Billeberga, Sweden
Billeberga, Sweden

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Erturk G.,Lund University | Mattiasson B.,Lund University | Mattiasson B.,Indienz AB
Journal of Chromatography A | Year: 2014

Cryogels are polymeric materials formed from monomeric or polymeric precursors in moderately frozen state by polymerization or crosslinking. The process applied for cryogel formation is called cryogelation. These macroporous gel matrices can be produced with different shapes and the gels are of interest in the bioseparation area since they can meet needs that conventional chromatographic media are less suitable to fulfill. High porosity, high mechanical and chemical stability make them appropriate carriers for immobilization of biomolecules and cells thereby making them attractive gel matrices for separation and purification of various molecules. This review highlights the preparation and properties of cryogels, and applications of these materials especially in bioseparation science. © 2014 Elsevier B.V.


Moshi A.P.,Lund University | Moshi A.P.,University of Dar es Salaam | Hosea K.M.M.,University of Dar es Salaam | Elisante E.,University of Dar es Salaam | And 3 more authors.
Bioresource Technology | Year: 2015

The thermoanaerobe, Caloramator boliviensis was used to ferment starch hydrolysate from inedible wild cassava to ethanol at 60°C. A raw starch degrading α-amylase was used to hydrolyse the cassava starch. During fermentation, the organism released CO2 and H2 gases, and Gas Endeavour System was successfully used for monitoring and recording formation of these gaseous products. The bioethanol produced in stoichiometric amounts to CO2 was registered online in Gas Endeavour software and correlated strongly (R2=0.99) with values measured by HPLC. The organism was sensitive to cyanide that exists in cassava flour. However, after acclimatisation, it was able to grow and ferment cassava starch hydrolysate containing up to 0.2. ppm cyanide. The reactor hydrogen partial pressure had influence on the bioethanol production. In fed-batch fermentation by maintaining the hydrogen partial pressure around 590. Pa, the organism was able to ferment up to 76. g/L glucose and produced 33. g/L ethanol. © 2014 Elsevier Ltd.


Asliyuce S.,Hacettepe University | Asliyuce S.,Lund University | Mattiasson B.,Lund University | Mattiasson B.,Indienz AB | And 2 more authors.
Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences | Year: 2016

Monolithic macroporous cryogel imprinted with protein G was prepared using a functional co-monomer of N-methacryloyl-l-phenylalanine and 2-hydroxyethyl methacrylate. The chemical structure of the cryogel prepared was studied by FTIR-spectroscopy and its porosity was analysed using scanning electron microscopy. The cryogel was used to purify protein G from recombinant Escherichia coli cell lysate and the effect of pH, temperature, ionic strength, flow rate, etc on the adsorption of protein G to the monolithic column have been investigated. The selectivity of the imprinted cryogel was studied using protein A and myoglobin. It was possible to capture about 9. mg of Protein G per g of the cryogel. © 2015 Elsevier B.V.


Moshi A.P.,Lund University | Moshi A.P.,University of Dar es Salaam | Temu S.G.,Lund University | Temu S.G.,University of Dar es Salaam | And 6 more authors.
Chemical Engineering Journal | Year: 2015

Cassava peels were pre-treated with alkali, enzyme and in sequential combination of alkali and enzyme, and used for production of bioethanol or biogas, or both (in sequence, bioethanol followed by biogas). The Biogas Endeavour and Automatic Methane Potential Test Systems were used for production of bioethanol and biogas, respectively. The bioethanol yield and volumetric productivity achieved with alkali pre-treatment combined in sequence with enzyme pre-treatment were 1.9. mol/mol and 1.3. g/L/h which was higher than the yield (1.6. mol/mol) and volumetric productivity (0.5. g/L/h) obtained from only enzyme pre-treated peels. Alkali combined in sequence with enzyme was proven to be the best treatment showing a 56% improvement in methane yield compared to the yield from untreated sample. Combined ethanol and methane production resulted in 1.2-1.3-fold fuel energy yield compared to only methane and 3-4-fold compared to only ethanol production. This study therefore provides practical data on the scenario best suited for the harnessing of energy from cassava peels. © 2015 Elsevier B.V.


Moshi A.P.,Lund University | Moshi A.P.,University of Dar es Salaam | Crespo C.F.,Lund University | Crespo C.F.,Higher University of San Andrés | And 7 more authors.
Bioresource Technology | Year: 2014

The objective of this study was to characterise and evaluate a wild inedible cassava species, Manihot glaziovii as feedstock for bioenergy production. Tubers obtained from 3 different areas in Tanzania were characterised and evaluated for bioethanol and biogas production. These bioenergy carriers were produced both separately and sequentially and their energy values evaluated based on these two approaches. Composition analysis demonstrated that M. glaziovii is a suitable feedstock for both bioethanol and biogas production. Starch content ranged from 77% to 81%, structural carbohydrates 3-16%, total crude protein ranged from 2% to 8%. Yeast fermentation achieved ethanol concentration of up to 85 g/L at a fermentation efficiency of 89%. The fuel energy of the bioethanol and methane from flour-peels mix ranged from 5 to 13 and 11 to 14 MJ/kg VS, respectively. Co-production of bioethanol and biogas in which the peels were added to the fermentation residue prior to anaerobic digestion produced maximum fuel energy yield of (15-2 MJ/kg VS). © 2014 Elsevier Ltd.


Mattiasson B.,Indienz AB | Mattiasson B.,Lund University
Advances in Biochemical Engineering/Biotechnology | Year: 2015

Molecular imprints are potentially fantastic constructions. They are selective, robust, and nonbiodegradable if produced from stable polymers. A range of different applications has been presented, everything from separation of enantiomers, via adsorbents for sample preparation before analysis to applications in wastewater treatment. This chapter deals with molecularly imprinted polymers (MIPs) as tools in environmental biotechnology, a field that has the potential to become very important in the future. © 2015, Springer International Publishing Switzerland.

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