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Niu Y.,Inner Mongolia University | Niu Y.,Inner Mongolia Key Laboratory of Herbage and Endemic Crop Biotechnology | Hu T.,Inner Mongolia University | Zhou Y.,Inner Mongolia University | And 2 more authors.
Plant Physiology and Biochemistry | Year: 2010

DREB transcription factors play an important role in tolerance to abiotic stress in high plants. In this work, two new DRE-binding protein genes MfDREB1 and MfDREB1s cDNA that encoded an AP2/EREBP type transcription factor were isolated by RT-PCR from Medicago falcate seedlings. Sequence analysis showed MfDREB1 and MfDREB1s were almost identical except that there was a 202. bp fragment at the 3′ end of the MfDREB1s cDNA that is absent in MfDREB1 cDNA. The MfDREB1 has a open reading frame of 651. bp, which encodes 216 amino acid residues. The putative protein is deduced a predicted molecular mass of 24.6. kDa and a pI of 5.95. The MfDREB1s has a open reading frame of 555. bp, the putative protein is 184 amino acid long with a predicted molecular weight of 20.8. kDa, pI 9.11. The Protein Blast data revealed that the two proteins can be classified as a typical member of the AP2/EREBP family of DNA-binding proteins. The comparison of the MfDREB1 cDNA and MfDREB1s cDNA with their corresponding genes in genomic DNA showed that the size and nucleotide sequence of the cDNA was identical to that of the genomic DNA. This suggested that the genomic MfDREB1 gene and MfDREB1s gene had no introns. Southern blot analysis indicated that MfDREB1 and MfDREB1s are multi-copy genes in Medicago falcate genome. Northern blot analysis indicated that the MfDREB1 and MfDREB1s genes were induced by low temperature stress. © 2010 Elsevier Masson SAS.

Gao F.,Inner Mongolia University | Gao F.,Inner Mongolia Key Laboratory of Herbage and Endemic Crop Biotechnology | Hao J.,Inner Mongolia University | Hao J.,Inner Mongolia Key Laboratory of Herbage and Endemic Crop Biotechnology | And 5 more authors.
Russian Journal of Plant Physiology | Year: 2013

Melon is an ideal alternative model fruit to examine ethylene perception and sensitivity. Ethylene insensitive 2 (EIN2), an integral membrane protein in the endoplasmic reticulum, is an important regulator of ethylene and other phytohormone signaling. We isolated for the first time a cDNA clone that encoded EIN2 homolog on the basis of melon (Cucumis melo L. cv. Hetao) fruit total RNA by in silico cloning and reverse-transcription PCR (RT-PCR). The cDNA contained an open reading frame of 3876 bp corresponding to a polypeptide of 1291 amino acids with a predicted mol wt of 141 kD. The expression patterns of different developmental stages of fruit, vegetative organs, and reproductive tissues and upon the treatment with IAA and ABA were analyzed. CmEIN2 mediates ethylene signals in many processes and is a component of signal transduction by ethylene, auxin, and abscisic acid. © 2013 Pleiades Publishing, Ltd.

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