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Abeysekera W.K.S.M.,Industrial Technology Institute ITI 363 | Premakumara G.A.S.,Industrial Technology Institute ITI 363 | Dar A.,University of Karachi | Choudhary M.I.,University of Karachi | And 5 more authors.
Journal of Food Biochemistry | Year: 2015

Extracts, fractions and gastrointestinal-resistant protein hydrolysates (GRPH) from rice bran (RB) of four Sri Lankan traditional varieties were studied for growth inhibition (GI) and cytotoxicity against human lung cancer (NCI-H460), cervical cancer (HeLa) cell lines and effect on glutathione S-transferase (GST) in vitro. RB extracts showed significantly high (P<0.05) GI (GI50μg/mL: HeLa: 19.24±3.47 to 112.58±0.05; NCI-H460: 54.63±4.89 to 170.31±4.71) and cytotoxicity (LC50μg/mL: HeLa: 240.12±9.23 to 476.22±0.05; NCI-H460: 255.36±1.81 to 412.07±17.73) compared with GRPH. Highest GI (GI50: 30.63±9.19 to 44.40±5.34μg/mL) and cytotoxicity (LC50: 141.31±14.47 to 169.51±2.04μg/mL) was evident in nonpolar fractions (dichloromethane: Goda Heeneti and Sudu Heeneti; hexane: Dik Wee and Sudu Heeneti). No cytotoxicity was evident to normal cells. All RB extracts had high GST inhibition. Properties observed indicate its usefulness in managing lung and cervical cancers. © 2015 Wiley Periodicals, Inc. Source


Hewajulige I.G.N.,Industrial Technology Institute ITI 363 | Wilson Wijeratnam R.S.,Industrial Technology Institute ITI 363 | Perera M.G.D.S.,Industrial Technology Institute ITI 363 | Fernando S.A.,Industrial Technology Institute ITI 363
Acta Horticulturae | Year: 2015

Commercial storage of papaya and mango is restricted by postharvest diseases caused by fungi. The use of bio-waxes on storage life extension and quality retention of these commodities was investigated. Three different bio-waxes (chitosan, ITI wax 1 and ITI wax 2) formulated from locally available ingredients were used in this study. Papaya and mango harvested at commercial maturity were dipped in wax formulations and stored at low temperature at 13°C and 80% relative humidity for 7, 14 and 21 days. Since, chitosan is reported as an antifungal compound, an in vitro antifungal assay was also conducted for disease causing fungi, Colletotrichum spp. and Botryodiplodia spp. isolated from fruit prior to in vivo assay. In vitro studies on chitosan revealed significant inhibition on radial mycelial growth of the disease causing organisms isolated from these commodities at treatments of 1% chitosan and above. Significant changes were observed in all bio-wax coated fruits compared with non-treated controls with respect to peel colour, firmness, internal CO2 concentration and respiration rate while other organoleptic characteristics of fruit were not affected. Both papaya and mango coated with 1% chitosan and ITI wax 1 showed improved keeping quality with high marketability throughout the storage period of 14 days at 13±1°C plus 2 days at 28±2°C. Mangoes coated with the ITI wax 1 formulation showed storage life extension of 17 days at the same temperature. Significant increase in the activity of plant defense enzymes, chitinase and b, 1-3, glucanase were observed in the papaya peel samples of chitosan coated fruits compared to controls. The results of this study confirmed that the storage life of these tropical fruits could be extended using bio-waxes without detrimental effects on external and internal quality of fruit. Source

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