Industrial Technology Institute ITI

Colombo, Sri Lanka

Industrial Technology Institute ITI

Colombo, Sri Lanka
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Gunathilake K.G.T.,University of Sri Jayewardenepura | Herath T.,Industrial Technology Institute ITI | Wansapala J.,University of Sri Jayewardenepura
Potravinarstvo | Year: 2016

Grain legumes are widely used as high-protein contained crops that play a secondary role to cereal or root crops. In Sri Lanka various legume species are cultivated and often utilised in the whole grain boiled form. The objective of present study was to analyse and compare locally grown legumes varieties; Mung bean (MI 5, MI 6), Cowpea (Bombay, Waruni, Dhawal, MICP1, ANKCP1) and soybean (pb1, MISB1) for their morphological characteristics, proximate and mineral composition (Fe, Ca, Zn, K, P). Seed shape, seed coat texture and colour, seed size and 100 seed weight (g) were observed morphological characteristics in present study. Most of the characteristics of mung bean and soybean were similar within their species whereas characteristics of cowpea varieties largely differed. Values of 100 seed weight among the varieties of mung bean, soybean and cowpea were ranged from 5.8 - 6.5 g, 13.5 - 14.1 g and 13.4 - 17.2 g, respectively. The moisture content of all legume seeds ranged from 6.81% to 11.99%. Results were shown that the protein content significantly higher in soybean (36.56 - 39.70%) followed by mung bean (26.56 - 25.99%) and cowpea (25.22 - 22.84%) respectively. Range of total carbohydrate, crude fat, crude fibre and total ash contents of nine legume varieties varied from 15.29 - 62.97%, 1.25 - 22.02%, 3.04 - 7.93% and 3.43 - 6.35 respectively. potassium (K), phosphorus (P), calcium (Ca), iron (Fe) and zinc (Zn) ranged from 1000 - 1900, 360 - 669, 15.0 - 192.3, 2.26 - 11.6 and 1.67 - 4.26 mg.100g-1 respectively in all the species of studied legume varieties. The wide variation in the chemical and physical properties of observed nine legume varieties, suggesting possible applications for various end-use products. © 2016 Potravinarstvo. All rights reserved.

Herath H.M.T.,Industrial Technology Institute ITI | Rajapakse D.,Industrial Technology Institute ITI | Wimalasena S.,University of Kelaniya | Weerasooriya M.K.B.,University of Kelaniya
International Journal of Food Science and Technology | Year: 2011

Thirty-eight rice varieties, grown in Low Country Wet Zone, during Yala (2006) and Maha (2006/2007) seasons were screened for stable high iron rice varieties and were statistically analysed. Iron contents ranged 1.89-3.73mg 100g-1 and varied significantly (P<0.05) with variety and season. Selected fifteen high iron rice varieties, at degree of polishing 8-10% were analysed for iron, zinc and phytate. High iron contents in endosperm were observed in Suduru Samba (0.47mg 100g-1), Basmati 370 (0.37mg 100g-1), Kalu Heenati (0.42mg 100g-1), Rathu Heenati (0.44mg 100g-1) and Sudu Heenati (0.37mg 100g-1). Phytate contents of polished rice varieties ranged from 200-300mg 100g-1. Large reduction in iron content (84.5-93.6%) was observed in polished rice while the reduction in phytate content (18.9-40.8%) was low. Percentage dialyzability of iron in selected endosperm high iron rice varieties ranged from 1.73 to 8.71. Dialysability of iron in cooked polished rice did not show a relation to the phytate content in raw rice. © 2011 The Authors. International Journal of Food Science and Technology © 2011 Institute of Food Science and Technology.

Perera H.D.S.M.,Industrial Technology Institute ITI | Samarasekera J.K.R.R.,Industrial Technology Institute ITI | Handunnetti S.M.,University of Colombo | Weerasena O.V.D.S.J.,University of Colombo
Industrial Crops and Products | Year: 2016

The search for novel sources of new anti-inflammatory agents has gained an increasing demand with the rising number of indications of inflammatory mediated diseases. Traditionally, medicinal plant extracts have been used to treat number of diseases mediated by inflammation and still remain as potent sources of new anti-inflammatory agents and antioxidants. Therefore, the present study was undertaken to evaluate the in vitro anti-inflammatory and anti-oxidant properties of ten Sri Lankan medicinal plant extracts, traditionally used to treat diseases associated with inflammation. Arachidonate-5-lipoxygenase (A5-LOX), hyaluronidase, xanthine oxidase enzyme inhibitory assays and nitric oxide (NO) production inhibitory assay in lipopolysaccharide activated RAW 264.7 macrophages were used to evaluate in vitro anti-inflammatory activity. Antioxidant activity, total polyphenolic and total flavonoid contents were determined using six standard in vitro assays. The extract of Murraya koenigii L.exhibited the highest anti-A5-LOX activity (IC50 = 7.83 ± 0.42 μg/mL), while that of Symplocos cochinchinesis Lour. showed high anti-hyaluronidase activity (69.35%) along with a high oxygen radical absorbance capacity (ORAC) (1539 ± 31 mg TE/g). The extract of Calophyllum innophyllum L. also exhibited high anti-hyaluronidase activity (68.45% at 500 μg/mL), xanthine oxidase inhibitory activity (60.63% at 250 μg/mL), NO production inhibitory activity (35.00% at 500 μg/mL) along with a marked 2,2-diphenyl-2-picryl-hydrazyl (DPPH) free radical scavenging activity (IC50 = 4.42 ± 0.03 μg/mL) and ferric reducing antioxidant power (FRAP) (8231 ± 5 mg TE/g). Ferrous ion chelating (FIC) activity of plant extracts was found to be low in comparison with the reference standard EDTA-Na2. Higher total polyphenol content (TPC) and total flavonoid content (TFC) were recorded for the extracts of Cyanometra cauliflora L. and Murraya koenigii L. respectively. All bio-activities were found to be significant at p < 0.05. In the correlations analysis, a high positive correlation was observed between anti-A5-LOX and DPPH free radical scavenging activities. Moderate, positive correlations were observed among NO production inhibitory, xanthine oxidase inhibitory activities, FRAP and ORAC of plant extracts. This is the first report demonstrating the anti-A5-LOX, anti-hyaluronidase, xanthine oxidase and nitric oxide production inhibitory activities of ethanol extracts of the tested Sri Lankan medicinal plant extracts. The findings of the study support the traditional uses of these plant extracts against inflammatory mediated diseases. Moreover, the extracts, having promising in vitro anti-inflammatory and antioxidant properties could be effectively used for pharmaceutical, neutraceutical as well as for cosmaceutical applications. © 2016

Abeysekera W.K.S.M.,Industrial Technology Institute ITI | Premakumara G.A.S.,Industrial Technology Institute ITI | Dar A.,University of Karachi | Choudhary M.I.,University of Karachi | And 5 more authors.
Journal of Food Biochemistry | Year: 2015

Extracts, fractions and gastrointestinal-resistant protein hydrolysates (GRPH) from rice bran (RB) of four Sri Lankan traditional varieties were studied for growth inhibition (GI) and cytotoxicity against human lung cancer (NCI-H460), cervical cancer (HeLa) cell lines and effect on glutathione S-transferase (GST) in vitro. RB extracts showed significantly high (P<0.05) GI (GI50μg/mL: HeLa: 19.24±3.47 to 112.58±0.05; NCI-H460: 54.63±4.89 to 170.31±4.71) and cytotoxicity (LC50μg/mL: HeLa: 240.12±9.23 to 476.22±0.05; NCI-H460: 255.36±1.81 to 412.07±17.73) compared with GRPH. Highest GI (GI50: 30.63±9.19 to 44.40±5.34μg/mL) and cytotoxicity (LC50: 141.31±14.47 to 169.51±2.04μg/mL) was evident in nonpolar fractions (dichloromethane: Goda Heeneti and Sudu Heeneti; hexane: Dik Wee and Sudu Heeneti). No cytotoxicity was evident to normal cells. All RB extracts had high GST inhibition. Properties observed indicate its usefulness in managing lung and cervical cancers. Practical Applications: Lung and cervical cancers are two of the most commonly diagnosed cancers in men and women, respectively, and leading causes of mortality. Although numerous potent chemotherapeutic drugs are available for management, most of these drugs are very expensive and are associated with numerous side effects. Further, anticancer drugs containing electrophilic centers can easily form a conjugate with glutathione, which is facilitated by glutathione S-transferase (GST) and excreted from the body resulting in lower efficacy. Therefore, GST inhibitors are used as adjuvants during cancer chemotherapy. The selected rice varieties in this study claimed to possess health-enhancing properties according to Sri Lankan ethnomedicine. The findings of this study clearly showed that bran extracts, fractions and gastrointestinal-resistant protein hydrolysates of selected rice varieties had growth inhibition and cytotoxicity against both lung and cervical cancers. Further, all of these selected varieties had high GST inhibition. Therefore, selected rice brans may be useful in the management of lung and cervical cancers. © 2015 Wiley Periodicals, Inc.

Premakumara G.A.S.,Industrial Technology Institute ITI | Abeysekera W.K.S.M.,Industrial Technology Institute ITI | Ratnasooriya W.D.,University of Colombo | Chandrasekharan N.V.,University of Colombo | Bentota A.P.,Rice Research and Development Center
Journal of Cereal Science | Year: 2013

Brans of 23 traditional and 12 improved (both red and white) rice varieties in Sri Lanka were screened for anti-amylase and anti-glycation activities invitro. Varieties which showed the highest inhibitory activities at screening were further investigated for anti-glucosidase and glycation reversing as anti-diabetic properties. The same varieties were studied for selected antioxidant properties. Significantly high anti-amylase and anti-glycation activities were observed for bran extracts of red varieties compared to white varieties at screening. Traditional red rice varieties, Masuran, Sudu Heeneti, Dik Wee and Goda Heeneti, exhibited significant and dose dependent anti-amylase, anti-glycation and glycation reversing activities. These varieties also showed marked antioxidant properties. It is concluded that brans of Sri Lankan traditional red rice varieties Masuran, Sudu Heeneti, Dik Wee and Goda Heeneti may be potential food supplements for diabetes. © 2013 Elsevier Ltd.

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