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Saxena S.,University of Lucknow | Srivastava P.,Amity University | Khanna V.K.,Indian Institute of Toxicological Research
Journal of Ocular Pharmacology and Therapeutics | Year: 2010

Purpose: Oxidative damage to cellular membranes plays an important role in the pathobiology of tissue injury. Retinal photoreceptors and platelets are an easy target of oxidants because of high proportion of polyunsaturated fatty acids. A tertiary-care center-based prospective study was undertaken to study the effect of antioxidant supplementation over membrane fluidity in platelets in idiopathic retinal periphlebitis (Eales' disease) for the first time. Methods: Assay of thiobarbituric acid reactive substances (TBARS) levels was done following a standard protocol and membrane fluidity in platelets was estimated using a fluorescent probe, 1,6-diphenyl-1,3,5-hexatrience, in 15 cases and 12 healthy controls. Prednisolone (1mg/kg) in a weekly tapering dose for 6 weeks and a commercially available antioxidant preparation [lutein 3.2mg (containing zeaxanthin 256mcg), l-glutathione 5mg, vitamin E 15IU, vitamin C 150mg, zinc 40mg, copper 2mg, selenium 40mcg, and manganese 5mg] was administered once a day for 3 months to all the cases. Pre-and postantioxidant supplementation platelet TBARS and membrane fluidity levels were assessed in all the cases. Results: Significant increase was observed in TBARS levels in the cases when compared with controls (P=0.01). Platelet fluorescence polarization was significantly higher in cases, indicating decreased membrane fluidity, when compared with controls (P=0.005). Antioxidant supplementation led to marked decrease in TBARS levels (P=0.01) and improved levels of platelet membrane fluidity (P=0.001). Conclusion: Antioxidant supplementation leads to significant decrease in oxidative stress and a significant improvement in platelet membrane fluidity, thereby helping to prevent retinal photoreceptor dysfunction. © 2010, Mary Ann Liebert, Inc. Source


Saxena S.,University of Lucknow | Srivastava P.,Amity University | Khanna V.K.,Indian Institute of Toxicological Research
Journal of Ocular Biology, Diseases, and Informatics | Year: 2011

Oxidative stress is associated with causation of diabetic vascular complications. A case-control study was undertaken to evaluate the association of platelet thiobarbituric acid reacting substances (TBARS) with the severity of diabetic retinopathy for the first time. Platelet TBARS levels were estimated using standard protocol. Platelet TBARS levels in the cases with non-proliferative diabetic retinopathy, proliferative diabetic retinopathy, and healthy controls were 0.56p̧usmn;0.09, 0.69±0.11 and 0.41p̧usmn;0.1 nmol/h/108 platelets, respectively. A significant increase in platelet TBARS levels was observed in the cases as compared to controls (p<0.001). Elevated TBARS levels were observed to significantly increase further during the proliferative stage of the disease (p<0.01). The increase in platelet TBARS levels, and thereby at retinal level, is associated with angiogenesis in diabetic retinopathy. Supplemental anti-oxidant therapy in diabetic retinopathy may prevent ocular angiogenesis resulting as a consequence of oxidative stress. © Springer Science+Business Media, LLC 2011. Source


Tiwari S.,University of Lucknow | Paul B.N.,Indian Institute of Toxicological Research | Kumar S.,AIIMS | Chandra A.,University of Lucknow | And 2 more authors.
Hormone and Metabolic Research | Year: 2014

The present study determines the fat depot-specific expression of leptin and TNF-α and its association with biochemical parameters in postmenopausal women. A total of 108 postmenopausal women were recruited prospectively; 54 were with metabolic syndrome (cases) and 54 were without metabolic syndrome (controls). Leptin and TNF-α mRNA expression in visceral adipose tissue (VAT) and subcutaneous adipose tissue (SAT) were done by Real Time-RT PCR. In cases, the mean (±SD) serum estrogen was significantly lower (41.33±24.90 vs. 23.95±14.45, p<0.001) while leptin (12.85±4.51 vs. 10.34±3.89, p=0.002) and TNF-α (13.81±7.13 vs. 8.00±4.38, p<0.001) were significantly higher as compared to controls. Further, the mean relative VAT mRNA expression of both leptin (0.33±0.29 vs. 0.05±0.09, p<0.001) and TNF-α (0.32±0.31 vs. 0.13±0.09, p<0.001) and expression of SAT leptin (4.91±4.01 vs. 0.50±0.92, p<0.001) also lowered significantly in cases as compared to controls. Further, the relative VAT expression of both leptin (r=-0.32, p<0.001) and TNF-α (r=-0.23, p<0.01) showed significant and negative correlation with glucose; expression of SAT leptin showed significant and positive correlation with HDL (r=0.20, p<0.05) and serum estrogen (r=0.30, p<0.01) while negative correlation with glucose (r=-0.26, p<0.01) and serum TNF-α (r=-0.29, p<0.01); and expression of SAT TNF-α showed significant and positive correlation with insulin (r=0.21, p<0.05) and HOMA (r=0.20, p<0.05). In conclusion, the VAT and SAT leptin mRNA expressions may have a modulatory role in metabolic syndrome. © Georg Thieme Verlag KG Stuttgart · New York. Source


Pant V.A.,Babu Banarasi Das University | Pant A.B.,Indian Institute of Toxicological Research | Pant A.B.,Council of Scientific and Industrial Research
Dental Traumatology | Year: 2013

Aim: The resin ionomer Geristore, originally designed for restorative procedures, has been used extensively in treating subgingival defects (such as root resorption and perforations) and as a retrofilling material. The purpose of this study was to evaluate the cell adhesion as well as in vitro biocompatibility of human periodontal fibroblast cells with resin ionomer Geristore in comparison with mineral trioxide aggregate (MTA) and glass ionomer cement (GIC). Material and method: Adhesion, growth, and morphology of human periodontal fibroblasts over test materials were evaluated by scanning electron microscopy (SEM). Biocompatibility was assessed by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide salt (MTT) assay. Results: Compared to glass coverslips, cells grew and spread qualitatively better over the surface of Geristore in comparison with the other test materials. In vitro interpretation indicates that Geristore is significantly less cytotoxic to human periodontal ligament cells. Results of statistical analysis revealed that material extracts had significant effect on cell proliferation at both 24 h (F = 547.62, P < 0.05) and at 48 h (F = 6048.18, P < 0.05). Conclusion: Our study supports that Geristore has enhanced biologic behavior to human periodontal ligament cells and superior biocompatibility in comparison with MTA and GIC, so it can be suggested as a material of choice in root resorption, perforations, and root-end filling. © 2012 John Wiley & Sons A/S. Source


Tiwari A.,University of Lucknow | Trivedi A.C.,Amity University | Srivastava P.,Amity University | Pant A.B.,Indian Institute of Toxicological Research | Saxena S.,University of Lucknow
Journal of Ocular Biology, Diseases, and Informatics | Year: 2011

Retinal S-antigen and interphotoreceptor retinoidbinding protein-3 play a significant role in the etiopathogenesis of Eales' disease. Protein 3D structures are functionally very important and play a significant role in progression of the disease, hence these 3D structures are better target for further drug designing and relative studies. We developed 3D model structure of retinol-binding protein-3 and retinal S-antigen protein of human involved in Eales' disease. Functional site prediction is a very important and related step; hence, in the current course of analysis, we predicted putative functional site residues in the target proteins. Molecular models of these proteins of Eales' disease as documented in this study may provide a valuable aid for designing an inhibitor or better ligand against Eales' disease and could play a significant role in drug design. © Springer Science+Business Media, LLC 2011. Source

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