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Guan J.,Inner Mongolia International Mongolian Hospital | Yuan S.,Inner Mongolia Medical College | Wu H.,Inner Mongolia International Mongolian Hospital | Na R.,Inner Mongolia International Mongolian Hospital | And 3 more authors.
PLoS ONE | Year: 2017

Background and objective: To systematically evaluate the clinical effects and safety of traditional Chinese medical bath therapy (TCMBT) combined with ultraviolet irradiation in the treatment of psoriasis. Methods: Electronic database retrieval was utilized. The foreign retrieval databases consulted included those of the Cochrane Library, PubMed and EMBASE; the domestic retrieval databases included the Chinese Biomedical Literature Database (Sino-Med), the China National Knowledge Infrastructure (CNKI), VIP and the WangFang Database. Clinical randomized controlled trials were conducted to evaluate the effects of TCMBT combined with ultraviolet irradiation in the treatment of psoriasis; the language of the retrieved articles was Chinese or English. Each database was searched from its inception to August 1, 2015. Two researchers independently collected the data and analyzed the methodology of the documented literature. The researchers conducted a meta-analysis with RevMan 5.2.3 software. Results: According to the available literature, 25 RCTs (randomized controlled trials) of low research quality were conducted. According to the meta-analysis, the total effective rate of TCMBT combined with ultraviolet irradiation was relatively higher than that of ultraviolet irradiation alone. The recurrence rate, incidence of adverse reactions and Psoriasis Area and Severity Index (PASI) for the combined therapy was lower than that of ultraviolet irradiation (P<0.05). Conclusion: For the treatment of psoriasis, the clinical effects and safety of TCMBT combined with ultraviolet irradiation are generally better than those of ultraviolet irradiation alone. However, the original literature was written in Chinese, and the quality of the studies was not high. Thus, it is difficult to confirm the clinical effects and safety of TCMBT combined with ultraviolet irradiation. It is necessary to conduct a scientific, normalized and high-quality RCT with multiple large samples and centers. © 2017 Guan et al.This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


PubMed | Inner Mongolia International Mongolian Hospital, Copenhagen University, China James D Watson Institute Of Genome Science, BGI Shenzhen and 6 more.
Type: Journal Article | Journal: Genome biology and evolution | Year: 2014

Mongolians have played a significant role in modern human evolution, especially after the rise of Genghis Khan (1162[?]-1227). Although the social cultural impacts of Genghis Khan and the Mongolian population have been well documented, explorations of their genome structure and genetic imprints on other human populations have been lacking. We here present the genome of a Mongolian male individual. The genome was de novo assembled using a total of 130.8-fold genomic data produced from massively parallel whole-genome sequencing. We identified high-confidence variation sets, including 3.7 million single nucleotide polymorphisms (SNPs) and 756,234 short insertions and deletions. Functional SNP analysis predicted that the individual has a pathogenic risk for carnitine deficiency. We located the patrilineal inheritance of the Mongolian genome to the lineage D3a through Y haplogroup analysis and inferred that the individual has a common patrilineal ancestor with Tibeto-Burman populations and is likely to be the progeny of the earliest settlers in East Asia. We finally investigated the genetic imprints of Mongolians on other human populations using different approaches. We found varying degrees of gene flows between Mongolians and populations living in Europe, South/Central Asia, and the Indian subcontinent. The analyses demonstrate that the genetic impacts of Mongolians likely resulted from the expansion of the Mongolian Empire in the 13th century. The genome will be of great help in further explorations of modern human evolution and genetic causes of diseases/traits specific to Mongolians.


Dalai N.,Jilin University | Cui H.,Inner Mongolia University | Yan M.,Inner Mongolia University | Rile G.,Inner Mongolia International Mongolian Hospital | And 2 more authors.
Genetics and Molecular Research | Year: 2014

Lifestyle, habits, diet, and genetics are all important factors associated with the prevalence of hypertension. Many association studies have been performed in the Chinese Han population, whereas data explaining the high prevalence of hypertension in the Mongolian population remain scarce. In the present study, we aimed to determine the factors associated with the development of essential hypertension in Mongolians. A total of 194 hypertensive cases and 201 controls from Dongwu County were enrolled in the study. Demographics, anthropometric and blood biochemical parameters, food intake, lifestyle, habits, education, occupation, and family history were recorded for each subject. Genotype and allele frequencies of six single nucleotide polymorphisms (SNPs) of the kallikrein 1 (KLK1) gene were also examined. Mean body mass index, waistline, hipline, blood sugar, blood urea nitrogen, creatinine, uric acid, total cholesterol, triglyceride, and low-density lipoprotein levels were all significantly higher in the hypertensive group (P < 0.01). Hypertensives consumed less milk, vegetables, and fruits, and had higher cigarette, alcohol, and salt intake (P < 0.05). There were also less regular physical exercisers and manual workers among the hypertensive group (P < 0.05). The mean inheritance rank of the hypertensive group was higher than that of controls (P < 0.05). There were no differences in the distribution of genotype and allele frequencies of the six SNPs between the hypertensive and control groups (P > 0.05). These results suggest that dietary history and habits have the most important influence on the development of essential hypertension in the Mongolian population. © FUNPEC-RP.


Guan J.,Inner Mongolia International Mongolian Hospital | Du Q.,Guangdong Medical University | Li Q.,Inner Mongolia University | Liu X.,Liaoning Province Chinese Medicine Research Institute | Yuan S.,Inner Mongolia Medical College
International Journal of Clinical and Experimental Medicine | Year: 2016

Background: The efficacy of aspirin combined with low molecular weight heparin in the treatment of habitual abortion is controversial. The efficacy of aspirin combined with low molecular weight heparin versus aspirin alone for habitual abortion is still unclear. Aim: The purpose is to compare the efficacy of low molecular weight heparin therapy versus aspirin monotherapy for habitual abortion. Methods: The PubMed, EMBASE, Cochrane Library, Web of Science, Chinese National Knowledge Infrastructure (CNKI) and Chinese Biology Medicine (CBM), VIP database and Wanfang database were searched to identify randomized controlled trials (RCTs) about aspirin combined with low molecular weight heparin in the treatment of habitual abortion without date or language restrictions (last search up to April, 2016) and reference lists of relevant reviews were checked to identify additional studies. We calculated relative risk (RR) with 95% confidence interval (CI) using Stata 14.0 software. Results: The results of meta-analysis suggested that combination therapy improved the live birth rate [RR, 1.18, 95% CI, (1.06, 1.31), I2=0%, P=0.002] and significantly reduced abortion rate [RR, 0.55, 95% CI, (0.44, 0.69), I2=2.1%, P=0.000] compared with aspirin monotherapy. Conclusion: Combination therapy shows benefic effects in the treatment of women with habitual abortion and is better than aspirin alone therapy. Further study is required to confirm these findings. © 2016, E-Century Publishing Corporation. All rights reserved.


Liu Y.,Inner Mongolia University | Ao N.,Inner Mongolia International Mongolian Hospital | Zhao H.,Inner Mongolia University
Experimental and Therapeutic Medicine | Year: 2016

Congenital absence of gallbladder and atrial septal defect (ASD) are clinically rare congenital organ malformations, and the simultaneous occurrence of the two is even more rare. The present study reported a case of gallbladder agenesis combined with congenital ASD. A 38-year-old male patient presented with a 3-year history of recurring upper right abdominal pain. The pain had no evident cause and was accompanied by dyspepsia and gasteremphraxis with indigestion. Several color Doppler ultrasonography scans revealed cholecystitis and gallbladder stones. A physical examination revealed cardiac murmur. A color Doppler ultrasonography of the heart was indicative of congenital heart disease. A corrective surgery for ASD was performed. Subsequently, a mini-incision cholecystectomy was performed as explorative surgery. A magnetic resonance cholangiopancreatography scan of the abdomen was performed in order to confirm the diagnosis of gallbladder disorder and cystic duct hypoplasia. A final definite diagnosis of gallbladder agenesis was confirmed. In conclusion, atrophy and gallbladder full of stones are frequently misdiagnosed, and establishing a definitive preoperative diagnosis is difficult. Awareness of this congenital malformation may assist physicians in distinguishing cases with unclear manifestation and avoiding unnecessary surgical interventions. © 2016, Spandidos Publications. All rights reserved.


PubMed | Inner Mongolia International Mongolian Hospital and Inner Mongolia University
Type: Journal Article | Journal: Experimental and therapeutic medicine | Year: 2016

Congenital absence of gallbladder and atrial septal defect (ASD) are clinically rare congenital organ malformations, and the simultaneous occurrence of the two is even more rare. The present study reported a case of gallbladder agenesis combined with congenital ASD. A 38-year-old male patient presented with a 3-year history of recurring upper right abdominal pain. The pain had no evident cause and was accompanied by dyspepsia and gasteremphraxis with indigestion. Several color Doppler ultrasonography scans revealed cholecystitis and gallbladder stones. A physical examination revealed cardiac murmur. A color Doppler ultrasonography of the heart was indicative of congenital heart disease. A corrective surgery for ASD was performed. Subsequently, a mini-incision cholecystectomy was performed as explorative surgery. A magnetic resonance cholangiopancreatography scan of the abdomen was performed in order to confirm the diagnosis of gallbladder disorder and cystic duct hypoplasia. A final definite diagnosis of gallbladder agenesis was confirmed. In conclusion, atrophy and gallbladder full of stones are frequently misdiagnosed, and establishing a definitive preoperative diagnosis is difficult. Awareness of this congenital malformation may assist physicians in distinguishing cases with unclear manifestation and avoiding unnecessary surgical interventions.


PubMed | Chinese Academy of Agricultural Sciences, Inner Mongolia International Mongolian Hospital, Southwest University of Science and Technology, Liaocheng University and Qingdao Agricultural University
Type: Journal Article | Journal: Genetics and molecular research : GMR | Year: 2016

Desmoglein 4 (DSG4) has an important role in the development of wool traits in domestic animals. The full-length DSG4 gene, which contains 3918 bp, a complete open-reading-frame, and encodes a 1040-amino acid protein, was amplified from Liaoning cashmere goat. The sequence was compared with that of DSG4 from other animals and the results show that the DSG4 coding region is consistent with interspecies conservation. Thirteen single-nucleotide polymorphisms (SNPs) were identified in a highly variable region of DSG4, and one SNP (M-1, G>T) was significantly correlated with white and black coat color in goat. Haplotype distribution of the highly variable region of DSG4 was assessed in 179 individuals from seven goat breeds to investigate its association with coat color and its differentiation among populations. However, the lack of a signature result indicates DGS4 haplotypes related with the color of goat coat.


Shuang M.,Tianjin University of Traditional Chinese Medicine | Shuang M.,Inner Mongolia International Mongolian Hospital | Zhao C.,Tianjin University of Traditional Chinese Medicine | Zhang L.,Tianjin University of Traditional Chinese Medicine | Shang H.-C.,Beijing University of Chinese Medicine
Chinese Journal of Evidence-Based Medicine | Year: 2016

Objective Using SYRCLE tool (the SYstematic Review Centre for Laboratory animal Experimentation) to evaluate the risk of bias of animal studies in stroke field published in Chinese journals, identify problems of these studies in design, implementation and measurement, in order to provide references for improving the quality of animal studies in China. Methods We searched databases including CBM, VIP, CNKI and WanFang Data from inception to December 31st, 2014. Two reviewers independently screened literature, extracted data, and assessed the risk of bias of included animal studies using SYRCLE tool developed by the National Centre for the Replacement, Refinement and Reduction of Animals in Research. Results A total of 582 studies were included. The assessment results showed that the number of reported items with “Low Risk” in SYRCLE, which have 22 items, reported in >50% of the 4 items and in <30% the 16 items in articles of animals experiments. More than 99% of the studies fulfilled the 3 items and more than 17% of the articles to meet the 10 items while less than 1% of the documents met the 17 items. The quality of studies increased excepted the period of 2010 to 2014. The methodological quality of animal experiments presented a trend of increasing and no significant differences were found in CSCD indexed or not. Conclusions The methodological quality of animal experiments of stroke is poor in China in terms of the selection bias, implementation bias, measurement bias, withdraw bias and reporting bias. © 2016 Editorial Board of Chin J Evid-based Med.


Arlud S.,Chinese Academy of Agricultural Sciences | Arlud S.,Inner Mongolia International Mongolian Hospital | E G.X.,Chongqing University of Science and Technology | Zeng S.C.,Chinese Academy of Agricultural Sciences
Genetics and Molecular Research | Year: 2016

Microsatellite DNA markers, which are assumed to drift, have been widely used to assess genetic diversity in all major domestic animal species. These markers provide insight into the arrival and dispersion history of a species, with regard to their content or management history. However, no direct evidence supports current standard microsatellite markers falling under this assumption. Therefore, the objective of this study was to investigate the effect and divergence of microsatellites under different types of selection on genetic patterns and population diversity. A total of 192 birds (Gallus gallus) from eight different geographic locations were investigated using 20 microsatellites that are classified into different groups by their selective effect (neutral, positive selection, and balancing selection) by the FDIST2 outlier test. The results showed that most polymorphisms were in the balancing selection marker group, the expected heterozygosity was 0.70, the observed heterozygosity was 0.65, and the mean number of alleles was 6.91. AMOVA revealed that the balancing group contributed the lowest amount of variance among groups, which was -0.60%, the highest variance contributed within the population being 92.28% in comparison with that of other groups. A similar pattern of population genetics was revealed following Slatkin linearized FST, principal component factor analysis, and population structure by Bayesian clustering. In conclusion, balancing selective markers offer high polymorphism for estimating genetic diversity but reduced genetic divergence between populations. © 2016 The Authors.


Yu H.,Inner Mongolia International Mongolian Hospital
Chinese Journal of Biologicals | Year: 2012

Objective: To construct the short hairpin RNA (shRNA) expression vector for human transforming growth factor-β1 (TGF-β1) gene and investigate its effect on invasion ability of human colon cancer SW480 cells. Methods: Two pairs of RNA interference sequences targeting TGF-β1 and one pair of negative control sequence were designed and synthesized, based on which recombinant plasmids pshRNA-TGF-β1a, pshRNA-TGF-β1b and pshRNA-TGF-β1c(negative control) were constructed and transfected into SW480 cells in mediation of Lipofectamine 2000. The transfected cells were observed by fluorescent microscopy, in which the transcription level of TGF-β1 mRNA was determined by RT-PCR, and the expression level of TGF-β1 protein by Western blot. The effect of TGF-β1 gene expression on invasion ability of SW480 cells was evaluated by Transwell test. Results: Restriction analysis and sequencing showed that all the recombinant plasmids were constructed correctly. As compared with those in blank control group, strong green fluorescence was observed in the cells transfected with the three recombinant plasmids; both mRNA transcription and protein expression levels of TGF-β1, as well as the invasion abilities of cells transfected with recombinant plasmids pshRNA-TGF-β1a and pshRNA-TGF-β1b decreased significantly (each P < 0.05). Conclusions: The recombinant shRNA expression vectors for TGF-β1 gene was constructed successfully, which effectively inhibited the expression of TGF-β1 gene in SW480 cells and decreased the invasion ability of the cells. It provided a novel idea for gene therapy of colon cancer.

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