Inner Mongolia Academy of Agricultural & Animal Husbandry science

Hohhot, China

Inner Mongolia Academy of Agricultural & Animal Husbandry science

Hohhot, China

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PubMed | Inner Mongolia Academy of Agricultural & Animal Husbandry science and Agricultural University of Hebei
Type: Journal Article | Journal: Genetics and molecular research : GMR | Year: 2015

Poplar-cotton agro-ecosystems are the main agricultural planting modes of cotton fields in China. With increasing acres devoted to transgenic insect-resistant poplar and transgenic insect-resistant cotton, studies examining the effects of transgenic plants on target and non-target insects become increasingly important. We systematically surveyed populations of both target pests and non-target insects for 4 different combinations of poplar-cotton eco-systems over 3 years. Transgenic Bt cotton strongly resisted the target insects Fall webworm moth [Hyphantria cunea (Drury)], Sylepta derogata Fabrieius, and American bollworm (Heliothis armigera), but no clear impact on non-target insect cotton aphids (Aphis gossypii). Importantly, intercrops containing transgenic Pb29 poplar significantly increased the inhibitory effects of Bt cotton on Fall webworm moth in ecosystem IV. Highly resistant Pb29 poplar reduced populations of the target pests Grnsonoma minutara Hubner and non-target insect poplar leaf aphid (Chaitophorus po-pulialbae), while Fall webworm moth populations were unaffected. We determined the effects of Bt toxin from transgenic poplar and cotton on target and non-target pests in different ecosystems of cotton-poplar intercrops and identified the synergistic effects of such combinations toward both target and non-target insects.


Zhang D.J.,China Agricultural University | Zhang D.J.,Inner Mongolia University | Lu Z.Y.,Inner Mongolia Academy of Agricultural & Animal Husbandry science | Liu J.X.,China Agricultural University | And 2 more authors.
Genetics and Molecular Research | Year: 2015

Poplar-cotton agro-ecosystems are the main agricultural planting modes of plain cotton fields in China. Here, we performed a systematic survey of the diversity and population of arthropod communities in four different combination of poplar-cotton eco-systems, including I) non-transgenic poplar and non-transgenic cotton fields; II) non-transgenic poplar and transgenic cotton fields [Bacillus thuringiensis (Bt) cotton]; III) Bt transgenic poplar (high insect resistant strain Pb29) and non-transgenic cotton; and IV) transgenic poplar and transgenic cotton fields, over a period of 3 years. Based on the statistical methods used to investigate community ecology, the effects of transgenic ecosystems on the whole structure of the arthropod community, on the structure of arthropods in the nutritive layer, and on the similarity of arthropod communities were evaluated. The main results were as follows: the transgenic poplar-cotton ecosystem has a stronger inhibitory effect on insect pests and has no impact on the structure of the arthropod community, and therefore, maintains the diversity of the arthropod community. The character index of the community indicated that the structure of the arthropod community of the transgenic poplar-cotton ecosystem was better than that of the poplar-cotton ecosystem, and that system IV had the best structure. As for the abundance of nutritional classes, the transgenic poplar-cotton ecosystem was also better than that of the non-transgenic poplar-cotton ecosystem. The cluster analysis and similarity of arthropod communities between the four different transgenic poplar-cotton ecosystems illustrated that the structure of the arthropod community excelled in the small sample of the transgenic poplar-cotton ecosystems. © FUNPEC-RP.


Bai C.-L.,Inner Mongolia Agricultural University | Bai C.-L.,Inner Mongolia Academy of Agricultural & Animal Husbandry science | Liu Y.-Z.,Inner Mongolia Academy of Agricultural & Animal Husbandry science | Wang M.-J.,Inner Mongolia Agricultural University | And 2 more authors.
Acta Prataculturae Sinica | Year: 2016

Knowledge of herbage nitrogen (N) and phosphorus (P) in plants is fundamental to the study of absorption and utilization of nutrients by the plants. In order to better understand limiting factors and plant adaption strategies to environmental factors in the grassland ecosystem, water and N enhancement experiments were carried out in a Stipa breviflora steppe. Four treatments were set up: nitrogen addition (ND), water addition (WD), nitrogen and water addition (NWD) and control (CK) with five replicates in a free grazed paddock in a S. breviflora desert steppe, Inner Mongolia. The S. breviflora leaf N and P concentrations and their stoichiometry under different treatments were analyzed. It was found that S. breviflora enhanced adaptability to barren habitats by increasing leaf N content. Uptake of N by S. breviflora was limited by low soil N and water contents. The absolute leaf N and P contents and the N:P ratio were more sensitive to the environmental addition of N and water in mid season than in late season. The availability of environmental N greatly influenced the leaf N and P contents and N:P ratio in mid season and the N:P ratio in late season when plants were withering. However, the availability of water only affected the leaf N and P content in mid season and not in late season. Copyright © ACTA PRATACULTURAE SINICA.


Nie L.,Inner Mongolia Agricultural University | Nie L.,Inner Mongolia Academy of Agricultural Animal Husbandry science | Wang R.,Inner Mongolia Agricultural University | Xia Y.,Hong Kong Baptist University | Li G.,Inner Mongolia Agricultural University
Russian Journal of Plant Physiology | Year: 2015

Growing evidences indicate that calcium-dependent protein kinases (CDPKs) are involved in many aspects of plant’s growth, development and responses to biotic and abiotic stresses. However, the biological roles of most CDPKs remain unclear. Here we report that CDPK1 is induced by infection with both virulent and avirulent Pseudomonas syringae strains. To understand the biological function of CDPK1, we analyzed phenotypes of the T-DNA insertion line (cpk10) which did not express the CDPK1 gene. The cpk10 mutant exhibited deduced expression of the defense-related genes PR1, PR2, and AIG1 following inoculation with the avirulent pathogen Pst AvrRpt2. In addition, the CDPK1 gene was found to be expressed in guard cells and the cpk10 mutant showed alteration in stomata closure after Pst DC3000 infection. We also found that expression of CDPK1 is induced by plant hormones salicylic acid, methyl jasmonate, and abscisic acid. These results indicate that CDPK1 may be involved in plant defense response. © 2015, Pleiades Publishing, Ltd.


PubMed | Inner Mongolia Academy of Agricultural Animal Husbandry science and Inner Mongolia Agricultural University
Type: Journal Article | Journal: Yi chuan = Hereditas | Year: 2014

A genetic linkage map of sunflower was constructed by combined applying the SSR and AFLP markers using 187 F5:6 individuals of recombinant inbred lines (RILs) which derived from the cross between Helianthus annuus K55 and Helianthus annuus K58 through single-seed descent (SSD). Using 78 pairs of SSR primers and 48 pairs of AFLP primer, 341 and 1119 bands were amplified, respectively. Among these 1460 bands, 557 bands (39.52%) were polymorphic, including 184 bands by SSR markers and 393 bands by AFLP markers. In the group of these polymorphic bands, 84 bands from SSR markers and 108 bands from AFLP markers showed the genetic distortion (P = 0.05). A total of 192 segregation distortion markers were obtained in this study. By using the JoinMap 4.0 software to do the linkage analysis, a genetic linkage map was established with length of 2759.4 cM, consisted of 17 linkage groups, and comprised of 495 polymorphic molecular markers including 170 segregation distortion markers. The mean marker interval distance is 5.57 cM between markers. In addition, the number of markers in the linkage groups varied from 5 to 72, and the length of linkage groups were from 68.88 cM to 250.17 cM. The genetic map developed in the present study could be used for QTL mapping and gene cloning of sunflower important genes.


Cheng D.,Harbin Institute of Technology | Cui J.,Harbin Institute of Technology | Niu J.,Harbin Institute of Technology | Dai C.,Harbin Institute of Technology | Bai C.,Inner Mongolia Academy of Agricultural Animal Husbandry science
Harbin Gongye Daxue Xuebao/Journal of Harbin Institute of Technology | Year: 2014

To explore the molecular genetic mechanisms of cytoplasmic male sterility in sugar beet, the differential proteins between the CMS line and its maintainer line were studied. Immobilized pH gradient two-dimensional gel electrophoresis (2-DE) technique and MALDI-TOF-MS were used to identify differentially expressed proteins in the three pollen development stages(stamen primordium differentiation stage, metaphase and uninucleate stage) between the Owen sugar beet male-sterile line(DY5-CMS)and its maintainer line(DY5-O). Six distinct proteins were identified in the stamen primordium differentiation stage and four distinct proteins were identified in the metaphase. In addition, all those proteins were related to the energy and respiratory metabolism. It was inferred that cytoplasmic male sterility of sugar beet might take place at the prophase of pollen development(contain stamen primordium differentiation stage and metaphase). The over-expression of energy and respiratory metabolism related proteins leads to metabolism disorder. Six distinct proteins are identified in the uninucleate stage of pollen development, and most of the proteins are related to photosynthesis of plants. It is speculated that the male sterility trait has formed at the late stages of pollen development and male sterility leads to the decreasing of photosynthetic. ©, 2014, Harbin Gongye Daxue Xuebao/Journal of Harbin Institute of Technology. All right reserved.


PubMed | Chinese Academy of Sciences, Inner Mongolia Academy of Agricultural & Animal Husbandry science and CAS Kunming Institute of Zoology
Type: Journal Article | Journal: PloS one | Year: 2014

Stimulated by retinoic acid 6 (STRA6) is the receptor for retinol binding protein and is relevant for the transport of retinol to specific sites such as the eye. The adaptive evolution mechanism that vertebrates have occupied nearly every habitat available on earth and adopted various lifestyles associated with different light conditions and visual challenges, as well as their role in development and adaptation is thus far unknown. In this work, we have investigated different aspects of vertebrate STRA6 evolution and used molecular evolutionary analyses to detect evidence of vertebrate adaptation to the lightless habitat. Free-ratio model revealed significant rate shifts immediately after the species divergence. The amino acid sites detected to be under positive selection are within the extracellular loops of STRA6 protein. Branch-site model A test revealed that STRA6 has undergone positive selection in the different phyla of mammalian except for the branch of rodent. The results suggest that interactions between different light environments and host may be driving adaptive change in STRA6 by competition between species. In support of this, we found that altered functional constraints may take place at some amino acid residues after speciation. We suggest that STRA6 has undergone adaptive evolution in different branch of vertebrate relation to habitat environment.


PubMed | Inner Mongolia Academy of Agricultural & Animal Husbandry science and Inner Mongolia University of Technology
Type: Journal Article | Journal: BMC genomics | Year: 2017

Sunflower Verticillium wilt (SVW) is a vascular disease caused by root infection with Verticillium dahliae (V. dahlia). It is a serious threat to the yield and quality of sunflower. However, chemical and agronomic measures for controlling this disease are not effective. The selection of more resistant genotypes is a desirable strategy to reduce contamination. A deeper knowledge of the molecular mechanisms and genetic basis underlying sunflower Verticillium wilt is necessary to accelerate breeding progress.An RNA-Seq approach was used to perform global transcriptome profiling on the roots of resistant (S18) and susceptible (P77) sunflower genotypes infected with V. dahlia. Different pairwise transcriptome comparisons were examined over a time course (6, 12 and 24h, and 2, 3, 5 and 10 d post inoculation). In RD, SD and D datasets, 1231 genes were associated with SVW resistance in a genotype-common transcriptional pattern. Moreover, 759 and 511 genes were directly related to SVW resistance in the resistant and susceptible genotypes, respectively, in a genotype-specific transcriptional pattern. Most of the genes were demonstrated to participate in plant defense responses; these genes included peroxidase (POD), glutathione peroxidase, aquaporin PIP, chitinase, L-ascorbate oxidase, and LRR receptors. For the up-regulated genotype-specific differentially expressed genes (DEGs) in the resistant genotype, higher average fold-changes were observed in the resistant genotype compared to those in the susceptible genotype. An inverse effect was observed in the down-regulated genotype-specific DEGs in the resistant genotype. KEGG analyses showed that 98, 112 and 52 genes were classified into plant hormone signal transduction, plant-pathogen interaction and flavonoid biosynthesis categories, respectively. Many of these genes, such as CNGC, RBOH, FLS2, JAZ, MYC2 NPR1 and TGA, regulate crucial points in defense-related pathway and may contribute to V. dahliae resistance in sunflower.The transcriptome profiling results provided a clearer understanding of the transcripts associated with the crosstalk between sunflower and V. dahliae. The results identified several differentially expressed unigenes involved in the hyper sensitive response (HR) and the salicylic acid (SA)/jasmonic acid (JA)-mediated signal transduction pathway for resistance against V. dahliae. These results are useful for screening resistant sunflower genotypes.


PubMed | Inner Mongolia Academy of Agricultural & Animal Husbandry science and Agricultural University of Hebei
Type: Journal Article | Journal: Genetics and molecular research : GMR | Year: 2015

Poplar-cotton agro-ecosystems are the main agricultural planting modes of plain cotton fields in China. Here, we performed a systematic survey of the diversity and population of arthropod communities in four different combination of poplar-cotton eco-systems, including I) non-transgenic poplar and non-transgenic cotton fields; II) non-transgenic poplar and transgenic cotton fields [Bacillus thuringiensis (Bt) cotton]; III) Bt transgenic poplar (high insect resistant strain Pb29) and non-transgenic cotton; and IV) transgenic poplar and transgenic cotton fields, over a period of 3 years. Based on the statistical methods used to investigate community ecology, the effects of transgenic ecosystems on the whole structure of the arthropod community, on the structure of arthropods in the nutritive layer, and on the similarity of arthropod communities were evaluated. The main results were as follows: the transgenic poplar-cotton ecosystem has a stronger inhibitory effect on insect pests and has no impact on the structure of the arthropod community, and therefore, maintains the diversity of the arthropod community. The character index of the community indicated that the structure of the arthropod community of the transgenic poplar-cotton ecosystem was better than that of the poplar-cotton ecosystem, and that system IV had the best structure. As for the abundance of nutritional classes, the transgenic poplar-cotton ecosystem was also better than that of the non-transgenic poplar-cotton ecosystem. The cluster analysis and similarity of arthropod communities between the four different transgenic poplar-cotton ecosystems illustrated that the structure of the arthropod community excelled in the small sample of the transgenic poplar-cotton ecosystems.


Hao L.,Inner Mongolia Academy of Agricultural & Animal Husbandry science | Song P.,Inner Mongolia Academy of Agricultural & Animal Husbandry science | Huangfu H.,Inner Mongolia Academy of Agricultural & Animal Husbandry science | Li Z.,Inner Mongolia Academy of Agricultural & Animal Husbandry science
Phytoparasitica | Year: 2015

Phoma stem canker (blackleg), caused by the fungi Leptosphaeria maculans and L. biglobosa, is the most devastating disease in oilseed rape (canola) production worldwide. In this study, 84 Leptosphaeria isolates were collected in China from rapeseed (Brassica napus) plants with blackleg symptoms and identified using multiplex PCR and rDNA-ITS sequence analysis. Results showed that all these isolates are L. biglobosa and no L. maculans was detected. A total of 99 isolates was analyzed, including 15 other L. biglobosa isolates from the UK, Canada and Poland using rDNA-ITS sequences, and all isolates were found to belong to the L. biglobosa 'brassicae' subclade. The genetic variation of these 99 isolates was tested using ISSR (inter simple sequence repeats) markers using 24 polymorphic primers pairs that generated 241 bands by ISSR-PCR amplification. A dendrogram based on weighted pair group mean analysis of these bands revealed that L. biglobosa isolates from China clustered into different groups from those obtained from Canada and Europe. Furthermore, Chinese isolates from seven provinces, except those from Jiangsu, could be placed within the same cluster, based on geographical location. Nei’s gene diversity ranged from 0.25 in Jiangsu to 0.11 in Sichuan, and Shannon’s information index from 0.16 to 0.37. Genetic identity ranged from 0.784 to 0.834 when isolates from China were compared with those from Canada and the UK, respectively. Analysis of gene flow among populations (Nm) indicated that almost no gene exchange has occurred between L. biglobosa in China and the populations of the fungus from the UK or Canada. © 2014, Springer Science+Business Media Dordrecht.

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