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Abeysekera W.K.S.M.,Industrial Technology Institute ITI | Premakumara G.A.S.,Industrial Technology Institute ITI | Dar A.,University of Karachi | Choudhary M.I.,University of Karachi | And 5 more authors.
Journal of Food Biochemistry | Year: 2015

Extracts, fractions and gastrointestinal-resistant protein hydrolysates (GRPH) from rice bran (RB) of four Sri Lankan traditional varieties were studied for growth inhibition (GI) and cytotoxicity against human lung cancer (NCI-H460), cervical cancer (HeLa) cell lines and effect on glutathione S-transferase (GST) in vitro. RB extracts showed significantly high (P<0.05) GI (GI50μg/mL: HeLa: 19.24±3.47 to 112.58±0.05; NCI-H460: 54.63±4.89 to 170.31±4.71) and cytotoxicity (LC50μg/mL: HeLa: 240.12±9.23 to 476.22±0.05; NCI-H460: 255.36±1.81 to 412.07±17.73) compared with GRPH. Highest GI (GI50: 30.63±9.19 to 44.40±5.34μg/mL) and cytotoxicity (LC50: 141.31±14.47 to 169.51±2.04μg/mL) was evident in nonpolar fractions (dichloromethane: Goda Heeneti and Sudu Heeneti; hexane: Dik Wee and Sudu Heeneti). No cytotoxicity was evident to normal cells. All RB extracts had high GST inhibition. Properties observed indicate its usefulness in managing lung and cervical cancers. Practical Applications: Lung and cervical cancers are two of the most commonly diagnosed cancers in men and women, respectively, and leading causes of mortality. Although numerous potent chemotherapeutic drugs are available for management, most of these drugs are very expensive and are associated with numerous side effects. Further, anticancer drugs containing electrophilic centers can easily form a conjugate with glutathione, which is facilitated by glutathione S-transferase (GST) and excreted from the body resulting in lower efficacy. Therefore, GST inhibitors are used as adjuvants during cancer chemotherapy. The selected rice varieties in this study claimed to possess health-enhancing properties according to Sri Lankan ethnomedicine. The findings of this study clearly showed that bran extracts, fractions and gastrointestinal-resistant protein hydrolysates of selected rice varieties had growth inhibition and cytotoxicity against both lung and cervical cancers. Further, all of these selected varieties had high GST inhibition. Therefore, selected rice brans may be useful in the management of lung and cervical cancers. © 2015 Wiley Periodicals, Inc.


Premakumara G.A.S.,Industrial Technology Institute ITI | Abeysekera W.K.S.M.,Industrial Technology Institute ITI | Ratnasooriya W.D.,University of Colombo | Chandrasekharan N.V.,University of Colombo | Bentota A.P.,Rice Research and Development Center
Journal of Cereal Science | Year: 2013

Brans of 23 traditional and 12 improved (both red and white) rice varieties in Sri Lanka were screened for anti-amylase and anti-glycation activities invitro. Varieties which showed the highest inhibitory activities at screening were further investigated for anti-glucosidase and glycation reversing as anti-diabetic properties. The same varieties were studied for selected antioxidant properties. Significantly high anti-amylase and anti-glycation activities were observed for bran extracts of red varieties compared to white varieties at screening. Traditional red rice varieties, Masuran, Sudu Heeneti, Dik Wee and Goda Heeneti, exhibited significant and dose dependent anti-amylase, anti-glycation and glycation reversing activities. These varieties also showed marked antioxidant properties. It is concluded that brans of Sri Lankan traditional red rice varieties Masuran, Sudu Heeneti, Dik Wee and Goda Heeneti may be potential food supplements for diabetes. © 2013 Elsevier Ltd.


Herath H.M.T.,Industrial Technology Institute ITI | Rajapakse D.,Industrial Technology Institute ITI | Wimalasena S.,University of Kelaniya | Weerasooriya M.K.B.,University of Kelaniya
International Journal of Food Science and Technology | Year: 2011

Thirty-eight rice varieties, grown in Low Country Wet Zone, during Yala (2006) and Maha (2006/2007) seasons were screened for stable high iron rice varieties and were statistically analysed. Iron contents ranged 1.89-3.73mg 100g-1 and varied significantly (P<0.05) with variety and season. Selected fifteen high iron rice varieties, at degree of polishing 8-10% were analysed for iron, zinc and phytate. High iron contents in endosperm were observed in Suduru Samba (0.47mg 100g-1), Basmati 370 (0.37mg 100g-1), Kalu Heenati (0.42mg 100g-1), Rathu Heenati (0.44mg 100g-1) and Sudu Heenati (0.37mg 100g-1). Phytate contents of polished rice varieties ranged from 200-300mg 100g-1. Large reduction in iron content (84.5-93.6%) was observed in polished rice while the reduction in phytate content (18.9-40.8%) was low. Percentage dialyzability of iron in selected endosperm high iron rice varieties ranged from 1.73 to 8.71. Dialysability of iron in cooked polished rice did not show a relation to the phytate content in raw rice. © 2011 The Authors. International Journal of Food Science and Technology © 2011 Institute of Food Science and Technology.

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