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Prihatini R.,Indonesian Tropical Fruit Research Institute | Ihsan F.,Indonesian Tropical Fruit Research Institute | Indriyani N.L.P.,Indonesian Tropical Fruit Research Institute
Journal of Horticultural Research | Year: 2016

The molecular analysis of 32 durian F1 hybrids, resulted from crossing of the Arp 8990 (female parent) and â € Otong' (male parent), was conducted in order to determine the genetic characteristics of hybrids and parents, as it would be followed/evidenced by the variability of traits produced from the cross breeding. The RAPD analyses of 14 primers resulted in 114 scoring bands, 112 (98.2%) of them were polymorphic, with 4 to 11 bands amplified per primer. The electrophoresis gel of the PCR results revealed that some hybrids produced different band patterns compared to the parents; this indicated the crossing between parents' alleles and trait combinations from both the parents. The Dice-Sorensen similarity coefficient demonstrated that most of the hybrids had distant genetic similarities with both parents, which were ranged from 0.141 [71B(4) and 72B(15)] to 0.776 [71B(15) and 48B(1)]. The UPGMA method was used to construct the dendrogram, which grouped the hybrids in five clusters with distinct genetic relationships and was confirmed with the PCA analysis. This result implied that above crossing produced hybrids having characters different from the parents. © 2016 Riry Prihatini et al., published by De Gruyter Open.


Daniells J.W.,Economic Development and Innovation | O'Neill W.T.,Economic Development and Innovation | Hermanto C.,Indonesian Tropical Fruit Research Institute | Ploetz R.C.,University of Florida
Acta Horticulturae | Year: 2011

Genetically related populations of Fusarium oxysporum f. sp. cubense (Foc) are defined by vegetative compatibility groups (VCGs). In 2006, the Queensland Department of Employment, Economic Development and Innovation (Qld DEEDI), the Indonesian government and Bioversity International commenced an Australian Centre for International Agricultural Research (ACIAR) project which included a study of the geographical distribution of Foc in Indonesia. Relationships that existed between different banana varieties and Foc VCGs were also assessed. We consulted two global databases that contain records of Foc disease samples: the Qld DEEDI database and that maintained at the University of Florida in Homestead. Information from these sources suggested that: (i) Most varieties are susceptible to tropical race 4 (TR4) (VCG 01213); (ii) 'Gros Michel' (AAA), 'Silk' (AAB) and 'Saba' (ABB) are each susceptible to several VCGs; (iii) Some varieties tend to have certain VCGs associated with them; and (iv) 'Sucrier' (AA) and 'Lakatan' (AAA) are only affected by TR4. Although a greater understanding of these relationships would require pathogenicity studies with representative isolates of the different VCGs, these observations reinforce prior concerns over VCG 01213 and its continued spread in Asia. Future collections of this pathogen should be accompanied by photos of the sample plant to ensure that varieties are identified correctly.


Herwina H.,Andalas University | Nasir N.,Andalas University | Jumjunidang,Indonesian Tropical Fruit Research Institute | Yaherwandi,Andalas University
Asian Myrmecology | Year: 2013

A brief study on ant species on banana plants with Banana Bunchy-top Virus (BBTV) symptoms was conducted by direct collection in four regencies and one city of West Sumatra Province in Indonesia. During the sampling we found 39 banana plants with BBTV symptoms, of which36 were occupied by insects, and of these 35 plants contained ants. A total of 24 species of ants, belonging to 16 genera, were collected. Myrmicinae was the subfamily with the highest number of species (11), followed by Formicinae (seven) and Dolichoderinae (six). Tetramorium and Technomyrmex were the genera with the most species (four). Dolichoderus thoracicus was found most frequently among samples, accounting for16% of species occurrences, followed by Tapinoma melanocephalum and Paratrechina longicornis (11 and 10% respectively). Seventeen species of ants were found associated with aphids of which six showed a statistically-significant association, while seven ant species were not observed with trophobionts. Species that were found more often associated with aphids were found more frequently during the study. © Henny Herwina, Nasril Nasir, Jumjunidang and Yaherwandi.


Nofiarli,Indonesian Tropical Fruit Research Institute | Anwar K.,Andalas University | Hazli N.,Andalas University
WSEAS Transactions on Biology and Biomedicine | Year: 2014

The research was conducted in the Pilot Plant Laboratory, Faculty of Agriculture Technology, Andalas University, Padang, West Sumatra, Indonesia in October 2008 to February 2009. The research objective was to know biodiesel characteristic of kepayang seed oil. The biodiesel was made in two steps i.e., esterification process and transesterification. Physicochemical characteristic of the biodiesel were yield, viscosity, density, acid number, Iod number, saponification number, gliserol number, and ester content. Biodiesel from kepayang oil toward to viscosity, density, acid number, iod number, soup number, gliserol number, and ester content was full fill ASTM biodiesel quality. The result shown that kepayang biodiesel can be used for diesel engine to substitute the petro diesel.


Mansyah E.,Indonesian Tropical Fruit Research Institute | Muas I.,Indonesian Tropical Fruit Research Institute | Jawal A.S.M.,Indonesian Tropical Fruit Research Institute | Sobir,Bogor Agricultural University
Sabrao Journal of Breeding and Genetics | Year: 2010

Indonesia is one of the most important mangosteen producing countries whose the growing areas are scattered on almost all of the islands. Mangosteen variability was observed based on field expeditions from 1992 to 2005. The data were collected on morphological observations on mangosteen production centres in West Sumatra, Riau, Jambi, South Sumatra, Bengkulu, Bangka/Belitung, West Java, Central Java, and East Java based on a total number of 192 mangosteen trees. The objectives of the research were to find out the variability of Indonesian mangosteen based on the morphological characters and to determine the morphological characters that can be used to distinguish mangosteen accessions. Leaves and mature fruits were observed for morphological variations. The results show that mangosteens vary in their morphological appearances. Among the morphological variations obtained, there are 11 specific characters that can be used to distinguish the apomictic mangosteen. These characters are canopy shape, mature leaf colour, number of flowers and fruits per cluster, pedicel length, fruit shape, fruit-base shape, stigma lobe shape, size, and thickness, the number of fruit segments, and rind thickness. Based on the morphological characters the mangosteen in Sumatra and Java can be divided into seven kinds. This information will be useful for researchers and will make the task of the curators easier in the area of mangosteen germplasm evaluation.


Sutanto A.,Bogor Agricultural University | Sutanto A.,Indonesian Tropical Fruit Research Institute | Sukma D.,Bogor Agricultural University | Hermanto C.,Indonesian Tropical Fruit Research Institute | Sudarsono S.,Bogor Agricultural University
Emirates Journal of Food and Agriculture | Year: 2014

Isolation and characterization of resistance genes from local banana cultivars was important in order to support the development of FOC resistant banana cultivars. Resistance gene analogues (RGAs) were isolated and characterized form three fusarium resistant banana cultivars using degenerate primers based on NBS domains. From 91 fragments sequenced, 17 fragments were positively NBS-type sequences and encoded as MNBS1-MNBS17. Phylogenetic analysis of MNBS deduced amino classified into three groups; the first group consisted of 14 members (MNBS1-MNBS14) with 97.4% identity, and the other three groups consisted of one member (MNBS15, MNBS16 and MNBS17, respectively) with 28.5% identity. All MNBS sequences were categorized as non-TIR-NBS-LRR. Comparison and phylogenetic analysis of MNBS with other known RGA and R genes showed that deduced amino acid MNBSs shared 91.7-98.8% identity with Musa NBS-LRR and 19.9-35.5% identity with known R genes. Among them, MNBS17 shared 50.5% identity with RGC2 (ABY75802) that assosiated to FOC race 4 resistant Musa species.


Santoso P.J.,Bandung Institute of Technology | Santoso P.J.,Indonesian Tropical Fruit Research Institute | Aryantha I.N.P.,Bandung Institute of Technology | Pancoro A.,Bandung Institute of Technology | Suhandono S.,Bandung Institute of Technology
Asian Journal of Plant Pathology | Year: 2015

Phytophthora and Pythium are reported as pathogen causing tree-decline to durian. Survey relating to their diversity and distribution in Indonesia is very limited. A research to identify their molecular, morphological characteristic and distribution was undertaken from 2011 to 2014. Molecular identification was based on ITS-nrDNA sequences. Morphological characteristics observed were colony motif, shape and sporangium size. A total of 36 isolates were successfully baited from 32 durian fields represent of 17 provinces in Indonesia. Based on ITS-nrDNA sequences, the isolates correspond to six Pythiaceae species, namely Pythium cucurbitacearum, Pythium vexans, Pythium sp. D37, Pythium deliense, Phytophthora cinnamomi var., parvispora and Phytophthora palmivora. These species demonstrated the diversity of Pythiaceae associated with durian in Indonesia. The diversity was also confirmed by the morphological characteristics such as colony motif, shape and sporangium size. Pythium cucurbitacearum were found in 13 (76.5%) provinces and Pythium vexans were in 10 (58.8%). The findings concerning the distribution of both pathogens are indicating that these two species could be more dangerous than Phytophthora palmivora. This is the first time; Pythium cucurbitacearum, Pythium sp., D37, Pythium deliense, Phytophthora cinnamomi var., parvispora reported their association with durian. © 2015 Academic Journals Inc.


Budiyanti T.,Indonesian Tropical Fruit Research Institute | Hadiati S.,Indonesian Tropical Fruit Research Institute | Prihatini R.,Indonesian Tropical Fruit Research Institute | Sobir,University of Indonesia
International Journal on Advanced Science, Engineering and Information Technology | Year: 2015

Indonesia is one of the megabiodivesity, which is rich with germplasms including tropical fruit. Snake fruit (Salacca spp.) is a native fruit of Indonesia with a scaly peel and sweet-tart taste. The genetic diversity of 17 accessions of Indonesian snake fruit was resolved using the Random Amplified Polymorphic DNA Polymerase Chain Reaction with 5 primers. The study demonstrated that the samples were grouped in six different clusters with coefficient of similarity ranged from 0.12 to 0.71. The value indicated the wide range of genetic variability among the tested plants. This variability was an important resources for the snake fruit breeding program in developing the consumer's preferred product which by the end supports the plant diversification program.


Hermanto C.,Indonesian Tropical Fruit Research Institute | Eliza,Indonesian Tropical Fruit Research Institute | Emilda D.,Indonesian Tropical Fruit Research Institute
Acta Horticulturae | Year: 2013

Fusarium wilt has become a critical and crucial issue in world banana production due to it being a deadly disease that has caused significant losses for many banana industries around the world. The objective of this research was to find an environmentally friendly control measure through enhanced soil suppression of fusarium wilt disease. The research was carried out in Batu Sangkar, West Sumatera, Indonesia in 2006-2007, on a site with natural inoculation of Fusarium oxysporum f. sp. cubense VCG 01213/16. The experiment used the 'Ambon Hijau' (Cavendish type) cultivar, arranged in randomized block design with three replications and 20 plants per replicate. The treatments were A) Gliostar (a formulated Gliocladium), B) M-Rif (a formulated organic material), C) Trichoderma (check plot, biocontrol that is commonly used by farmers), D) Gliostar + M-Rif, E) Gliostar + Trichoderma, F) Trichoderma + M-Rif, G) Gliostar + Trichoderma + M-Rif, H) Control. The results showed that all of the treatments reduced Fusarium propagules in the soil, but did not impact on Fusarium wilt incidence unless organic material was added. © 2013 ISHS.


Nasution F.,Indonesian Tropical Fruit Research Institute | Hadiati S.,Indonesian Tropical Fruit Research Institute
Agrivita | Year: 2014

Guava has several different accessions. Guava diversity needs to be studied and evaluated in order to determine the next steps in the guava breeding. The objective of this research was to characterize and cluster some guava germplasm collections. The study was conducted at Aripan and Subang experimental farm, Indonesian Tropical Fruit Research Institute from January 2012 to December 2012. Five fruits of each accession were randomly selected, sampled, and then characterized using UPOV guidelines. Obtained data were analyzed by NTSYS ver.2.1. The Similarity level of 19 accessions ranging from 70-90% or the genetic distance was between 0-20%. Dendogram obtained could be clustered into two different groups, namely group I (ARP9406, ARP9407, ARP8653, ARP8742, ARP10.2, JBT001, JBT002, ARP8740, JBT003andJBT004) and group II (ARP10.7, ARP10.6, ARP10.1, ARP10.12, ARP10.9, ARP10.11, ARP8744, ARP8741 and ARP8743). The result of this research can be used for guava breeding. Species diversity and genetic resources are very important to produce new varieties. This is expected to be highly valuable in the future. © 2014, Agriculture Faculty Brawijaya University. All rights reserved.

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