Indian Veterinary Research Institute or IVRI is located at Izztnagar, Bareilly in Uttar Pradesh state. It is India's premier advanced research facility in the field of veterinary medicine and allied branches. It has regional campuses at Mukteshwar, Bangalore, Palampur, Bhopal, Kolkata and Srinagar. Formerly known as Imperial Bacteriological Laboratory, it was renamed in 1925 as Imperial Veterinary Research Institute. The name of the Institute was changed following independence to Indian Veterinary Research Institute. Administrative control of the Institute is currently under Indian Council of Agricultural Research , New Delhi. The Ministry of Education, Govt. of India on the recommendation of University Grants Commission conferred the status of the Deemed to be University on 16 November 1983 under Section 3 of UGC Act . Wikipedia.
Shivachandra S.B.,Indian Veterinary Research Institute
Animal health research reviews / Conference of Research Workers in Animal Diseases | Year: 2011
Hemorrhagic septicemia (HS), an acute, fatal and septicemic disease of cattle and buffaloes caused by Pasteurella multocida, is important in tropical regions of the world, especially in African and Asian countries. The prevalence of disease has been well documented with predominant isolation of P. multocida serotypes B:2 and E:2. Conventional methods of identification such as serotyping, biotyping, antibiogram determination and pathogenicity as well as molecular methods (P. multocida-specific polymerase chain reaction (PCR), a serogroup B-specific PCR assay, multiplex capsular typing system and loop-mediated isothermal amplification techniques) and characterization (restriction endonuclease analysis, randomly amplified polymorphic DNA analysis, repetitive extragenic palidromic PCR and enterobacterial repetitive intergenic consensus PCR analysis) are applied in parallel for rapid epidemiological investigations of HS outbreaks. Although several vaccine formulations including alum precipitated, oil adjuvant and multiple emulsion vaccines are commercially available, the quest for suitable broadly protective HS vaccines with long-lasting immunity is on the upsurge. Concurrently, attempts are being made to unravel the mysteries of the pathogen and its virulence factors, pathogenesis and determinants of protective immunity as well as diversity among strains of P. multocida. This review highlights the advances in these various aspects of HS.
Bhanuprakash V.,Indian Veterinary Research Institute
Expert review of vaccines | Year: 2012
The family Poxviridae includes several viruses of medical and veterinary importance. Global concerted efforts combined with an intensive mass-vaccination campaign with highly efficaceious live vaccine of vaccinia virus have led to eradication of smallpox. However, orthopoxviruses affecting domestic animals continue to cause outbreaks in several endemic countries. Different kinds of vaccines starting from conventional inactivated/attenuated to recombinant protein-based vaccines have been used for control of poxvirus infections. Live virus homologous vaccines are currently in use for diseases including capripox, parapox, camelpox and fowlpox, and these vaccines are highly effective in eliciting (with the exception of parapoxviruses) long-lasting immunity. Attenuated strains of poxviruses have been exploited as vectored vaccines to deliver heterologous immunogens, many of them being licensed for use in animals. Worthy of note are vaccinia virus, fowlpox virus, capripoxvirus, parapoxvirus and canary pox, which have been successfully used for developing new-generation vaccines targeting many important pathogens. Remarkable features of these vaccines are thermostability and their ability to engender both cellular and humoral immune responses to the target pathogens. This article updates the important vaccines available for poxviruses of livestock and identifies some of the research gaps in the present context of poxvirus research.
Singh R.P.,Indian Veterinary Research Institute
OIE Revue Scientifique et Technique | Year: 2011
Peste des petits ruminants (PPR) is a contagious viral disease of small ruminants. It is endemic in several African, Middle Eastern and Asian countries, including India. India has recently taken comprehensive steps to deal with PPR through the development and production of potent vaccines and monoclonal-antibody-based diagnostic kits, while also gathering baseline information on the disease situation and human resources. As a result, PPR can now be controlled by focused vaccinations in high-risk populations of sheep and goats, followed by mass vaccination campaigns. Mass vaccination campaigns must achieve high levels of herd immunity (70% to 80%) to block the epidemic cycle of the virus. With the tools currently available, disease control and subsequent eradication programmes for PPR may be a feasible option, following the example of the National Rinderpest Eradication Programme, which has successfully eradicated rinderpest from India. An understanding of the cultural and socio-economic circumstances of goat and sheep owners and a keen watch on the endemic nature of PPR in neighbouring countries will enhance the success of this approach. Coordinated efforts from all stakeholders, combined with proper funding and execution of control programmes, will be needed to achieve the goal of a PPR-free India. In addition, the availability of effective combined vaccines of PPR with goat pox or sheep pox offers a cost-effective way of simultaneously launching control programmes against all three of these diseases.
Das G.K.,Indian Veterinary Research Institute |
Khan F.A.,Indian Veterinary Research Institute
Reproduction in Domestic Animals | Year: 2010
Contents: The present review addresses summer anoestrus in buffaloes. The condition is a major impediment in the improvement of reproductive as well as productive efficiency in buffalo. Factors affecting summer anoestrus include environment, nutrition and management. The environmental factors especially longer day length and increased temperature with high humidity pre-dispose to the condition when the nutritive status of buffaloes is poor. Buffaloes with summer anoestrus fail to exhibit oestrus as a result of aberration in the endocrine profile leading to ovarian inactivity. Increased day length with high environmental temperature causes hyper-prolactinaemia, suppressing the secretion of gonadotrophins, which leads to an alteration in ovarian steroidogenesis. Heat stress produced during summer also affects folliculogenesis, follicular fluid microenvironment and oocyte quality. A large number of hormonal regimens have been used with varying degree of efficacy in terms of oestrus induction and conception rate. A combined strategy of improvement in environment, nutrition and management is pre-requisite for hormonal manipulation in order to improve productivity in summer anoestrus buffaloes. A brief description of summer anoestrus with special reference to factors responsible, endocrinology, deleterious effects on reproductive system and possible remedial measures is presented in this review. © 2010 Blackwell Verlag GmbH.
Uma R.,Indian Veterinary Research Institute
Acta virologica | Year: 2013
This study investigated the anti-neoplastic potential of avian reovirus σC (sigma C) protein on Rous sarcoma virus-induced fibrosarcoma in chicken. The recombinant vector expressing σC protein was injected intra-tumorally into specific pathogen free chicken with fibro-sarcoma at the dose 100μg per bird, while control birds were mock-treated with 100μg of empty vector per bird. Recombinant σC protein induced apoptosis in tumors of treated birds resulting in progressive tumor regression, while similar changes were absent in tumors of mock-treated controls. The σC protein-induced apoptosis in tumors was quantified by flow cytometry and the mean level of apoptosis up to 66% was observed in treated tumors, whereas any significant level of apoptosis was absent in mock-treated controls.
Ghosh S.,Indian Veterinary Research Institute |
Nagar G.,Indian Veterinary Research Institute
Journal of Vector Borne Diseases | Year: 2014
Ticks, as vectors of several zoonotic diseases, are ranked second only to mosquitoes as vectors. The diseases spread by ticks are a major constraint to animal productivity while causing morbidity and mortality in both animals and humans. A number of tick species have been recognised since long as vectors of lethal pathogens, viz. Crimean-Congo haemorrhagic fever virus (CCHFV), Kyasanur forest disease virus (KFDV), Babesia spp, Theileria, Rickettsia conorii, Anaplasma marginale, etc. and the damages caused by them are well-recognised. There is a need to reassess the renewed threat posed by the tick vectors and to prioritize the tick control research programme. This review is focused on the major tick-borne human and animal diseases in India and the progress in vector control research with emphasis on acaricide resistance, tick vaccine and the development of potential phytoacaricides as an integral part of integrated tick control programme. © 2014 Malaria Research Center. All Rights reserved.
Talukder S.,Indian Veterinary Research Institute |
Sharma B.D.,Indian Veterinary Research Institute
Critical Reviews in Food Science and Nutrition | Year: 2015
India stood first for millet production in the world and plays a significant role in meat production and consumption too. To meet the demand of health conscious consumers for healthy and nutritious meat food item, the incorporation of millet grains and its byproducts to the meat products by the processors can serve the purpose. The multidimensional positive nutritional and functional characteristics millet grain not only improve the acceptability of the meat products but also increase its own demand as a main coarse food grain in competition to the wheat and rice over the world. © 2015, Taylor and Francis Group, LLC.
Gokulakrishnan P.,Indian Veterinary Research Institute |
Vergis J.,Indian Veterinary Research Institute
Critical Reviews in Food Science and Nutrition | Year: 2015
Achieving food safety is a global health goal and the food-borne diseases take a major check on global health. Therefore, detection of microbial pathogens in food is the solution to the prevention and recognition of problems related to health and safety. Conventional and standard bacterial detection methods such as culture and colony counting methods and immunology-based methods may take up to several hours or even a few days to yield a result. Obviously, this is inadequate, and recently many researchers are focusing towards the progress of rapid diagnostic methods. The advent of molecular techniques has led to the development of a diverse array of assay for quality control of meat and meat products. Rapid analysis using DNA hybridization and amplification techniques offer more sensitivity and specificity to get results than culture based methods as well as dramatic reduction in the time to get results. Many methods have also achieved the high level automation, facilitating their application as routine sample screening assays. This review is intended to provide an overview of the molecular methods for microbiological quality control of meat and meat products. Copyright © Taylor & Francis Group, LLC.
Khan F.A.,Indian Veterinary Research Institute |
Das G.K.,Indian Veterinary Research Institute
Animal Reproduction Science | Year: 2011
The objective of this study was to evaluate the changes in follicular fluid nitric oxide (NO) and ascorbic acid (AA) concentrations with varying follicle size and functional status, and stage of estrous cycle in buffalo. The main effect of follicle size on NO concentration and size-by-status interaction were statistically significant (P<0.05). Small follicles had a higher (P<0.05) NO concentration compared to medium and large follicles. Further, estrogen-active (EA) small follicles showed increased (P<0.05) NO concentrations than the corresponding estrogen-inactive (EI) follicles. Within EA category, higher (P<0.01) concentrations were recorded in small compared to medium and large follicles. There was no significant main effect of stage (P>0.1) on NO concentration but the stage-by-size interaction was significant (P<0.05) with medium follicles showing a higher (P<0.05) concentration during late luteal stage compared to the mid luteal stage. During early and mid luteal stages, higher (P<0.05) NO concentrations were recorded in small than in medium follicles. A significant (P<0.01) main effect of size on AA concentration was observed with higher values in medium than in small and large follicles. Size-by-status interaction for AA approached statistical significance (P<0.06) with higher (P<0.05) concentrations recorded in medium than in large EA follicles. The main effect of stage on AA concentration was, however, non-significant (P>0.1) but the stage-by-size interaction approached statistical significance (P<0.06) with lower (P<0.05) levels recorded in large compared to medium size follicles during the follicular stage. In conclusion, the present study demonstrates that the concentrations of follicular fluid NO and AA vary according to the follicle size, functional status and stage of estrous cycle suggesting their possible role in process of follicular development during estrous cycle in buffalo. © 2011 Elsevier B.V.
Pramod R.K.,Indian Veterinary Research Institute |
Mitra A.,Indian Veterinary Research Institute
Journal of Assisted Reproduction and Genetics | Year: 2014
Purpose: To develop an efficient protocol for isolation, purification and long-term culture of spermatogonial stem cell (SSC) in goat. Methods: The isolation of SSC was performed by testicular disaggregation by enzymatic digestion using collagenase IV, trypsin and DNase I. Further SSCs were enriched using Percoll density gradient centrifugation. The purity of SSCs was assessed by immunocytochemistry (ICC) using α6 integrin. The SSCs were co-cultured on Sertoli cell feeder layer. The SSC colonies were characterized by studying the expression of SSC specific markers (viz., α6 integrin and PLZF) using ICC. The abundance of mRNAs encoding the markers of SSC (viz., β1 integrin and Oct-4) and Sertoli cells (viz., vimentin) was also assayed using quantitative real-time PCR (qPCR). Results: The viability of isolated testicular cells was > 90 % and the Percoll density gradient method resulted in 3.65 folds enrichment with a purity of 82.5 %. Co-culturing of SSCs with Sertoli cell feeder layer allowed the maintenance of stable SSC colonies even after one and half months of culture. The results of ICC analysis showed the expression of α6 integrin and PLZF in almost all the SSC colonies. qPCR analysis revealed a differential expression of mRNAs encoding β1 integrin, Oct-4 and vimentin markers. Conclusion: Results of this study demonstrate a simple enzymatic digestion and Percoll density gradient method for isolation and enrichment of SSCs, and suitability of Sertoli cell feeder layer for long term in vitro culture of SSC in goats. Results also suggest the possible application of non-caprine antibodies against SSC specific markers for the identification and subsequent assessment of SSCs in goats. © 2014 Springer Science+Business Media.