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Wang B.P.,Inner Mongolia Agricultural University | Wang B.P.,Inner Mongolia Key Laboratory of Bio Manufacture | Zhang D.,Inner Mongolia Agricultural University | Zhang D.,Inner Mongolia Key Laboratory of Bio Manufacture | And 27 more authors.
Journal of Animal and Veterinary Advances | Year: 2013

The current study investigated the ovarian response to gonadotropin for establishing a suitable protocol of superovulation in Bactrian camel. Fifteen female camels were randomly divided into 4 groups to compare 4 different superovulation protocols during the natural breeding season. Each camel in 4 groups was injected with FSH at 80, 80, 60, 60, 60, 60, 40 and 40 mg, respectively total dosage of 480 mg, for consecutive 4 days at 12 h intervals. The camels in group 2-4 were naturally mated and subsequently injected 300 IU LH 48 h after the last injection of FSH, the camels in group 1 received the same procedure exception of LH injection. Ovarian follicles and corpora luteas were observed through synchronistic laparotomy 7-9 days after natural mating. The results indicated that there is no significant difference in average ovulation rate (p > 0.05) among group 1-3 nor among group 2-4. However, there is considerable difference between group 1 and 4 in average ovulation rate (p < 0.05), among which the value is highest (81.38±6.44%) for group 4 but lowest (16.89±7.98%) for group 1. Furthermore, the average number of follicles has yet no obvious difference (p > 0.05) among group 1-3 nor among group 2-4 but significant difference (p < 0.05) between group 1 and 4. Comparatively the animals in group 1 yielded highest average number of follicles (9.80±1.50). Conclusively, the protocol 4 had a best superovulatory effect with an average corpora lutea 9.33±1.45 and therefore it can be used for superovulation in camels. © Medwell Journals, 2013.

Wang F.,Inner Mongolia Agricultural University | Wang F.,Inner Mongolia Key Laboratory of Bio Manufacture | Wang F.,Nankai University | Pan J.,Inner Mongolia Agricultural University | And 15 more authors.
Progress in Biochemistry and Biophysics | Year: 2016

Current research has determined that many cloned animals have heterogeneous DNA methylation profiles. However, few studies have compared the methylation profiles of both naturally produced lambs and cloned lambs created using somatic cell nuclear transfer. The paucity of research in this area is because of insufficient resources to study limited cloned offspring, the ovine genome, and ovine genomic imprinting. In this study, to show the degree of reprogramming in cloned lambs, we cloned the putative differentially methylated regions (DMRs) of Peg3 from sheep and analyzed the DNA methylation patterns in CpG islands and DMRs of the putative imprinted genes Peg3, Cdknlc and Gtl2 in cloned lambs. We have provided evidence that Peg3 was highly methylated. The degree of methylation was 95.45% in the kidney and 88.18% in the lung of a natural sheep and 98.18% in the kidney and 87.27% in the lung for one cloned sheep. The bisulphite sequencing results for Cdknlc show complete non-methylation (0%, 0.53%, 0.53%, 0.53%) in all samples. In addition, Gtl2 was hypomethylated in all lambs, from a linear correlation analysis, there were some differences in the quantitative values from both groups (correlation r2 = 0.77). These data show that the DNA methylation status of the three imprinted genes was similar in cloned and natural lambs.

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