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Pal P.K.,Industrial Research Institute of Himalayan Bioresource Technology | Agnihotri V.K.,Industrial Research Institute of Himalayan Bioresource Technology | Gopichand,Industrial Research Institute of Himalayan Bioresource Technology | Deosharan Singh R.,Industrial Research Institute of Himalayan Bioresource Technology
Industrial Crops and Products | Year: 2014

Pruning has been implicated as a promoter of bud outgrowth or shoot branching and flower yield in Rosa damascena Mill., but the extent and time of pruning are tightly regulated by the climatic conditions of the growing region. In western Himalaya region in India, the effect of extent and time of pruning on flower yield and secondary metabolites of R. damascena is still unsolved. Thus, a field experiment comprising three levels of pruning and four different time of pruning was conducted to confirm that the higher yield and quality can be achieved through the standardization of pruning practices. Principal component analysis showed that pruning at 90cm height from ground level (FGL) on 15th December is preferable for higher flower yield and essential oil. The yield data suggest that moderate pruning (90cm FGL) leads to an increased rate of flower production (11.33 and 13.22gnew shoot-1). Pruning on15th December produced 10.6-42.77% higher flower yield compared with pruning on 31st October. The quality of oil is considerably affected by level and time of pruning. The results have suggested that the higher yield and quality of R. damascena can be achieved through the standardization of pruning practices. © 2013 Elsevier B.V. Source


Walia Y.,Industrial Research Institute of Himalayan Bioresource Technology | Dhir S.,Industrial Research Institute of Himalayan Bioresource Technology | Ram R.,Industrial Research Institute of Himalayan Bioresource Technology | Zaidi A.A.,Industrial Research Institute of Himalayan Bioresource Technology | Hallan V.,Industrial Research Institute of Himalayan Bioresource Technology
Plant Pathology | Year: 2014

Apple scar skin viroid (ASSVd) is the type member of the genus Apscaviroid of the family Pospiviroidae and is known to cause scarring and dappling of apple fruits. The herbaceous host range of ASSVd was identified using an infectious dimeric clone. Among 13 plant species inoculated with the viroid, nine were found to be systemic hosts and three served as indicator hosts. The viroid could be transferred to these hosts through agroinoculation, rubbing of dimeric RNA transcripts, dimeric DNA plasmids and through sap inoculation. Single-strand confirmation polymorphism (SSCP) analysis of the progeny obtained after inoculation of the infectious ASSVd clone showed the presence of four sequence variants (V1, V2, V3 and V4) which was also confirmed by sequencing. Two sequence variants (V1 and V3) were identified as dominant, representing 47·72 and 36·36% of the total progeny, respectively. The V4 sequence variant was similar to the wild type and occurred at a frequency of only 4·5% of the total progeny. © 2013 British Society for Plant Pathology. Source

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