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Kanpur, India

Indian Institute of Pulses Research is a government institute in Kanpur, Uttar Pradesh. It was established in the year 1983 by the Indian Council of Agricultural Research to carry out basic strategic and applied research on major pulse crops. It is situated on Grand Trunk Road and is about twelve kilometer from Kanpur Central Railway Station towards New Delhi. The overall climate varies from semi-arid to sub-humid and mean annual rainfall ranges from 800 to 1000mm. Wikipedia.

Devraj,Indian Institute of Pulses Research | Jain R.,CSJM University
Expert Systems with Applications

This paper presents design and development of an expert system for the diagnosis and control of diseases in pulse crops (PulsExpert). PulsExpert is an operational automatic diagnostic tool that helps farmers/extension workers to identify diseases of major pulse crops viz.; Chickpea, Pigeonpea, Mungbean and Urdbean (highly consumed pulse crops) and suggests the appropriate control measures. Automatic knowledge acquisition system of PulsExpert provides user-friendly interface to the domain experts for entering, storing and structuring the domain specific knowledge. The knowledge base has been designed after examining the type and structure of the knowledge from different sources like literatures, books, databases, farmers, extension workers, etc. For a particular crop, knowledge can be entered by more than one expert using an automatic knowledge acquisition system and system automatically integrates the knowledge to build a consistent knowledge base. The knowledge base of PulsExpert contains up-to-date knowledge about 19 major diseases of pulses appearing right from seedling to maturity. The system provides user-friendly interface to farmers and asks the textual as well as pictorial questions. The order of questions to be asked is decided dynamically depending upon the answers of the farmer. On the basis of answers, PulsExpert diagnosis the pulse crop diseases along with its confidence factor and suggests most appropriate control measures which are composed of cultural practices as well as chemical controls. PulsExpert was evaluated by a team of field farmers and State Agriculture Officers and it was considered good with an average rank of 2.745 by farmers and 2.075 by State Agriculture Officers with a statistic mode ranking 3 in both the cases. © 2011 Elsevier Ltd. All rights reserved. Source

Singh D.,Central Agricultural University | Choudhary A.K.,Indian Institute of Pulses Research
Plant Breeding

An understanding of the inheritance of aluminum (Al) tolerance is important to breed for Al tolerant genotypes of pea (Pisum sativum L.). Therefore, an investigation was undertaken to infer genes governing Al tolerance in pea. To study the inheritance of Al tolerance, tolerant lines 'Azad P1' and 'PC-55-11-1-2' were crossed with sensitive lines 'PC-493-5' and 'PSM-2'. Parental, F 1, F 2 and backcross generations were grown in a nutrient solution containing 30 ppm of Al for haematoxylin staining and root regrowth and classified for tolerance by staining of root tips and root regrowth. The F 1 hybrids responded similarly to the tolerant parents indicating dominance of Al tolerance over sensitivity. Segregation for tolerance vs. sensitivity in F 2 fitted well with the 3-1 ratio expected for a single gene. The backcrosses involving dominant parents showed the dominant reaction on all the plants while those involving susceptible parents segregated into one tolerant to one sensitive ratio. Experimental results showed that Al tolerance is a monogenic dominant trait that can be easily transferred to desirable lines through backcross breeding programme. © 2010 Blackwell Verlag GmbH. Source

Gupta O.P.,Directorate of Wheat Research | Meena N.L.,Directorate of Wheat Research | Meena N.L.,Indian Institute of Pulses Research | Sharma I.,Directorate of Wheat Research | Sharma P.,Directorate of Wheat Research
Molecular Biology Reports

MicroRNAs (miRNAs) are tiny non-coding regulatory molecules that modulate plant's gene expression either by cleaving or repressing their mRNA targets. To unravel the plant actions in response to various environmental factors, identification of stress related miRNAs is essential. For understanding the regulatory behaviour of various abiotic stresses and miRNAs in wheat genotype C-306, we examined expression profile of selected conserved miRNAs viz. miR159, miR164, miR168, miR172, miR393, miR397, miR529 and miR1029 tangled in adapting osmotic, salt and cold stresses. The investigation revealed that two miRNAs (miR168, miR397) were down-regulated and miR172 was up-regulated under all the stress conditions. However, miR164 and miR1029 were up-regulated under cold and osmotic stresses in contrast to salt stress. miR529 responded to cold alone and does not change under osmotic and salt stress. miR393 showed up-regulation under osmotic and salt, and down-regulation under cold stress indicating auxin based differential cold response. Variation in expression level of studied miRNAs in presence of target genes delivers a likely elucidation of miRNAs based abiotic stress regulation. In addition, we reported new stress induced miRNAs Ta-miR855 using computational approach. Results revealed first documentation that miR855 is regulated by salinity stress in wheat. These findings indicate that diverse miRNAs were responsive to osmotic, salt and cold stress and could function in wheat response to abiotic stresses. © 2014 Springer Science+Business Media. Source

Choudhary A.K.,Indian Institute of Pulses Research
Indian Journal of Agricultural Sciences

Pigeonpea is the second important grain legume of India. In north-east plains, long-duration pigeonpea is grown almost exclusiely as an intercrop with other tall cereals. Development of wilt-resistant lines is the major objective to ensure stability in pigeonpea production and productivity. One genotype 'IPA 204' of long-duration pigeonpea was developed following pedigree method. This high-yielding line showed resistant to moderately resistant wilt reaction at 24 locations spread across arious pigeonpea-growing regions of the country. Therefore. it could be considered resistant to all the reported 5 strains of F. udurn. Keeping its stability of resistance for the last 4 years, it was recommended as the potential donor for wilt resistance in the Annual Group Meet on Pigeonpea (2009). Source

Agbagwa I.O.,Indian Institute of Pulses Research
Genetics and molecular research : GMR

Current DNA extraction protocols, which require liquid nitrogen, lyophilization and considerable infrastructure in terms of instrumentation, often impede the application of biotechnological tools in less researched crops in laboratories in developing countries. We modified and optimized the existing CTAB method for plant genomic DNA extraction by avoiding liquid nitrogen usage and lyophilization. DNA was extracted directly from freshly harvested leaves ground in pre-heated CTAB buffer. Chloroform:isoamyl alcohol (24:1) and RNase treatments followed by single-purification step decontaminated the samples thereby paving way for selective extraction of DNA. High molecular weight DNA yield in the range of 328 to 4776 ng/μL with an average of 1459 ng/μL was obtained from 45 samples of cultivated and wild Cajanus species. With an absorbance ratio at 260 to 280 nm, a range of 1.66 to 2.20, and a mean of 1.85, very low levels of protein and polysaccharide contamination were recorded. Forty samples can be extracted daily at a cost between 1.8 and US$2.0 per plant sample. This modified method is suitable for most plants especially members of the Leguminosae. Apart from Cajanus, it has been extensively applied in DNA extraction from Cicer and Vigna species. Source

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