Industrial Technology Development Institute

Taguig, Philippines

Industrial Technology Development Institute

Taguig, Philippines

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Chen Y.-L.,National Taiwan University | Yu S.-J.,National Taiwan University | Huang H.-Y.,National Taiwan University | Chang Y.-L.,National Taiwan University | And 7 more authors.
Eukaryotic Cell | Year: 2014

Candida tropicalis, a species closely related to Candida albicans, is an emerging fungal pathogen associated with high mortality rates of 40 to 70%. Like C. albicans and Candida dubliniensis, C. tropicalis is able to form germ tubes, pseudohyphae, and hyphae, but the genes involved in hyphal growth machinery and virulence remain unclear in C. tropicalis. Recently, echinocandinand azole-resistant C. tropicalis isolates have frequently been isolated from various patients around the world, making treatment difficult. However, studies of the C. tropicalis genes involved in drug tolerance are limited. Here, we investigated the roles of calcineurin and its potential target, Crz1, for core stress responses and pathogenesis in C. tropicalis. We demonstrate that calcineurin and Crz1 are required for hyphal growth, micafungin tolerance, and virulence in a murine systemic infection model, while calcineurin but not Crz1 is essential for tolerance of azoles, caspofungin, anidulafungin, and cell wall-perturbing agents, suggesting that calcineurin has both Crz1-dependent and -independent functions in C. tropicalis. In addition, we found that calcineurin and Crz1 have opposite roles in controlling calcium tolerance. Calcineurin serves as a negative regulator, while Crz1 plays a positive role for calcium tolerance in C. tropicalis. © 2014, American Society for Microbiology. All Rights Reserved.


Paglicawan M.A.,Industrial Technology Development Institute | Paglicawan M.A.,Gyeongsang National University | Kim J.K.,Gyeongsang National University | Bang D.-S.,Kumoh National Institute of Technology
Polymer Composites | Year: 2010

An investigation was reported here with an aim to prepare nanocomposite thermoplastic elastomer gels by dissolving polystyrene-b poly(ethylene/butylene) -b-polystyrene (SEBS) triblock copolymer in selective hydrocarbon oils with the presence of multiwalled carbon nanotubes (MWCNTs). The properties related to morphology, viscoelasticity, electrical and mechanical properties, and thermal stability were explored and discussed. Dynamic rheological measurements of the resultant nanocomposite thermoplastic elastomer gels (NCTPEGs) confirmed that addition of MWCNTs affects the linear viscoelastic properties in which dynamic storage and loss moduli increase to some extent. At a temperature between 30°C and 40°C below the gel point the NCTPEGs have dynamic storage modulus greater than loss modulus (G0 and G00), thereby indicating that at room temperature a physical network is still present despite the addition of MWCNTs. The morphological properties revealed that MWCNTs were dispersed and exfoliated within the swollen TPE. The incorporation of small quantity of MWCNTs improved the thermal stability and mechanical properties of NCTPEGs. POLYM. COMPOS., 31:210217, 2010. Copyright © 2009 Society of Plastics Engineers.


Torres R.C.,Industrial Technology Development Institute | Torres R.C.,National Research Council of the Philippines | Garbo A.G.,Industrial Technology Development Institute | Walde R.Z.M.L.,National Research Council of the Philippines
Parasitology Research | Year: 2015

Recent studies regarding the harmful effects of synthetic larvicides initiated the need to investigate for unconventional measures that are environmentally safe and target-specific against Aedes aegypti larvae. Thus, the main objectives of the study are to evaluate the larvicidal toxicity of the solvent fractions of Anacardium occidentale shell wastes against the third and fourth instar larvae of A. aegypti and to compare the results with the commercial larvicide product. The shell wastes were extracted with 95 % EtOH followed by polarity-based fractionation. The fractions were tested for larvicidal activity according to the World Health Organization bioassay method. These were then characterized by quantitative thin-layer chromatographic (TLC) fingerprinting. The hexane fraction gave the strongest activity among the fractions with an LC50 of 4.01 mg/L and LC90 of 11.29 mg/L highly comparable to the commercial larvicide, which exhibited an LC50 of 1.71 mg/L and LC90 of 8.41 mg/L. The dichloromethane fraction exhibited 9.70 mg/L LC50 and 18.44 mg/L LC90. The remarkable toxicity effects exhibited by these fractions indicate their potential to provide core structures from which sustainable and environmentally safe plant-based larvicidal agents can be synthesized. © 2015, Springer-Verlag Berlin Heidelberg.


Torres R.C.,Industrial Technology Development Institute | Torres R.C.,National Research Council of the Philippines | Garbo A.G.,Industrial Technology Development Institute | Walde R.Z.M.L.,National Research Council of the Philippines
Asian Pacific Journal of Tropical Medicine | Year: 2014

Objective: To evaluate the toxicity of the ethanol and hexane extracts of the different parts of Persea americana Mill. (P. americana) toward third and fourth instars larvae of Aedes aegypti (Ae. aegypti) and to characterize the ethanol extract by qualitative phytochemical analysis. Methods: The seeds, peels and pulp of P. americana were processed for crude extraction using 95% ethanol and n-hexane. Crude extracts were bio-assayed for larvicidal activity against Ae. aegypti following the World Health Organization standard bioassay method. The mortality was observed at 24 h and 48 h after treatment and data were subjected to probit analysis to determine lethal concentrations (LC50 and LC90). The ethanol extract was characterized by phytochemical analysis. Results: Both the hexane and ethanol extracts from the different parts of P. americana exhibited evidence of larvicidal toxicity. The hexane extract from the seeds exhibited the highest toxicity with LC50 and LC90 values of 9.82 mg/L and 22.19 mg/L, respectively, while the ethanol seed extract exhibited LC50 of 16.48 mg/L and LC90 45.77 mg/L, respectively. This was closely followed by the ethanol extract of the peels with an LC50 of 10.35 mg/L and LC90 of 26.29 mg/L. The pulp extracted with ethanol also yielded great larvicidal toxicity with LC50 of 21.32 mg/L and LC90 of 59.45 mg/L. Results of the phytochemical analysis of the ethanol seed extract indicated presence of alkaloids, tannins, saponins, unsaturated steroids and triterpenoids, flavonoids (leucoanthocyanins), fats and oils. Conclusions: Both the hexane and ethanol extracts of P. americana showed promising potential as an alternative source of a more sustainable, non-toxic and environmentally friendly solution for the control of dengue vector, Ae. aegypti. © 2014 Hainan Medical College.


PubMed | Industrial Technology Development Institute and National Research Council of the Philippines
Type: | Journal: Asian Pacific journal of tropical medicine | Year: 2014

To evaluate the toxicity of the ethanol and hexane extracts of the different parts of Persea americana Mill. (P. americana) toward third and fourth instars larvae of Aedes aegypti (Ae. aegypti) and to characterize the ethanol extract by qualitative phytochemical analysis.The seeds, peels and pulp of P. americana were processed for crude extraction using 95% ethanol and n-hexane. Crude extracts were bio-assayed for larvicidal activity against Ae. aegypti following the World Health Organization standard bioassay method. The mortality was observed at 24 h and 48 h after treatment and data were subjected to probit analysis to determine lethal concentrations (LC50 and LC90). The ethanol extract was characterized by phytochemical analysis.Both the hexane and ethanol extracts from the different parts of P. americana exhibited evidence of larvicidal toxicity. The hexane extract from the seeds exhibited the highest toxicity with LC50 and LC90 values of 9.82 mg/L and 22.19 mg/L, respectively, while the ethanol seed extract exhibited LC50 of 16.48 mg/L and LC90 45.77 mg/L, respectively. This was closely followed by the ethanol extract of the peels with an LC50 of 10.35 mg/L and LC90 of 26.29 mg/L. The pulp extracted with ethanol also yielded great larvicidal toxicity with LC50 of 21.32 mg/L and LC90 of 59.45 mg/L. Results of the phytochemical analysis of the ethanol seed extract indicated presence of alkaloids, tannins, saponins, unsaturated steroids and triterpenoids, flavonoids (leucoanthocyanins), fats and oils.Both the hexane and ethanol extracts of P. americana showed promising potential as an alternative source of a more sustainable, non-toxic and environmentally friendly solution for the control of dengue vector, Ae. aegypti.


Ebarvia B.S.,Industrial Technology Development Institute | Ubando I.E.,Industrial Technology Development Institute | Sevilla F.B.,University of Santo Tomas of Philippines
Talanta | Year: 2015

The measurement of banned antibiotic like chloramphenicol is significant for customer protection and safety. The presence of residual antibiotics in foods and food products of animal origin could pose as health hazards and affect food quality for global acceptance. In this study, the potential of a chloramphenicol sensor based on molecularly imprinted polymer (MIP) coupled with a piezoelectric quartz crystal was explored. The MIP was prepared by precipitation polymerization at 60 °C. Methacrylic acid was used as monomer, trimethylolpropane trimethacrylate (TRIM) as crosslinker, and chloramphenicol as the template. Template removal on the resulting polymer was done by extraction using methanol-acetic acid. Characterization of the MIP and NIP were conducted by spectroscopic and microscopic methods. These further supported the imprinting and rebinding process of chloramphenicol to the polymer matrix. The chloramphenicol sensor was devised by spin-coating onto one side of the 10 MHz AT-cut quartz crystal the MIP suspension in polyvinylchloride-tetrahydrofuran (6:2:1 w/w/v) solution. Optimization of sensor response was performed by varying the type of cross-linker, amount of MIP sensing layer, curing time, and pH. The sensor exhibited good sensitivity of about 73 Hz/log (conc., μg mL-1) and good repeatability (rsd<10%). A linear relationship (r2=0.9901) between frequency shift and chloramphenicol concentration in the range of 1×10-6 up to 1×10-1 μg/mL was obtained. The sensor response was highly selective to chloramphenicol than with other compounds of similar chemical structures. Acceptable percent recovery was obtained for real sample analysis using the sensor. The proposed sensor could be a promising low cost and highly sensitive approach for residual chloramphenicol quantification in food products. © 2015 Elsevier B.V. All rights reserved.


Negishi N.,Japan National Institute of Advanced Industrial Science and Technology | Sano T.,Japan National Institute of Advanced Industrial Science and Technology | Hirakawa T.,Japan National Institute of Advanced Industrial Science and Technology | Koiwa F.,Japan National Institute of Advanced Industrial Science and Technology | And 3 more authors.
Applied Catalysis B: Environmental | Year: 2012

Photocatalytic degradation of organophosphorus compounds including organophosphonic and organophosphinic acids by TiO2 immobilized silica gel in a water phase was carried out. Photocatalytic degradation of parent organophosphorus compounds and formation of intermediates were observed and the photocatalytic degradation scheme for each pesticide is proposed. Overall, the photocatalytic degradation of organophosphorus compounds in aqueous phase was estimated to form organophosphoric (organophosphonic and organophosphinic) acids as intermediates of the reaction. These organophosphoric acid intermediates were selectively adsorbed onto TiO2 surface and as a result, elution of these compounds into the aqueous phase was not observed. Similarly, after photocatalytic degradation, H3PO4 was either not observed or slightly observed as the final product. Likewise, rapid adsorption of the studied parent organophosphoric acids on TiO2 surface resulted in significant reduction in the concentration of these compounds even under dark condition. Upon UV irradiation, total organic carbon (TOC) level increased, and this is indicating the elution of some organic intermediates into the aqueous phase. However, these organic intermediates were eventually degraded with the UV irradiation. © 2012 Elsevier B.V.


Ongo E.,Industrial Technology Development Institute | Ongo E.,University of Santo Tomas of Philippines | Falasconi M.,University of Santo Tomas of Philippines | Sberveglieri G.,University of Santo Tomas of Philippines | And 5 more authors.
Procedia Engineering | Year: 2012

This study presents a practical and promising approach to profile the headspace aroma attributes of Philippine civet coffee using electronic nose (E-nose) and gas chromatography mass spectrometry (GCMS). Chemometric pattern method was applied to enhance the discrimination of civet coffee against its control coffee beans (not eaten by civet animal). E-nose analysis revealed that aroma characteristic is one of the most important quality indicators of civet coffee. The result was supported by GCMS analysis. The chromatographic fingerprints indicated that civet coffee differed with their control beans in terms of composition and concentration of individual volatile constituents. Chemometric discrimination of E-nose and GCMS data demonstrated a clearly separated civet from their control coffees indicating that cultivar and geographic origins dictate the aroma and volatiles variations in coffee. © 2012 The Authors. Published by Elsevier Ltd.


PubMed | Industrial Technology Development Institute and University of Santo Tomas of Philippines
Type: | Journal: Talanta | Year: 2015

The measurement of banned antibiotic like chloramphenicol is significant for customer protection and safety. The presence of residual antibiotics in foods and food products of animal origin could pose as health hazards and affect food quality for global acceptance. In this study, the potential of a chloramphenicol sensor based on molecularly imprinted polymer (MIP) coupled with a piezoelectric quartz crystal was explored. The MIP was prepared by precipitation polymerization at 60 C. Methacrylic acid was used as monomer, trimethylolpropane trimethacrylate (TRIM) as crosslinker, and chloramphenicol as the template. Template removal on the resulting polymer was done by extraction using methanol-acetic acid. Characterization of the MIP and NIP were conducted by spectroscopic and microscopic methods. These further supported the imprinting and rebinding process of chloramphenicol to the polymer matrix. The chloramphenicol sensor was devised by spin-coating onto one side of the 10 MHz AT-cut quartz crystal the MIP suspension in polyvinylchloride-tetrahydrofuran (6:2:1 w/w/v) solution. Optimization of sensor response was performed by varying the type of cross-linker, amount of MIP sensing layer, curing time, and pH. The sensor exhibited good sensitivity of about 73 Hz/log (conc., g mL(-1)) and good repeatability (rsd<10%). A linear relationship (r(2)=0.9901) between frequency shift and chloramphenicol concentration in the range of 110(-6) up to 110(-1) g/mL was obtained. The sensor response was highly selective to chloramphenicol than with other compounds of similar chemical structures. Acceptable percent recovery was obtained for real sample analysis using the sensor. The proposed sensor could be a promising low cost and highly sensitive approach for residual chloramphenicol quantification in food products.


PubMed | Industrial Technology Development Institute
Type: Journal Article | Journal: Parasitology research | Year: 2015

Recent studies regarding the harmful effects of synthetic larvicides initiated the need to investigate for unconventional measures that are environmentally safe and target-specific against Aedes aegypti larvae. Thus, the main objectives of the study are to evaluate the larvicidal toxicity of the solvent fractions of Anacardium occidentale shell wastes against the third and fourth instar larvae of A. aegypti and to compare the results with the commercial larvicide product. The shell wastes were extracted with 95% EtOH followed by polarity-based fractionation. The fractions were tested for larvicidal activity according to the World Health Organization bioassay method. These were then characterized by quantitative thin-layer chromatographic (TLC) fingerprinting. The hexane fraction gave the strongest activity among the fractions with an LC50 of 4.01 mg/L and LC90 of 11.29 mg/L highly comparable to the commercial larvicide, which exhibited an LC50 of 1.71 mg/L and LC90 of 8.41 mg/L. The dichloromethane fraction exhibited 9.70 mg/L LC50 and 18.44 mg/L LC90. The remarkable toxicity effects exhibited by these fractions indicate their potential to provide core structures from which sustainable and environmentally safe plant-based larvicidal agents can be synthesized.

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