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Jia J.,CAS Beijing Institute of Geographic Sciences and Nature Resources Research | Jia J.,University of Chinese Academy of Sciences | Dong Y.,CAS Beijing Institute of Geographic Sciences and Nature Resources Research | Qi Y.,CAS Beijing Institute of Geographic Sciences and Nature Resources Research | And 13 more authors.
Journal of Forestry Research | Year: 2015

Global change will lead to increases in regional precipitation and nitrogen (N) deposition in the semi-arid grasslands of northern China. We investigated the responses of vegetation carbon (C) pools to simulated precipitation and N deposition increases through field experiments in a typical steppe in Inner Mongolia. The treatments included NH4NO3 addition at concentrations of 0 (CK), 5 (LN, low nitrogen), 10 (middle nitrogen, MN), and 20 (HN, high nitrogen) (g m−2 a−1) with and without water. After three consecutive years of treatment, from 2010 to 2012, water addition did not significantly change the size of the total vegetation C pools, but it significantly decreased the ratio of root:shoot (R:S) (P = 0.05) relative to controls. By contrast, N addition significantly increased the total vegetation C pools. The C pools in the LN, MN and HN treatments increased by 22, 39 and 44 %, respectively. MN produced the largest effect among the N concentrations, although differences between N-added treatments were not significant (P > 0.05). N addition significantly reduced the ratio of root:shoot (R:S) (P = 0.03). However, there were no significant interactive effects of water and N addition on the vegetation C pools. © 2015 Northeast Forestry University and Springer-Verlag Berlin Heidelberg

Cheng D.,Harbin Institute of Technology | Cui J.,Harbin Institute of Technology | Niu J.,Harbin Institute of Technology | Dai C.,Harbin Institute of Technology | Bai C.,Inner Mongolia Academy of Agricultural & Animal Husbandry science
Harbin Gongye Daxue Xuebao/Journal of Harbin Institute of Technology | Year: 2014

To explore the molecular genetic mechanisms of cytoplasmic male sterility in sugar beet, the differential proteins between the CMS line and its maintainer line were studied. Immobilized pH gradient two-dimensional gel electrophoresis (2-DE) technique and MALDI-TOF-MS were used to identify differentially expressed proteins in the three pollen development stages(stamen primordium differentiation stage, metaphase and uninucleate stage) between the Owen sugar beet male-sterile line(DY5-CMS)and its maintainer line(DY5-O). Six distinct proteins were identified in the stamen primordium differentiation stage and four distinct proteins were identified in the metaphase. In addition, all those proteins were related to the energy and respiratory metabolism. It was inferred that cytoplasmic male sterility of sugar beet might take place at the prophase of pollen development(contain stamen primordium differentiation stage and metaphase). The over-expression of energy and respiratory metabolism related proteins leads to metabolism disorder. Six distinct proteins are identified in the uninucleate stage of pollen development, and most of the proteins are related to photosynthesis of plants. It is speculated that the male sterility trait has formed at the late stages of pollen development and male sterility leads to the decreasing of photosynthetic. ©, 2014, Harbin Gongye Daxue Xuebao/Journal of Harbin Institute of Technology. All right reserved.

Nie L.,Inner Mongolia Agricultural University | Nie L.,Inner Mongolia Academy of Agricultural & Animal Husbandry science | Wang R.,Inner Mongolia Agricultural University | Xia Y.,Hong Kong Baptist University | Li G.,Inner Mongolia Agricultural University
Russian Journal of Plant Physiology | Year: 2015

Growing evidences indicate that calcium-dependent protein kinases (CDPKs) are involved in many aspects of plant’s growth, development and responses to biotic and abiotic stresses. However, the biological roles of most CDPKs remain unclear. Here we report that CDPK1 is induced by infection with both virulent and avirulent Pseudomonas syringae strains. To understand the biological function of CDPK1, we analyzed phenotypes of the T-DNA insertion line (cpk10) which did not express the CDPK1 gene. The cpk10 mutant exhibited deduced expression of the defense-related genes PR1, PR2, and AIG1 following inoculation with the avirulent pathogen Pst AvrRpt2. In addition, the CDPK1 gene was found to be expressed in guard cells and the cpk10 mutant showed alteration in stomata closure after Pst DC3000 infection. We also found that expression of CDPK1 is induced by plant hormones salicylic acid, methyl jasmonate, and abscisic acid. These results indicate that CDPK1 may be involved in plant defense response. © 2015, Pleiades Publishing, Ltd.

Zhang Y.,Inner Mongolia Agricultural University | Yu H.,Inner Mongolia Academy of Agricultural & Animal Husbandry science | Hou J.,Inner Mongolia Agricultural University | Li S.,Inner Mongolia Academy of Agricultural & Animal Husbandry science | And 2 more authors.
Yi chuan = Hereditas / Zhongguo yi chuan xue hui bian ji | Year: 2014

A genetic linkage map of sunflower was constructed by combined applying the SSR and AFLP markers using 187 F5:6 individuals of recombinant inbred lines (RILs) which derived from the cross between Helianthus annuus K55 and Helianthus annuus K58 through single-seed descent (SSD). Using 78 pairs of SSR primers and 48 pairs of AFLP primer, 341 and 1119 bands were amplified, respectively. Among these 1460 bands, 557 bands (39.52%) were polymorphic, including 184 bands by SSR markers and 393 bands by AFLP markers. In the group of these polymorphic bands, 84 bands from SSR markers and 108 bands from AFLP markers showed the genetic distortion (P = 0.05). A total of 192 segregation distortion markers were obtained in this study. By using the JoinMap 4.0 software to do the linkage analysis, a genetic linkage map was established with length of 2759.4 cM, consisted of 17 linkage groups, and comprised of 495 polymorphic molecular markers including 170 segregation distortion markers. The mean marker interval distance is 5.57 cM between markers. In addition, the number of markers in the linkage groups varied from 5 to 72, and the length of linkage groups were from 68.88 cM to 250.17 cM. The genetic map developed in the present study could be used for QTL mapping and gene cloning of sunflower important genes.

Hao L.,Inner Mongolia Academy of Agricultural & Animal Husbandry science | Song P.,Inner Mongolia Academy of Agricultural & Animal Husbandry science | Huangfu H.,Inner Mongolia Academy of Agricultural & Animal Husbandry science | Li Z.,Inner Mongolia Academy of Agricultural & Animal Husbandry science
Phytoparasitica | Year: 2015

Phoma stem canker (blackleg), caused by the fungi Leptosphaeria maculans and L. biglobosa, is the most devastating disease in oilseed rape (canola) production worldwide. In this study, 84 Leptosphaeria isolates were collected in China from rapeseed (Brassica napus) plants with blackleg symptoms and identified using multiplex PCR and rDNA-ITS sequence analysis. Results showed that all these isolates are L. biglobosa and no L. maculans was detected. A total of 99 isolates was analyzed, including 15 other L. biglobosa isolates from the UK, Canada and Poland using rDNA-ITS sequences, and all isolates were found to belong to the L. biglobosa 'brassicae' subclade. The genetic variation of these 99 isolates was tested using ISSR (inter simple sequence repeats) markers using 24 polymorphic primers pairs that generated 241 bands by ISSR-PCR amplification. A dendrogram based on weighted pair group mean analysis of these bands revealed that L. biglobosa isolates from China clustered into different groups from those obtained from Canada and Europe. Furthermore, Chinese isolates from seven provinces, except those from Jiangsu, could be placed within the same cluster, based on geographical location. Nei’s gene diversity ranged from 0.25 in Jiangsu to 0.11 in Sichuan, and Shannon’s information index from 0.16 to 0.37. Genetic identity ranged from 0.784 to 0.834 when isolates from China were compared with those from Canada and the UK, respectively. Analysis of gene flow among populations (Nm) indicated that almost no gene exchange has occurred between L. biglobosa in China and the populations of the fungus from the UK or Canada. © 2014, Springer Science+Business Media Dordrecht.

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