Rice R.H.,University of California at Davis |
Xia Y.,Inner Mongolia Center for Endemic Disease Control and Research |
Alvarado R.J.,University of California at Davis |
Phinney B.S.,University of California at Davis
Journal of Proteome Research | Year: 2010
Shotgun proteomic analysis of the human nail plate identified 144 proteins in samples from Causcasian volunteers. The 30 identified proteins solubilized by detergent and reducing agent, 90% of the total nail plate mass, were primarily keratins and keratin associated proteins. Keratins comprised a majority of the detergent-insoluble fraction as well, but numerous cytoplasmic, membrane, and junctional proteins and histones were also identified, indicating broad use by transglutaminases of available proteins as substrates for cross-linking. Two novel membrane proteins were identified, also found in the hair shaft, for which mRNAs were detected only at very low levels by real-time polymerase chain reaction in other tissues. Parallel analyses of nail samples from volunteers from Inner Mongolia, China gave essentially the same protein profiles. Comparison of the profiles of nail plate and hair shaft from the latter volunteers revealed extensive overlap of protein constituents. Analyses of samples from an arsenic-exposed population revealed few proteins whose levels were altered substantially but raised the possibility of detecting sensitive individuals in this way. © 2010 American Chemical Society.
Wang Y.,Inner Mongolia Agricultural University |
Li J.-Y.,Inner Mongolia Center for Endemic Disease Control and Research |
Han M.,Inner Mongolia Agricultural University |
Wang W.-L.,Inner Mongolia Agricultural University |
Li Y.-Z.,Inner Mongolia Agricultural University
Lipids in Health and Disease | Year: 2015
Abstract Background: The incidence of nonalcoholic fatty liver disease (NAFLD) has been increasing worldwide in parallel with the obesity epidemic. This study aims to investigate the effects of the total flavonoids in Stellera chamaejasme L. (TFSC) on the experimental NAFLD in high fat diet fed (HFD) rats. Methods: NAFLD model was induced in male Wistar rats by high-fat diet, and the rats in NAFLD group were randomized into NAFLD group (n = 20) and TFSC-treated group (n = 60). Both groups were given high-fat diet, and the normal group (n = 20) was given normal diet. In addition, the TFSC treated group was administered TFSC orally once a day at a low dose of 100 mg/kg (n = 20), medium dose of 200 mg/kg (n = 20), and high dose of 400 mg/kg (n = 20) for 6 weeks. Subsequently, the rats were sacrificed and body weight changes, lipid profiles in plasma and liver pathology were examined. The relative levels of fatty acid synthesis and β-oxidation gene expression in hepatic tissues were measured by quantitative real-time polymerase chain reaction (RT-PCR). Results: After the HFD administration for 4 weeks, the body weight,serum TC and TG levels in the rat of model group were significantly higher than in normal group (P < 0.05), and which Showed that the experimental NAFLD model was successfully established. While continual feeding with HFD deteriorated NAFLD and hyperlipidemia, and treatment with the different doses of TFSC effectively improved serum and liver lipid metabolism and liver function. A linear relationship between the dose of TFSC and blood lipid level was observed. The mRNA expression of hepatic acetyl-CoA carboxylase (ACC), fatty acid synthase (FAS), Leptin (LEP) and sterol regulatory element binding protein (SREBP)-1c as well as peroxisome proliferator-activated receptor (PPAR) -γ were significantly lower in high-dose group compared to the positive control group (P < 0.05). The hepatic mRNA expression of Cholesterol 7α-hydroxylase1 (CYP7A1), Carnitine palmitoyltransferase-1 (CPT1) and peroxisome proliferator-activated receptor (PPAR) -α were significantly higher in the high-dose group compared to the positive control group (P < 0.05). However, no difference was detected in the middle-dose group or the low-dose group compared to the positive control group (P > 0.05). Conclusion: TFSC treatment effectively improved NAFLD-related hyperlipidemia and inhibited liver steatosis in rats, and accompanied by modulating the expression of genes for regulating lipid metabolism. © 2015 Wang et al.
Zhang Z.-Z.,Inner Mongolia Center for Endemic Disease Control and Research
Chinese Journal of Endemiology | Year: 2013
Objective: To investigate the current status of iodine deficiency disorders (IDD) in Inner Mongolia Autonomous Region, residents iodine nutrition level and preventive measures being implemented, so as to master the background status of residents iodine nutrition before introduction of new concentration standards of iodized salt in the autonomous region; and to evaluate the effect of interventions implemented. Methods: According to stratified sampling with probability proportional to size(PPS), a sample of 30 units was extracted, and then a primary school was extracted from each sampling unit. From each primary school 40 students aged 8-10(male and female half and half) were checked thyroid volume though B-ultrasonic and iodine content of edible salt in these students' homes was quantitatively determined. Around the primary school, 3 villages were randomly selected, and then 5 pregnant and 5 lactating women were selected. Urinary iodine was measured by ammonium persulfate digestion-arsenic cerium catalysis spectrophotometry. Children urinary iodine level monitoring: In each county (city, district, flag) 1 village (town, district office) was randomly selected according to its sub-area positions in the north, the south, the east, the west and the center of the county; and then a village primary school (in the absence of village primary school, township central primary school was selected) was selected; from each school 20 children aged 8-10 were selected. A total of 100 urine samples were tested in each county. Results: Goiter rate of children aged 8-10 was 3.1%(37/1206); median urinary iodine level(UI) was 259.9 μg/L; the median of residents consumption of iodized salt was 30.2 mg/kg; the coverage rate of iodized salt was 99.3% (1194/1203); the consumption rate of iodized salt was 98.1% (1184/1203); the qualified rate of iodized salt was 98.8% (1180/1194); the rate of non-iodized salt was 0.7% (8/1203) and failure rate was 1.2%(15/1203). The number of urine sample was 6993; the median urinary iodine was 249.0 μg/L in children age 8-10, and UI level lower than 50.0 μg/L accounted for 1.50% (105/1993). Conclusions: After salt iodization as main comprehensive measures in prevention and control of iodine deficiency disorders, remarkable progress has been made in Inner Mongolia. The coverage rate of iodized salt, the consumption of iodized salt, goiter rate, and UI median all have reached the standard of elimination of iodine deficiency disorders. Children iodine nutrition level is in enough state, pregnant and lactating women iodine nutrition level is in suitable condition.
Guo Z.-W.,Inner Mongolia Center for Endemic Disease Control and Research
Chinese Journal of Endemiology | Year: 2013
Objective: To observe the effects of chronic arsenic exposure on estrogen receptor-binding fragment-associated gene 9 (Ebag9) and estrogen-responsive finger protein (efp) mRNA expression in female rat's myocardium. Methods: Fifty female Wistar rats were randomly divided into five groups according to arsenic (As2O3) concentrations in drinking-water: 0.00 (control), 0.05, 0.10, 0.20, 0.40 mg/L groups and RT-PCR was used to detect Ebag9 and efp mRNA expression of myocardium at the 32 weeks of experiment. Results: Ebag9 and efp mRNA expression levels in 0.00, 0.05, 0.10, 0.20, 0.40 mg/L groups were respectively as follows: 0.54 ± 0.14, 0.52 ± 0.10, 0.48 ± 0.24, 0.58 ± 0.13, 0.45 ± 0.19 and 0.85 ± 0.14, 0.86 ± 0.12, 0.87 ± 0.09, 0.99 ± 0.10, 0.86 ± 0.19. Compared to the control group, Ebag9 mRNA level of the 0.20 mg/L group was increased, and decreased in other groups, but the difference between two groups was not significant (all P > 0.05). Compared to control group, the efp mRNA level of 0.20 mg/L group increased significantly (P < 0.05), and showed increased tendency in other arsenic groups, but the difference between two groups was not significant (all P > 0.05). Conclusions: Ebag9 and efp mRNA expression have changed in myocardium of rats exposed to chronic arsenic. Arsenic may has endocrine disruptor effect to female rat's myocardium.
Liu J.,Inner Mongolia Center for Endemic Disease Control and Research
Chinese Journal of Endemiology | Year: 2012
Objective: To evaluation the intervention effects of hand hold hands method in brick tea borne fluorosis. Method: Four to six grade pupils were selected in brick tea borne fluorosis areas, and health education knowledge questionnaire investigation was conducted. Drinking tea related health behaviors were also surveyed by the method of questionnaire. Then health education lecture on brick tea borne fluorosis was carried out, and health education material and low fluoride brick tea were provided to the pupils, the students were also requested to tell their parents brick tea related knowledge and supervise them to get rid of harmful drinking tea behavior, and cultivate healthy lifestyle. One year later pupils ' health knowledge questionnaire and parents health related behavior questionnaire were carried out once again. The knowledge awareness rates of students before and after the intervention were compared. Health related behavior change rates of the parent between different ages and education levels were compared, and the implementation rates of different brick tea related behaviors before and after the intervention were compared. Results: After 1 year of health education, the student's knowledge awareness rate on brick tea fluorosis was [94.3%(33/35)], compared with the rate before intervertion[14.3%(5/35)] increased significantly (χ2 = 46.111, P < 0.01). The overall behavior change rate of health related behavior was 68.6%. After the intervention, the implementation rate of whether drinking tea, drinking overnight tea, drinking boiled for a long time tea decreased(P< 0.01). But the behavior implementation rates of drinking tea with milk, drinking and buying low fluoride brick tea significandy increased(P < 0.05). Implementation rate of whether drinking brick tea did not change significantly(P > 0.05). Conclusions: Through hand hold hands health education intervention, the knowledge awareness of students on brick tea fluorosis increases, and their parents can actively change bad tea drinking habits, and cultivate healthy lifestyle.
Guo H.,Inner Mongolia Center for Endemic Disease Control and Research
Wei sheng yan jiu = Journal of hygiene research | Year: 2011
By measure the changes of serumal estadiol and progesterone levels of kunming female mice, which are chronically exposed to different concentrations of arsenic trioxide, discuss the estrogen-like effects of arsenic. Select the Kunming female mice exposed to different drinking water of arsenic trioxide (0, 0.05, 0.10, 0.20, 0.40 microg/ml) for 20 weeks as the research subjects in this study. Use the method of radioimmunology to measure concentrations of serumal estadiol and progesterone of female adult mice. Compared with the control group, there exists the changes of the concentrations of the serumal estadiol and progesterone of Km female adult mice, which are chronically exposed to the arsenic. Compared to the control group, the differences of the concentrations of the serumal estadiol in the 0.05 microg/ml group and 0.10 microg/ml group have no statistical significance (P > 0.05), the concentrations of estadiol in the 0.20 microg/ml group (P < 0.01) and 0.40 microg/ ml group (P < 0.05) decrease. Compared to the control group, the differences of the concentrations of the serumal progesterone in the 0.05 microg/ml group, 0.20 microg/ml group and 0.40 microg/ml group have no statistical significance (P > 0.05), the concentration of serumal progesterone in the 0.10 microg/ml group (P < 0.01) decrease. Chronically arsenic exposure can interfere the levels of serumal hormones. Arsenic has the estrogen-like effects.
Guo Z.,Inner Mongolia Center for Endemic Disease Control and Research
Chinese Journal of Endemiology | Year: 2014
Objective To observe the effects of chronic arsenic exposure on mRNA expression of estrogen receptor-binding fragment-associated gene 9(Ebag9) and estrogen-responsive finger protein efp in uterus and ovary of female rats. Methods Fifty female Wistar rats were randomly divided into five groups according to arsenic (As2O3) concentrations given through drinking-water: 0.00 (control), 0.05 , 0.10 , 0.20 , 0.40 mg/L arsenic exposure groups and real-time RCR (RT-PCR) was used to detect the mRNA expression of Ebag9 and efp in uterus and ovary tissue at the 31 weeks of experiment. Results The mRNA expression levels of Ebag9 and efp of the 0.00 , 0.05, 0.10, 0.20, 0.40 mg/L arsenic exposure groups were respectively as follows: 0.761 ± 0.178, 0.521 ± 0.130, 0.544 ± 0.035 , 0.525 ± 0.198, 0.498 ± 0.240 and 0.795 ± 0.171, 0.874 ± 0.077, 0.797 ± 0.066 , 0.796 ± 0.040, 0.832 ± 0.096. Compared with control group, a decreased tendency was observed in Ebag9 mRNA level (with P value 0.055 in 0.40 mg/L arsenic exposure group) and increased tendency in efp mRNA level in experimental groups (all P > 0.05). The mRNA expression levels of Ebag9 and efp in ovary of the five groups were by turns; 0.702 ± 0.484 , 0.719 ± 0.336, 0.693 ± 0.095 , 0.706 ± 0.055, 0.728 ± 0.073 and 0.924 ± 0.061, 1.009 ± 0.034, 0.930 ± 0.085, 0.929 ± 0.068, 1.012 ± 0.101. Compared with control group, the expression level of Ebag9 mRNA showed a increased tendency in 0.05 , 0.20, 0.40 mg/L arsenic exposure groups(all P > 0.05). The efp mRNA level increased in experimental groups, with significant difference in 0.05 , 0.40 mg/L groups (all P < 0.05). Conclusions The expression of efp mRNA has changed in ovary of female rats exposed to chronic arsenic. Arsenic may act as an environmental endocrine disruptor to exert its effect.
Mo J.,University of North Carolina at Chapel Hill |
Xia Y.,Inner Mongolia Center for Endemic Disease Control and Research |
Wade T.J.,U.S. Environmental Protection Agency |
DeMarini D.M.,U.S. Environmental Protection Agency |
And 2 more authors.
International Journal of Environmental Research and Public Health | Year: 2011
Chronic arsenic exposure results in higher risk of skin, lung, and bladder cancer, as well as cardiovascular disease and diabetes. The purpose of this study was to investigate the effects on expression of selected genes in the blood lymphocytes from 159 people exposed chronically to arsenic in their drinking water using a novel RT-PCR TaqMan low-density array (TLDA). We found that expression of tumor necrosis factor-α (TNF-α), which activates both inflammation and NF-κB-dependent survival pathways, was strongly associated with water and urinary arsenic levels. Expression of KCNA5, which encodes a potassium ion channel protein, was positively associated with water and toe nail arsenic levels. Expression of 2 and 11 genes were positively associated with nail and urinary arsenic, respectively. Because arsenic exposure has been reported to be associated with long QT intervals and vascular disease in humans, we also used this TLDA for analysis of gene expression in human cardiomyocytes exposed to arsenic in vitro. Expression of the ion-channel genes CACNA1, KCNH2, KCNQ1 and KCNE1 were down-regulated by 1-uM arsenic. Alteration of some common pathways, including those involved in oxidative stress, inflammatory signaling, and ion-channel function, may underlay the seemingly disparate array of arsenic-associated diseases, such as cancer, cardiovascular disease, and diabetes. © 2011 by the authors; licensee MDPI, Basel, Switzerland.
PubMed | Inner Mongolia Center for Endemic Disease Control and Research
Type: Journal Article | Journal: Zhonghua xin xue guan bing za zhi | Year: 2016
To investigate the relationship between blood aryl hydrocarbon receptor(AhR)mRNA and cytochrome P450(CYP)1A1 mRNA expression and corrected QT interval among residents exposed to arsenic via drinking water.Arsenic exposure area in Bayannaoer city of Nei Monggol Autonomous Region was selected as the survey point, and the residents living more than 10 years in this area were investigated from December 2012 to January 2015.A total of 233 residents were divided into four groups according to drinking water arsenic concentration (ranged from 0.8 to 824.7 g/L): control group (drinking water arsenic concentration <10.0 g/L, n=55), low exposure group (drinking water arsenic concentration 10.0-99.9 g/L, n=47), middle exposure group (drinking water arsenic concentration 100.0-199.9 g/L, n=45), high exposure group (drinking water arsenic concentration 200.0 g/L, n=86). Epidemiological investigation was performed.Real-time PCR technology was used to detect the expression levels of blood AhR mRNA and CYP1A1 mRNA, and the relationship between expression levels of blood AhR mRNA and CYP1A1 mRNA and corrected QT interval was analyzed.(1) Blood AhR mRNA and CYP1A1 mRNA expression levels were similar among control group, low exposure group and middle exposure group (all P>0.05) while blood AhR mRNA (3.1810(-3)(2.4210(-3), 4.4510(-3)) vs.2.3010(-3)(1.5310(-3), 3.2010(-3)), P<0.05) and CYP1A1 mRNA (1.5810(-3)(0.8010(-3), 2.7310(-3))vs.1.0010(-3)(0.5910(-3), 2.0910(-3)), P<0.05) expression levels were significantly higher in high dose group than in control group.(2) AhR mRNA expression level was similar between residents with longer corrected QT interval and residents with normal corrected QT interval (2.8910(-3)(1.9010(-3), 3.7110(-3)) vs.2.5810(-3)(1.8510(-3), 3.5710(-3)), P>0.05). CYP1A1 mRNA expression level was significantly higher in residents with longer corrected QT interval than in residents with normal corrected QT interval (1.5010(-3)(0.7810(-3), 2.6310(-3)) vs.1.1610(-3)(0.6410(-3), 2.3610(-3)), P<0.05). (3) AhR mRNA expression level was similar between residents with sinus tachycardia or bradycardia or normal heart rate(2.8910(-3)(1.7110(-3), 4.4510(-3)) vs.2.6410(-3)(1.8610(-3), 3.5210(-3)), P>0.05). CYP1A1 mRNA expression level was significantly higher in residents with sinus tachycardia or bradycardia than in residents with normal heart rate (1.4710(-3)(0.8710(-3), 2.7710(-3)) vs.1.2410(-3)(0.6410(-3), 2.3110(-3)), P<0.05).Long-term exposure to arsenic is associated with upregulated blood AhRmRNA and CYP1A1 mRNA expression.Blood CYP1A1 mRNA expression, but not AhRmRNA expression, is associated with prolonged corrected QT interval.
PubMed | Inner Mongolia Center for Endemic Disease Control and Research and CAS Institute of Geographical Sciences and Natural Resources Research
Type: Journal Article | Journal: Biological trace element research | Year: 2016
More than 0.3 million individuals are subject to chronic exposure to arsenic via their drinking water in Inner Mongolia, China. To determine arsenic methylation capacity profiles for such individuals, concentrations of urinary arsenic metabolites were measured for 548 subjects using high-performance liquid chromatography and a hydride generator combined with inductively coupled plasma-mass spectrometry. Mean urinary concentrations of dimethylarsonic acid (DMA), monomethylarsonic acid (MMA), inorganic arsenic (iAs), and total arsenic (TAs) were 200.50, 46.71, 52.96, and 300.17 g/L, respectively. The %iAs, %DMA, and %MMA were 15.98, 69.72, and 14.29%. Mean urinary %iAs and %MMA were higher in males, while urinary %DMA was higher in females. There was a strong positive correlation between %iAs and %MMA, with negative correlations between %iAs and %DMA, and %iAs and %MMA. In addition, %iAs and %MMA were positively associated with total arsenic in drinking water (WAs), while %DMA was negatively related with WAs. Regression analysis indicated that the primary methylation index (PMI) and secondary methylation index (SMI) generally decreased with increasing WAs. Females had a higher arsenic methylation capacity compared to males. Younger subjects had lower primary arsenic methylation capacity. However, the secondary arsenic methylation capacity was hardly affected by age. Moreover, both primary and secondary arsenic methylation capacities were negatively related to WAs.