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Yu H.,Inner Mongolia International Mongolian Hospital
Chinese Journal of Biologicals | Year: 2012

Objective: To construct the short hairpin RNA (shRNA) expression vector for human transforming growth factor-β1 (TGF-β1) gene and investigate its effect on invasion ability of human colon cancer SW480 cells. Methods: Two pairs of RNA interference sequences targeting TGF-β1 and one pair of negative control sequence were designed and synthesized, based on which recombinant plasmids pshRNA-TGF-β1a, pshRNA-TGF-β1b and pshRNA-TGF-β1c(negative control) were constructed and transfected into SW480 cells in mediation of Lipofectamine 2000. The transfected cells were observed by fluorescent microscopy, in which the transcription level of TGF-β1 mRNA was determined by RT-PCR, and the expression level of TGF-β1 protein by Western blot. The effect of TGF-β1 gene expression on invasion ability of SW480 cells was evaluated by Transwell test. Results: Restriction analysis and sequencing showed that all the recombinant plasmids were constructed correctly. As compared with those in blank control group, strong green fluorescence was observed in the cells transfected with the three recombinant plasmids; both mRNA transcription and protein expression levels of TGF-β1, as well as the invasion abilities of cells transfected with recombinant plasmids pshRNA-TGF-β1a and pshRNA-TGF-β1b decreased significantly (each P < 0.05). Conclusions: The recombinant shRNA expression vectors for TGF-β1 gene was constructed successfully, which effectively inhibited the expression of TGF-β1 gene in SW480 cells and decreased the invasion ability of the cells. It provided a novel idea for gene therapy of colon cancer. Source


Shang L.-J.,Inner Mongolia University | Wu Y.,Inner Mongolia University | Xu Y.-J.,Inner Mongolia International Mongolian Hospital
Chinese Journal of Tissue Engineering Research | Year: 2014

BACKGROUND: To maintain the long-term effect of full crown largely depends on the health of periodontal tissues. OBJECTIVE: To explore the effect of CAD/CAM zirconia all-ceramic crown restoration on the state of periodontal health. METHODS: Sixty-four abutments of 55 patients were randomly divided into two groups: the experimental group included 32 abutments of 29 patients which would be restored by CAD/CAM zirconia all-ceramic crowns; the controlled group included 32 abutments of 26 patients which would be restored by Ni-Cr alloy porcelain-fused-tometal restorations. The volume of gingival crevicular fluid and the levels of interleukin-6 and tumor necrosis factor-α in the two groups were examined at the pre-restoration and post-restoration stages. Meanwhile, periodontal clinical indicators, including sulcus bleeding index, probing depth, plaque index and attachment loss were recorded. RESULTS AND CONCLUSION: No difference in various indexes was found in the experimental group before and after restoration (P > 0.05). At 12 months after restoration, in the control group, the volume of gingival crevicular fluid, the levels of interleukin-6 and tumor necrosis factor-α, sulcus bleeding index, probing depth, and plaque index were all increased (P > 0.05); meanwhile, these indexes in the experimental group were significantly lower than those in the control group (P < 0.05). Experimental findings suggest that the CAD/CAM zirconia all-ceramic crown restoration is more favorable to the health of periodontal tissues. © 2014, Journal of Clinical Rehabilitative Tissue Engineering Research. All rights reserved. Source


Lan H.,Tokushima University | Nishitani H.,Tokushima University | Nishihara S.,Tokushima University | Ueno J.,Tokushima University | And 5 more authors.
Journal of Medical Investigation | Year: 2011

Purpose: The purpose of this study was to evaluate the usefulness of thick slab minimum intensity projection (MinIP) as a follow-up method in patients with pulmonary emphysema. This method was used to determine the presence or absence of changes over time in the lung field based on multi-detector-row CT (MDCT) data. Methods: Among pa- tients diagnosed with pulmonary emphysema who underwent 16-MDCT (slice thickness, 1 mm) twice at an interval of 6 months or more, 12 patients without changes in the lung field and 14 with clear changes in the lung field were selected as subjects. An image in- terpretation experiment was performed by five image interpreters. Pulmonary emphy- sema was followed up using two types of thick slab MinIP (thick slab MinIP 1 and 2) and multi-planar reformation (MPR), and the results of image interpretation were evaluated by receiver operating characteristic (ROC) analysis. In addition, the time required for image interpretation was compared among the three follow-up methods. Results: The area under the ROC curve (Az) was 0.794 for thick slab MinIP 1, 0.778 for the thick slab MinIP 2, and 0.759 for MPR, showing no significant differences among the three meth- ods. Individual differences in each item were significantly more marked for MPR than for thick slab MinIP. The time required for image interpretation was around 18 seconds for thick slab MinIP 1, 11 seconds for thick slab MinIP 2, and approximately 127 seconds for MPR, showing significant differences among the three methods. Conclusion: There were no significant differences in the results of image interpretation regarding the pres- ence or absence of changes in the lung fields between thick slab MinIP and MPR. How- ever, thick slab MinIP showed a shorter image interpretation time and smaller individual differences in the results among image interpreters than MPR, suggesting the usefulness of this method for determining the presence or absence of changes with time in the lung fields of patients with pulmonary emphysema. Source


Dalai N.,Jilin University | Cui H.,Inner Mongolia University | Yan M.,Inner Mongolia University | Rile G.,Inner Mongolia International Mongolian Hospital | And 2 more authors.
Genetics and Molecular Research | Year: 2014

Lifestyle, habits, diet, and genetics are all important factors associated with the prevalence of hypertension. Many association studies have been performed in the Chinese Han population, whereas data explaining the high prevalence of hypertension in the Mongolian population remain scarce. In the present study, we aimed to determine the factors associated with the development of essential hypertension in Mongolians. A total of 194 hypertensive cases and 201 controls from Dongwu County were enrolled in the study. Demographics, anthropometric and blood biochemical parameters, food intake, lifestyle, habits, education, occupation, and family history were recorded for each subject. Genotype and allele frequencies of six single nucleotide polymorphisms (SNPs) of the kallikrein 1 (KLK1) gene were also examined. Mean body mass index, waistline, hipline, blood sugar, blood urea nitrogen, creatinine, uric acid, total cholesterol, triglyceride, and low-density lipoprotein levels were all significantly higher in the hypertensive group (P < 0.01). Hypertensives consumed less milk, vegetables, and fruits, and had higher cigarette, alcohol, and salt intake (P < 0.05). There were also less regular physical exercisers and manual workers among the hypertensive group (P < 0.05). The mean inheritance rank of the hypertensive group was higher than that of controls (P < 0.05). There were no differences in the distribution of genotype and allele frequencies of the six SNPs between the hypertensive and control groups (P > 0.05). These results suggest that dietary history and habits have the most important influence on the development of essential hypertension in the Mongolian population. © FUNPEC-RP. Source


Liu Y.,Inner Mongolia University | Ao N.,Inner Mongolia International Mongolian Hospital | Zhao H.,Inner Mongolia University
Experimental and Therapeutic Medicine | Year: 2016

Congenital absence of gallbladder and atrial septal defect (ASD) are clinically rare congenital organ malformations, and the simultaneous occurrence of the two is even more rare. The present study reported a case of gallbladder agenesis combined with congenital ASD. A 38-year-old male patient presented with a 3-year history of recurring upper right abdominal pain. The pain had no evident cause and was accompanied by dyspepsia and gasteremphraxis with indigestion. Several color Doppler ultrasonography scans revealed cholecystitis and gallbladder stones. A physical examination revealed cardiac murmur. A color Doppler ultrasonography of the heart was indicative of congenital heart disease. A corrective surgery for ASD was performed. Subsequently, a mini-incision cholecystectomy was performed as explorative surgery. A magnetic resonance cholangiopancreatography scan of the abdomen was performed in order to confirm the diagnosis of gallbladder disorder and cystic duct hypoplasia. A final definite diagnosis of gallbladder agenesis was confirmed. In conclusion, atrophy and gallbladder full of stones are frequently misdiagnosed, and establishing a definitive preoperative diagnosis is difficult. Awareness of this congenital malformation may assist physicians in distinguishing cases with unclear manifestation and avoiding unnecessary surgical interventions. © 2016, Spandidos Publications. All rights reserved. Source

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