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Patro V.J.,Roland Institute of Pharmaceutical science | Panda C.S.,Indira Gandhi Institute of Pharmaceutical science | Sahoo B.M.,Roland Institute of Pharmaceutical science | Mishra N.K.,Roland Institute of Pharmaceutical science | Panda J.R.,Roland Institute of Pharmaceutical science
Journal of the Indian Chemical Society | Year: 2012

The reaction of 1H-indole-2,3-dione) with substituted anilines in presence of acetic acid produces Schiff bases. Reaction of the Schiff bases with different secondary amines in presence of formaldehyde yields Mannich bases. The synthesized Mannich bases were screened for their antibacterial, analgesic and anti-inflammatory activities by the standard methods. The structures of the compounds were confirmed by means of physical and spectral data. Source


Solanki C.S.,Indira Gandhi Institute of Pharmaceutical science | Tripathy S.,University Technology of MARA | Tripathy M.,University Technology of MARA | Dash U.N.,Siksha O' Anusandhan University
E-Journal of Chemistry | Year: 2010

The present study deals with experiments so as to highlight the solute (drug nimesulide) - solvent(water) interactions and related modifications in case of the presence of hydrotropic agents at different temperatures T(=298.15 to 313.15)K. Density and viscosity values of nimesulide have been determined in water in (0.1, 0.2, 0.4, 0.6, 0.8, 1 and 2) mol dm-3 aqueous solutions of hydrotropic agents (sodium benzoate, sodium salicylate, sodium bromide and nicotinamide) at temperatures 298.15, 303.15, 308.15 and 313.15 K where as the solubility was studied at 308.15 K. From the density values, the limiting partial molar volumes and expansibilities have been calculated. The experimental viscosity values have been analyzed in terms of jones-dole equation and on the basis of transition theory for relative viscosity. Source


Narayan U.L.,Indira Gandhi Institute of Pharmaceutical science | Garnaik B.,Berhampur University
Asian Journal of Pharmaceutical and Clinical Research | Year: 2011

A simple, highly sensitive and accurate method has been developed for the estimation of Nimesulide in liquid dosage form. The proposed method is based on the principle that Nimesulide can exhibit absorption spectra of wavelength maxima at 295 nm in methanol. This method can be successfully used for the analysis of drug in marketed liquid dosage formulations in the range of 10-50μg/ml. The correlation coefficient, percentage of relative standard deviation and percentage recovery was found to be 0.9964, 0.93% and 101.25-106.00% respectively. There was no interference of the excipients. This method have been validated for linearity, accuracy and precision and found to be rapid, precise and economical. Source


Hasnain M.S.,Sri Venkateswara University | Siddiqui S.,Indira Gandhi Institute of Pharmaceutical science | Rao S.,Fortis Clinical Research Ltd. | Mohanty P.,Indira Gandhi Institute of Pharmaceutical science | And 2 more authors.
Journal of Chromatographic Science | Year: 2016

The current studies describe the Quality by Design (QbD)-based development and validation of a LC-MS-MS method for quantification of fluoxetine in human plasma using fluoxetine-D5 as an internal standard (IS). Solid-phase extraction was employed for sample preparation, and linearity was observed for drug concentrations ranging between 2 and 30 ng/mL. Systematic optimization of the method was carried out by employing Box-Behnken design with mobile phase flow rate (X1), pH (X2) and mobile phase composition (X3) as the method variables, followed by evaluating retention time (Rt) (Y1) and peak area (Y2) as the responses. The optimization studies revealed reduction in the variability associated with the method variables for improving the method robustness. Validation studies of the developed method revealed good linearity, accuracy, precision, selectivity and sensitivity of fluoxetine in human plasma. Stability studies performed in human plasma through freeze-thaw, bench-top, short-term and long-term cycles, and autosampler stability revealed lack of any change in the percent recovery of the drug. In a nutshell, the developed method demonstrated satisfactory results for analysis of fluoxetine in human plasma with plausible utility in pharmacokinetic and bioequivalence studies. © 2016 The Author 2016. Published by Oxford University Press. All rights reserved. Source


Narayan U.L.,Indira Gandhi Institute of Pharmaceutical science | Garnaik B.,Berhampur University | Patro S.K.,Institute of Pharmacy and Technology
Research Journal of Pharmaceutical, Biological and Chemical Sciences | Year: 2014

A rapid, simple and sensitive LC-MS/MS analytical method was developed and validated for the determination of voriconazole in human plasma, using Fluconazole as an internal standard. A water Quattro Micro LC-MS/MS was used. Chromatographic separation was achieved using a Vertisep BDS C18 (4.6x 100mm), 5μm, maintained at 35 °C. The samples were eluted using a non-evaporating buffer system; (Phosphate buffer). Desired response was observed for mobile phase, 0.2% formic acid buffer solution: methanol in the ratio of (20:80), at a flow rate of 1 ml/min with a total run time of 2.2 min. The LC system was coupled with an atmospheric pressure ionization source (API-3200) triple quadruple mass spectrometer equipped with an electro spray ionization source, operating in positive mode. Analysis was performed in multiple reaction-monitoring (MRM) mode by monitoring the ion transitions from m/z 350.10→281.10.100 (Voriconazole) and m/z 307.20.→220.20 (IS). Calibration curves in spiked plasma were linear over the concentration range of 25-5000 ng/mL with determination coefficient >0.9989. The lower limit of quantification was 25ng/mL Intra batch and inter batch precision %CV ranged from 0.93% to 5.66% and 3.03% to 5.16%.While % of accuracy was within 92.44-107.61% and 93.69-99.23% respectively. This method has significant advantage over the other reported methods in terms of cost and gave reproducible data with a chromatographic runtime of 2.20 minutes. The reported method provided the necessary sensitivity, linearity, precision, accuracy, and specificity to allow the determination of Voriconazole in pre-clinical pharmacokinetic studies. Source

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