Indira Gandhi Institute of Pharmaceutical science

Bhubaneshwar, India

Indira Gandhi Institute of Pharmaceutical science

Bhubaneshwar, India
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Narayan U.L.,Indira Gandhi Institute of Pharmaceutical science | Garnaik B.,Berhampur University | Patro S.K.,Institute of Pharmacy and Technology
Research Journal of Pharmaceutical, Biological and Chemical Sciences | Year: 2014

A rapid, simple and sensitive LC-MS/MS analytical method was developed and validated for the determination of voriconazole in human plasma, using Fluconazole as an internal standard. A water Quattro Micro LC-MS/MS was used. Chromatographic separation was achieved using a Vertisep BDS C18 (4.6x 100mm), 5μm, maintained at 35 °C. The samples were eluted using a non-evaporating buffer system; (Phosphate buffer). Desired response was observed for mobile phase, 0.2% formic acid buffer solution: methanol in the ratio of (20:80), at a flow rate of 1 ml/min with a total run time of 2.2 min. The LC system was coupled with an atmospheric pressure ionization source (API-3200) triple quadruple mass spectrometer equipped with an electro spray ionization source, operating in positive mode. Analysis was performed in multiple reaction-monitoring (MRM) mode by monitoring the ion transitions from m/z 350.10→281.10.100 (Voriconazole) and m/z 307.20.→220.20 (IS). Calibration curves in spiked plasma were linear over the concentration range of 25-5000 ng/mL with determination coefficient >0.9989. The lower limit of quantification was 25ng/mL Intra batch and inter batch precision %CV ranged from 0.93% to 5.66% and 3.03% to 5.16%.While % of accuracy was within 92.44-107.61% and 93.69-99.23% respectively. This method has significant advantage over the other reported methods in terms of cost and gave reproducible data with a chromatographic runtime of 2.20 minutes. The reported method provided the necessary sensitivity, linearity, precision, accuracy, and specificity to allow the determination of Voriconazole in pre-clinical pharmacokinetic studies.


Narayan U.L.,Indira Gandhi Institute of Pharmaceutical science | Garnaik B.,Berhampur University
Asian Journal of Pharmaceutical and Clinical Research | Year: 2011

A simple, highly sensitive and accurate method has been developed for the estimation of Nimesulide in liquid dosage form. The proposed method is based on the principle that Nimesulide can exhibit absorption spectra of wavelength maxima at 295 nm in methanol. This method can be successfully used for the analysis of drug in marketed liquid dosage formulations in the range of 10-50μg/ml. The correlation coefficient, percentage of relative standard deviation and percentage recovery was found to be 0.9964, 0.93% and 101.25-106.00% respectively. There was no interference of the excipients. This method have been validated for linearity, accuracy and precision and found to be rapid, precise and economical.


Hasnain M.S.,Sri Venkateswara University | Siddiqui S.,Indira Gandhi Institute of Pharmaceutical science | Rao S.,Fortis Clinical Research Ltd | Mohanty P.,Indira Gandhi Institute of Pharmaceutical science | And 2 more authors.
Journal of Chromatographic Science | Year: 2016

The current studies describe the Quality by Design (QbD)-based development and validation of a LC-MS-MS method for quantification of fluoxetine in human plasma using fluoxetine-D5 as an internal standard (IS). Solid-phase extraction was employed for sample preparation, and linearity was observed for drug concentrations ranging between 2 and 30 ng/mL. Systematic optimization of the method was carried out by employing Box-Behnken design with mobile phase flow rate (X1), pH (X2) and mobile phase composition (X3) as the method variables, followed by evaluating retention time (Rt) (Y1) and peak area (Y2) as the responses. The optimization studies revealed reduction in the variability associated with the method variables for improving the method robustness. Validation studies of the developed method revealed good linearity, accuracy, precision, selectivity and sensitivity of fluoxetine in human plasma. Stability studies performed in human plasma through freeze-thaw, bench-top, short-term and long-term cycles, and autosampler stability revealed lack of any change in the percent recovery of the drug. In a nutshell, the developed method demonstrated satisfactory results for analysis of fluoxetine in human plasma with plausible utility in pharmacokinetic and bioequivalence studies. © 2016 The Author 2016. Published by Oxford University Press. All rights reserved.


PubMed | Sri Venkateswara University, Indira Gandhi Institute of Pharmaceutical science, LNM University, Hamdard University and Fortis Clinical Research Ltd
Type: Journal Article | Journal: Journal of chromatographic science | Year: 2016

The current studies describe the Quality by Design (QbD)-based development and validation of a LC-MS-MS method for quantification of fluoxetine in human plasma using fluoxetine-D5 as an internal standard (IS). Solid-phase extraction was employed for sample preparation, and linearity was observed for drug concentrations ranging between 2 and 30 ng/mL. Systematic optimization of the method was carried out by employing Box-Behnken design with mobile phase flow rate (X1), pH (X2) and mobile phase composition (X3) as the method variables, followed by evaluating retention time (Rt) (Y1) and peak area (Y2) as the responses. The optimization studies revealed reduction in the variability associated with the method variables for improving the method robustness. Validation studies of the developed method revealed good linearity, accuracy, precision, selectivity and sensitivity of fluoxetine in human plasma. Stability studies performed in human plasma through freeze-thaw, bench-top, short-term and long-term cycles, and autosampler stability revealed lack of any change in the percent recovery of the drug. In a nutshell, the developed method demonstrated satisfactory results for analysis of fluoxetine in human plasma with plausible utility in pharmacokinetic and bioequivalence studies.


Parida N.K.,Indira Gandhi Institute of Pharmaceutical science | Debata P.C.,Indira Gandhi Institute of Pharmaceutical science | Debata P.C.,Hi Technology Medical College and Hospital | Panda P.K.,Indira Gandhi Institute of Pharmaceutical science | Panda P.K.,Utkal University
Asian Journal of Chemistry | Year: 2010

The neuropharmacological activities of the aqueous extract of Alangium salvifolium (Linn.f.) stem bark extract were screened in rat. The extract effect on pentobarbital-induced sleeping time, pentylenetetrazole induced seizure, spontaneous motor activity (SMA), exploratory behaviour and rota-rod performance (motor coordination) were evaluated. The extract (100 and 200 mg/kg p.o.) produced a significant (p < 0.05) prolongation of pentobarbital-induced sleeping time and reduced the spontaneous motor activity and exploratory behaviour. The extract prolonged onset of the phases of seizure activity but did not protect rats against lethality induced by pentylenetetrazole. It also failed to affect the motor coordination test. These results suggest that the extract contained an agent with neuropharmacological activity that may be sedative in nature.


Meher J.G.,Indira Gandhi Institute of Pharmaceutical science | Meher J.G.,CSIR - Central Electrochemical Research Institute | Tarai M.,Indira Gandhi Institute of Pharmaceutical science | Yadav N.P.,CSIR - Central Electrochemical Research Institute | And 3 more authors.
Carbohydrate Polymers | Year: 2013

The aim of the present work was to develop and characterize mucoadhesive film of cellulose (methyl cellulose and hydroxy propyl methyl cellulose) and polymethacrylate (Eudragit RSPO) polymers for buccal delivery of carvedilol. Drug and polymers were found to be compatible as revealed by FTIR and DSC analysis. Mucoadhesive films were prepared by solvent casting technique. Swelling studies up to 4 h did not show erosion of film, which was further confirmed by SEM analysis. New, simple and precise instrumental methods were established for the evaluation of mucoadhesive strength (33.8 ± 0.37-38.4 ± 0.24 g) and film strength (331.2 ± 0.73-369.0 ± 1.00 g) of developed films. Mucoadhesive film F5 showed 88 ± 1.15% in vitro drug release and 80 ± 2.30% ex vivo drug permeation through goat buccal mucosa in 12 h. Drug release and permeation followed Higuchi's model and mechanism was found to be Fickian type diffusion controlled. © 2013 Elsevier Ltd. All rights reserved.


Solanki C.S.,Indira Gandhi Institute of Pharmaceutical science | Tripathy S.,University Technology of MARA | Tripathy M.,University Technology of MARA | Dash U.N.,Siksha ‘O’ Anusandhan University
E-Journal of Chemistry | Year: 2010

The present study deals with experiments so as to highlight the solute (drug nimesulide) - solvent(water) interactions and related modifications in case of the presence of hydrotropic agents at different temperatures T(=298.15 to 313.15)K. Density and viscosity values of nimesulide have been determined in water in (0.1, 0.2, 0.4, 0.6, 0.8, 1 and 2) mol dm-3 aqueous solutions of hydrotropic agents (sodium benzoate, sodium salicylate, sodium bromide and nicotinamide) at temperatures 298.15, 303.15, 308.15 and 313.15 K where as the solubility was studied at 308.15 K. From the density values, the limiting partial molar volumes and expansibilities have been calculated. The experimental viscosity values have been analyzed in terms of jones-dole equation and on the basis of transition theory for relative viscosity.


Patro V.J.,Roland Institute of Pharmaceutical science | Panda C.S.,Indira Gandhi Institute of Pharmaceutical science | Sahoo B.M.,Roland Institute of Pharmaceutical science | Mishra N.K.,Roland Institute of Pharmaceutical science | Panda J.R.,Roland Institute of Pharmaceutical science
Journal of the Indian Chemical Society | Year: 2012

The reaction of 1H-indole-2,3-dione) with substituted anilines in presence of acetic acid produces Schiff bases. Reaction of the Schiff bases with different secondary amines in presence of formaldehyde yields Mannich bases. The synthesized Mannich bases were screened for their antibacterial, analgesic and anti-inflammatory activities by the standard methods. The structures of the compounds were confirmed by means of physical and spectral data.


Solanki C.S.,Indira Gandhi Institute of Pharmaceutical science | Mishra P.,Indira Gandhi Institute of Pharmaceutical science | Talari M.K.,University Technology of MARA | Tripathy M.,University Technology of MARA | Dash U.N.,Siksha ‘O’ Anusandhan University
E-Journal of Chemistry | Year: 2012

Conductance values of nimesulide have been determined in water in 0.1, 0.2, 0.4, 0.6, 0.8, 1 and 2 mol dm -3 aqueous solutions of hydrotropic agents (sodium benzoate, sodium salicylate, sodium bromide and nicotinamide) at temperatures 298.15, 303.15, 308.15 and 313.15 K. The conductance values have been used to evaluate the limiting molar conductance and association constants by means of Shedlovsky extrapolation technique. Thermodynamic parameters for the association process of nimesulide in aqueous solutions of hydrotropic agents have also been calculated.


PubMed | Indira Gandhi Institute of Pharmaceutical science and CSIR - Central Electrochemical Research Institute
Type: Journal Article | Journal: AAPS PharmSciTech | Year: 2016

The present study aimed to develop buccoadhesive film of glimepiride with unique combination of polymers and to investigate its effect(s) on physicomechanical parameters, drug-release, and permeation of films. Drug-polymer interaction was examined by FTIR and DSC analysis. Films were prepared by solvent casting technique and characterized for film strength (3208.5g, 28.982.00mJ), buccoadhesive strength (28.81.37g, 3.040.32mJ), and tensile strength (2606.88g, 18.000.44mJ) by new instrumental techniques. Increase in polymer concentration augmented zeta potential of polymeric matrix-mucin mixture and exhibited strong buccoadhesion (electrical theory). Buccoadhesion was also influenced by particle size (adsorption theory) and swelling (wetting theory). Erosion behavior of films was observed in swelling and SEM studies. Film GM4 exhibited 982% in vitro drug release and 858% ex vivo drug permeation in 12h with controlled diffusion mechanism. Films were compatible with oral probiotic microorganisms. Stability studies revealed no significant (P<0.05) variation in physicomechanical characteristics.

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