Indonesian Tropical Fruit Research Institute

Solok, Indonesia

Indonesian Tropical Fruit Research Institute

Solok, Indonesia
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Prihatini R.,Indonesian Tropical Fruit Research Institute | Ihsan F.,Indonesian Tropical Fruit Research Institute | Indriyani N.L.P.,Indonesian Tropical Fruit Research Institute
Journal of Horticultural Research | Year: 2016

The molecular analysis of 32 durian F1 hybrids, resulted from crossing of the Arp 8990 (female parent) and â € Otong' (male parent), was conducted in order to determine the genetic characteristics of hybrids and parents, as it would be followed/evidenced by the variability of traits produced from the cross breeding. The RAPD analyses of 14 primers resulted in 114 scoring bands, 112 (98.2%) of them were polymorphic, with 4 to 11 bands amplified per primer. The electrophoresis gel of the PCR results revealed that some hybrids produced different band patterns compared to the parents; this indicated the crossing between parents' alleles and trait combinations from both the parents. The Dice-Sorensen similarity coefficient demonstrated that most of the hybrids had distant genetic similarities with both parents, which were ranged from 0.141 [71B(4) and 72B(15)] to 0.776 [71B(15) and 48B(1)]. The UPGMA method was used to construct the dendrogram, which grouped the hybrids in five clusters with distinct genetic relationships and was confirmed with the PCA analysis. This result implied that above crossing produced hybrids having characters different from the parents. © 2016 Riry Prihatini et al., published by De Gruyter Open.


Murni P.,University of Jambi | Syamsuardi,Andalas University | Nurainas,Andalas University | Mansyah E.,Indonesian Tropical Fruit Research Institute | Chairul,Andalas University
Der Pharmacia Lettre | Year: 2016

Duku 'Kumpeh' is one of the potential local type cultivars of Duku Lansium parasiticum (Osbeck) K.C. Sahni & Bennet.), from specific area in the Jambi. For sustainable utilization of this local germplasm of tropical fruit, understanding of reproduction and genetic information of the plant is needed. The reproductive system of a plant species is a factor that directly determine the potential and success of conservation germplasm for the future. Until now, the breeding system about this plant still unclear although there were different opinions whether duku produce asexually (apomictic), sexual or both. Based on this fact, it is necessary to clarify this problem. A total of 60 seeds from three parental trees (20 seeds from Kumpeh and 22 seeds from Dusun Tuo and 18 seeds from Muaro Panco) were germinated in the greenhouse for obtaining DNA samples. DNA samples were extracted using CTAB method and amplified using Inter Simple Sequences Repeat (ISSR) primers. Four selected primers: (CCT)8 (HQ200181), (AAT)10 (HQ200186), (AAG)6 (HQ200182) and (AG)10 (DQ453906) were used to amplify all DNA samples and estimate the magnitude of genetic variability and out-crossing rate. Based on analysis of genetic variability and assessment of level out-crossing rate of three parental and sixty progenies from three populations proved that local cultivar 'Kumpeh' were facultative apomictic breeding system. The existing identical of all progenies in Kumpeh population with their parental were very important for sustainably utilization of this local type duku 'Kumpeh'. The detection genetic variability (even with low level) at Dusun Tuo and Muaro Panco population were very useful and strategic for genetic conservation and genetic improvement of these germplasm.


Santoso P.J.,Indonesian Tropical Fruit Research Institute | Pancoro A.,Bandung Institute of Technology | Suhandono S.,Bandung Institute of Technology | Aryantha I.N.P.,Bandung Institute of Technology
Agrivita | Year: 2017

Simple sequence repeats have been proved as powerful markers and widely used in molecular breeding to reduce cycles and cost efective. The availability of the marker is, however, very limited in durian. This research aimed to develop SSR markers from durian genomic library. Genomic DNA was isolated from durian shoot leaf, whilst SSR motifs were isolated using membrane-based oligonucleotide enrichment hybridization protocol. Annotation made on the library found 527 unique motifs from 354 durian libraries which form 425 loci. The SSR motifs obtained were generally short repeats which reached 89.6 %, whilst longer repeats were found consisted of compound motifs. Eleven loci were selected as representative for further test to prove their informativity. A number of unique allels were successfully amplified from 17 durian genomes. The analysis showed the polymorphic information content (PIC) values ranged from 0.000 to 0.662 with an average of 0.390. The SSR loci also showed their ability to be used for durian diversity analysis as the evident that the loci could be used as genetic markers for assisting further durian breeding program. © 2017, Agriculture Faculty Brawijaya University. All rights reserved.


Daniells J.W.,Economic Development and Innovation | O'Neill W.T.,Economic Development and Innovation | Hermanto C.,Indonesian Tropical Fruit Research Institute | Ploetz R.C.,University of Florida
Acta Horticulturae | Year: 2011

Genetically related populations of Fusarium oxysporum f. sp. cubense (Foc) are defined by vegetative compatibility groups (VCGs). In 2006, the Queensland Department of Employment, Economic Development and Innovation (Qld DEEDI), the Indonesian government and Bioversity International commenced an Australian Centre for International Agricultural Research (ACIAR) project which included a study of the geographical distribution of Foc in Indonesia. Relationships that existed between different banana varieties and Foc VCGs were also assessed. We consulted two global databases that contain records of Foc disease samples: the Qld DEEDI database and that maintained at the University of Florida in Homestead. Information from these sources suggested that: (i) Most varieties are susceptible to tropical race 4 (TR4) (VCG 01213); (ii) 'Gros Michel' (AAA), 'Silk' (AAB) and 'Saba' (ABB) are each susceptible to several VCGs; (iii) Some varieties tend to have certain VCGs associated with them; and (iv) 'Sucrier' (AA) and 'Lakatan' (AAA) are only affected by TR4. Although a greater understanding of these relationships would require pathogenicity studies with representative isolates of the different VCGs, these observations reinforce prior concerns over VCG 01213 and its continued spread in Asia. Future collections of this pathogen should be accompanied by photos of the sample plant to ensure that varieties are identified correctly.


Nofiarli,Indonesian Tropical Fruit Research Institute | Anwar K.,Andalas University | Hazli N.,Andalas University
WSEAS Transactions on Biology and Biomedicine | Year: 2014

The research was conducted in the Pilot Plant Laboratory, Faculty of Agriculture Technology, Andalas University, Padang, West Sumatra, Indonesia in October 2008 to February 2009. The research objective was to know biodiesel characteristic of kepayang seed oil. The biodiesel was made in two steps i.e., esterification process and transesterification. Physicochemical characteristic of the biodiesel were yield, viscosity, density, acid number, Iod number, saponification number, gliserol number, and ester content. Biodiesel from kepayang oil toward to viscosity, density, acid number, iod number, soup number, gliserol number, and ester content was full fill ASTM biodiesel quality. The result shown that kepayang biodiesel can be used for diesel engine to substitute the petro diesel.


Mansyah E.,Indonesian Tropical Fruit Research Institute | Muas I.,Indonesian Tropical Fruit Research Institute | Jawal A.S.M.,Indonesian Tropical Fruit Research Institute | Sobir,Bogor Agricultural University
Sabrao Journal of Breeding and Genetics | Year: 2010

Indonesia is one of the most important mangosteen producing countries whose the growing areas are scattered on almost all of the islands. Mangosteen variability was observed based on field expeditions from 1992 to 2005. The data were collected on morphological observations on mangosteen production centres in West Sumatra, Riau, Jambi, South Sumatra, Bengkulu, Bangka/Belitung, West Java, Central Java, and East Java based on a total number of 192 mangosteen trees. The objectives of the research were to find out the variability of Indonesian mangosteen based on the morphological characters and to determine the morphological characters that can be used to distinguish mangosteen accessions. Leaves and mature fruits were observed for morphological variations. The results show that mangosteens vary in their morphological appearances. Among the morphological variations obtained, there are 11 specific characters that can be used to distinguish the apomictic mangosteen. These characters are canopy shape, mature leaf colour, number of flowers and fruits per cluster, pedicel length, fruit shape, fruit-base shape, stigma lobe shape, size, and thickness, the number of fruit segments, and rind thickness. Based on the morphological characters the mangosteen in Sumatra and Java can be divided into seven kinds. This information will be useful for researchers and will make the task of the curators easier in the area of mangosteen germplasm evaluation.


Sutanto A.,Bogor Agricultural University | Sutanto A.,Indonesian Tropical Fruit Research Institute | Sukma D.,Bogor Agricultural University | Hermanto C.,Indonesian Tropical Fruit Research Institute | Sudarsono S.,Bogor Agricultural University
Emirates Journal of Food and Agriculture | Year: 2014

Isolation and characterization of resistance genes from local banana cultivars was important in order to support the development of FOC resistant banana cultivars. Resistance gene analogues (RGAs) were isolated and characterized form three fusarium resistant banana cultivars using degenerate primers based on NBS domains. From 91 fragments sequenced, 17 fragments were positively NBS-type sequences and encoded as MNBS1-MNBS17. Phylogenetic analysis of MNBS deduced amino classified into three groups; the first group consisted of 14 members (MNBS1-MNBS14) with 97.4% identity, and the other three groups consisted of one member (MNBS15, MNBS16 and MNBS17, respectively) with 28.5% identity. All MNBS sequences were categorized as non-TIR-NBS-LRR. Comparison and phylogenetic analysis of MNBS with other known RGA and R genes showed that deduced amino acid MNBSs shared 91.7-98.8% identity with Musa NBS-LRR and 19.9-35.5% identity with known R genes. Among them, MNBS17 shared 50.5% identity with RGC2 (ABY75802) that assosiated to FOC race 4 resistant Musa species.


Budiyanti T.,Indonesian Tropical Fruit Research Institute | Hadiati S.,Indonesian Tropical Fruit Research Institute | Prihatini R.,Indonesian Tropical Fruit Research Institute | Sobir,University of Indonesia
International Journal on Advanced Science, Engineering and Information Technology | Year: 2015

Indonesia is one of the megabiodivesity, which is rich with germplasms including tropical fruit. Snake fruit (Salacca spp.) is a native fruit of Indonesia with a scaly peel and sweet-tart taste. The genetic diversity of 17 accessions of Indonesian snake fruit was resolved using the Random Amplified Polymorphic DNA Polymerase Chain Reaction with 5 primers. The study demonstrated that the samples were grouped in six different clusters with coefficient of similarity ranged from 0.12 to 0.71. The value indicated the wide range of genetic variability among the tested plants. This variability was an important resources for the snake fruit breeding program in developing the consumer's preferred product which by the end supports the plant diversification program.


Hermanto C.,Indonesian Tropical Fruit Research Institute | Eliza,Indonesian Tropical Fruit Research Institute | Emilda D.,Indonesian Tropical Fruit Research Institute
Acta Horticulturae | Year: 2013

Fusarium wilt has become a critical and crucial issue in world banana production due to it being a deadly disease that has caused significant losses for many banana industries around the world. The objective of this research was to find an environmentally friendly control measure through enhanced soil suppression of fusarium wilt disease. The research was carried out in Batu Sangkar, West Sumatera, Indonesia in 2006-2007, on a site with natural inoculation of Fusarium oxysporum f. sp. cubense VCG 01213/16. The experiment used the 'Ambon Hijau' (Cavendish type) cultivar, arranged in randomized block design with three replications and 20 plants per replicate. The treatments were A) Gliostar (a formulated Gliocladium), B) M-Rif (a formulated organic material), C) Trichoderma (check plot, biocontrol that is commonly used by farmers), D) Gliostar + M-Rif, E) Gliostar + Trichoderma, F) Trichoderma + M-Rif, G) Gliostar + Trichoderma + M-Rif, H) Control. The results showed that all of the treatments reduced Fusarium propagules in the soil, but did not impact on Fusarium wilt incidence unless organic material was added. © 2013 ISHS.


Nasution F.,Indonesian Tropical Fruit Research Institute | Hadiati S.,Indonesian Tropical Fruit Research Institute
Agrivita | Year: 2014

Guava has several different accessions. Guava diversity needs to be studied and evaluated in order to determine the next steps in the guava breeding. The objective of this research was to characterize and cluster some guava germplasm collections. The study was conducted at Aripan and Subang experimental farm, Indonesian Tropical Fruit Research Institute from January 2012 to December 2012. Five fruits of each accession were randomly selected, sampled, and then characterized using UPOV guidelines. Obtained data were analyzed by NTSYS ver.2.1. The Similarity level of 19 accessions ranging from 70-90% or the genetic distance was between 0-20%. Dendogram obtained could be clustered into two different groups, namely group I (ARP9406, ARP9407, ARP8653, ARP8742, ARP10.2, JBT001, JBT002, ARP8740, JBT003andJBT004) and group II (ARP10.7, ARP10.6, ARP10.1, ARP10.12, ARP10.9, ARP10.11, ARP8744, ARP8741 and ARP8743). The result of this research can be used for guava breeding. Species diversity and genetic resources are very important to produce new varieties. This is expected to be highly valuable in the future. © 2014, Agriculture Faculty Brawijaya University. All rights reserved.

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