR-β and α-SMA in the ischemic border region and center region in the Esr-KO ischemic group was decreased significantly compared with the Floxed ischemic group (all P < 0.01) , and there was no significant difference in the expression of laminin (all P > 0. 05 ). The double immunofluorescence staining showed that the α;-SMA/laminin and Ki67/ α-SMA in the ischemic border region of the Esr-KO ischemic group were significantly lower than those of the Floxed ischemia group (15 ±5% vs. 43 ±3% ; t =4. 221 ,P <0. 01; 14. 2 ± 1. 7% vs. 43. 7 ±3. 9% ; t =3. 964,P <0. 01]. (3) The analysis of the green fluorescence tracing technique showed that the vascular i permeability of ischemic border region was increased significantly in the Esr-KO ischemia group compared with the Floxed ischemic group (at day 3:40. 1 ±1.2% vs. 25.5 ± 1. 7% ;t =3. 882,P <0. 01 ;at day 6: 90. 1 ±8.7% vs. 36. 5 ± 4. 5% ; t = 4. 957,P < 0. 01). Conclusion: The PDGFR-β signaling system may be partially involved in the processes of blood-brain barrier repair and functional recovery after cerebral ischemia.
Wang Z.,Inner Mongolia Peoples Hospital
Journal of Craniofacial Surgery | Year: 2017
ABSTRACT: The present study aims to explore the effectiveness of decompressive craniectomy with bifrontal coronal incision in the management of severe contusion and laceration of bilateral fronto-temporal lobes, as well as the outcomes of early cranioplasty. The authors performed the bifrontal decompressive craniectomy on 56 patients with contusion and laceration of bilateral frontal and temporal lobes, and their follow-up treatment outcomes were tracked within 6 months using Glasgow Outcome Scale. The results showed that 33 patients (out of 56, 58.9%) have recovered, 12 patients (out of 56, 21.4%) have moderate defects, 5 patients (out of 56, 8.9%) have severe defects, 3 patients (out of 56, 5.3%) stayed in persistent vegetative status, and the remaining 3 patients (out of 56, 5.3%) have been dead. There was no persistent temporal hollowing. No patients required revision surgery with modified titanium mesh in this study. Particularly, 28 patients have successfully accepted the early cranioplasty with bone flap or computer-assisted design titanium mesh, and showed good recovery. These results together indicated that the decompressive craniectomy with bifrontal coronal incision in the management of severe contusion and laceration of bilateral fronto-temporal lobes can significantly relieve the comorbidity of intracranial hypertension, and improve the prognosis obviously, thus finally increasing the probability of successful rescue and decreasing the probability of mortality and disability. © 2017 by Mutaz B. Habal, MD.
Lin J.,China Japan Friendship Hospital |
Song X.,Inner Mongolia Peoples Hospital |
Liu C.,Peking University
International Journal of Gynecological Pathology | Year: 2014
Benign metastasizing leiomyoma and intravascular leiomyomatosis are both rare smooth-muscle proliferations that of special interest due to their quasi-malignant behaviors. To elucidate the pathogenesis of these lesions, we checked the surgical samples from a 43-year-old female presenting multiple pulmonary and tricuspid valve lesions after a history of hysterectomy. Histopathological studies confirmed pelvic intravascular leiomyomatosis in the hysterectomy sections and the pulmonary nodules were proved to be benign metastasizing leiomyoma with strong positivity of estrogen as well as progesterone receptors. Clonality and copy number variance analysis, which were performed on pulmonary and uterine tumors, showed an identical X-chromosome inactivation pattern and a balanced karyotype respectively. Sequential occurrence of benign metastasizing leiomyoma and intravascular leiomyomatosis in same patient implied they were histogenetically related and our molecular genetic proofs further suggested that benign metastasizing leiomyoma is a unicentric, benign metastasizing process arising from initial intravascular leiomyomatosis. © 2014 International Society of Gynecological Pathologists.
Wang Y.,Inner Mongolia Agricultural University |
Li Y.,Inner Mongolia Agricultural University |
Xie J.,Inner Mongolia Peoples Hospital |
Zhang Y.,Inner Mongolia Agricultural University |
And 3 more authors.
International Immunopharmacology | Year: 2013
Lactobacillus casei Zhang (LcZ) has been recently isolated from the traditional Mongolian beverage koumiss and has a set of favorable probiotic properties, including aciduricity, bile resistance and ability to colonize the gastrointestinal tract. We have previously reported the anti-oxidative properties of LcZ in the hyperlipidemic rats. In this study, the hepatoprotective effects of LcZ against lipopolysaccharide (LPS) and d-galactosamine (D-GalN)-induced liver injury were investigated. We found that pretreatment with LcZ significantly improved survival of rats challenged with LPS/D-GalN. In addition, pretreatment with LcZ significantly decreased alanine transaminase (ALT) and aspartate aminotransferase (AST) levels in LPS/D-GalN-challenged rats, which were accompanied by diminished liver injuries, reduced malondialdehyde (MDA) content and increased superoxide dismutase (SOD) activity in liver homogenates. Pretreatment with LcZ also markedly reduced LPS/D-GalN-induced production of hepatic nitric oxide (NO), activation of inducible nitric oxide synthase (iNOS) and expression of tumor necrosis factor-α (TNF-α). Furthermore, hepatic toll-like receptor 4 (TLR4) mRNA and protein levels, the phosphorylation of I-κB and translocation of nuclear factor κB (NF-κB) were significantly down-regulated by pretreatment with LcZ. These results suggest that pretreatment with LcZ protects against LPS/D-GalN-induced liver injury in rats via its anti-oxidative and anti-inflammatory capacities. The hepatoprotective effects of LcZ are associated with an inhibition of TLR4 expression and TLR4 signaling. © 2012 Elsevier B.V. All rights reserved.
Xu Q.,Inner Mongolia Peoples Hospital
World Automation Congress Proceedings | Year: 2014
Along with widely application of the network and information techniques, the level of informatization in libraries has been developed, however, the security problems also become severe. Based on the analysis of current security condition of library network and the key factors by which security disasters can be induced, a lightweight authentication protocol is proposed, ensuring only the legal users can access into the digital library. The analysis indicates that the protocol is both efficient and secure. © 2014 TSI Press.
Bai R.,Inner Mongolia Peoples Hospital
Cellular and molecular biology (Noisy-le-Grand, France) | Year: 2012
Ghrelin has recently garnered increasing attention in biomolecular studies. Ghrelin's growth hormone secretagogue receptor type 1a (GHS--R) is a pleiotropic modulator of diverse biological functions, including energy homeostasis and reproduction. This study sought to understand the ways in which ghrelin impacts ERK1/2 and p90rsk during the ovis aries oocyte maturation process. We applied different concentrations of ghrelin and of ghrelin receptor inhibitor (D--Lys3--GHRP--6) to ovis aries oocytes and observed the effects on the ERK1/2 and p90rsk pathway. The ERK1/2 and p90rsk pathway plays an essential role in the in vitro maturation of ovis aries oocytes. This study discovered that ERK1/2 and p90 rsk pathway, during the ovis aries oocyte maturation, was associated with maturation of ovis aries oocyte in vitro.
Chi N.,Inner Mongolia Peoples Hospital |
Tan Z.,Inner Mongolia Peoples Hospital |
Ma K.,Inner Mongolia Peoples Hospital |
Bao L.,Inner Mongolia University |
Yun Z.,Inner Mongolia Peoples Hospital
International Journal of Clinical and Experimental Medicine | Year: 2014
Aim: Myeloid-derived suppressor cells (MDSCs) are a population of cells which negatively regulate immune response during tumor progression. In this study, we assessed the accumulation of MDSCs (CD33+CD11b+HLA-DR-CD14-) in patients with prostate cancer and its clinical relevance. Methods: We tested the frequency of MDSCs in the peripheral blood of patients with prostate cancer or benign prostate hyperplasia and healthy donors. Serumal interleukin-8, -6 and -10 were analyzed. Effects of MDSCs on the T cell response were determined. Results: MDSCs increased in cancer patients, and there was an association between MDSCs and cancer stages or overall survival. Elevated serumal interleukin-8 and -6 in cancer patients correlated with MDSCs. Moreover, accumulation of MDSCs was associated with defective T cell function. Conclusion: Our study showed an increased population of MDSCs in patients with prostate cancer. Interleukin-8 and -6 in serum may play a new important role companied with MDSCs in prostate cancer. © 2014, E-Century Publishing Corporation. All rights reserved.
Zhang Y.,Inner Mongolia Peoples Hospital |
Zhang Y.,Tianjin Medical University |
Ju W.,Inner Mongolia Peoples Hospital |
Ju W.,Tianjin Medical University |
And 6 more authors.
Obesity Surgery | Year: 2015
Laparoscopic Roux-en-Y gastric bypass (LRYGB) and laparoscopic sleeve gastrectomy (LSG) are two most common weight loss procedures; our meta-analysis aims to compare these two in the treatment of morbid obesity and its related comorbidities. An electronic literature research of published studies concerning LRYGB and LSG was performed from inception to October 2013. Percentage of excess weight loss (%EWL), resolution or improvement rate of comorbidities, and adverse events were all pooled and compared by the software Review Manager 5.1. As a result, a total of 21 studies involving 18,766 morbidly obese patients were eventually selected according to the inclusion criteria. No significant difference was found in %EWL during 0.5- to 1.5-year follow-up (P > 0.05), but after that, LRYGB achieved higher %EWL than LSG (P < 0.05). Except for type 2 diabetes mellitus (T2DM) (P < 0.001), the difference between these two procedures in the resolution or improvement rate of other comorbidities did not reach a statistical significance (P > 0.05). There were more adverse events in LRYGB compared with LSG (P < 0.01). In conclusion, LRYGB is superior to LSG in efficacy but inferior to LSG in safety. © 2014, Springer Science+Business Media New York.
Li R.,Inner Mongolia Peoples Hospital |
Zhang L.,Inner Mongolia Peoples Hospital |
Jia L.,Inner Mongolia University |
Duan Y.,Inner Mongolia Peoples Hospital |
And 3 more authors.
PLoS ONE | Year: 2014
Long non-coding RNAs (lncRNAs) have been shown to be implicated in the complex network of cancer including malignant melanoma and play important roles in tumorigenesis and progression. However, their functions and downstream mechanisms are largely unknown. This study aimed to investigate whether BRAF-activated non-coding RNA (BANCR), a novel and potential regulator of melanoma cell, participates in the proliferation of malignant melanoma and elucidate the underlying mechanism in this process. We found that BANCR was abnormally overexpressed in human malignant melanoma cell lines and tissues, and increased with tumor stages by quantitative PCR. BANCR knockdown induced by shRNA transfection significantly inhibited proliferation of tumor cells and inactivated MAPK pathway, especially by silencing the ERK1/2 and JNK component. Moreover, combination treatment of BANCR knockdown and suppression ERK1/2 or JNK (induced by specific inhibitors U0126 or SP600125 respectively) produced synergistic inhibitory effects in vitro. And the inhibitory effects induced by ERK1/2 or JNK could be rescued by BANCR overexpression. By tumorigenicity assay in BALB/c nude mice, we further found that BANCR knockdown inhibited tumor growth in vivo. In addition, patients with high expression of BANCR had a lower survival rate. Taken together, we confirmed the abnormal upregulation of a novel lncRNA, BANCR, in human malignant melanoma. BANCR was involved in melanoma cell proliferation both in vitro and in vivo. The linkage between BANCR and MAPK pathway may provide a novel interpretation for the mechanism of proliferation regulation in malignant melanoma. © 2014 Li et al.