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Fondevilla S.,CSIC - Institute for Sustainable Agriculture | Chattopadhyay C.,Indian Institute of Pulses Research | Khare N.,Indira Gandhi Krishi Viswavidyalaya | Rubiales D.,CSIC - Institute for Sustainable Agriculture
European Journal of Plant Pathology | Year: 2013

Until recently, Erysiphe pisi was thought to be the only causal agent of powdery mildew in pea, but recent studies showed that other species such as Erysiphe trifolii and Erysiphe baeumleri can also cause this disease. Three genes, er1, er2 and Er3, conferring resistance to E. pisi have been reported so far in pea. Previous studies showed that E. trifolii and E. baeumleri were able to overcome er1 resistance, but whether er2 and Er3 were effective against E. trifolii was not known. In this study, pea accessions carrying these three genes were evaluated for resistance to E. trifolii under controlled conditions at 20 and 25 °C. In addition, these accessions were also evaluated under field conditions in Spain and in India. Analysis of the ribosomal DNA (rDNA) internal transcribed spacer (ITS) sequences showed that E. trifolii was the causal agent of powdery mildew symptoms in lines carrying er1 in Spain and that this pathogen was also present in India. Our results showed that E. trifolii was able to overcome er1 and shows that this pathogen can also overcome Er3 resistance in some conditions. In contrast, er2 provided high level of resistance against E. trifolii in all conditions and locations studied. Temperature affected the expression of Er3 against E. trifolii, but not of er1 or er2. The pea accession JI2480, containing er2, was highly resistant and JI2302 containing er1 was susceptible to E. trifolii at both temperatures, whereas P660-4 containing Er3 was resistant at 20 °C but susceptible at 25 °C. The present study also identified sources of resistance effective against both E. pisi and E. trifolii. © 2013 KNPV.

De S.,Indira Gandhi Krishi Viswavidyalaya | Sanyal P.K.,Indira Gandhi Krishi Viswavidyalaya | Pan D.,Indira Gandhi Krishi Viswavidyalaya | Bera A.K.,Indira Gandhi Krishi Viswavidyalaya | And 7 more authors.
Indian Journal of Animal Sciences | Year: 2010

The polymerase chain reaction (PCR) method was used to differentiate 4 commonly occurring deuteromycetes (fungi imperfecti), viz. Paecilomyces lilacinus, Verticillium chlamydosporium, Duddingtoniaflagrans and Arthrobotry's oligospora using short sequence oligonucleotide primers. A total of 146 amplicons were identified in the genomic DNA of 4 fungi. Maximum genetic similarity was found in between Paecilomyces lilacinus and Verticilium chlamydosporium (39.96%) while Duddingtoniaflagrans and Verticilium chlamydosporium showed the least (22.5%). Though the fungi are placed in the same group (fungi imperfecti) the results indicate the status of their distinct genotype at molecular level.

Kar A.,Indira Gandhi Krishi Viswavidyalaya | Ganguli J.,Indira Gandhi Krishi Viswavidyalaya
Legume Research | Year: 2016

In a laboratory experiment female Callosobruchus maculatus distributed eggs on healthy chickpea seeds in a manner that maximizes the amount of resources allocated to each offspring under favourable condition. The female preferred seeds having more quantity of resources to meet the nutrition of her offspring, seeds having healthy seed coat over damaged ones, fresh seeds over infested seeds for laying higher number of eggs under normal day light condition over the total dark. This was more so in presence of multiple copulating males over single one. Host deprivation did not have any influence on fecundity. © 2016, Agricultural Research Communication Centre. All rights reserved.

Dash S.P.,Indira Gandhi Krishi Viswavidyalaya | Nair S.K.,Indira Gandhi Krishi Viswavidyalaya | Thakur P.,Indira Gandhi Krishi Viswavidyalaya
Plant Archives | Year: 2015

The present investigation was undertaken to study the mean performance of sweet potato genotypes to assess the cluster analysis in sweet potato for tuber yield and its components at Research and Instructional Farm, Department of Horticulture, Indira Gandhi Krishi Vishwavidyalaya, Raipur (C.G.), India; during the rabi season of 2013-2014. A set of 12 genotypes of sweet potatoes were subjected to D2 analysis for 14 characters and based on D2 values four cluster were formed. No relationship was found between genetic divergence and geographic distribution. TSS and dry matter per cent of tuber contributed high towards total divergence which offered due attention to these characters while selecting for increased tuber yield. The maximum inter cluster distance was observed in between cluster I and IV. The maximum intra cluster distance was observed in cluster III. Hence, genotypes belonging to this cluster viz., Indira Naveen, Sree Rethna, Indira Nandini, Gouri, IGSP-21, IGSP-25, IGSP-39 may be utilized as parent in future breeding programmes with the genotype belonging to cluster IV i.e., IGSP-20 as maximum inter cluster distance.

Dash S.P.,Indira Gandhi Krishi Viswavidyalaya | Singh J.,Indira Gandhi Krishi Viswavidyalaya | Panigrahi T.,Indira Gandhi Krishi Viswavidyalaya | Thakur P.,Indira Gandhi Krishi Viswavidyalaya
Plant Archives | Year: 2015

Understanding interrelationships among various agronomic traits is vital to plan an effective breeding program in sweet potato [Ipomoea batatas (L.) Lam]. This study was undertaken to determine associations among yield and yield related traits. A replicated field experiment was carried out using twelve sweet potato genotypes. Observations were recorded on fourteen characters. Correlation coefficient analysis of tuber yield per hectare expressed a positive significant correlation with biological yield per plant at both genotypic and phenotypic levels whereas, tuber diameter and harvest index showed positive significant correlation at only genotypic level. In path coefficient analysis, biological yield per plant showed maximum positive direct effect on tuber yield (t/ha) followed by vine length, harvest index, neck length of tuber, dry matter per cent of both vine and tuber. Whereas, the characters namely vine weight, vine length, tuber length, inter node length, neck length, tuber diameter and TSS had the highest indirect effect on tuber yield, which ultimately lead to development of high yielding varieties. It could be conclude that due to high estimated positive correlation and positive direct effect of biological yield and harvest index on tuber yield (t/ha), these traits would be most suitable for indirect selection in sweet potato improvement programs that aims to increasing tuber yield.

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