Indian Institute of Toxicological Research

Lucknow, India

Indian Institute of Toxicological Research

Lucknow, India
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Saxena P.,University of Delhi | Pant V.A.,Dental College and Research Center | Wadhwani K.K.,University of Delhi | Kashyap M.P.,Indian Institute of Toxicological Research | And 2 more authors.
Dental Traumatology | Year: 2011

In vitro experiments were carried out to evaluate the potential of propolis, a natural resin known for its wide therapeutic window, as storage medium to preserve the viability of cultured human periodontal ligament (PDL) cells. Materials and Methods: Primary cultures of human PDL cells were subjected to either independent exposure of propolis (2.5%, 5.0%, 10.0%, and 20.0%), Hank's balanced salt solution (HBSS), milk (0.5%), artificial saliva, Dulbecco's modified Eagle's medium (DMEM) or combination of propolis 10%+DMEM, propolis 20%+DMEM for 30min to 24h at 37°C. Cell viability was assessed using standard endpoints i.e., tetrazolium bromide salt (MTT), neutral red uptake, and trypan blue dye exclusion assay. Results: In general, combinations of propolis 10%+DMEM, propolis 20%+DMEM, and DMEM alone were found to be better than other media used in this study. The difference in the potentials of these media to maintain the cell viability reached to the statistically significant levels by 24h, when compared with other media used viz., propolis 2.5% (P<0.01), propolis 5.0% (P<0.05), propolis 10.0% (P<0.05), propolis 20.0% (P<0.001), HBSS (P<0.001), and milk (P<0.01). Trypan blue dye exclusion assay could be recorded the most sensitive among all the assays selected to study the cell viability of PDL cells. Conclusions: Study indicates that combinations of propolis 10%+DMEM, propolis 20%+DMEM, and DMEM alone are equally good as storage media of choice to keep PDL cells viable during extra-alveolar period up to 24h. Other more readily available medium such as milk may serve as appropriate alternative storage medium for shorter time periods i.e., up to 12h. © 2011 John Wiley & Sons A/S.


Pant V.A.,Babu Banarasi Das University | Pant A.B.,Indian Institute of Toxicological Research | Pant A.B.,Council of Scientific and Industrial Research
Dental Traumatology | Year: 2013

Aim: The resin ionomer Geristore, originally designed for restorative procedures, has been used extensively in treating subgingival defects (such as root resorption and perforations) and as a retrofilling material. The purpose of this study was to evaluate the cell adhesion as well as in vitro biocompatibility of human periodontal fibroblast cells with resin ionomer Geristore in comparison with mineral trioxide aggregate (MTA) and glass ionomer cement (GIC). Material and method: Adhesion, growth, and morphology of human periodontal fibroblasts over test materials were evaluated by scanning electron microscopy (SEM). Biocompatibility was assessed by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide salt (MTT) assay. Results: Compared to glass coverslips, cells grew and spread qualitatively better over the surface of Geristore in comparison with the other test materials. In vitro interpretation indicates that Geristore is significantly less cytotoxic to human periodontal ligament cells. Results of statistical analysis revealed that material extracts had significant effect on cell proliferation at both 24 h (F = 547.62, P < 0.05) and at 48 h (F = 6048.18, P < 0.05). Conclusion: Our study supports that Geristore has enhanced biologic behavior to human periodontal ligament cells and superior biocompatibility in comparison with MTA and GIC, so it can be suggested as a material of choice in root resorption, perforations, and root-end filling. © 2012 John Wiley & Sons A/S.


Tiwari S.,University of Lucknow | Paul B.N.,Indian Institute of Toxicological Research | Kumar S.,AIIMS | Chandra A.,University of Lucknow | And 2 more authors.
Hormone and Metabolic Research | Year: 2014

The present study determines the fat depot-specific expression of leptin and TNF-α and its association with biochemical parameters in postmenopausal women. A total of 108 postmenopausal women were recruited prospectively; 54 were with metabolic syndrome (cases) and 54 were without metabolic syndrome (controls). Leptin and TNF-α mRNA expression in visceral adipose tissue (VAT) and subcutaneous adipose tissue (SAT) were done by Real Time-RT PCR. In cases, the mean (±SD) serum estrogen was significantly lower (41.33±24.90 vs. 23.95±14.45, p<0.001) while leptin (12.85±4.51 vs. 10.34±3.89, p=0.002) and TNF-α (13.81±7.13 vs. 8.00±4.38, p<0.001) were significantly higher as compared to controls. Further, the mean relative VAT mRNA expression of both leptin (0.33±0.29 vs. 0.05±0.09, p<0.001) and TNF-α (0.32±0.31 vs. 0.13±0.09, p<0.001) and expression of SAT leptin (4.91±4.01 vs. 0.50±0.92, p<0.001) also lowered significantly in cases as compared to controls. Further, the relative VAT expression of both leptin (r=-0.32, p<0.001) and TNF-α (r=-0.23, p<0.01) showed significant and negative correlation with glucose; expression of SAT leptin showed significant and positive correlation with HDL (r=0.20, p<0.05) and serum estrogen (r=0.30, p<0.01) while negative correlation with glucose (r=-0.26, p<0.01) and serum TNF-α (r=-0.29, p<0.01); and expression of SAT TNF-α showed significant and positive correlation with insulin (r=0.21, p<0.05) and HOMA (r=0.20, p<0.05). In conclusion, the VAT and SAT leptin mRNA expressions may have a modulatory role in metabolic syndrome. © Georg Thieme Verlag KG Stuttgart · New York.


Saxena S.,University of Lucknow | Srivastava P.,Amity University | Khanna V.K.,Indian Institute of Toxicological Research
Journal of Ocular Pharmacology and Therapeutics | Year: 2010

Purpose: Oxidative damage to cellular membranes plays an important role in the pathobiology of tissue injury. Retinal photoreceptors and platelets are an easy target of oxidants because of high proportion of polyunsaturated fatty acids. A tertiary-care center-based prospective study was undertaken to study the effect of antioxidant supplementation over membrane fluidity in platelets in idiopathic retinal periphlebitis (Eales' disease) for the first time. Methods: Assay of thiobarbituric acid reactive substances (TBARS) levels was done following a standard protocol and membrane fluidity in platelets was estimated using a fluorescent probe, 1,6-diphenyl-1,3,5-hexatrience, in 15 cases and 12 healthy controls. Prednisolone (1mg/kg) in a weekly tapering dose for 6 weeks and a commercially available antioxidant preparation [lutein 3.2mg (containing zeaxanthin 256mcg), l-glutathione 5mg, vitamin E 15IU, vitamin C 150mg, zinc 40mg, copper 2mg, selenium 40mcg, and manganese 5mg] was administered once a day for 3 months to all the cases. Pre-and postantioxidant supplementation platelet TBARS and membrane fluidity levels were assessed in all the cases. Results: Significant increase was observed in TBARS levels in the cases when compared with controls (P=0.01). Platelet fluorescence polarization was significantly higher in cases, indicating decreased membrane fluidity, when compared with controls (P=0.005). Antioxidant supplementation led to marked decrease in TBARS levels (P=0.01) and improved levels of platelet membrane fluidity (P=0.001). Conclusion: Antioxidant supplementation leads to significant decrease in oxidative stress and a significant improvement in platelet membrane fluidity, thereby helping to prevent retinal photoreceptor dysfunction. © 2010, Mary Ann Liebert, Inc.


Saxena S.,University of Lucknow | Srivastava P.,Amity University | Khanna V.K.,Indian Institute of Toxicological Research
Journal of Ocular Biology, Diseases, and Informatics | Year: 2011

Oxidative stress is associated with causation of diabetic vascular complications. A case-control study was undertaken to evaluate the association of platelet thiobarbituric acid reacting substances (TBARS) with the severity of diabetic retinopathy for the first time. Platelet TBARS levels were estimated using standard protocol. Platelet TBARS levels in the cases with non-proliferative diabetic retinopathy, proliferative diabetic retinopathy, and healthy controls were 0.56p̧usmn;0.09, 0.69±0.11 and 0.41p̧usmn;0.1 nmol/h/108 platelets, respectively. A significant increase in platelet TBARS levels was observed in the cases as compared to controls (p<0.001). Elevated TBARS levels were observed to significantly increase further during the proliferative stage of the disease (p<0.01). The increase in platelet TBARS levels, and thereby at retinal level, is associated with angiogenesis in diabetic retinopathy. Supplemental anti-oxidant therapy in diabetic retinopathy may prevent ocular angiogenesis resulting as a consequence of oxidative stress. © Springer Science+Business Media, LLC 2011.


Tiwari A.,University of Lucknow | Tiwari A.,Amity University | Saxena S.,University of Lucknow | Pant A.B.,Indian Institute of Toxicological Research | Srivastava P.,Amity University
Journal of Ocular Biology, Diseases, and Informatics | Year: 2012

Eales' disease is an idiopathic retinal vasculitis of the eye. The disease is predominantly characterized by recurrent vitreous hemorrhage. Interphotoreceptor retinolbinding protein 3 plays a significant role in the etiopathogenesis of this condition. It transports retinoids between the retinal pigment epithelium and the photoreceptors; hence, this protein is a potential target for docking studies. In silico data reveal that herbal molecules interact with regulatory domains of interphotoreceptor retinol-binding protein 3 (IRBP-3), resulting into significant docking score and also forms H-bond and several hydrophobic interactions between active residues of IRBP-3. These interactions between the active residues may lead to significant conformational change in that particular portion of the protein. This efficacy and suitability of ligand was determined on the basis of binding energy calculations. Ginkgolide showed minimum binding energy calculations among selected 10 other natural ligands. This fact of virtual screening for potential ligand can give new insights toward the therapeutic intonations and alterations toward the advances in treatment for Eales' disease. © Springer Science+Business Media New York 2013.


Gajbhiye T.,Guru Ghasidas University | Pandey S.K.,Guru Ghasidas University | Kim K.-H.,Hanyang University | Szulejko J.E.,Hanyang University | Prasad S.,Indian Institute of Toxicological Research
Science of the Total Environment | Year: 2016

In order to investigate possible foliar transfer of toxic heavy metals, concentrations of Cd, Pb, and Fe were measured in samples of: Cassia siamea leaves (a common tree) Cassia siamea foliar dust, nearby road dust, and soil (Cassia siamea tree roots) at six different sites in/around the Bilaspur industrial area and a control site on the university campus. Bilaspur is located in a subtropical central Indian region. The enrichment factor (EF) values of Pb and Cd, when derived using the crustal and measured soil Fe data as reference, indicated significant anthropogenic contributions to Pb and Cd regional pollution. Based on correlation analysis and scanning electron microscopy (SEM) observations, it was evident that Pb and Cd in foliar part of Cassia siamea were largely from airborne sources. The SEM studies of leaf confirmed that leaf morphology (epidermis, trichome, and stomata) of Cassia siamea helped accumulate the toxic metals from deposited particulate matter (PM). There is a line of evidence that the leaf of Cassia siamea was able to entrap PM in respirable suspended particulate matter (RSPM) range (i.e., both in fine and coarse fractions). The overall results of this study suggest that Cassia siamea can be a potential plant species to control the pollution of PM and PM-bound metals (Pb and Cd) in affected areas. © 2016 Elsevier B.V.


PubMed | Indian Institute of Toxicological Research, Hanyang University and Guru Ghasidas University
Type: | Journal: The Science of the total environment | Year: 2016

In order to investigate possible foliar transfer of toxic heavy metals, concentrations of Cd, Pb, and Fe were measured in samples of: Cassia siamea leaves (a common tree) Cassia siamea foliar dust, nearby road dust, and soil (Cassia siamea tree roots) at six different sites in/around the Bilaspur industrial area and a control site on the university campus. Bilaspur is located in a subtropical central Indian region. The enrichment factor (EF) values of Pb and Cd, when derived using the crustal and measured soil Fe data as reference, indicated significant anthropogenic contributions to Pb and Cd regional pollution. Based on correlation analysis and scanning electron microscopy (SEM) observations, it was evident that Pb and Cd in foliar part of Cassia siamea were largely from airborne sources. The SEM studies of leaf confirmed that leaf morphology (epidermis, trichome, and stomata) of Cassia siamea helped accumulate the toxic metals from deposited particulate matter (PM). There is a line of evidence that the leaf of Cassia siamea was able to entrap PM in respirable suspended particulate matter (RSPM) range (i.e., both in fine and coarse fractions). The overall results of this study suggest that Cassia siamea can be a potential plant species to control the pollution of PM and PM-bound metals (Pb and Cd) in affected areas.


Sadashiv,University of Lucknow | Tiwari S.,University of Lucknow | Paul B.N.,Indian Institute of Toxicological Research | Kumar S.,University of Lucknow | And 3 more authors.
Women's Health | Year: 2012

Aim: The present study evaluates resistin mRNA expression in visceral adipose tissue (VAT) and its correlation with insulin resistance (homeostatic model assessment) in postmenopausal obese women. Materials & methods: A total of 68 (nonobese = 34 and obese = 34) age-matched (49-70 years) postmenopausal women were recruited for the study. Fasting blood samples were collected at admission and abdominal VAT were obtained during surgery for gall bladder stones or hysterectomy. Physical parameters (age, height, weight and BMI) were measured. Biochemical parameters (plasma insulin, plasma glucose and serum resistin) were estimated by enzymatic methods. The VAT resistin mRNA expression was evaluated by real-time PCR. Results: The relative mean (± standard deviation) VAT resistin mRNA expression in postmenopausal obese women lowered significantly by 20.4% compared with postmenopausal nonobese women (0.029 ± 0.011 vs 0.023 ± 0.013; p = 0.047). Furthermore, VAT resistin mRNA expression in postmenopausal obese women was downregulated by 0.69-fold when compared with age-matched postmenopausal nonobese women. Furthermore, the relative VAT resistin mRNA expression in postmenopausal obese women showed significant inverse association with insulin resistance (r = -0.48; p < 0.01) and serum resistin (r = -0.84; p < 0.001), while in postmenopausal nonobese women it did not show any association with both insulin resistance (r = 0.03; p > 0.05) and serum resistin (r = -0.03; p > 0.05). Conclusion: The VAT resistin mRNA expression in postmenopausal obese women is associated to insulin resistance. © 2012 Future Medicine Ltd.


Harjai M.,Chhatrapati Shahuji Maharaj Medical University | Bogra J.,Chhatrapati Shahuji Maharaj Medical University | Kohli M.,Chhatrapati Shahuji Maharaj Medical University | Pant A.B.,Chhatrapati Shahuji Maharaj Medical University | Pant A.B.,Indian Institute of Toxicological Research
Anaesthesia and Intensive Care | Year: 2013

Sepsis remains as a leading cause of death in critically ill patients. Unfortunately, there have been very few successful specific therapeutic agents that can significantly reduce the attributable mortality and morbidity of sepsis. Developing novel therapeutic strategies to improve outcomes of sepsis remains an important focus of ongoing research in the field of critical care medicine. Apoptosis has recently been identified as an important mechanism of cell death and evidence suggests that prevention of cell apoptosis can improve survival in animal models of sepsis and endotoxaemia. In this review article, we summarise the critical role of apoptosis of the immune cells in the pathophysiology of sepsis and propose that blocking cell-signaling pathways leading to apoptosis may present a promising specific therapy for sepsis. Various methods to inhibit apoptosis including the cell surface Fas receptor pathway inhibitors, caspase inhibitors, over-expression of anti-apoptotic genes and small interfering ribonucleic acid therapy are discussed.

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