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Pecina-Quintero V.,INIFAP Campo Experimental Bajio | Anaya-Lopez J.L.,INIFAP Campo Experimental Bajio | Nunez-Colin C.A.,INIFAP Campo Experimental Bajio | Zamarripa-Colmenero A.,INIFAP Campo Experimental Rosario Izapa | And 3 more authors.
Industrial Crops and Products | Year: 2013

Due to the chemical and physical properties of castor oil (Ricinus communis) that make it a valuable raw material for numerous industrial applications, including the production of biofuel, interest to develop more and better varieties has been increased. However, despite this interest, México does not currently have any varieties of enhanced castor for which yield and adaptation have been optimized to justify sizeable production. Although some varieties have been introduced, there are problems with adaptation. Given the need to develop castor bean varieties with outstanding agronomic characteristics and good adaptation, a collection of local R. communis seeds has been initiated. However, there has been wide phenotypic variation among collections. The objective of this study was to assess the diversity and genetic relationships among accessions of R. communis from the state of Chiapas, México using AFLP (amplified fragment length polymorphism) and SSR (simple sequence repeat) markers. In this research 53% of the AFLP markers were polymorphic; additionally, we detected high levels of genetic diversity (71%). The combinations E-AAC. +. M-CAG and E-ACG. +. M-CAG presented a PIC value of 0.415 and 0.425 and an RP of 41.98 and 46.11, respectively, indicating that these combinations are useful in studies of genetic characterization. Additionally, 100% of the SSR primers were polymorphic, with an average of 5.5 alleles per locus. The SSR primer Rco23 generated a PIC value of 0.812, an SI of 1.919 and four uncommon alleles (0.05, 0.06, 0.07, 0.07), indicating that this SSR primer was particularly informative for this study. A dendrogram was generated with the markers obtained from these studies, allowing for the identification of two main groups of accessions. Collectively, these studies will allow for the development of at least two different populations through the recombination of genotypes by genetic similarity and will provide a foundation for the breeding program. © 2012 Elsevier B.V.

Pecina-Quintero V.,INIFAP Campo Experimental Bajio | Anaya-Lopez J.L.,INIFAP Campo Experimental Bajio | Zamarripa-Colmenero A.,INIFAP Campo Experimental Rosario Izapa | Nunez-Colin C.A.,INIFAP Campo Experimental Bajio | And 3 more authors.
Biomass and Bioenergy | Year: 2014

The understanding of the genetic diversity and structure of populations of Jatropha curcas in their postulated centre of origin will permit to identify genetic material useful for future improvement of the species. Although it is estimated that Mexico is the likely centre of origin and domestication of J. curcas, so far the "true" centre of origin still has to be found. A representative set of 175 accessions of J. curcas from nine central and southeastern Mexican states (Chiapas, Veracruz, Oaxaca, Michoacán, Morelos, Yucatán, Guerrero, Hidalgo and Puebla), including toxic, non-toxic, and contrasting protein and oil content genotypes was used for diversity analysis by AFLP markers. The results indicate that Mexico has a high genetic diversity of J. curcas; molecular analysis suggests population structuring in the different states where this species is spread. The germplasm from Chiapas, where contrasting protein and oil content genotypes were detected, showed the highest genetic diversity and clearly varies from the accessions from the other states. This is probably the most comprehensive study of diversity of germplasm of J. curcas from Mexico, and together with previous reports on the genetic diversity, biochemistry, morphology and germplasm agronomics of J. curcas from Chiapas, suggest the likelihood that this area is the centre of origin for this species and that domestication took place in the states bordering the Gulf of Mexico, such as Veracruz, Hidalgo, Puebla and Yucatán, where genotypes with low or no phorbol ester content exist. © 2013 Elsevier Ltd.

Pecina-Quintero V.,INIFAP Campo Experimental Bajio | Anaya-Lopez J.L.,INIFAP Campo Experimental Bajio | Colmenero A.Z.,INIFAP Campo Experimental Rosario Izapa | Garcia N.M.,INIFAP Campo Experimental Rio Bravo | And 5 more authors.
Biomass and Bioenergy | Year: 2011

The aim of this study was to evaluate the genetic diversity of Jatropha curcas in Chiapas, México using AFLP markers. The AFLP technique allowed us to estimate the genetic relationships in the germplasm of J. curcas and to detect a high level of polymorphism. Analysis of the frequency and distribution of polymorphic fragments allowed us to detect the highest number of rare fragments in one single accession (e.g., accession number 1 from Tuxtla Chico). Besides being an accession that exhibits traits of agronomic importance (such as the presence of 100% pistillated flowers), this diversity of rare fragments may allow for development of markers for identification of this accession and/or development of markers linked to this trait. In addition, very divergent collections have been detected from regions where high average oil content and other characters associated with productivity have been found. Moreover, the analysis of genetic relationships, Analysis of Molecular Variance (AMOVA) and the diversity index (DI = 60%) confirm a broad gene pool in the J. curcas germplasm from Chiapas, México. The high diversity found within the accessions showed non-random mating between groups and/or regions of accessions of J. curcas, which complicates the selection of representative collections. © 2011 Elsevier Ltd.

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