Yu S.-L.,Inha Research Institute for Medical science |
An Y.J.,Seoul National University |
Kang M.-S.,Center for Advanced Medical Education by 21 Project |
Kim H.-Y.,Center for Advanced Medical Education by 21 Project |
And 6 more authors.
Journal of Proteome Research | Year: 2013
Alterations in metabolic pathways are gaining attention as important environmental factors affecting life span, but the determination of specific metabolic pathways and enzymes involved in life span remains largely unexplored. By applying an NMR-based metabolomics approach to a calorie-restricted yeast (Saccharomyces cerevisiae) model, we found that alanine level is inversely correlated with yeast chronological life span. The involvement of the alanine-metabolizing pathway in the life span was tested using a deletion mutant of ALT1, the gene for a key alanine-metabolizing enzyme. The mutant exhibited increased endogenous alanine level and much shorter life span, demonstrating the importance of ALT1 and alanine metabolic pathways in the life span. ALT1's effect on life span was independent of the TOR pathway, as revealed by a tor1 deletion mutant. Further mechanistic studies showed that alt1 deletion suppresses cytochrome c oxidase subunit 2 expression, ultimately generating reactive oxygen species. Overall, ALT1 seems critical in determining yeast life span, and our approach should be useful for the mechanistic studies of life span determinations. © 2013 American Chemical Society.
PubMed | Inha Research Institute for Medical Science, Inha University and Sookmyung Womens University
Type: | Journal: Neuroscience letters | Year: 2016
Bone marrow-derived mesenchymal stem cells (MSCs) are used in stroke treatment despite the poor understanding of its mode of action. The immune suppressive and anti-inflammatory properties of MSCs possibly play important roles in regulating neuroinflammation after stroke. We investigated whether MSCs reduce the inflammatory complement component 3 (C3) levels, thus, providing neuroprotection during stroke. Mice were subjected to transient focal cerebral ischemia (tFCI), after which MSCs were intravenously injected. The infarct volume of the brain was reduced in MSC-injected tFCI mice, and C3 expression was significantly reduced in both the brain and the blood. Additionally, the profiles of other inflammatory mediators demonstrated neuroprotective changes in the MSCs-treated group. In order to analyze the effect of MSCs on neurons during cerebral ischemia, primary cortical neurons were co-cultured with MSCs under oxygen-glucose deprivation (OGD). Primary neurons co-cultured with MSCs exhibited reduced levels of C3 expression and increased protection against OGD, indicating that treatment with MSCs reduces excessive C3 expression and rescues ischemia-induced neuronal damage. Our finding suggests that reduction of C3 expression by MSCs can help to ameliorate ischemic brain damage, offering a new neuroprotective strategy in stroke therapy.
Yi T.,Inha University |
Yi T.,Inha Research Institute for Medical science |
Yi T.,HomeoTherapy Co. |
Lee D.-S.,Inha University |
And 6 more authors.
Gene | Year: 2012
Emerging evidence of the potent immunosuppressive activity of mesenchymal stem cells (MSCs) by modulation of both innate and adaptive immune responses enables MSCs to be developed as a promising therapeutic modality for immune-related or inflammatory diseases. However, it is not clearly understood how MSCs exert their immunosuppressive effects on immune cells under inflammatory conditions. Using human bone marrow (BM)-derived clonal MSCs (hcMSCs), we obtained and analyzed a differentially expressed gene profile when stimulated with the inflammatory cytokines interferon-γ (IFN-γ) and tumor necrosis factor-α (TNF-α) to find novel candidate factors responsible for MSC immunomodulation. Microarray analysis showed that 5650 genes were upregulated and 5862 genes were downregulated with the cutoff of 2-fold expression change. Among these, the ICOSLG and STAT2 genes were drastically upregulated 173-fold and 154-fold, respectively. Reverse transcription-polymerase chain reaction analysis confirmed the microarray data. To evaluate whether their increased expression is related to MSC-mediated immunosuppression, siRNA-induced ICOSLG- or STAT2-knockdown hcMSCs were assessed for their T cell suppressive activity. We demonstrated that STAT2 but not ICOSLG is functionally involved in the immunosuppressive activity of hcMSCs as a novel regulator under inflammatory conditions. Gene ontology and pathway analyses further support the immunomodulatory function of hcMSCs when inflammatory stimulation was provided. Taken together, this study provides an informative genome-wide gene expression profile and molecular evidence for understanding the mechanisms underlying the modulation of immune cells by human BM-derived MSCs under inflammatory conditions. © 2012 Elsevier B.V.
Hwang S.H.,Kettering General Hospital |
Hwang S.H.,Inha Research Institute for Medical Science |
Hwang K.,Inha University
Journal of Craniofacial Surgery | Year: 2015
The aim of this paper is to see how the plastic surgeons are depicted in some recently made parodies of altar portraits of Buddha. Three of Kim's traditional paintings depicting a plastic surgeon were collected and 3 types of altar portraits of Buddha were also collected. The Water-Moon Avalokiteshvara sits on a rocky outcropping above the waves. At the lower right, is the boy pilgrim Sudhana. In the "Plastic Surgeon as a Bodhisattva," the plastic surgeon is wreathed in gold necklaces and seated on stones as if he were a wise man or perhaps a divine being, only it is his services that help allow for transformation. Below him, there is a female who yearns for man-made beauty. In Emma's court, there is a "Mirror of Perfect Clarity" that reflects unfailingly, the past misconduct and sins of the dead. In "Judgment of the Obese", the plastic surgeon looks down on his patients from above and makes severe judgments about their looks. The women are holding their hands out desperately, standing haggard in front of the mirror, pleading to the doctor. The Great Master of Seon Buddhism holds a large fly-whisk. In the "Portrait of a Plastic Surgeon", a surgeon is sitting in a chair holding a huge surgical knife as if the patriarch holds a monk's stick. Like the patients at our clinic and the sole of the dead at the Emma's court, we plastic surgeons should have a "Mirror" to reflect our practices and ask ourselves whether we are "good" doctors or not. Copyright © 2015 by Mutaz B. Habal, MD.
Hwang S.W.,Peninsula Medical School |
Hwang S.W.,Inha Research Institute for Medical science |
Nam Y.S.,Catholic University of Korea |
Hwang K.,Inha University |
Han S.H.,Catholic University of Korea
Journal of Anatomy | Year: 2012
The aim of this study is to elucidate the thickness and tension of the gluteal aponeurosis (GA) as related to subfascial gluteal augmentation. Twenty buttocks from 10 Korean fresh cadavers (age range: 69-92years, five men and five women) were dissected. Five radial lines were made from the greater trochanter (GT) to the highest point of origin of the gluteus maximus muscle (GM), the posterior inferior iliac spine (PSIS), the piriformis line (P), the coccyx (Co) and the ischial tuberosity (IT). The upper four lines were intersected by three curvilinear lines that divided them by a quarter, half and three-quarters ratios, and the lowest line was divided by a third ratio and a two-thirds ratio. At the 14 intersecting points, the force needed to break the 6mm width of the GA was measured. The thickness of the GA was also measured with a digital caliper. The GA was widest at the GT-Co line (161.7±15.8mm), and it was narrowest at the GT-IT line (106.5±21.2mm). At most of the points (12 among the 14 points), the breaking strength of the GA was greater than 20Newtons (N). The breaking strength of the GA did not vary significantly according to the locations (P=0.568, anova). The breaking strength of the males (22.8±6.6N) was significantly greater than that of the females (20.3±7.5N, P=0.003, t-test). The thickness of the GA varied according to the locations (0.4±0.2mm to 0.7±0.3mm). The thickness of the GA of the upper part (GT-GM line: 0.64±0.24mm; GT-PSIS line: 0.66±0.23mm; GT-P line: 0.66±0.24mm) was significantly greater (P=0.040, 0.017, 0.018, respectively) than that of the lower part (GT-IT line: 0.49±0.18mm). The GA of the males (0.70±0.23mm) was significantly thicker than that of the females (0.53±0.21mm, P<0.001, t-test). We conclude that the GA is capable of holding gluteal implants in the proper position, as the average force to break up the 6mm width of the GA in females was greater than 20N. © 2012 The Authors. Journal of Anatomy © 2012 Anatomical Society.
Hwang K.,Inha University |
Park J.Y.,Inha University |
Hwang S.W.,Peninsula Medical School |
Hwang S.W.,Inha Research Institute for Medical science
Archives of Plastic Surgery | Year: 2015
The aim of this study is to consider breast imagery in art as depicted through western painting. Twenty western art paintings were collated. Most of the sample paintings were created from the mid-nineteenth century to the late twentieth century and some are from the Renaissance period. Ten anthropometric items were used to measure 15 distances between two landmarks and 3 angles between three points. The distance from the nipple to the sternal notch and to the midclavicular point was the same and they were 0.46 of the distance from the sternal notch to the umbilicus. The shape of the projection of the breast was almost an isosceles triangle and the altitude of the triangle was at a proportion of 0.45 of the bottom length and 0.16 of the distance from the sternal notch to the umbilicus. The distance between the lateral ends of the breasts was 2.14 times the facial width and the distance between nipples was 1.36 times the facial width. Proportions from works of art are more ideal and attractive than clinically measured proportions. The desirable ratios measured from historical paintings might be useful in planning breast surgeries. © 2015, The Korean Society of Plastic and Reconstructive Surgeons.
Park C.-S.,Inha Research Institute for Medical science |
Jang T.Y.,Inha University |
Heo M.-J.,Inha University |
Jung A.-Y.,Inha University |
Kim Y.H.,Inha University
American Journal of Rhinology and Allergy | Year: 2016
Objective: We aimed to find novel genes that are significantly induced in allergic mice and that are significantly downregulated with anti-interleukin (IL) 33 treatment. Methods: Thirty-six mice were allocated into each of group A (intraperitoneal [i.p.]) sensitized and intranasally challenged to saline solution), group B (sensitized and challenged to ovalbumin), group C (sensitized and challenged with ovalbumin, and null treatment with i.p. saline solution), and group D (sensitized and challenged with ovalbumin, and treatment with anti-IL-33 i.p. injection). We counted the number of nose-scratching in 10 minutes, serum ovalbumin-specific immunoglobulin E (IgE), and titers of cytokines (IL-1, IL-4, IL-5, IL-10, IL-13) in bronchoalveolar lavage fluid. By using one whole lung from each mouse, we performed microarray analysis and real-time polymerase chain reaction. Results: group D showed a significantly reduced nose-scratching events and lower serum ovalbumin-specific IgE compared with groups B and C. All the cytokines in the bronchoalveolar lavage fluid were significantly decreased after anti-IL-33 treatment. Microarray analysis revealed that group B (immunoglobulin free light chain [IgFLC], 89.1 times; nitric oxide synthase [NOS] 2, 11.5 times) and group C (IgFLC, 141.6 times; NOS2, 11.7 times) had significantly increased expression of IgFLC and NOS2 genes compared with group A. After anti-IL-33 treatment, group D showed significantly decreased expression of both IgFLC (49.3 times) and NOS2 (6.5 times). In real-time polymerase chain reaction, groups B and C had significantly increased expression of these genes (IgFLC, 10.4 times and 29 times, respectively; NOS2, 3.8 times and 4.5 times, respectively). After treatment, group D showed significantly decreased expression of IgFLC (5.0 times) and NOS2 (2.5 times). Conclusion: The antiallergic effect of anti-IL-33 can be explained by suppression of IgFLC and NOS2 in a murine model of allergic rhinitis. Copyright © 2016, OceanSide Publications, Inc., U.S.A.
PubMed | Inha Research Institute for Medical science and Inha University
Type: Journal Article | Journal: Archives of plastic surgery | Year: 2015
The aim of this study is to consider breast imagery in art as depicted through western painting. Twenty western art paintings were collated. Most of the sample paintings were created from the mid-nineteenth century to the late twentieth century and some are from the Renaissance period. Ten anthropometric items were used to measure 15 distances between two landmarks and 3 angles between three points. The distance from the nipple to the sternal notch and to the midclavicular point was the same and they were 0.46 of the distance from the sternal notch to the umbilicus. The shape of the projection of the breast was almost an isosceles triangle and the altitude of the triangle was at a proportion of 0.45 of the bottom length and 0.16 of the distance from the sternal notch to the umbilicus. The distance between the lateral ends of the breasts was 2.14 times the facial width and the distance between nipples was 1.36 times the facial width. Proportions from works of art are more ideal and attractive than clinically measured proportions. The desirable ratios measured from historical paintings might be useful in planning breast surgeries.
PubMed | Inha Research Institute for Medical Science and Inha University
Type: Journal Article | Journal: Korean journal of medical education | Year: 2016
The aim of this study is to see what medical students think about the role of spouse of a devoted medical doctor through the book reports of The Painted Veil (1925).The 53 medical students were asked to read Maughams The Painted Veil and to have a discussion. In their book reports, following questions were asked to be included: What it is like to be married a devoted medical doctor? Do you think that patients realize, value, and respect the importance of doctors work? In the outbreak of highly infectious and fatal disease, can you carry out a heroic fight to control it?Among the 53 respondents, seven students (13%) answered that they would be happy if they marry a devoted doctor and scientist and 34 (64%) unhappy. The remaining 12 (23%) could not make a decision. The six students (11%) answered that doctor is valued and respected by patients while 46 (87%) answered doctor is neither valued nor respected. The remaining one (2%) could not decide. The 20 students (38%) answered that they would fight for the infectious disease and the remaining 30 (57%) answered that they would not. The remaining three (5%) could not determine their mind.The Painted Veil induced a virtue of life of balance and harmony and attitude of doctor who give superiority to responsibility and duty over prestige and wealth from the medical students. It could be a good teaching material for medical humanity.
PubMed | Inha Research Institute for Medical science
Type: | Journal: Neuroscience letters | Year: 2014
Ethyl pyruvate (EP) has been shown to have anti-inflammatory effects and confer protective effects in various pathological conditions. For example, EP inhibits secretion of high mobility group box 1 (HMGB1), which is known to be released from activated or dying cells and aggravate inflammatory pathways. In the present study, we investigated whether EP reduces HMGB1 phosphorylation and release in ischemic brain and in cultured microglia. In the postischemic brains (60 min middle cerebral artery occlusion (MCAO)), HMGB1 was released extracellularly, generating dual peaks in cerebrospinal fluid (CSF) around 1 and 7 days after ischemic insult, which were probably generated from damaged neurons and activated inflammatory cells, respectively. We showed that treatment with EP 30 min post-MCAO (5 mg/kg, i.v.), which has been shown to confer a robust neuroprotective effect in the postischemic brain, reduced both peaks. In addition, delayed EP treatment from 4 days post-MCAO reduced HMGB1 accumulation in CSF at 7 day post-MCAO in the absence of accompanying amelioration of ischemic brain damage, indicating that the suppression of HMGB1 release is a direct effect. We also found that EP markedly suppressed the LPS-induced nuclear translocations of protein kinase C alpha and calcium/calmodulin-dependent protein kinase IV, HMGB1 phosphorylation, and subsequent secretion of HMGB1 induced by LPS in BV2 cells and EP-mediated above-mentioned effects were also independent of cell death or survival. These results indicate that EP inhibits HMGB1 phosphorylation and release in activated microglia, which might be responsible for EP-mediated suppression of HMGB1 release in the postischemic brain.