Industrial Technology Institute ITI

Colombo, Sri Lanka

Industrial Technology Institute ITI

Colombo, Sri Lanka
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Gunathilake K.G.T.,University of Sri Jayewardenepura | Herath T.,Industrial Technology Institute ITI | Wansapala J.,University of Sri Jayewardenepura
Potravinarstvo | Year: 2016

Grain legumes are widely used as high-protein contained crops that play a secondary role to cereal or root crops. In Sri Lanka various legume species are cultivated and often utilised in the whole grain boiled form. The objective of present study was to analyse and compare locally grown legumes varieties; Mung bean (MI 5, MI 6), Cowpea (Bombay, Waruni, Dhawal, MICP1, ANKCP1) and soybean (pb1, MISB1) for their morphological characteristics, proximate and mineral composition (Fe, Ca, Zn, K, P). Seed shape, seed coat texture and colour, seed size and 100 seed weight (g) were observed morphological characteristics in present study. Most of the characteristics of mung bean and soybean were similar within their species whereas characteristics of cowpea varieties largely differed. Values of 100 seed weight among the varieties of mung bean, soybean and cowpea were ranged from 5.8 - 6.5 g, 13.5 - 14.1 g and 13.4 - 17.2 g, respectively. The moisture content of all legume seeds ranged from 6.81% to 11.99%. Results were shown that the protein content significantly higher in soybean (36.56 - 39.70%) followed by mung bean (26.56 - 25.99%) and cowpea (25.22 - 22.84%) respectively. Range of total carbohydrate, crude fat, crude fibre and total ash contents of nine legume varieties varied from 15.29 - 62.97%, 1.25 - 22.02%, 3.04 - 7.93% and 3.43 - 6.35 respectively. potassium (K), phosphorus (P), calcium (Ca), iron (Fe) and zinc (Zn) ranged from 1000 - 1900, 360 - 669, 15.0 - 192.3, 2.26 - 11.6 and 1.67 - 4.26 mg.100g-1 respectively in all the species of studied legume varieties. The wide variation in the chemical and physical properties of observed nine legume varieties, suggesting possible applications for various end-use products. © 2016 Potravinarstvo. All rights reserved.

Shanura Fernando I.P.,Jeju National University | Asanka Sanjeewa K.K.,Jeju National University | Samarakoon K.W.,Industrial Technology Institute ITI | Lee W.W.,Jeju National University | And 8 more authors.
Algae | Year: 2017

Polysaccharides of marine algae exhibit different structural characteristics and interesting biological functions. In this study, crude polysaccharides (CP) of eleven Sri Lankan marine algae obtained through hot water extraction and ethanol precipitation were investigated for DPPH, alkyl, and hydroxyl radical scavenging activities using electron spin resonance spectrometry and for intracellular reactive oxygen species scavenging activity in the Chang liver cell line. Characterization of CPs was done by Fourier transform infrared (FTIR) spectroscopy and by analysis of the monosaccharide composition. Time-dependent density functional theory quantum-chemical calculations at the RB3LYP/6-31G(d,p) level for constructed dimeric units of the corresponding polysaccharides were used to resolve the FTIR spectra. CPs from Chnoospora minima showed the highest DPPH and alkyl radical scavenging activities and higher intracellular reactive oxygen species scavenging effects for both AAPH and H2O2 induced ROS production in “Chang” cells. The major polysaccharide constituent in C. minima CP was identified as fucoidan and it displayed a higher sulfate content. The degree of sulfation of these polysaccharides suggests a positive correlation with the observed antioxidant properties. © 2017 The Korean Society of Phycology.

Ranaweera C.B.,Health Science University | Abeysekara W.P.,Industrial Technology Institute ITI | Pathirana R.,Health Science University | Ratnasooriya W.D.,Health Science University
Journal of Pharmaceutical Negative Results | Year: 2015

Objective: To assess the antihyaluronidase activity of methanolic leaf extract of Indigofera tinctoria L (I. tinctoria) (family: Fabaceae/Leguminosae) and stem bark extract of Stereospermum suaveolens DC (S. suaveolens) (family: Bignoniaceae) in vitro with a view to develop an antiaging skin formulation. Materials and Methods: The antihyaluronidase activity of different concentrations (0.19 mg/mL, 0.38 mg/mL, 0.75 mg/mL, 1.5 mg/mL, and 3.0 mg/mL) of methanolic leaf extract of I. tinctoria, methanolic stem bark extract of S. suaveolens, and reference drug epigallocatechin gallate (EGCG) of different concentrations (12.5 μg/mL, 25 μg/mL, 50 μg/mL, 100 μg/mL, and 200 μg/mL) were determined spectrophotometrically using hyaluronic acid (from rooster combs) and bovine testicular hyaluronidase. Results: There is no in vitro antihyaluronidase activity in the methanolic extracts of I. tinctoria leaves and S. suaveolens stem bark even at high concentrations. On the contrary, EGCG, the reference agent, showed marked concentration.dependent (r2 = 0.92) antihyluronidase activity [in terms of percentage inhibition: half maximal inhibitory concentration (IC50) 92.64 ± 0.64 μg/mL]. Conclusion: It is unlikely that skin antiaging effects of I. tinctoria leaves and S. suaveolens stem bark, as claimed in traditional and folk medicines in Sri Lanka, are mediated via antihyaluronidase activity.

Fernando I.P.S.,Jeju National University | Sanjeewa K.K.A.,Jeju National University | Samarakoon K.W.,Industrial Technology Institute ITI | Lee W.W.,Jeju National University | And 5 more authors.
International Journal of Biological Macromolecules | Year: 2017

Fucoidans are an interesting group of bioactive sulfated polysaccharides abundant in brown seaweeds. The current study highlights the enrichment and extraction of fucoidan from Chnoospora minima by means of enzyme-assistant extraction using Celluclast and evaluation of its anti-inflammatory potential through in vitro and in vivo studies. The purified C. minima fucoidan (F2,4) inhibited the nitrous oxide (NO) production (IC50 = 27.82 ± 0.88 μg/ml) and expression of PGE2 through the subsequent downregulation of iNOS and COX-2 expression in lipopolysaccharide (LPS) stimulated RAW 264.7 macrophages. F2,4 downregulated TNF-α, IL1-β, and IL-6 in RAW 264.7 macrophages in a dose-dependent manner and suppressed NO and ROS production in LPS stimulated zebrafish embryos while exerting a protective effect against the cell damage caused by LPS. Polysaccharide structural characterization was performed using FTIR, HPAE-PAD analysis of the monosaccharide content and NMR spectroscopy. Current findings confirm the potential anti-inflammatory activity of fucoidan purified from C. minima and elaborate its potential application as a functional ingredient in consumer products. © 2017 Elsevier B.V.

Abeysekera W.P.K.M.,Industrial Technology Institute ITI | Arachchige S.P.G.,Industrial Technology Institute ITI | Ratnasooriya W.D.,University of Colombo | Ratnasooriya W.D.,Health Science University
Evidence-based Complementary and Alternative Medicine | Year: 2017

Ethanol (95%) and dichloromethane: methanol (1: 1) bark extracts of authenticated Ceylon cinnamon were investigated for range of antilipidemic activities (ALA): HMG-CoA reductase, lipase, cholesterol esterase, and cholesterol micellization inhibitory activities and bile acids binding in vitro. Individual compounds in bark extracts were also evaluated. Bark extracts showed ALA in all the assays studied. The IC50 (μg/mL) values ranged within 153.07±8.38-277.13±32.18, 297.57±11.78-301.09±4.05, 30.61±0.79-34.05±0.41, and 231.96±9.22-478.89±9.27, respectively, for HMG-CoA reductase, lipase, cholesterol esterase, and cholesterol micellization inhibitory activities. The bile acids binding (3 mg/mL) for taurocholate, glycodeoxycholate, and chenodeoxycholate ranged within 19.74±0.31-20.22±0.31, 21.97±2.21-26.97±1.61, and 16.11±1.42-19.11±1.52%, respectively. The observed ALA were moderate compared to the reference drugs studied. Individual compounds in bark extracts ranged within 2.14±0.28-101.91±3.61 and 0.42±0.03-49.12±1.89 mg/g of extract. Cinnamaldehyde and gallic acid were the highest and the lowest among the tested compounds. The ethanol extract had highest quantity of individual compounds and ALA investigated. Properties observed indicate usefulness of Ceylon cinnamon bark in managing hyperlipidemia and obesity worldwide. Further, this study provides scientific evidence for the traditional claim that Ceylon cinnamon has antilipidemic activities. © 2017 Walimuni Prabhashini Kaushalya Mendis Abeysekera et al.

Herath H.M.T.,Industrial Technology Institute ITI | Rajapakse D.,Industrial Technology Institute ITI | Wimalasena S.,University of Kelaniya | Weerasooriya M.K.B.,University of Kelaniya
International Journal of Food Science and Technology | Year: 2011

Thirty-eight rice varieties, grown in Low Country Wet Zone, during Yala (2006) and Maha (2006/2007) seasons were screened for stable high iron rice varieties and were statistically analysed. Iron contents ranged 1.89-3.73mg 100g-1 and varied significantly (P<0.05) with variety and season. Selected fifteen high iron rice varieties, at degree of polishing 8-10% were analysed for iron, zinc and phytate. High iron contents in endosperm were observed in Suduru Samba (0.47mg 100g-1), Basmati 370 (0.37mg 100g-1), Kalu Heenati (0.42mg 100g-1), Rathu Heenati (0.44mg 100g-1) and Sudu Heenati (0.37mg 100g-1). Phytate contents of polished rice varieties ranged from 200-300mg 100g-1. Large reduction in iron content (84.5-93.6%) was observed in polished rice while the reduction in phytate content (18.9-40.8%) was low. Percentage dialyzability of iron in selected endosperm high iron rice varieties ranged from 1.73 to 8.71. Dialysability of iron in cooked polished rice did not show a relation to the phytate content in raw rice. © 2011 The Authors. International Journal of Food Science and Technology © 2011 Institute of Food Science and Technology.

Perera H.D.S.M.,Industrial Technology Institute ITI | Samarasekera J.K.R.R.,Industrial Technology Institute ITI | Handunnetti S.M.,University of Colombo | Weerasena O.V.D.S.J.,University of Colombo
Industrial Crops and Products | Year: 2016

The search for novel sources of new anti-inflammatory agents has gained an increasing demand with the rising number of indications of inflammatory mediated diseases. Traditionally, medicinal plant extracts have been used to treat number of diseases mediated by inflammation and still remain as potent sources of new anti-inflammatory agents and antioxidants. Therefore, the present study was undertaken to evaluate the in vitro anti-inflammatory and anti-oxidant properties of ten Sri Lankan medicinal plant extracts, traditionally used to treat diseases associated with inflammation. Arachidonate-5-lipoxygenase (A5-LOX), hyaluronidase, xanthine oxidase enzyme inhibitory assays and nitric oxide (NO) production inhibitory assay in lipopolysaccharide activated RAW 264.7 macrophages were used to evaluate in vitro anti-inflammatory activity. Antioxidant activity, total polyphenolic and total flavonoid contents were determined using six standard in vitro assays. The extract of Murraya koenigii L.exhibited the highest anti-A5-LOX activity (IC50 = 7.83 ± 0.42 μg/mL), while that of Symplocos cochinchinesis Lour. showed high anti-hyaluronidase activity (69.35%) along with a high oxygen radical absorbance capacity (ORAC) (1539 ± 31 mg TE/g). The extract of Calophyllum innophyllum L. also exhibited high anti-hyaluronidase activity (68.45% at 500 μg/mL), xanthine oxidase inhibitory activity (60.63% at 250 μg/mL), NO production inhibitory activity (35.00% at 500 μg/mL) along with a marked 2,2-diphenyl-2-picryl-hydrazyl (DPPH) free radical scavenging activity (IC50 = 4.42 ± 0.03 μg/mL) and ferric reducing antioxidant power (FRAP) (8231 ± 5 mg TE/g). Ferrous ion chelating (FIC) activity of plant extracts was found to be low in comparison with the reference standard EDTA-Na2. Higher total polyphenol content (TPC) and total flavonoid content (TFC) were recorded for the extracts of Cyanometra cauliflora L. and Murraya koenigii L. respectively. All bio-activities were found to be significant at p < 0.05. In the correlations analysis, a high positive correlation was observed between anti-A5-LOX and DPPH free radical scavenging activities. Moderate, positive correlations were observed among NO production inhibitory, xanthine oxidase inhibitory activities, FRAP and ORAC of plant extracts. This is the first report demonstrating the anti-A5-LOX, anti-hyaluronidase, xanthine oxidase and nitric oxide production inhibitory activities of ethanol extracts of the tested Sri Lankan medicinal plant extracts. The findings of the study support the traditional uses of these plant extracts against inflammatory mediated diseases. Moreover, the extracts, having promising in vitro anti-inflammatory and antioxidant properties could be effectively used for pharmaceutical, neutraceutical as well as for cosmaceutical applications. © 2016

Abeysekera W.K.S.M.,Industrial Technology Institute ITI | Premakumara G.A.S.,Industrial Technology Institute ITI | Dar A.,University of Karachi | Choudhary M.I.,University of Karachi | And 5 more authors.
Journal of Food Biochemistry | Year: 2015

Extracts, fractions and gastrointestinal-resistant protein hydrolysates (GRPH) from rice bran (RB) of four Sri Lankan traditional varieties were studied for growth inhibition (GI) and cytotoxicity against human lung cancer (NCI-H460), cervical cancer (HeLa) cell lines and effect on glutathione S-transferase (GST) in vitro. RB extracts showed significantly high (P<0.05) GI (GI50μg/mL: HeLa: 19.24±3.47 to 112.58±0.05; NCI-H460: 54.63±4.89 to 170.31±4.71) and cytotoxicity (LC50μg/mL: HeLa: 240.12±9.23 to 476.22±0.05; NCI-H460: 255.36±1.81 to 412.07±17.73) compared with GRPH. Highest GI (GI50: 30.63±9.19 to 44.40±5.34μg/mL) and cytotoxicity (LC50: 141.31±14.47 to 169.51±2.04μg/mL) was evident in nonpolar fractions (dichloromethane: Goda Heeneti and Sudu Heeneti; hexane: Dik Wee and Sudu Heeneti). No cytotoxicity was evident to normal cells. All RB extracts had high GST inhibition. Properties observed indicate its usefulness in managing lung and cervical cancers. Practical Applications: Lung and cervical cancers are two of the most commonly diagnosed cancers in men and women, respectively, and leading causes of mortality. Although numerous potent chemotherapeutic drugs are available for management, most of these drugs are very expensive and are associated with numerous side effects. Further, anticancer drugs containing electrophilic centers can easily form a conjugate with glutathione, which is facilitated by glutathione S-transferase (GST) and excreted from the body resulting in lower efficacy. Therefore, GST inhibitors are used as adjuvants during cancer chemotherapy. The selected rice varieties in this study claimed to possess health-enhancing properties according to Sri Lankan ethnomedicine. The findings of this study clearly showed that bran extracts, fractions and gastrointestinal-resistant protein hydrolysates of selected rice varieties had growth inhibition and cytotoxicity against both lung and cervical cancers. Further, all of these selected varieties had high GST inhibition. Therefore, selected rice brans may be useful in the management of lung and cervical cancers. © 2015 Wiley Periodicals, Inc.

Premakumara G.A.S.,Industrial Technology Institute ITI | Abeysekera W.K.S.M.,Industrial Technology Institute ITI | Ratnasooriya W.D.,University of Colombo | Chandrasekharan N.V.,University of Colombo | Bentota A.P.,Rice Research and Development Center
Journal of Cereal Science | Year: 2013

Brans of 23 traditional and 12 improved (both red and white) rice varieties in Sri Lanka were screened for anti-amylase and anti-glycation activities invitro. Varieties which showed the highest inhibitory activities at screening were further investigated for anti-glucosidase and glycation reversing as anti-diabetic properties. The same varieties were studied for selected antioxidant properties. Significantly high anti-amylase and anti-glycation activities were observed for bran extracts of red varieties compared to white varieties at screening. Traditional red rice varieties, Masuran, Sudu Heeneti, Dik Wee and Goda Heeneti, exhibited significant and dose dependent anti-amylase, anti-glycation and glycation reversing activities. These varieties also showed marked antioxidant properties. It is concluded that brans of Sri Lankan traditional red rice varieties Masuran, Sudu Heeneti, Dik Wee and Goda Heeneti may be potential food supplements for diabetes. © 2013 Elsevier Ltd.

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