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Shaikh R.U.,Poona College | Shaikh R.U.,Swami Ramanand Teerth Marathwada University | Pund M.M.,Indira Gandhi Sr College | Gacche R.N.,Swami Ramanand Teerth Marathwada University
Journal of Traditional and Complementary Medicine | Year: 2015

The present study was carried out to evaluate invivo and invitro anti-inflammatory potential of selected medicinal plants used in Indian traditional medication. The sequentially extracted plant samples as, Cissus quadrangularis, Plumbago zeylanica, Terminalia bellarica and Terminalia chebula in water, ethanol and hexane were evaluated in-vitro for COX-1 and 2 inhibitory and antioxidant activities. The invivo anti-inflammatory activity of selected samples showing promising COX-2 inhibition was assessed using carrageenan and Phorbol Myristate Acetate (PMA) induced mice edema animal model. The results obtained reveals that most of the plants were found to inhibit COX-2 activity as compared to COX-1. It was observed that the extracts of T.bellarica (73.34 %) and T.chebula (74.81 %) showed significant COX-2 selective inhibition as compared to other samples. The ethanol extract of the selected plants demonstrated effective DPPH, OH and superoxide radical scavenging activity. Invivo anti-inflammatory study shows that, T.bellarica and T.chebulla had a significant impact on inhibition of edema formation. The cytotoxicity evaluation study of ethanolic fraction of selected medicinal plants indicates that the selected samples have no effect on cell viability. HPTLC fingerprint of flavonoids of the selected samples was also prepared as a measure of quality control. The results obtained may be useful in strengthening the standardization of the selected botanicals. Moreover the selected plants can be considered as a resource for searching novel anti-inflammatory agents possessing COX-2 inhibition. © 2015 Center for Food and Biomolecules, National Taiwan University. Source


Tawde K.V.,Swami Ramanand Teerth Marathwada University | Gacche R.N.,Swami Ramanand Teerth Marathwada University | Pund M.M.,Indira Gandhi Sr College
Asian Pacific Journal of Tropical Disease | Year: 2012

Objective: To evaluate different solvent extracts of selected Indian traditional medicinal plant against Mycobacterium tuberculosis, its antioxidant potential and cytotoxicity. Methods: Acacia catechu (L.) Willd (Root extract) and Ailanthus excelsa Roxb., leaf extracts of Aegle marmelos Corr., Andrographis paniculata Nees. and Datura metel L. were sequentially extracted in water, ethanol, chloroform and hexane and evaluated for their anti-tuberculosis (TB) activity against Mycobacterium tuberculosis using agar diffusion assay. The zone of inhibition (at 20 and 40 mg/ml) was measured and MIC were calculated. The results were compared with Rifampicin as a standard anti TB drug. The extracts were also evaluated for DPPH and OH radical scavenging activities to understand their antioxidant potential. MTT based cytotoxicity assay was used for evaluating cytotoxicity of the selected samples against Chang liver cells. Results: The selected botanicals were sequentially extracted in water, ethanol, chloroform and hexane and tested for growth inhibition of M. tuberculosi. The hexane extract of A. catechu root and ethanol extract of A. paniculata leaf showed promising activity against M. tuberculosis while remaining extracts showed moderate anti TB activity. The samples were found to possess considerable DPPH and OH radical scavenging activities with no demonstrable cytotoxicity against Chang liver cells. Conclusions: Five traditional medicinal plants were selected for the present study. The selection of medicinal plants was based on their traditional usage for the treatment of tuberculosis, asthma and chronic respiratory diseases. Herein we report for the first time, the anti TB activity of root extracts of Acacia catechu and Ailanthus excelsa while leaf extract of Andrographis paniculata, Aegle marmelos and Datura metel. The study holds importance in the midst of multi drug resistance (MDR) crisis in the TB management, since it unravels the scientific basis of use of these plant species for the management of TB and related disorders which will be useful for searching the lead compounds from natural products as potential antimycobacterial agents. © 2012 Asian Pacific Tropical Medicine Press. Source


Shinde G.N.,Indira Gandhi Sr College | Mulajkar D.D.,Dnyanasadhana College
Progress in Electromagnetics Research Symposium | Year: 2010

At present, there is a growing interest in designing capacitor-less, resistor-less current mode active only filters using only active elements such as Operational amplifier [OA], Operational transconductance amplifiers [OTAs]. Current mode filters have many advantages compared with their voltage mode counterparts. Current mode filters have large dynamic range, higher bandwidth, greater linearity, simple circuitry, low power consumption etc. A novel single-input current-mode active-R filter using two operational amplifiers (OAs) and resistors is presented. The circuit is fully programmable and implements high pass (HP) functions. The availability of currents at high impedances facilitates cascadibility feature. The filter performance factors center frequency (ω0), bandwidth (ω0/Q), quality factor (Q) and gain (G) are electronically tunable. The SPICE simulation results are included to confirm the workability of the proposed circuit. This circuit can realize quadratic transfer function. Paper includes theoretical frequency response of second order high pass filter for cut off frequency 50 k with variable Q. The circuit is suitable for high frequency operation and monolithic integration. The proposed second order high pass filter works ideal for Q = 10 and central frequency from 1 kHz to 50 kHz. The gain roll-off is 40 dB/decade. The designed filter has passive sensitivities less than unity magnitude and active sensitivities half in magnitude. Source


Bais U.E.,Indira Gandhi Sr College | Lokhande M.Y.,Indira Gandhi Sr College
International Journal of Zoological Research | Year: 2012

Histopathological changes were observed in the gill, liver, stomach and intestine of Ophiocephallus striatus exposed to the cadmium chloride. The fishes were exposed to medinlethal concentration of 0.63 mg L-1 at 96 h. The histopathological change due to the toxicity of cadium chloride in experimental gills of fish observed hypertrophy, destroy of gills lamellae and blood congestion possibly caused the haematological changes in experimental fishes like reduced RBC count, decreased blood hemoglobin and anaemia. In experimental liver of fish observed degenerative changes, necrosis and hypertrophy possibly responsible for biochemical changes like reduced liver glycogen. Hepatocelluar dissociation in experimental fish possibly caused the decreased tissue glycogen and lipid in liver. Degenerative changes (ex., cloudy swelling) possibly caused the endocrine dysfunction leads to increases transaminase activities (hypersecretion of SGOT and SGPT) in present study of haematological study. In experimental stomach of fish hyperchromasia, disintegration of epithelium duct and desquamation of gastric mucosa is observed. Desquamation is a postmortem autolysis which indicates the extremely severe harmful effect of cadmium on the stomach offish. In experimental intestine offish degenerative changes in the tips of villi like hydropic degeneration, cloudy swelling and necrosis is observed the histological changes is possibly due to the fulfillment of extra energy requirement under the toxicity of cadmium chloride. © 2012 Academic Journals Inc. Source

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